不同产地白芷药材9个呋喃香豆素成分的含量测定及其质量评价
|
摘要
建立白芷呋喃香豆素成分定量测定方法,并用于不同产地白芷的质量评价。采用Waters UPLC,ACQUITY UPLC BEH C18色谱柱,流动相(乙腈-水)梯度洗脱,检测波长254 nm,同时测定10批白芷药材中花椒毒酚、水合氧化前胡素、白当归素、补骨脂素、花椒毒素、佛手柑内酯、氧化前胡素、欧前胡素和异欧前胡素的含量;并结合相似度评价、聚类分析(CA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)等化学模式识别技术,对不同产地白芷药材的总体质量进行分析评价。所测呋喃香豆素成分的含量从高到低依次为:氧化前胡素>欧前胡素>异欧前胡素>水和氧化前胡素>佛手柑内酯>白当归素>花椒毒素>花椒毒酚>补骨脂素,均值分别为2. 844,1. 277,0. 649 2,0. 216 2,0. 129 8,0. 062 68,0. 052 68,0. 019 30,0. 018 19 mg·g-1;经含量测定和化学模式识别分析结果可知不同产地白芷药材质量有差异,且硫磺熏蒸对白芷药材品质有较大影响;最后采用OPLS-DA,筛选出了导致各批次白芷药材质量差异的6个主要标志性成分。该实验建立的含量测定结合化学模式识别方法可用于白芷药材的综合质量评价。
A UPLC method has been developed for simultaneous determination of nine furanocoumarins of Angelica dahurics,and was used for quality evaluation of A. dahurica from different habitats. ACQUITY UPLC BEH C18 chromatographic column was employed,the separation was performed with the mobile phase consisting of acetonitrile and water,and the detection wavelength was set at254 nm. This method was used to simultaneously determine the content of xanthotoxol,oxypeucedaninhydrate,byak-angelicin,psoralen,xanthotoxin,bergapten,oxypeucedanin,imperatorin and isoimperatorin in A. dahurica from different habitats. Then,the further quality assessment of the drug was carried out by similarity evaluation,cluster analysis( CA),principal component analysis( PCA),and orthogonal partial least squares discriminant analysis( OPLS-DA). The content order of measured furanocoumarins from high to low was: oxypeucedanin>imperatorin>isoimperatorin>oxypeucedaninhydrate>bergapten>byak-angelicin>xanthotoxin>xanthotoxol>psoralen,with the mean content 2. 844,1. 277,0. 649 2,0. 216 2,0. 129 8,0. 062 68,0. 052 68,0. 019 30,0. 018 19 mg·g-1,respectively. There were difference between the batches of the drug,and the quality was influenced by smouldering sulphur based on the results of chemical pattern recognition and content determination. Finally,six active ingredients were recognized as the quality makers using OPLS-DA method. The validated UPLC fingerprint combined with chemical pattern recognition method can be used in the quality control and evaluation of A. dahurica.
A UPLC method has been developed for simultaneous determination of nine furanocoumarins of Angelica dahurics,and was used for quality evaluation of A. dahurica from different habitats. ACQUITY UPLC BEH C18 chromatographic column was employed,the separation was performed with the mobile phase consisting of acetonitrile and water,and the detection wavelength was set at254 nm. This method was used to simultaneously determine the content of xanthotoxol,oxypeucedaninhydrate,byak-angelicin,psoralen,xanthotoxin,bergapten,oxypeucedanin,imperatorin and isoimperatorin in A. dahurica from different habitats. Then,the further quality assessment of the drug was carried out by similarity evaluation,cluster analysis( CA),principal component analysis( PCA),and orthogonal partial least squares discriminant analysis( OPLS-DA). The content order of measured furanocoumarins from high to low was: oxypeucedanin>imperatorin>isoimperatorin>oxypeucedaninhydrate>bergapten>byak-angelicin>xanthotoxin>xanthotoxol>psoralen,with the mean content 2. 844,1. 277,0. 649 2,0. 216 2,0. 129 8,0. 062 68,0. 052 68,0. 019 30,0. 018 19 mg·g-1,respectively. There were difference between the batches of the drug,and the quality was influenced by smouldering sulphur based on the results of chemical pattern recognition and content determination. Finally,six active ingredients were recognized as the quality makers using OPLS-DA method. The validated UPLC fingerprint combined with chemical pattern recognition method can be used in the quality control and evaluation of A. dahurica.
引文
[1] Chen L,Yang H,Yu C,et al. High hepatic exposure of furanocoumarins in Radix Angelica Dahuricae is associated with transporter mediated active uptake[J]. J Ethnopharmacol,2018,212:74.
[2]史洋,雷云,许海玉,等.白芷中3个主要活性成分含量测定及其质量评价研究[J].中国中药杂志,2015,40(5):915.
[3]蒋运斌,卢晓琳,杨枝中,等.与熏硫加工相关的白芷化学成分研究[J].中国实验方剂学杂志,2013,19(22):74.
[4]马逾英,高颖,邹文莉,等.熏硫川白芷药材对小鼠镇痛作用的影响[J].华西药学杂志,2006,21(6):616.
[5] Choi I H,Song Y,Lim H H. Analgesic and anti-inflammatory effect of the aqueous extract of Angelica dahurica[J]. J. Korean Oriental Med,2008,29(2):32.
[6] Márquez N,Sancho R,Ballero M,et al. Imperatorin inhibits T-cell proliferation by targeting the transcription factor NFAT[J].Planta Med,2004,70:1016.
[7] Thuong P T,Hung T M,Ngoc T M,et al. Antioxidant activities of coumarins from Korean medicinal plants and their structureactivity relationships[J]. Phytother Res,2010,24(1):101.
[8] Luo K W,Sun J G,Chan J Y,et al. Anticancer effects of imperatorin isolated from Angelica dahurica:induction of apoptosis in HepG2 cells through both death-receptor-and mitochondria-mediated pathways[J]. Chemotherapy,2010,57(6):449.
[9] Cao Y J,He X,Wang N,et al. Effects of imperatorin,the active component from Radix Angelicae(Baizhi),on the blood pressure and oxidative stress in 2K,1C hypertensive rats[J]. Phytomedicine,2013,20(12):1048.
[10] Chen L,Jian Y,Wei N,et al. Separation and simultaneous quantification of nine furanocoumarins from Radix Angelicae Dahuricae using liquid chromatography with tandem mass spectrometry for bioavailability determination in rats[J]. J Sep Sci,2015,38(24):4216.
[11] Huo H L,Yu S H,Liu X Z,et al. Simultaneous and sensitive determination of eight coumarins in rat bile and urine after oral administration of Radix Angelicae Dahuricae extract by liquid chromatography-electrospray ionization-mass spectrometry[J].Acta Chromatogr,2013,25:201.
[12] Liang B,Xu L Z,Zou Z M,et al. Chemical constituents isolated from Angelica dahurica var. formosana[J]. Chin Tradit Herbal Drugs,2005,36(8):1132.
[13]中国药典[S].一部. 2015.
[14] Li B,Zhang X,Wang J,et al. Simultaneous characterisation of fifty coumarins from the roots of Angelica dahurica by off-line twodimensional high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry[J]. Phytochem Anal,2014,25(3):229.
[15] Pfeifer I,Murauer A,Ganzera M. Determination of coumarins in the roots of Angelica dahurica by supercritical fluid chromatography[J]. J Pharm Biomed Anal,2016,129:246.
[16]雷云,王威,史洋,等.基于化学指纹图谱和多指标成分含量测定的白芷质量评价[J].中国实验方剂学杂志,2015,21(2):36.
[17]康建,周霖,孙志,等.基于UPLC指纹图谱结合化学模式识别的冠心丹参胶囊质量控制研究[J].中草药,2018,49(17):4051.
[2]史洋,雷云,许海玉,等.白芷中3个主要活性成分含量测定及其质量评价研究[J].中国中药杂志,2015,40(5):915.
[3]蒋运斌,卢晓琳,杨枝中,等.与熏硫加工相关的白芷化学成分研究[J].中国实验方剂学杂志,2013,19(22):74.
[4]马逾英,高颖,邹文莉,等.熏硫川白芷药材对小鼠镇痛作用的影响[J].华西药学杂志,2006,21(6):616.
[5] Choi I H,Song Y,Lim H H. Analgesic and anti-inflammatory effect of the aqueous extract of Angelica dahurica[J]. J. Korean Oriental Med,2008,29(2):32.
[6] Márquez N,Sancho R,Ballero M,et al. Imperatorin inhibits T-cell proliferation by targeting the transcription factor NFAT[J].Planta Med,2004,70:1016.
[7] Thuong P T,Hung T M,Ngoc T M,et al. Antioxidant activities of coumarins from Korean medicinal plants and their structureactivity relationships[J]. Phytother Res,2010,24(1):101.
[8] Luo K W,Sun J G,Chan J Y,et al. Anticancer effects of imperatorin isolated from Angelica dahurica:induction of apoptosis in HepG2 cells through both death-receptor-and mitochondria-mediated pathways[J]. Chemotherapy,2010,57(6):449.
[9] Cao Y J,He X,Wang N,et al. Effects of imperatorin,the active component from Radix Angelicae(Baizhi),on the blood pressure and oxidative stress in 2K,1C hypertensive rats[J]. Phytomedicine,2013,20(12):1048.
[10] Chen L,Jian Y,Wei N,et al. Separation and simultaneous quantification of nine furanocoumarins from Radix Angelicae Dahuricae using liquid chromatography with tandem mass spectrometry for bioavailability determination in rats[J]. J Sep Sci,2015,38(24):4216.
[11] Huo H L,Yu S H,Liu X Z,et al. Simultaneous and sensitive determination of eight coumarins in rat bile and urine after oral administration of Radix Angelicae Dahuricae extract by liquid chromatography-electrospray ionization-mass spectrometry[J].Acta Chromatogr,2013,25:201.
[12] Liang B,Xu L Z,Zou Z M,et al. Chemical constituents isolated from Angelica dahurica var. formosana[J]. Chin Tradit Herbal Drugs,2005,36(8):1132.
[13]中国药典[S].一部. 2015.
[14] Li B,Zhang X,Wang J,et al. Simultaneous characterisation of fifty coumarins from the roots of Angelica dahurica by off-line twodimensional high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry[J]. Phytochem Anal,2014,25(3):229.
[15] Pfeifer I,Murauer A,Ganzera M. Determination of coumarins in the roots of Angelica dahurica by supercritical fluid chromatography[J]. J Pharm Biomed Anal,2016,129:246.
[16]雷云,王威,史洋,等.基于化学指纹图谱和多指标成分含量测定的白芷质量评价[J].中国实验方剂学杂志,2015,21(2):36.
[17]康建,周霖,孙志,等.基于UPLC指纹图谱结合化学模式识别的冠心丹参胶囊质量控制研究[J].中草药,2018,49(17):4051.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |