NF-KB p65蛋白在皮肤鳞状细胞癌中的表达及临床意义
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摘要
目的探讨NF-KB p65蛋白在皮肤鳞状细胞癌中的表达及临床意义。方法选自我院于2015年1月至2018年1月期间皮肤鳞状细胞癌行手术切除的皮肤鳞状细胞癌组织标本53例作为研究对象(研究组);另选自我院于2015年1月至2018年1月正常皮肤组织30例作为对照组。采用免疫组化法检测NF-kB p65蛋白表达。比较两组NF-kB p65蛋白阳性表达情况,不同性别、不同年龄、不同临床分期及不同分化程度NF-kBp65蛋白阳性表达情况。结果研究组NF-kBp65蛋白阳性率高于对照组,且有统计学差异(P<0.05);不同性别和不同年龄皮肤鳞状细胞癌NF-kBp65阳性表达率比较无统计学差异(P>0.05);临床分期III～IV期皮肤鳞状细胞癌NF-kBp65阳性表达率高于I～II期,低分化皮肤鳞状细胞癌NF-kBp65阳性表达率高于高、中分化,具有统计学差异(P<0.05)。结论 NF-KBp65蛋白在皮肤鳞状细胞癌中呈高表达,且随着病情加重表达越高,认为可作为判断病情程度和治疗的新靶点,值得临床借鉴。
Objective:To investigate the expression and clinical significance of NF-KB p65 protein in cutaneous squamous cell carcinoma. Methods:53 cases of skin squamous cell carcinoma were selected from January 2015 to January 2018 in our hospital,and 30 cases of normal skin tissues from January 2015 to January 2018 were selected as the control group. Immunohistochemical method was used to detect the expression of NF-kB p65 protein. The positive expression of NF-kB p65 protein in two groups was compared,and the positive expression of NF-kB p65 protein in different sex,different age,different clinical stages and different degree of differentiation. Results:The positive rate of NF-kB p65 protein in the study group was higher than that of the control group(P<0.05). The positive rate of NF-kB p65 in the squamous cell carcinoma of the skin of different sex and different age was not statistically different(P>0.05),and the positive rate of NF-kB p65 positive in III～IV stage squamous cell carcinoma of III～IV stage was higher than that of I～II and low differentiation. The positive expression rate of NF-kB p65 in cutaneous squamous cell carcinoma was higher than that in high and medium differentiation(P<0.05). Conclusion:NF-KB p65 protein is highly expressed in the squamous cell carcinoma of the skin,and the higher the state of the disease is,the higher the expression is,it is considered as a new target for judging the degree of the disease and the treatment. It is worthy of clinical reference.
Objective:To investigate the expression and clinical significance of NF-KB p65 protein in cutaneous squamous cell carcinoma. Methods:53 cases of skin squamous cell carcinoma were selected from January 2015 to January 2018 in our hospital,and 30 cases of normal skin tissues from January 2015 to January 2018 were selected as the control group. Immunohistochemical method was used to detect the expression of NF-kB p65 protein. The positive expression of NF-kB p65 protein in two groups was compared,and the positive expression of NF-kB p65 protein in different sex,different age,different clinical stages and different degree of differentiation. Results:The positive rate of NF-kB p65 protein in the study group was higher than that of the control group(P<0.05). The positive rate of NF-kB p65 in the squamous cell carcinoma of the skin of different sex and different age was not statistically different(P>0.05),and the positive rate of NF-kB p65 positive in III～IV stage squamous cell carcinoma of III～IV stage was higher than that of I～II and low differentiation. The positive expression rate of NF-kB p65 in cutaneous squamous cell carcinoma was higher than that in high and medium differentiation(P<0.05). Conclusion:NF-KB p65 protein is highly expressed in the squamous cell carcinoma of the skin,and the higher the state of the disease is,the higher the expression is,it is considered as a new target for judging the degree of the disease and the treatment. It is worthy of clinical reference.
引文
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[12]王璐,简玉洛,李钢,等.小分子干扰RNA沉默组蛋白去乙酰化酶1基因对皮肤鳞状细胞癌细胞增殖和凋亡的影响[J].中华实验外科杂志,2016,33(2):483-486.
[13]Strobel SB,Safferling K,Lahrmann B,et al. Altered density,composition and microanatomical distribution of infiltrating immune cells in cutaneous squamous cell carcinoma of organ transplant recipients[J]. Br J Dermatol,2018,179(2):405-412.
[14]Beck EM, Bauman TM, Rosman IS. A tale of two clones:Caldesmon staining in the differentiation of cutaneous spindle cell neoplasms[J]. J Cutan Pathol,2018,45(8):581-587.
[15]李美佳,张维娜,渠开攀,等.跨膜接头蛋白CBP对皮肤鳞癌细胞A431生长增殖的负调控作用研究[J].中国癌症杂志,2017,27(7):527-532.
[16]刘连庚,姜雪秋,张金蓉,等.皮肤鳞癌组织中E-Cadherin,p14ARF,P16INK4a基因启动子甲基化检测及其意义[J].现代检验医学杂志,2016,31(2):15-19.
[17]焦璐,王莹,吕游,等. NF-κB信号通路蛋白在木脂素对胃癌SGC-7901细胞抗增殖作用中的机制研究[J].时珍国医国药,2017,4(10):2392-2395.
[18]李玉英,牛敏,张立伟,等.新的蒽醌类衍生物1-硝基-2-酰基蒽醌-苯丙氨酸通过抑制NF-κB/p65信号通路降低乳腺癌MCF-7细胞的迁移能力[J].中国生物化学与分子生物学报,2017,33(3):269-277.
[19]朱文巍,赵梓纲,丁香玉,等.皮肤鳞状细胞癌NF-κB p65表达的临床价值[J].癌症进展,2016,14(5):492-493.
[20]邵雷,陈雄,黄耿,等.尿RANTES与LN活动性、肾组织RANTES mRNA和NF-kB p65 mRNA表达的相关性[J].热带医学杂志,2017,17(3):343-347.
[2]Gambichler T,Ruddel I,Hessam S,et al. Altered epigenetic pathways and cell cycle dysregulation in healthy appearing skin of patients with koebnerized squamous cell carcinomas following skin surgery[J]. J Eur Acard Dermatol Venereol,2018,32(9):1485-1491.
[3]Lam JKS,Sundaresan P,Gebski V,et al. Immunocompromised patients with metastatic cutaneous nodal squamous cell carcinoma of the head and neck:Poor outcome unrelated to the index lesion[J]. Head Neck,2018,40(5):985-992.
[4]Brugiere C,El Bouchtaoui M,Leboeuf C,et al. Perineural Invasion in Human Cutaneous Squamous Cell Carcinoma Is Linked to Neurotrophins,Epithelial-Mesenchymal Transition,and NCAM1[J]. J Invest Dermatol,2018,138(9):2063-2066.
[5]Stravodimou A,Tzelepi V,Papadaki H,et al. Evaluation of T-lymphocyte subpopulations in actinic keratosis,in situ and invasive squamous cell carcinoma of the skin[J]. J Cutan Pathol,2018,45(5):337-347.
[6]修丽娟,孙大志,刘宁宁,等.幽门螺杆菌阳性胃癌组织中RUNX3、NF-κB p65蛋白表达及临床意义[J].中华临床医师杂志(电子版),2016,13(1):11-15.
[7]夏庚,祁飞,杨晋辉,等.核因子-κB p65在肝细胞癌中的表达及意义[J].临床肝胆病杂志,2017,5(12):2342-2345.
[8]Hampras SS,Locke FL,Chavez JC,et al. Prevalence of cutaneous viral infections in incident cutaneous squamous cell carcinoma detected among chronic lymphocytic leukemia and hematopoietic stem cell transplant patients[J]. Leuk Lymphoma,2018,59(4):911-917.
[9]Tang JC,Kim SL,Stratman EJ. Measuring the Depth of Perineural Invasion in Cutaneous Squamous Cell Carcinoma:Implications on Biopsy Technique[J]. Dermatol Surg,2018. 44(9):1170-1173.
[10]Samsanavicius D,Kaikaris V,Cepas A,et al. Importance ofsentinellymphaticnodebiopsyindetectionofearly micrometastases in patients with cutaneous squamous cell carcinoma[J]. J Plast Reconstr Aesthet Surg,2018,71(4):597-603.
[11] Zhao CY,Hwang SJE,Anforth R,et al. Incidence of Basa l Cell Carcinoma and Squamous Cell Carcinoma in Patients on Antiprogrammed Cell Death-1 Therapy for Metastatic Melanoma[J]. J Immunother,2018,41(7):343-349.
[12]王璐,简玉洛,李钢,等.小分子干扰RNA沉默组蛋白去乙酰化酶1基因对皮肤鳞状细胞癌细胞增殖和凋亡的影响[J].中华实验外科杂志,2016,33(2):483-486.
[13]Strobel SB,Safferling K,Lahrmann B,et al. Altered density,composition and microanatomical distribution of infiltrating immune cells in cutaneous squamous cell carcinoma of organ transplant recipients[J]. Br J Dermatol,2018,179(2):405-412.
[14]Beck EM, Bauman TM, Rosman IS. A tale of two clones:Caldesmon staining in the differentiation of cutaneous spindle cell neoplasms[J]. J Cutan Pathol,2018,45(8):581-587.
[15]李美佳,张维娜,渠开攀,等.跨膜接头蛋白CBP对皮肤鳞癌细胞A431生长增殖的负调控作用研究[J].中国癌症杂志,2017,27(7):527-532.
[16]刘连庚,姜雪秋,张金蓉,等.皮肤鳞癌组织中E-Cadherin,p14ARF,P16INK4a基因启动子甲基化检测及其意义[J].现代检验医学杂志,2016,31(2):15-19.
[17]焦璐,王莹,吕游,等. NF-κB信号通路蛋白在木脂素对胃癌SGC-7901细胞抗增殖作用中的机制研究[J].时珍国医国药,2017,4(10):2392-2395.
[18]李玉英,牛敏,张立伟,等.新的蒽醌类衍生物1-硝基-2-酰基蒽醌-苯丙氨酸通过抑制NF-κB/p65信号通路降低乳腺癌MCF-7细胞的迁移能力[J].中国生物化学与分子生物学报,2017,33(3):269-277.
[19]朱文巍,赵梓纲,丁香玉,等.皮肤鳞状细胞癌NF-κB p65表达的临床价值[J].癌症进展,2016,14(5):492-493.
[20]邵雷,陈雄,黄耿,等.尿RANTES与LN活动性、肾组织RANTES mRNA和NF-kB p65 mRNA表达的相关性[J].热带医学杂志,2017,17(3):343-347.
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