结合原子力显微测量的多功能包膜造影剂机械特性研究
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- 英文论文题名：Mechanical properties of hybrid contrast agents combing the measurements using atomic force microscopy
- 论文作者：郭各朴 ; 高雅 ; 马青玉
- 英文论文作者：GUO Ge-pu ; GAO Ya ; MA Qing-yu ; Key Laboratory of Optoelectronics of Jiangsu Province ; School of Physics and Technology ; Nanjing Normal University
- 年：2016
- 作者机构：南京师范大学物理科学与技术学院;
- 论文关键词：原子力显微技术 ; 机械特性 ; 多功能包膜造影剂 ; 超顺磁纳米颗粒
- 英文论文关键词：Atomic force microscopy ; Mechanical property characterization ; Hybrid US/MR imaging contrast agents ; superparamagnetic iron oxide nanoparticles
- 会议召开时间：2016-10-28
- 会议录名称：2016年全国声学学术会议论文集
- 语种：中文
- 分类号：TH77
- 学会代码：OGSM
- 会议名称：2016年全国声学学术会议
- 会议地点：中国湖北武汉
- 主办单位：中国声学学会
- 学会名称：中国声学学会
- 页数：5
- 文件大小：423k
- 原文格式：D
- 会议级别：全国
摘要
基于多参数动力学模型拟合实验数据推测造影剂微泡包膜特性的方法,不同形式动力学模型的选用存在较大的不确定性。本文提出了一种综合性技术来定征多功能包膜微泡的机械特性和声学特性,并提高评估的准确性和确定性。将原子力显微技术,单微粒光感技术,声衰减特性,包膜气泡动力学仿真理论相结合,通过多功能造影剂微泡的粒径分布,包膜厚度和粘弹特性的测量,消除模型的不确定性。结果表明随着SPIO纳米颗粒浓度的增加,包膜微泡的粒径分布逐渐增大,共振频率降低;粘性系数呈上升趋势,而弹性系数呈下降趋势,造影剂的散射特性逐渐增强。
As the often used method for the characterization of coated-bubbles, multi-parameter fitting algorithms introduce uncertainty into the results inevitably. Therefore, improved techniques that can accurately determine the microbubbles' mechanical properties are needed urgently. A comprehensive technology combining atomic force microscopy, optical, and acoustic measurements with simulations of coated-bubble dynamics was developed. The mechanical parameters(size distribution, shell thickness, elasticity, and viscosity) of the hybrid(ultrasound/magnetic- resonance-imaging) contrast microbubbles and their structure-property relationship were determined. The measurements indicate that when more superparamagnetic iron oxide nanoparticles are embedded in the microbubbles' shell, the mean diameter and effective viscosity increase, and the elastic modulus decreases accordingly. This property reduces the microbubbles' resonance frequency and thus enhances acoustic scattering and attenuation effects.
As the often used method for the characterization of coated-bubbles, multi-parameter fitting algorithms introduce uncertainty into the results inevitably. Therefore, improved techniques that can accurately determine the microbubbles' mechanical properties are needed urgently. A comprehensive technology combining atomic force microscopy, optical, and acoustic measurements with simulations of coated-bubble dynamics was developed. The mechanical parameters(size distribution, shell thickness, elasticity, and viscosity) of the hybrid(ultrasound/magnetic- resonance-imaging) contrast microbubbles and their structure-property relationship were determined. The measurements indicate that when more superparamagnetic iron oxide nanoparticles are embedded in the microbubbles' shell, the mean diameter and effective viscosity increase, and the elastic modulus decreases accordingly. This property reduces the microbubbles' resonance frequency and thus enhances acoustic scattering and attenuation effects.
引文
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[2]Michalet X,Pinaud F,Bentolila L.Quantum dots for live cells,in vivo Imaging,and Diagnostics[J].Science,2005,307(5709):538-544
[3]Baker M.Whole-animal imaging:the whole picture[J].Nature,2010,463:977–980.
[4]Louie Y.Multimodality imaging probes:design and challenges[J].Chemical Reviews.2010,110:3146–3195.
[5]Sailor MJ,Park JH.Hybrid nanoparticles for detection and treatment of diagnostics[J].Science,2012,307:538–544.
[6]Niu C,Wang Z,Lu G,Krupka T,Sun Y,You Y,Song W,Ran H,Li P,Zheng Y.Doxorubicin loaded superparamagnetic PLGA-Iron Oxide multifunctional microbubbles for dual-mode US/MR imaging and therapy of metastasis in lymph nodes[J].Biomaterials,2013,34:2307–2317.
[7]Guo XS,Li Q,Zhang Z,Zhang D,Tu J.Investigation on the inertial cavitation threshold and shell properties of commercialized ultrasound contrast agent microbubbles[J].Journal of the Acoustical Society of America,2013,134:1622–1631.
[8]Fouan D,Achaoui Y,Mensah S.Improvement of micro-bubble sizing using multi-harmonic excitations under the transducer bandwidth constraint[J].Applied Physics Letters,2014,104:114102.
[9]Guo GP,Lu L,Ji HF,Ma Y,Dong R,Tu J,Guo XS,Qiu YY,Wu JR,Zhang D.Low intensity pulse ultrasound stimulate chondrocytes growth in a 3-D alginate scaffold through improved porosity and permeability,Ultrasonics,2015,58:43-52.
[10]de Jong N,Hoff L.Ultrasound scattering properties of Albunex microspheres[J].Ultrasonics,1993,31:175-181.
[11]Marmottant P,van der Meer S,Emmer M,Versluis M,de Jong N,Hilgenfeldt S,Lohse D.A model for large amplitude oscillations of coated bubbles accounting for buckling and rupture[J].Journal of the Acoustical Society of America,2005,118:3499-3505.
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[13]Fouan D,Achaoui Y,Mensah S.Improvement of micro-bubble sizing using multi-harmonic excitations under the transducer bandwidth constraint[J].Applied Physics Letters,2014,104:114102.