代谢综合征合并阻塞性睡眠呼吸暂停低通气综合征患者CDKN2A/2B基因多态性的研究
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摘要
目的探讨细胞周期蛋白依赖激酶抑制剂(cyclin-dependent kinase inhibitor 2A/2B,CDKN2A/2B)基因多态性与代谢综合征合并阻塞性睡眠呼吸暂停低通气综合征患者的关系。方法选取2017年6月—2018年11月我院门诊就诊的216例代谢综合征患者,根据多导睡眠仪诊断为阻塞性睡眠呼吸暂停低通气综合征患者116例为观察组,单纯代谢综合征患者100例为代谢综合征对照组,选取同期我院健康查体者98例为健康对照组。分别采用聚合酶链反应限制性片段长度多态性测定三组患者CDKN2A/2B基因rs10811661基因型分布及频率。结果三组患者CDKN2A/2B基因rs10811661的TT、TC、CC三种基因型频率分布比较:与健康对照组比较,代谢综合征对照组和观察组三种基因型频率分布差异均有统计学意义(P<0.05),与代谢综合征对照组比较,观察组三种基因型频率分布差异无统计学意义(P>0.05)。三组患者CDKN2A/2B基因rs10811661的T、C两种等位基因频率分布比较:与健康对照组比较,代谢综合征对照组和观察组T、C两种等位基因频率分布差异均有统计学意义(P<0.05),其中观察组等位基因T分布频率明显高于健康对照组(P<0.05),与代谢综合征对照组比较,观察组等位基因型频率分布差异无统计学意义(P>0.05)。结论 CDKN2A/2B基因rs10811661多态性可能与代谢综合征合并阻塞性睡眠呼吸暂停低通气综合征的发病有关,存在一定的易感性。
Objective To investigate the relationship between CDKN2 A/2 B gene polymorphism and patients with metabolic syndrome and obstructive sleep apnea hypopnea syndrome.Methods From January 2016 to December 2017,two hundred and sixteen patients with metabolic syndrome in Affiliated Hospital of North China University of Science and Technology were selected.116 patients who were diagnosed with obstructive sleep apnea-hypopnea syndrome according to polysomnography were selected as observation group,100 patients with pure metabolic syndrome were selected as control group of metabolic syndrome,and 98 cases of health examination during the same period in our hospital were selected as healthy control group.The distribution and frequency of CDKN2 A/2 B gene rs10811661 genotype were determined by polymerase chain reaction restriction fragment length polymorphism.Results The distribution of TT,TC and CC genotypes of CDKN2 A/2 B gene rs10811661 was significantly different between control group and control group of metabolic syndrome(P<0.05).The frequency of T allele distribution in the control group of metabolic syndrome was significantly higher than that in the control group(P<0.05).The distribution of the three genotypes in the observation group was significantly higher than that in the control group(P<0.05).The frequency of T allele distribution in observation group was significantly higher than that of control group(P<0.05).There was no significant difference in genotype and allele frequency distribution between the two groups(P>0.05).Conclusion The rs10811661 polymorphism of CDKN2 A/2 B gene may be associated with the development ofmetabolic syndrome with obstructive sleep apnea hypopnea syndrome and have a certain susceptibility.
Objective To investigate the relationship between CDKN2 A/2 B gene polymorphism and patients with metabolic syndrome and obstructive sleep apnea hypopnea syndrome.Methods From January 2016 to December 2017,two hundred and sixteen patients with metabolic syndrome in Affiliated Hospital of North China University of Science and Technology were selected.116 patients who were diagnosed with obstructive sleep apnea-hypopnea syndrome according to polysomnography were selected as observation group,100 patients with pure metabolic syndrome were selected as control group of metabolic syndrome,and 98 cases of health examination during the same period in our hospital were selected as healthy control group.The distribution and frequency of CDKN2 A/2 B gene rs10811661 genotype were determined by polymerase chain reaction restriction fragment length polymorphism.Results The distribution of TT,TC and CC genotypes of CDKN2 A/2 B gene rs10811661 was significantly different between control group and control group of metabolic syndrome(P<0.05).The frequency of T allele distribution in the control group of metabolic syndrome was significantly higher than that in the control group(P<0.05).The distribution of the three genotypes in the observation group was significantly higher than that in the control group(P<0.05).The frequency of T allele distribution in observation group was significantly higher than that of control group(P<0.05).There was no significant difference in genotype and allele frequency distribution between the two groups(P>0.05).Conclusion The rs10811661 polymorphism of CDKN2 A/2 B gene may be associated with the development ofmetabolic syndrome with obstructive sleep apnea hypopnea syndrome and have a certain susceptibility.
引文
[1] 许梅杰,张希龙.阻塞性睡眠呼吸暂停与代谢综合征[J].临床内科杂志,2017,34(1):19-21
[2] 李文鹏.阻塞性睡眠呼吸暂停综合征与代谢综合征[J].中国循环杂志,2015,30(5):511-512
[3] Bozkurt NC,Beysel S,Karbek B,et al.Visceral Obesity Mediates the Association Between Metabolic Syndrome and Obstructive SleepApnea Syndrome[J].Metab Syndr Relat Disord,2016,14(4):217-221
[4] 李梅,姚晓光,李秀芳,等.Glut4基因和IL-1β基因多态性和阻塞性睡眠呼吸暂停综合征危险因素间的交互作用[J].中华生物医学工程杂志,2018,24(1):43-48
[5] 李奕平,杨莹,鲁帅尧,等.CDH13基因多态性与中国人群代谢综合征的相关性[J].中华医学遗传学杂志,2018,35(1):107-111
[6] 刘杰,王淑贞,王巧莲,等.妊娠糖尿病患者血糖、血脂水平与CDKN2A/2B基因多态性的相关性[J].标记免疫分析与临床,2018,25(9):1360-1363
[7] 中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013年版)[J].中国糖尿病杂志,2014,22 (8):2-42
[8] 中华医学会呼吸病学分会睡眠呼吸障碍学组.阻塞性睡眠呼吸暂停低通气综合征诊治指南(2011年修订版)[J].中华结核和呼吸杂志,2012,35(1):9-12
[9] 郭建梅,赵季红.慢性心力衰竭合并阻塞性睡眠呼吸暂停低通气综合征患者经鼻持续气道正压通气治疗效果分析[J].中国医药,2019,14(2):204-207
[10] 胡碧,王茂筠,王怡唯,等.夜间动态血氧饱和度监测对代谢综合征合并阻塞性睡眠呼吸暂停低通气综合征患者的意义研究[J].中国全科医学,2017,20(11):1287-1293
[11] 华娜,李宪华,姜涛,等.手术对重度阻塞性睡眠呼吸暂停低通气综合征合并代谢综合征患者疗效分析[J].中国耳鼻咽喉头颈外科,2018,25(5):259-261
[12] 吴娴.代谢综合征相关基因多态性研究进展[J].国际生殖健康/计划生育杂志,2015,34(2):165-168
[13] 张冬梅,肖毅.阻塞性睡眠呼吸暂停低通气综合征基因研究进展[J].中华结核和呼吸杂志,2014,37(11):855-857
[14] 高碧珍,陈继承,林小凤,等.代谢综合征“痰证”人群与CDKALI、CDKN2A/2B基因的关系[J].中华中医药杂志,2016,31(11):4446-4449
[15] 陈琰,赵莹,刘桂锋.SLC30A8、CDKN2A/2B、HHE- X、TCF7L2基因多态性与中国东北汉族人2型糖尿病的关联[J].中国老年学杂志,2015,35(15):4201-4204
[16] 梅劫,王海莲,刘运强,等.CDKN2A/2B的单核苷酸多态性与妊娠妇女糖耐量异常的相关性分析[J].中华内分泌外科杂,2014,8(3):189-192
[2] 李文鹏.阻塞性睡眠呼吸暂停综合征与代谢综合征[J].中国循环杂志,2015,30(5):511-512
[3] Bozkurt NC,Beysel S,Karbek B,et al.Visceral Obesity Mediates the Association Between Metabolic Syndrome and Obstructive SleepApnea Syndrome[J].Metab Syndr Relat Disord,2016,14(4):217-221
[4] 李梅,姚晓光,李秀芳,等.Glut4基因和IL-1β基因多态性和阻塞性睡眠呼吸暂停综合征危险因素间的交互作用[J].中华生物医学工程杂志,2018,24(1):43-48
[5] 李奕平,杨莹,鲁帅尧,等.CDH13基因多态性与中国人群代谢综合征的相关性[J].中华医学遗传学杂志,2018,35(1):107-111
[6] 刘杰,王淑贞,王巧莲,等.妊娠糖尿病患者血糖、血脂水平与CDKN2A/2B基因多态性的相关性[J].标记免疫分析与临床,2018,25(9):1360-1363
[7] 中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013年版)[J].中国糖尿病杂志,2014,22 (8):2-42
[8] 中华医学会呼吸病学分会睡眠呼吸障碍学组.阻塞性睡眠呼吸暂停低通气综合征诊治指南(2011年修订版)[J].中华结核和呼吸杂志,2012,35(1):9-12
[9] 郭建梅,赵季红.慢性心力衰竭合并阻塞性睡眠呼吸暂停低通气综合征患者经鼻持续气道正压通气治疗效果分析[J].中国医药,2019,14(2):204-207
[10] 胡碧,王茂筠,王怡唯,等.夜间动态血氧饱和度监测对代谢综合征合并阻塞性睡眠呼吸暂停低通气综合征患者的意义研究[J].中国全科医学,2017,20(11):1287-1293
[11] 华娜,李宪华,姜涛,等.手术对重度阻塞性睡眠呼吸暂停低通气综合征合并代谢综合征患者疗效分析[J].中国耳鼻咽喉头颈外科,2018,25(5):259-261
[12] 吴娴.代谢综合征相关基因多态性研究进展[J].国际生殖健康/计划生育杂志,2015,34(2):165-168
[13] 张冬梅,肖毅.阻塞性睡眠呼吸暂停低通气综合征基因研究进展[J].中华结核和呼吸杂志,2014,37(11):855-857
[14] 高碧珍,陈继承,林小凤,等.代谢综合征“痰证”人群与CDKALI、CDKN2A/2B基因的关系[J].中华中医药杂志,2016,31(11):4446-4449
[15] 陈琰,赵莹,刘桂锋.SLC30A8、CDKN2A/2B、HHE- X、TCF7L2基因多态性与中国东北汉族人2型糖尿病的关联[J].中国老年学杂志,2015,35(15):4201-4204
[16] 梅劫,王海莲,刘运强,等.CDKN2A/2B的单核苷酸多态性与妊娠妇女糖耐量异常的相关性分析[J].中华内分泌外科杂,2014,8(3):189-192
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