广西巴马小型猪ApoA1基因克隆及其生物信息学分析
|
摘要
【目的】克隆广西巴马小型猪载脂蛋白A1(ApoA1)基因并进行生物信息学分析,为后续开展ApoA1基因研究提供基础数据,同时为制作广西巴马小型猪动脉粥样硬化疾病模型打下基础。【方法】以提取的广西巴马小型猪肝脏组织总RNA为模板,运用RT-PCR克隆广西巴马小型猪ApoA1基因序列,以实时荧光定量PCR(qPCR)检测ApoA1基因在广西巴马小型猪心脏、肝脏、脾脏、肺脏和肾脏组织中的表达情况,并利用MegAlign、ProtParam、SOPMA、NetPhos、SignalP和SWISS-MODEL等在线分析软件对广西巴马小型猪ApoA1基因进行生物信息学分析及构建系统发育进化树。【结果】广西巴马小型猪ApoA1基因编码区(CDS)序列长795 bp,与普通猪ApoA1基因序列的同源性为99.9%,仅第630处有1个碱基发生同义突变。ApoA1基因在广西巴马小型猪心脏、肝脏、脾脏、肺脏和肾脏组织中均有表达,且以肝脏中的相对表达量最高、脾脏中的相对表达量最低。广西巴马小型猪ApoA1由264个氨基酸残基构成,其分子式为C1343H2136N376O409S5,蛋白分子量为30254.31 Da,理论等电点(pI)为5.47,属于亲水性蛋白,存在跨膜结构,不存在信号肽,有18处磷酸化位点(丝氨酸磷酸化位点有10处,苏氨酸磷酸化位点有6处,络氨酸磷酸化位点有2处)。广西巴马小型猪ApoA1的二级结构中,α-螺旋占79.92%,β-折叠占2.65%,无规则卷曲占11.12%,延伸链占6.31%,属于全α类蛋白。广西巴马小型猪ApoA1氨基酸序列与普通猪的同源性最高,达99.6%;由基于ApoA1氨基酸序列同源性构建的系统发育进化树也可看出,广西巴马小型猪与普通猪的亲缘关系最近。【结论】广西巴马小型猪ApoA1基因保守性强,以在肝脏中的表达量最高,是广西巴马小型猪抗动脉粥样硬化的重要因子,通过敲除该基因可制作广西巴马小型猪动脉粥样硬化疾病模型。
【Objective】The aim of this study was to clone the apoliprotein A1(ApoA1)gene of Guangxi Bama minipig,and analyze the biological information of ApoA1 gene,which would enrich the basic data for the follow-up study of ApoA1 gene and lay a foundation for the production of the atherosclerosis disease model of Guangxi Bama mini-pig.【Method】Total RNA extracted from the heart and liver of Guangxi Bama mini-pig was as template,and the sequence of ApoA1 gene was cloned by RT-PCR. The expression of ApoA1 gene in heart,liver,spleen,lung and kidney of Guangxi Bama mini-pig was detected by real-time fluorescence quantitative(qPCR). Online analysis softwares including MegAlign,ProtParam,SOPMA,NetPhos,SignalP and SWISS-MODEL were used to analyze the bioinformatics of ApoA1 gene and construct phylogenetic tree.【Result】The length of coding sequence(CDS)region of ApoA1 gene of Guangxi Bama mini-pig was 795 bp and the homology was 99.9% compared with the ApoA1 gene sequence of pigs with a synonymy mutation on one base at the 630 position. The ApoA1 gene was expressed in heart,liver,spleen,lung and kidney of Guangxi Bama mini-pig with the highest expression in liver and the lowest expression in spleen.The molecular formula of ApoA1 gene encoding 264 amino acid residues in Guangxi Bama mini-pig was C1343 H2136 N376 O409 S5,the molecular weight was 30254.31 Da,and the theoretical pI was 5.47,belonging to hydrophilic protein. There was transmembrane structure,no signal peptide. There were 18 phosphorylation sites,including 10 sites of serine,6 sites of threonine and 2 sites of tyrosine. The secondary structure of coding protein belonged to all-alpha protein,which consisted of α-helix,β-fold,random coil and extended strand,accounting for 79.92%,2.65%,11.12% and 6.31%,respectively. It was found that the homology of ApoA1 amino acid sequence between Guangxi Bama mini-pig and ordinary pig was the highest(99.6%). According to phylogenetic tree based on ApoA1 amino acid sequence homology,Guangxi Bama mini-pig had the closest genetic relationship with pig.【Conclusion】The sequence of ApoA1 gene in Guangxi Bama mini-pig is strongly conservative and expresses the highest in liver. It is an important factor of anti-atherosclerosis in Guangxi Bama mini-pig. The model of atherosclerotic disease in Guangxi Bama mini-pig can be produced by knocking out the ApoA1 gene.
【Objective】The aim of this study was to clone the apoliprotein A1(ApoA1)gene of Guangxi Bama minipig,and analyze the biological information of ApoA1 gene,which would enrich the basic data for the follow-up study of ApoA1 gene and lay a foundation for the production of the atherosclerosis disease model of Guangxi Bama mini-pig.【Method】Total RNA extracted from the heart and liver of Guangxi Bama mini-pig was as template,and the sequence of ApoA1 gene was cloned by RT-PCR. The expression of ApoA1 gene in heart,liver,spleen,lung and kidney of Guangxi Bama mini-pig was detected by real-time fluorescence quantitative(qPCR). Online analysis softwares including MegAlign,ProtParam,SOPMA,NetPhos,SignalP and SWISS-MODEL were used to analyze the bioinformatics of ApoA1 gene and construct phylogenetic tree.【Result】The length of coding sequence(CDS)region of ApoA1 gene of Guangxi Bama mini-pig was 795 bp and the homology was 99.9% compared with the ApoA1 gene sequence of pigs with a synonymy mutation on one base at the 630 position. The ApoA1 gene was expressed in heart,liver,spleen,lung and kidney of Guangxi Bama mini-pig with the highest expression in liver and the lowest expression in spleen.The molecular formula of ApoA1 gene encoding 264 amino acid residues in Guangxi Bama mini-pig was C1343 H2136 N376 O409 S5,the molecular weight was 30254.31 Da,and the theoretical pI was 5.47,belonging to hydrophilic protein. There was transmembrane structure,no signal peptide. There were 18 phosphorylation sites,including 10 sites of serine,6 sites of threonine and 2 sites of tyrosine. The secondary structure of coding protein belonged to all-alpha protein,which consisted of α-helix,β-fold,random coil and extended strand,accounting for 79.92%,2.65%,11.12% and 6.31%,respectively. It was found that the homology of ApoA1 amino acid sequence between Guangxi Bama mini-pig and ordinary pig was the highest(99.6%). According to phylogenetic tree based on ApoA1 amino acid sequence homology,Guangxi Bama mini-pig had the closest genetic relationship with pig.【Conclusion】The sequence of ApoA1 gene in Guangxi Bama mini-pig is strongly conservative and expresses the highest in liver. It is an important factor of anti-atherosclerosis in Guangxi Bama mini-pig. The model of atherosclerotic disease in Guangxi Bama mini-pig can be produced by knocking out the ApoA1 gene.
引文
陈东,严激.2010.动脉粥样硬化中基质金属蛋白酶的研究进展[J].安徽医药,14(8):874-876.[Chen D,Yan J.2010.Progress of matrix metalloproteinase research in atherosclerosis[J].Anhui Medical and Pharmaceutical Journal,14(8):874-876.]
阚延泽,江翱,孙文秀,李伟.2016.黄鳝载脂蛋白A1的基因结构及表达谱分析[J].水产科学,35(6):697-701.[Kan Y Z,Jiang A,Sun W X,Li W.2016.Gene structure and expression of ApoA1 gene from swamp eel[J].Fisheries Science,35(6):697-701.]
雷蕾,陈丽.2011.血浆载脂蛋白与冠心病的相关性研究[J].当代医学,17(12):13-14.[Lei L,Chen L.2011.Study on the correlation between plasma apolipoprotein and coronary heart disease[J].Contemporary Medicine,17(12):13-14.]
李芳芳,郭亚芬,兰干球,潘堂峰.2014.广西巴马小型猪GHRH成熟肽真核表达质粒对小鼠生长效应的影响[J].南方农业学报,45(12):2264-2269.[Li F F,Guo Y F,Lan G Q,Pan T F.2014.Eukaryotic expression plasmid of GHRH mature peptide from Guangxi Bama Mini-pig and its influence on mouse growth effect[J].Journal of Southern Agriculture,45(12):2264-2269.
李龙,司景磊,夏攀洁,何剑雄,吴延军,程晓芳,吴敏,徐文文,兰干球.2017.广西巴马小型猪Neuritin基因克隆与生物信息学分析及真核表达载体的构建[J].基因组学与应用生物学,36(2):557-563.[Li L,Si J L,Xia P J,He J X,Wu Y J,Cheng X F,Wu M,Xu W W,Lan GQ.2017.Cloning and bioinformatics analysis of Neuritin gene from Guangxi Bama Mini-pig and construction of its eukaryotic expression vector[J].Genomics and Applied Biology,36(2):557-563.]
刘培彬,叶子璐,蔡潭溪,戴绍军,杨福全.2014.脂蛋白质组学研究进展[J].生物化学与生物物理进展,41(12):1197-1206.[Liu P B,Ye Z L,Cai T X,Dai S J,Yang F Q.2014.The research advances in lipoproteinomics[J].Progress in Biochemistry and Biophysics,41(12):1197-1206.]
麻延峰,方倩倩,王宏艳,田勇,唐军旺,邵荣益,卢立志.2015.鹅ApoA1、CD36和DGAT2基因的cDNA克隆与序列分析[J].浙江农业学报,27(10):1710-1719.[Ma Y F,Fang Q Q,Wang H Y,Tian Y,Tang J W,Shao R Y,Lu L Z.2015.cDNA cloning and sequence analysis of ApoA1,CD36 and DGAT2 genes from goose[J].Acta Agriculturae Zhejiangensis,27(10):1710-1719.]
申玉建,方程,邱庆庆,司景磊,吴延军,杨祝良,杨秀荣,郭亚芬,蒋和生.2017.广西巴马小型猪PPARγ基因克隆及组织表达差异分析[J].南方农业学报,48(10):1884-1890.[Shen Y J,Fang C,Qiu Q Q,Si J L,Wu Y J,Yang Z L,Yang X R,Guo Y F,Jiang H S.2017.Cloning of gene PPARγfrom Guangxi Bama mini-pig and tissue expression difference analysis for it[J].Journal of Southern Agriculture,48(10):1884-1890.]
宋少锐,吴延军,蒋世强,张名媛,郭亚芬,兰干球,蒋钦杨.2014.广西巴马小型猪MCP-1基因克隆及序列分析[J].南方农业学报,45(9):1669-1673.[Song S R,Wu Y J,Jiang S Q,Zhang M Y,Guo Y F,Lan G Q,Jiang Q Y.2014.Cloning and analysis of MCP-1 gene in Guangxi Bama mini-pig[J].Journal of Southern Agriculture,45(9):1669-1673.]
汤磊乐,黄卓山,郑坚锐,陈璘.2017.载脂蛋白A1和载脂蛋白B100在冠脉病变进展中的作用[J].中山大学学报(医学科学版),38(3):395-400.[Tang L L,Huang Z S,Zheng J R,Chen L.2017.Effects of APOA1 and APOB100 on progression of coronary artery lesion[J].Journal of Sun Yat-sen University(Medical Sciences),38(3):395-400.]
田迪.2013.辛伐他汀联合载脂蛋白A-I模拟肽L-4F对apoE基因缺失小鼠高密度脂蛋白抗炎抗氧化功能和动脉粥样硬化的影响[D].广州:南方医科大学.[Tian D.2013.Simvastatin combination with ApoA-I mimetic peptide L-4F improve the anti-oxidant and anti-inflammatory properties of high density lipoprotein and reduce atherosclerosis in apoE knockout mice[D].Guangzhou:Southern Medical University.]
万洁.2008.兔Apo-A1基因多态性及其与部分生产性状的关联研究[D].雅安:四川农业大学.[Wan J.2008.Correlation analysis of production trait and the polymorphism of Apo-A1 gene in rabbits[D].Ya’an:Sichuan Agricultural University.]
王京兰.2004.磷酸化蛋白质分析方法研究及其在人胎肝磷酸化蛋白质组研究中的应用[D].北京:中国人民解放军军事医学科学院.[Wang J L.2004.Analysis of phosphorylated protein and its application in human fetal heparin[D].Beijing:Academy of Military Medical Sciences.]
吴丹,兰干球,郭亚芬,陈宝剑,陈少梅,蒋钦杨.2013.广西巴马小型猪α干扰素基因克隆及其真核表达载体的构建[J].南方农业学报,44(9):1558-1563.[Wu D,Lan GQ,Guo Y F,Chen B J,Chen S M,Jiang Q Y.2013.Cloning interferon-αgene of Guangxi Bama mini-pig and constructing its eukaryotic expression vector[J].Journal of Southern Agriculture,44(9):1558-1563.]
熊婷.2012.鸡ApoA-1和ApoA-V基因多态性与胆固醇含量及繁殖性状的关联研究[D].雅安:四川农业大学.[Xiong T.2012.Polymorphisms of chicken ApoA-1 and ApoA-Vgene and its association with cholesterol content and reproductive traits[D].Ya’an:Sichuan Agricultural University.]
张广杰,崔悦悦,邱庆庆,夏琴,李龙,司景磊,夏攀杰,邹辉,兰干球.2018.广西巴马小型猪SP1基因克隆测序及其真核表达载体的构建[J].南方农业学报,49(2):360-366.[Zhang G J,Cui Y Y,Qiu Q Q,Xia Q,Li L,Si J L,Xia P J,Zou H,Lan G Q.2018.Cloning sequencing of gene SP1 in Guangxi Bama mini pig and construction of its eukaryotic expression vector[J].Journal of Southern A-griculture,49(2):360-366.]
张晶.2012.猪脂肪沉积关键基因的初步筛选[D].郑州:河南农业大学.[Zhang J.2012.Preliminary screening of the key genes of pig fat depositio[D].Zhengzhou:Henan A-gricultural University.]
张旭,高昆,张海涛,高翔,葛文平,张小娟,张连峰,董伟.2011.人载脂蛋白A1基因转基因小鼠的建立[J].中国实验动物学报,19(6):461-464.[Zhang X,Gao K,Zhang H T,Gao X,Ge W P,Zhang X J,Zhang L F,Dong W.2011.Establishment of a human ApoA1 transgenic mouse model[J].Acta Laboratorium Animalis Scientia Sinica,19(6):461-464.]
赵佳福,段志强,杨远青,嵇辛勤,王圆圆,孙成娟.2017.从江香猪ApoA1基因的克隆及亚细胞定位研究[J].中国畜牧兽医,44(7):1954-1960.[Zhao J F,Duan Z Q,Yang YQ,Ji X Q,Wang Y Y,Sun C J.2017.Cloning and subcellular localization of ApoA1 gene in Congjiang Xiang pig[J].Chinese Animal Husbandry&Veterinary Medicine,44(7):1954-1960.]
赵佳福,杨远青,段志强,阮涌,倪萌萌,嵇辛勤.2018.从江香猪ApoA1、ApoC3、ApoE基因在组织器官中表达差异分析[J].黑龙江畜牧兽医,(3):132-136.[Zhao J F,Yang YQ,Duan Z Q,Ruan Y,Ni M M,Ji X Q.2018.The analysis on the different expressions of ApoA1,ApoC3,ApoEgenes in tissues and organs of Congjiang xiang pig[J].Heilongjiang Animal Science and Veterinary Medicine,(3):1954-1960.]
Alonzi T,Mancone C,Amicone L,Tripodi M.2008.Elucidation of lipoprotein particles structure by proteomic analysis[J].Expert Review of Proteomics,5(1):91-104.
Berisha S Z,Brubaker G,Kasumov T,Hung K T,Dibello PM,Huang Y,Li L,Willard B,Pollard K A,Nagy L E,Hazen S L,Smith J D.2015.HDL from apoA1 transgenic mice expressing the 4WF isoform is resistant to oxidative loss of function[J].Journal of Lipid Research,56(3):653-664.
Boettcher A,Loving C,Cunnick J,Tuggle C K.2018.Development of severe combined immunodeficient(SCID)pig models for translational cancer modeling:Future insights on how humanized SCID pigs can improve preclinical cancer research[J].Frontiers in Oncology,8:559.doi:10.3389/fonc.2018.00559.
Chistiakov D A,Orekhov A N,Bobryshev Y V.2016.ApoA1and ApoA1-specific self-antibodies in cardiovascular disease[J].Laboratory Investigation,96(7):708-718.
Hai T,Guo W,Yao J,Cao C,Luo A,Qi M,Wang X,Wang X,Huang J,Zhang Y,Zhang H,Wang D,Shang H,Hong Q,Zhang R,Jia Q,Zheng Q,Qin G,Li Y,Zhang T,Jin W,Chen Z Y,Wang H,Zhou Q,Meng A,Wei H,Yang S,Zhao J.2017.Creation of miniature pig model of human Waardenburg syndrome type 2A by ENU mutagenesis[J].Human Genetics,136(11-12):1463-1475.
Heinecke J W.2013.HDL’s protein cargo:Friend or foe in cardioprotection?[J].Circulation,127(8):868-869.
Hoofnagle A N,Heinecke J W.2009.Lipoproteomics:Using mass spectrometry-based proteomics to explore the assembly,structure,and function of lipoproteins[J].Journal of Lipid Research,50(10):1967-1975.
Ingelsson E,Schaefer E J,Contois J H,Mcnamara J R,Sullivan L,Keyes M J,Pencina M J,Schoonmaker C,Wilson P W,D'Agostino R B,Vasan R S.2007.Clinical utility of different lipid measures for prediction of coronary heart disease in men and women[J].The Journal of the American Medical Association,298(7):776-785.
Li Y,Cheng Y W,Zhu T Y,Zhang H,Li W,Guo Y S,Qi YL,Chen X,Zhang J,Sha J H,Zhou Z M,Zhu H,Guo XJ.2019.The protein phosphorylation landscape of mouse spermatids during spermiogenesis[J].Proteomics,19(11).doi:10.1002/pmic.201900055.
Luo F,Wang M,Liu Y,Zhao X M,Li A.2019.DeepPhos:Prediction of protein phosphorylation sites with deep learning[J].Bioinformatics.doi:10.1093/bioinformatics/bty1051.
Rader D J.2006.Molecular regulation of HDL metabolism and function:Implications for novel therapies[J].The Journal of Clinical Investigation,116(12):3090-3100.
Wang X P,He J,Guo H,Mu L,Hu Y H,Ma J L,Yan Y Z,Ma R L,Li S G,Ding Y S,Zhang M,Niu Q,Liu J M,Zhang J Y,Guo S X.2017.Interactions of six SNPs in APOA1 gene and types of obesity on low HDL-C disease in Xinjiang pastoral area of China[J].Lipids in Health and Disease,16:187.
Wu Y J,Zhang L,Liang J,Jiang Q Y,Guo Y F,Lan G Q.2018.Comparative analysis on liver transcriptome profiles of different methods to establish type 2 diabetes mellitus models in Guangxi Bama mini-pig[J].Gene,673:194-200.
Yan X Y,Wu Y J,Zhong F J,Jiang Q Y,Zhou T T,Guo Y F,Yang X R,Liang J,Liao J D,Lan G Q.2018.iTRAQand PRM-based quantitative proteomics in T2DM-susceptible and-tolerant models of Bama mini-pig[J].Gene,675:119-127.
Zhang Y,Zhang L,Wang Y,Ding H,Xue S,Yu H,Hu L,Qi H,Wang Y,Zhu W,Liu D,Li P.2019.KCNQ1OT1,HIF1A-AS2 and APOA1-AS are promising novel biomarkers for diagnosis of coronary artery disease[J].Clinical and Experimental Pharmacology and Physiology,46(7):635-642.
阚延泽,江翱,孙文秀,李伟.2016.黄鳝载脂蛋白A1的基因结构及表达谱分析[J].水产科学,35(6):697-701.[Kan Y Z,Jiang A,Sun W X,Li W.2016.Gene structure and expression of ApoA1 gene from swamp eel[J].Fisheries Science,35(6):697-701.]
雷蕾,陈丽.2011.血浆载脂蛋白与冠心病的相关性研究[J].当代医学,17(12):13-14.[Lei L,Chen L.2011.Study on the correlation between plasma apolipoprotein and coronary heart disease[J].Contemporary Medicine,17(12):13-14.]
李芳芳,郭亚芬,兰干球,潘堂峰.2014.广西巴马小型猪GHRH成熟肽真核表达质粒对小鼠生长效应的影响[J].南方农业学报,45(12):2264-2269.[Li F F,Guo Y F,Lan G Q,Pan T F.2014.Eukaryotic expression plasmid of GHRH mature peptide from Guangxi Bama Mini-pig and its influence on mouse growth effect[J].Journal of Southern Agriculture,45(12):2264-2269.
李龙,司景磊,夏攀洁,何剑雄,吴延军,程晓芳,吴敏,徐文文,兰干球.2017.广西巴马小型猪Neuritin基因克隆与生物信息学分析及真核表达载体的构建[J].基因组学与应用生物学,36(2):557-563.[Li L,Si J L,Xia P J,He J X,Wu Y J,Cheng X F,Wu M,Xu W W,Lan GQ.2017.Cloning and bioinformatics analysis of Neuritin gene from Guangxi Bama Mini-pig and construction of its eukaryotic expression vector[J].Genomics and Applied Biology,36(2):557-563.]
刘培彬,叶子璐,蔡潭溪,戴绍军,杨福全.2014.脂蛋白质组学研究进展[J].生物化学与生物物理进展,41(12):1197-1206.[Liu P B,Ye Z L,Cai T X,Dai S J,Yang F Q.2014.The research advances in lipoproteinomics[J].Progress in Biochemistry and Biophysics,41(12):1197-1206.]
麻延峰,方倩倩,王宏艳,田勇,唐军旺,邵荣益,卢立志.2015.鹅ApoA1、CD36和DGAT2基因的cDNA克隆与序列分析[J].浙江农业学报,27(10):1710-1719.[Ma Y F,Fang Q Q,Wang H Y,Tian Y,Tang J W,Shao R Y,Lu L Z.2015.cDNA cloning and sequence analysis of ApoA1,CD36 and DGAT2 genes from goose[J].Acta Agriculturae Zhejiangensis,27(10):1710-1719.]
申玉建,方程,邱庆庆,司景磊,吴延军,杨祝良,杨秀荣,郭亚芬,蒋和生.2017.广西巴马小型猪PPARγ基因克隆及组织表达差异分析[J].南方农业学报,48(10):1884-1890.[Shen Y J,Fang C,Qiu Q Q,Si J L,Wu Y J,Yang Z L,Yang X R,Guo Y F,Jiang H S.2017.Cloning of gene PPARγfrom Guangxi Bama mini-pig and tissue expression difference analysis for it[J].Journal of Southern Agriculture,48(10):1884-1890.]
宋少锐,吴延军,蒋世强,张名媛,郭亚芬,兰干球,蒋钦杨.2014.广西巴马小型猪MCP-1基因克隆及序列分析[J].南方农业学报,45(9):1669-1673.[Song S R,Wu Y J,Jiang S Q,Zhang M Y,Guo Y F,Lan G Q,Jiang Q Y.2014.Cloning and analysis of MCP-1 gene in Guangxi Bama mini-pig[J].Journal of Southern Agriculture,45(9):1669-1673.]
汤磊乐,黄卓山,郑坚锐,陈璘.2017.载脂蛋白A1和载脂蛋白B100在冠脉病变进展中的作用[J].中山大学学报(医学科学版),38(3):395-400.[Tang L L,Huang Z S,Zheng J R,Chen L.2017.Effects of APOA1 and APOB100 on progression of coronary artery lesion[J].Journal of Sun Yat-sen University(Medical Sciences),38(3):395-400.]
田迪.2013.辛伐他汀联合载脂蛋白A-I模拟肽L-4F对apoE基因缺失小鼠高密度脂蛋白抗炎抗氧化功能和动脉粥样硬化的影响[D].广州:南方医科大学.[Tian D.2013.Simvastatin combination with ApoA-I mimetic peptide L-4F improve the anti-oxidant and anti-inflammatory properties of high density lipoprotein and reduce atherosclerosis in apoE knockout mice[D].Guangzhou:Southern Medical University.]
万洁.2008.兔Apo-A1基因多态性及其与部分生产性状的关联研究[D].雅安:四川农业大学.[Wan J.2008.Correlation analysis of production trait and the polymorphism of Apo-A1 gene in rabbits[D].Ya’an:Sichuan Agricultural University.]
王京兰.2004.磷酸化蛋白质分析方法研究及其在人胎肝磷酸化蛋白质组研究中的应用[D].北京:中国人民解放军军事医学科学院.[Wang J L.2004.Analysis of phosphorylated protein and its application in human fetal heparin[D].Beijing:Academy of Military Medical Sciences.]
吴丹,兰干球,郭亚芬,陈宝剑,陈少梅,蒋钦杨.2013.广西巴马小型猪α干扰素基因克隆及其真核表达载体的构建[J].南方农业学报,44(9):1558-1563.[Wu D,Lan GQ,Guo Y F,Chen B J,Chen S M,Jiang Q Y.2013.Cloning interferon-αgene of Guangxi Bama mini-pig and constructing its eukaryotic expression vector[J].Journal of Southern Agriculture,44(9):1558-1563.]
熊婷.2012.鸡ApoA-1和ApoA-V基因多态性与胆固醇含量及繁殖性状的关联研究[D].雅安:四川农业大学.[Xiong T.2012.Polymorphisms of chicken ApoA-1 and ApoA-Vgene and its association with cholesterol content and reproductive traits[D].Ya’an:Sichuan Agricultural University.]
张广杰,崔悦悦,邱庆庆,夏琴,李龙,司景磊,夏攀杰,邹辉,兰干球.2018.广西巴马小型猪SP1基因克隆测序及其真核表达载体的构建[J].南方农业学报,49(2):360-366.[Zhang G J,Cui Y Y,Qiu Q Q,Xia Q,Li L,Si J L,Xia P J,Zou H,Lan G Q.2018.Cloning sequencing of gene SP1 in Guangxi Bama mini pig and construction of its eukaryotic expression vector[J].Journal of Southern A-griculture,49(2):360-366.]
张晶.2012.猪脂肪沉积关键基因的初步筛选[D].郑州:河南农业大学.[Zhang J.2012.Preliminary screening of the key genes of pig fat depositio[D].Zhengzhou:Henan A-gricultural University.]
张旭,高昆,张海涛,高翔,葛文平,张小娟,张连峰,董伟.2011.人载脂蛋白A1基因转基因小鼠的建立[J].中国实验动物学报,19(6):461-464.[Zhang X,Gao K,Zhang H T,Gao X,Ge W P,Zhang X J,Zhang L F,Dong W.2011.Establishment of a human ApoA1 transgenic mouse model[J].Acta Laboratorium Animalis Scientia Sinica,19(6):461-464.]
赵佳福,段志强,杨远青,嵇辛勤,王圆圆,孙成娟.2017.从江香猪ApoA1基因的克隆及亚细胞定位研究[J].中国畜牧兽医,44(7):1954-1960.[Zhao J F,Duan Z Q,Yang YQ,Ji X Q,Wang Y Y,Sun C J.2017.Cloning and subcellular localization of ApoA1 gene in Congjiang Xiang pig[J].Chinese Animal Husbandry&Veterinary Medicine,44(7):1954-1960.]
赵佳福,杨远青,段志强,阮涌,倪萌萌,嵇辛勤.2018.从江香猪ApoA1、ApoC3、ApoE基因在组织器官中表达差异分析[J].黑龙江畜牧兽医,(3):132-136.[Zhao J F,Yang YQ,Duan Z Q,Ruan Y,Ni M M,Ji X Q.2018.The analysis on the different expressions of ApoA1,ApoC3,ApoEgenes in tissues and organs of Congjiang xiang pig[J].Heilongjiang Animal Science and Veterinary Medicine,(3):1954-1960.]
Alonzi T,Mancone C,Amicone L,Tripodi M.2008.Elucidation of lipoprotein particles structure by proteomic analysis[J].Expert Review of Proteomics,5(1):91-104.
Berisha S Z,Brubaker G,Kasumov T,Hung K T,Dibello PM,Huang Y,Li L,Willard B,Pollard K A,Nagy L E,Hazen S L,Smith J D.2015.HDL from apoA1 transgenic mice expressing the 4WF isoform is resistant to oxidative loss of function[J].Journal of Lipid Research,56(3):653-664.
Boettcher A,Loving C,Cunnick J,Tuggle C K.2018.Development of severe combined immunodeficient(SCID)pig models for translational cancer modeling:Future insights on how humanized SCID pigs can improve preclinical cancer research[J].Frontiers in Oncology,8:559.doi:10.3389/fonc.2018.00559.
Chistiakov D A,Orekhov A N,Bobryshev Y V.2016.ApoA1and ApoA1-specific self-antibodies in cardiovascular disease[J].Laboratory Investigation,96(7):708-718.
Hai T,Guo W,Yao J,Cao C,Luo A,Qi M,Wang X,Wang X,Huang J,Zhang Y,Zhang H,Wang D,Shang H,Hong Q,Zhang R,Jia Q,Zheng Q,Qin G,Li Y,Zhang T,Jin W,Chen Z Y,Wang H,Zhou Q,Meng A,Wei H,Yang S,Zhao J.2017.Creation of miniature pig model of human Waardenburg syndrome type 2A by ENU mutagenesis[J].Human Genetics,136(11-12):1463-1475.
Heinecke J W.2013.HDL’s protein cargo:Friend or foe in cardioprotection?[J].Circulation,127(8):868-869.
Hoofnagle A N,Heinecke J W.2009.Lipoproteomics:Using mass spectrometry-based proteomics to explore the assembly,structure,and function of lipoproteins[J].Journal of Lipid Research,50(10):1967-1975.
Ingelsson E,Schaefer E J,Contois J H,Mcnamara J R,Sullivan L,Keyes M J,Pencina M J,Schoonmaker C,Wilson P W,D'Agostino R B,Vasan R S.2007.Clinical utility of different lipid measures for prediction of coronary heart disease in men and women[J].The Journal of the American Medical Association,298(7):776-785.
Li Y,Cheng Y W,Zhu T Y,Zhang H,Li W,Guo Y S,Qi YL,Chen X,Zhang J,Sha J H,Zhou Z M,Zhu H,Guo XJ.2019.The protein phosphorylation landscape of mouse spermatids during spermiogenesis[J].Proteomics,19(11).doi:10.1002/pmic.201900055.
Luo F,Wang M,Liu Y,Zhao X M,Li A.2019.DeepPhos:Prediction of protein phosphorylation sites with deep learning[J].Bioinformatics.doi:10.1093/bioinformatics/bty1051.
Rader D J.2006.Molecular regulation of HDL metabolism and function:Implications for novel therapies[J].The Journal of Clinical Investigation,116(12):3090-3100.
Wang X P,He J,Guo H,Mu L,Hu Y H,Ma J L,Yan Y Z,Ma R L,Li S G,Ding Y S,Zhang M,Niu Q,Liu J M,Zhang J Y,Guo S X.2017.Interactions of six SNPs in APOA1 gene and types of obesity on low HDL-C disease in Xinjiang pastoral area of China[J].Lipids in Health and Disease,16:187.
Wu Y J,Zhang L,Liang J,Jiang Q Y,Guo Y F,Lan G Q.2018.Comparative analysis on liver transcriptome profiles of different methods to establish type 2 diabetes mellitus models in Guangxi Bama mini-pig[J].Gene,673:194-200.
Yan X Y,Wu Y J,Zhong F J,Jiang Q Y,Zhou T T,Guo Y F,Yang X R,Liang J,Liao J D,Lan G Q.2018.iTRAQand PRM-based quantitative proteomics in T2DM-susceptible and-tolerant models of Bama mini-pig[J].Gene,675:119-127.
Zhang Y,Zhang L,Wang Y,Ding H,Xue S,Yu H,Hu L,Qi H,Wang Y,Zhu W,Liu D,Li P.2019.KCNQ1OT1,HIF1A-AS2 and APOA1-AS are promising novel biomarkers for diagnosis of coronary artery disease[J].Clinical and Experimental Pharmacology and Physiology,46(7):635-642.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |