轮作与连作对烤烟不同生育期根际土壤细菌群落结构的影响
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- 英文论文题名：Effects of rotation and continuous cropping of flue-cured tobacco on rhizosphere bacterial community structures in the at different growth stages
- 论文作者：元野 ; 李莞晴 ; 郭健 ; 秦成章 ; 程晓东 ; 贺国强
- 英文论文作者：Yuan Ye ; Li Wanqing ; Guo Jian ; Qin Chengzhang ; Cheng Xiaodong ; He Guoqiang ; Mudanjang Tobacco Science Research Institute ; Heilongjiang Province Tobacco Monopoly Bureau ; Fujin Branch of Harbin Tobacco Leaf Cooperation ; Tangyuan Branch of Harbin Tobacco Leaf Cooperation ; Hailin Branch of Mudanjiang Tobacco Leaf Cooperation
- 年：2016
- 作者机构：牡丹江烟草科学研究所;黑龙江省烟草专卖局;哈尔滨烟叶公司富锦分公司;哈尔滨烟叶公司汤原分公司;牡丹江烟叶公司海林分公司;
- 论文关键词：454焦磷酸测序 ; 多样性指数 ; β-多样性 ; 细菌群落 ; 烤烟连作
- 英文论文关键词：454 pyrosequencing ; diversity index ; Bata diversity ; bacterial community ; continuous cropping of flue-cured tobacco
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S154.3;S572
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：9
- 文件大小：1614k
- 原文格式：D
摘要
为明晰烤烟连作是否对根际细菌群落结构产生影响,采集不同生育时期的轮作和连作烤烟根际土壤,运用454焦磷酸测序技术,对根际土壤细菌16S rDNA V1-V3片段序列结构组成进行分析。结果表明,较轮作处理相比,连作处理烤烟根际酸杆菌门丰度在生育前期低而在后期高,但α-变形菌门和放线菌门表现出相反的变化趋势;烤烟连作降低了表征菌群丰度的Chao指数和物种丰富度的Shannon指数,表明烤烟连作降低了根际土壤细菌多样性。主成分分析和NMDS(non-metric multidimensional scaling)的聚类分析结果显示不同处理烤烟根际细菌群落结构随生育期而迁移,轮作与连作烤烟根际土壤细菌群落结构差异在生育前期体现明显,生育后期不明显,表明生育期是影响烤烟根际细菌群落结构变化的主要因素,而连作与轮作是次要因素。该研究结果为明确烟草连作障碍的土壤微生物学机制提供了理论参考。
To explore the whether the effects of continuous cropping of flue-cured tobacco on rhizosphere bacterial community structures, the rhizosphere soils of rotation and continuous cropping of tobacco at different growth stages were collected, and the V1-V3 partial sequences of bacterial 16 S r DNA were analyzed using 454 pyrosequencing method. Results showed that, compared with the rotation treatments, the relative abundance of Acidobacteria was higher in earlier growth stages and lower in later stages in the continuous cropping of flue-cured tobacco, while the relative abundances of α-proteobacteria and Actinobacteria were in the opposite change tendency. The community richness(Chao) and diversity(Shannon) in continuous cropping tobacco treatment were lower than these in the rotation systems, suggesting that the continuous cropping decreased the bacterial community diversity. The principal component analysis and the cluster analysis based on non-metric multidimensional scaling showed that the bacterial communities changed with tobacco growth stages, and the difference of community between continuous cropping and rotation was detected in the earlier growth stages, not in the later stages, suggested that the growth stages was the primary factor and the continuous cropping or rotation was the second factor in determining the tobacco rhizosphere bacterial community. In general, the results of this study provided the microbial information to understand the barrier mechanisms of continuous cropping of tobacco.
To explore the whether the effects of continuous cropping of flue-cured tobacco on rhizosphere bacterial community structures, the rhizosphere soils of rotation and continuous cropping of tobacco at different growth stages were collected, and the V1-V3 partial sequences of bacterial 16 S r DNA were analyzed using 454 pyrosequencing method. Results showed that, compared with the rotation treatments, the relative abundance of Acidobacteria was higher in earlier growth stages and lower in later stages in the continuous cropping of flue-cured tobacco, while the relative abundances of α-proteobacteria and Actinobacteria were in the opposite change tendency. The community richness(Chao) and diversity(Shannon) in continuous cropping tobacco treatment were lower than these in the rotation systems, suggesting that the continuous cropping decreased the bacterial community diversity. The principal component analysis and the cluster analysis based on non-metric multidimensional scaling showed that the bacterial communities changed with tobacco growth stages, and the difference of community between continuous cropping and rotation was detected in the earlier growth stages, not in the later stages, suggested that the growth stages was the primary factor and the continuous cropping or rotation was the second factor in determining the tobacco rhizosphere bacterial community. In general, the results of this study provided the microbial information to understand the barrier mechanisms of continuous cropping of tobacco.
引文
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[2]吴凤芝,赵凤艳,刘元英.设施蔬菜连作障碍原因综合分析与防治措施[J].东北农业大学学报,2000,31(3):241-247.
[3]杨宇虹,陈冬梅,晋艳,等.连作烟草对土壤微生物区系影响的T-RFLP分析[J].中国烟草学报,2012,18(1):40-45.
[4]Aleman F.Common bean response to tillage intensity and weed control strategies[J].Agron J,2001,93:556-563.
[5]张淑香,高子勤.连作障碍与根际微生态研究II.根系分泌物与酚酸物质[J].应用生态学报,2000,11(1):152-156.
[6]丁海兵.连作烤烟生长及不同粒级土壤酶活性的影响[D].重庆.西南大学2006.
[7]张心昱,陈利顶,傅伯杰,等.农田生态系统不同土地利用方式与管理措施对土壤质量的影响[J].应用生态学报,2007,18(2):303-309.
[8]Rappe M S,Giovannoni S J.The uncultured microbial majority[J].Rev Microbiol,2003,57:369-394.
[9]Ward D M,Weller R,Bateson M M.16S r RNA sequences reveal numerous uncultured microorganisms in a nature community[J].Nature,1990,344:63-65.
[10]Ferris M J,Muyzer G,Ward D M.Denaturing gradient gel electrophoresis profiles of 16Sr RNA defined populations inhabiting a hotspring microbial mat community[J].Appl Environ Microbiol,1996,62(2):340-346.
[11]Muyzer G,de-Waal E C,Uitterlinden A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S r RNA[J].Appl Environ Microbiol,1993,59(3):695-700.
[12]Sun Y J,Cai Y P,Liu L.ESPRIT:estimating species richness using large collections of 16S r RNA pyrosequences[J].Nucleic Acids Res,2009,37(10):e76.
[13]薛超,黄启为,凌宁,等.连作土壤微生物区系分析、调控及高通量研究方法[J].土壤学报,2011,48(3):612-616.
[14]Rafael S,Thomas B,Alexander G,et al.Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology[J].J Biotechnol,2008,136:54-64.
[15]Baker G,Smith JJ,Cowan DA,et al.Review and re-analysis of domain-specific 16S primers[J].J Microbiol Meth,2003,55:541-555.
[16]Dohm J C,Lottaz C,Borodina T,et al.Substantial biases in ultra-short read data sets from high-throughput DNA sequencing[J].Nucleic Acids Res,2008,36:1-10.
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