巴什拜羊H-FABP基因遗传多态性及其与生长性状的相关分析
|
摘要
旨在运用分子生物学手段研究H-FABP基因遗传多态性与巴什拜羊生长性状的关系,为今后用分子标记辅助育种方法提高巴什拜羊生长性状提供科学依据。以300只巴什拜周岁母羊为研究对象,选取H-FABP基因外显子2和外显子3的部分片段,采用PCR-SSCP和DNA测序等技术检测其遗传多态性,并与体重、体长、体高等性状进行关联分析,研究H-FABP基因的多态性与巴什拜羊生长性状的关系。结果表明,H-FABP-P_2基因座外显子3无多态性;H-FABP-P_1基因座外显子2存在AA和AG2种基因型,测序结果显示在H-FABP基因外显子2的938bp处发生了A→G碱基突变,为同义突变。经关联分析发现,AG基因型个体的胸围和体重显著高于AA基因型个体(P<0.05)。因此,巴什拜羊H-FABP基因外显子2上的点突变可能是影响巴什拜羊生长性状的重要位点。
In this study, in order to provide scientific basis for improving growth traits of Bashibai sheep by molecular marker assisted breeding methods, the relationship between the genetic polymorphisms of H-FABP gene and the growth traits was studied by molecular biology methods. In this experiment, 300 Bashibai yearling ewes were taken to select part of segments of H-FABP gene exon 2 and exon 3 and study their genetic polymorphisms by PCR-SSCP and DNA sequencing. Its association with body weight, body length, and body height was then explored to study the relationship between H-FABP gene polymorphism and these indexes of Bashibai sheep. Results show there was no polymorphism in H-FABP-P_2 gene locus(exon3); there was polymorphism in H-FABP-P_1 Gene locus(exon 2), which had 2 genotypes: AA and AG. Sequencing results showed that there was a base mutation A →G in location 938 bp of H-FABP gene exon 2, which was synonymous mutation. Correlation analysis found that the individual weight and heart girth of AG genotype were much higher than that of AA genotype(P<0.05). The point mutation on exon 2 of H-FABP gene in Bashibai sheep may be an important site affecting the growth traits of Bashibai sheep.
In this study, in order to provide scientific basis for improving growth traits of Bashibai sheep by molecular marker assisted breeding methods, the relationship between the genetic polymorphisms of H-FABP gene and the growth traits was studied by molecular biology methods. In this experiment, 300 Bashibai yearling ewes were taken to select part of segments of H-FABP gene exon 2 and exon 3 and study their genetic polymorphisms by PCR-SSCP and DNA sequencing. Its association with body weight, body length, and body height was then explored to study the relationship between H-FABP gene polymorphism and these indexes of Bashibai sheep. Results show there was no polymorphism in H-FABP-P_2 gene locus(exon3); there was polymorphism in H-FABP-P_1 Gene locus(exon 2), which had 2 genotypes: AA and AG. Sequencing results showed that there was a base mutation A →G in location 938 bp of H-FABP gene exon 2, which was synonymous mutation. Correlation analysis found that the individual weight and heart girth of AG genotype were much higher than that of AA genotype(P<0.05). The point mutation on exon 2 of H-FABP gene in Bashibai sheep may be an important site affecting the growth traits of Bashibai sheep.
引文
[1] CHMURZYNSKA A.The multigen family of fatty acid-binding proteins(FABPs):Function structure and polymorphism[J].Journal of Applied Genetics,2006,47(1):39-48.
[2] GERBENS F,VERBURG F J,VAN MOERKERK H T,et al.Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs[J].Journal of Animal Science,2001,79(2):347.
[3] SAMULIN J,BERGET I,LIEN S,et al.Differential gene expression of fatty acid binding proteins during porcine adipogenesis[J].Comparative Biochemistry and Physiology (Part B),2008,151(2):147-152.
[4] CALVO J H,VAIMAN D,SAIDI-MEHTAR N,et al.Characterization,genetic variation and chromosomal assignment to sheep chromosome 2 of the ovine heart fatty acid-binding protein gene (FABP3)[J].Cytogenetic and Genome Research,2002,98:270-273.
[5] 余刚,罗军,韩雪峰,等.陕北白绒山羊H-FABP基因SNPs及其与生长、胴体性状的相关研究[J].畜牧兽医学报,2007,38(11):1 154-1 159.
[6] HUANG Z G,XIONG L,LIU Z S,et al.The developmental changes and effect on IMF content of H-FABP and PPARγ mRNA expression in sheep muscle[J].Acta Cenetica Sinica,2006,33(6):507-514.
[7] 买买提明·巴拉提,哈米提·哈凯莫夫,决肯·阿尼瓦什.羔羊肉型巴什拜羊的研究[J].草食家畜,1999(2):16-19.
[8] 努尔拉提汗·木哈买提别克,赛力克·木勒达汗,决肯·阿尼瓦什,等.新疆巴什拜羊青色和白色毛绒品质比较分析[J].新疆畜牧业,2015(11):25-27.
[9] 罗燕,谷新利,赵宗胜,等.中国美利奴羊H-FABP基因多态性及其与部分肉质性状的相关性[J].动物学杂志,2013,48(6):964-970.
[10] 赵雪.绵羊H-FABP基因单核苷酸多态性及其与肉用性状的关联分析[D].延吉:延边大学,2013.
[11] 周延清,杨清香,张改娜,等.生物遗传标记与应用[M].北京:化学工业出版社,2008:104-105.
[12] 张英杰.羊生产学[M].北京:中国农业出版社,2010:252-253.
[13] 乔海云.滩羊H-FABP和ADD1基因遗传多态性及其与肌肉脂肪含量的关联分析[D].北京:中国农业科学院,2009.
[14] 钱成,罗卫星,孙岩岩,等.贵州地方山羊品种H-FABP基因第3外显子SNP研究[J].生物技术,2015(4):349-354.
[15] 余刚.陕北白绒山羊H-FABP基因内含子3的遗传多态性分析[J].中国草食动物,2010,30(1):5-8.
[16] 任法鑫,杨钧国,李伟,等.国人SCN5A基因新的同义突变位点[J].中国循环杂志,2004(5):52-54.
[17] 陈南春,高辉,陈苏民,等.cⅠ857基因的体外定位同义突变[J].生物化学杂志,1994(4):509-512.
[18] CORRADO L,MAGRI S,BAGAROTTI A,et al.A novel synonymous mutation in the MPZ gene causing an aberrant splicing pattern and Charcot-Marie-Tooth disease type 1b[J].Neuromuscular Disorders,2016,26(8):516-520.
[19] 周明亮,张翔宇,綦松智,等.绵羊IGF-Ⅰ基因的表达及其SNPs与生长性状的相关分析研究[J].畜牧兽医学报,2013,44(9):1 363-1 370.
[20] 宋桃伟,蔡慧芬,罗卫星,等.两个贵州山羊品种GHSR和GHRL基因遗传变异及其与生长性状的关联性[J].中国农业科学,2015,48(l):140-153.
[2] GERBENS F,VERBURG F J,VAN MOERKERK H T,et al.Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs[J].Journal of Animal Science,2001,79(2):347.
[3] SAMULIN J,BERGET I,LIEN S,et al.Differential gene expression of fatty acid binding proteins during porcine adipogenesis[J].Comparative Biochemistry and Physiology (Part B),2008,151(2):147-152.
[4] CALVO J H,VAIMAN D,SAIDI-MEHTAR N,et al.Characterization,genetic variation and chromosomal assignment to sheep chromosome 2 of the ovine heart fatty acid-binding protein gene (FABP3)[J].Cytogenetic and Genome Research,2002,98:270-273.
[5] 余刚,罗军,韩雪峰,等.陕北白绒山羊H-FABP基因SNPs及其与生长、胴体性状的相关研究[J].畜牧兽医学报,2007,38(11):1 154-1 159.
[6] HUANG Z G,XIONG L,LIU Z S,et al.The developmental changes and effect on IMF content of H-FABP and PPARγ mRNA expression in sheep muscle[J].Acta Cenetica Sinica,2006,33(6):507-514.
[7] 买买提明·巴拉提,哈米提·哈凯莫夫,决肯·阿尼瓦什.羔羊肉型巴什拜羊的研究[J].草食家畜,1999(2):16-19.
[8] 努尔拉提汗·木哈买提别克,赛力克·木勒达汗,决肯·阿尼瓦什,等.新疆巴什拜羊青色和白色毛绒品质比较分析[J].新疆畜牧业,2015(11):25-27.
[9] 罗燕,谷新利,赵宗胜,等.中国美利奴羊H-FABP基因多态性及其与部分肉质性状的相关性[J].动物学杂志,2013,48(6):964-970.
[10] 赵雪.绵羊H-FABP基因单核苷酸多态性及其与肉用性状的关联分析[D].延吉:延边大学,2013.
[11] 周延清,杨清香,张改娜,等.生物遗传标记与应用[M].北京:化学工业出版社,2008:104-105.
[12] 张英杰.羊生产学[M].北京:中国农业出版社,2010:252-253.
[13] 乔海云.滩羊H-FABP和ADD1基因遗传多态性及其与肌肉脂肪含量的关联分析[D].北京:中国农业科学院,2009.
[14] 钱成,罗卫星,孙岩岩,等.贵州地方山羊品种H-FABP基因第3外显子SNP研究[J].生物技术,2015(4):349-354.
[15] 余刚.陕北白绒山羊H-FABP基因内含子3的遗传多态性分析[J].中国草食动物,2010,30(1):5-8.
[16] 任法鑫,杨钧国,李伟,等.国人SCN5A基因新的同义突变位点[J].中国循环杂志,2004(5):52-54.
[17] 陈南春,高辉,陈苏民,等.cⅠ857基因的体外定位同义突变[J].生物化学杂志,1994(4):509-512.
[18] CORRADO L,MAGRI S,BAGAROTTI A,et al.A novel synonymous mutation in the MPZ gene causing an aberrant splicing pattern and Charcot-Marie-Tooth disease type 1b[J].Neuromuscular Disorders,2016,26(8):516-520.
[19] 周明亮,张翔宇,綦松智,等.绵羊IGF-Ⅰ基因的表达及其SNPs与生长性状的相关分析研究[J].畜牧兽医学报,2013,44(9):1 363-1 370.
[20] 宋桃伟,蔡慧芬,罗卫星,等.两个贵州山羊品种GHSR和GHRL基因遗传变异及其与生长性状的关联性[J].中国农业科学,2015,48(l):140-153.