冷藏期间水分胁迫对菠菜叶片衰老生理的影响
详细信息 查看官网全文
- 英文论文题名：Effects of Water Stress on Senescence Physiology of Spinach Leaves at Low Storage Temperatures
- 论文作者：田莹 ; 侯建设 ; 李丹
- 英文论文作者：TIAN Ying ; HOU Jian-she ; LI Dan ; Naval Medical Institute of PLA
- 年：2014
- 作者机构：中国人民解放军海军医学研究所;
- 论文关键词：菠菜叶片 ; 冷藏 ; 水分胁迫 ; 活性氧代谢 ; 衰老
- 英文论文关键词：pinach blade ; cold storage ; water stress ; activated oxygen metabolism ; senescence
- 会议召开时间：2014-11-27
- 会议录名称：第九届全国食品冷藏链大会暨第六届全国冷冻冷藏产业创新发展年会论文集
- 语种：中文
- 分类号：TS255.3
- 学会代码：GDKL
- 会议名称：第九届全国食品冷藏链大会暨第六届全国冷冻冷藏产业创新发展年会
- 会议地点：中国广东广州
- 主办单位：中国制冷学会、国内贸易工程设计研究院
- 学会名称：广东省科学技术协会科技交流部
- 页数：7
- 文件大小：905k
- 原文格式：O
- 会议级别：全国
摘要
保水处理菠菜以低密度聚乙烯(LDPE)薄膜袋自然合拢不扎口包装,对照菠菜直接装在多孔塑料筐内,在RH80%、温度(2±1)℃下贮藏,研究冷藏期间大量失水(水分胁迫)对菠菜叶片叶绿素降解和活性氧代谢的影响。结果表明,冷藏期间对照菠菜失水很快,遭受严重水分胁迫;而保水处理菠菜失水很少。与保水处理菠菜相比,水分胁迫加快了对照菠菜叶片叶绿素含量的下降速度,使超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性降低,其中CAT、和APX活性降低速率超过SOD,促进了过氧化物酶(POD)活性的上升,但没有明显增加丙二醛(MDA)的积累。说明水分胁迫降低了采后菠菜贮藏期间活性氧清除酶的活性,导致氧化胁迫,从而加速叶绿素的降解和衰老。
Effects of water stress on chlorophyll degradation and activated oxygen metabolism in Spinach Leaves during cold storage was studied.Spinaches packed directly in plastic skeps as control,preserving-water-treated spinaches was packed in plastic film bag without sealed and stored at 2℃,During cold storage controlled spinaches lost water very quickly and suffered severe water stress,while preserving-water-treated groups of the vegetables lost water little.Compared to preserving-water-treated ones,water stress accelerated the decline in chlorophyll content in blade of spinach,enhanced the decrease in activities of SOD,CAT,APX and GR in blades,and the activity of CAT and APX was decreased much more than SOD,but didn't enhance accumulation in MDA content.The results indicate water stress enhance the decrease in activated oxygen scavenging enzyme activity,leading to oxidative stress,therefore,quickening chlorophyll degradation and senescence.
Effects of water stress on chlorophyll degradation and activated oxygen metabolism in Spinach Leaves during cold storage was studied.Spinaches packed directly in plastic skeps as control,preserving-water-treated spinaches was packed in plastic film bag without sealed and stored at 2℃,During cold storage controlled spinaches lost water very quickly and suffered severe water stress,while preserving-water-treated groups of the vegetables lost water little.Compared to preserving-water-treated ones,water stress accelerated the decline in chlorophyll content in blade of spinach,enhanced the decrease in activities of SOD,CAT,APX and GR in blades,and the activity of CAT and APX was decreased much more than SOD,but didn't enhance accumulation in MDA content.The results indicate water stress enhance the decrease in activated oxygen scavenging enzyme activity,leading to oxidative stress,therefore,quickening chlorophyll degradation and senescence.
引文
[1]Impa S M S,Nadaradjan S,Jagadish S V K.Drought stress induced reactive oxygen species and anti-oxidants in plants[M]//Ahmad P,Prasad.Abiotic Stress Responses in Plants:Metabolism,Productivity and Sustainability.New York:Springer Science+Business Media,2012:131-147.
[2]Jiang Y M,Fu J R.Postharvest browning of litchi fruit by water loss and its prevention by controlled atmosphere storage at high relative humidity[J].Lebesmittel Wissenschaft and Technologic 1999,32(5):278-283.
[3]侯建设,李中华,席玙芳.水分胁迫对冷藏韭菜叶片衰老和活性氧代谢的影响[J].食品与发酵工业.2005,31(5):143-146.
[4]李丹,侯建设,田莹.采后失水对芹菜叶柄黄化和氧化胁迫的影响[J].食品工业科技,2010(2):314-316.
[5]田莹,侯建设,李丹.不同冷藏温度对采后菠菜叶片衰老和活性氧代谢的影响[J].保鲜与加工,2013,13(1):9-14.
[6]何宇炯,徐如涓,赵毓橘,等.表油菜素内酯对绿豆幼叶衰老的促进作用[J].植物生理学报,1996,22(1):58-62.
[7]Nakano Y,Asada K.Purification of ascorbate peroxidase in spinach chloroplasts;its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical[J].Plant and Cell Physiology,1987,28(1):131-134.
[8]Zhang J X,Kirkham M B.Antioxidant responses to drought in sunflower and sorghum seedlings[J].New Phytology,1996,132(3):361-372.
[9]吴登如,赵毓橘.表油菜素内酯对绿豆上胚轴内源IAA及其氧化酶的影响[J].植物生理学报,1991(4):327-332.
[10]朱广廉.植物生理学实验[M].北京:北京大学出版社,1990:51-53.
[11]潘增光,王国宾,李奎明,等.新红星苹果果实着色期几种色素含量变化及其相关性(简报)[J].植物生理学通讯,1996,32(5):347-349.
[12]陈立松,刘星辉.水分胁迫对荔枝叶片活性氧代谢的影响[J].园艺学报,1998,25(3):34-39.
[13]何承坤,郭素枝,李家慎.干旱胁迫对番茄活性氧代谢的影响[J].福建农业大学学报,1996,25(3):58-62.
[14]罗华建,刘星辉.谢厚钗.水分胁迫对枇杷叶片活性氧代谢的影响[J].福建农业大学学报,1999,28(1):33-37.
[15]Devi R,Kaur N,Gupta A K.Potential of antioxidant enzymes in depicting drought tolerance of wheat(Triticum aestivum L.)[J].Indian Journal Biochemistry and Biophysics,2012,49(4):257-265.
[16]Doupis G,BertakiM,PsarrasG,et al.Water relations,physiological behavior and antioxidant defence mechanism of olive plants subjected to different irrigation regimes[J].Scientia Horticulturae,2013,153:150-156.
[17]Matile P,Schellenberg M,Vicentini F.Localization of chlorophyllase in the chloroplast envelope[J].Planta,1997,201(1):96-99.
[18]Matile P,Schellenberg M.The cleavage of pheophorbide a is located in the envelope of barley gerontoplasts[J].Plant Physiology and Biochemistry,1996,34:55-59.
[19]Vicentini F,Hortensteiner S,Schellenberg M,et al.Chloropyll breakdown in senescent leaves:identification of the biochemical lesion in a stay-green genotype of Festuca pratensis[J].New Phytologist,1995,129(2):247-252.
[20]曾韶西,王以柔,刘鸿光.低温光照下与黄瓜子叶叶绿素降低有关的酶促反应[J].植物生理学报,1991,17(2):177-182.
[21]Kar R K,Choudhuri M A.Possible mechanisms of light-induced chlorophyll degradation in senescing leaves of Hytrilla verticillata[J].Physiologia Plantarum,1987.70(4):729-734.
[22]Kaur K,Kaur N,Gupta A K,et al.Exploration of the antioxidative defense system to characterize chickpea genotypes showing differential response towards water deficit conditions[J].Plant Growth Regul,2013,70(1):49-60.
[23]王邦锡,孙莉,黄久常.渗透胁迫引起的膜损伤与膜脂过氧化和某些自由基的关系[J].中国科学(B辑化学生命科学地学),1992(4):364-368.
[24]Senaratna T,McKersie B D,Stinson R H.Association between membrane phase properties and dehydration injury in soybean axes[J].Plant Physiology,1984,76(3):759-762.
[25]Senaratna T,McKersie B D,Stinson R H.Simulation of dehydration injury to membranes from soybean axes by free radical[J].Plant Physiology,1985,77(2):472-474.
[26]Senaratna T,McKersie B D,Borochov A.Desication and free radical mediated changes in plant membranes[J].Journal of Experimental Botany,1987,38(12):2005-2014.
[27]吕庆,郑荣梁.干旱及活性氧引起小麦膜脂过氧化与脱酯化[J].中国科学(C辑:生命科学),1996,26(1):26-30.
[2]Jiang Y M,Fu J R.Postharvest browning of litchi fruit by water loss and its prevention by controlled atmosphere storage at high relative humidity[J].Lebesmittel Wissenschaft and Technologic 1999,32(5):278-283.
[3]侯建设,李中华,席玙芳.水分胁迫对冷藏韭菜叶片衰老和活性氧代谢的影响[J].食品与发酵工业.2005,31(5):143-146.
[4]李丹,侯建设,田莹.采后失水对芹菜叶柄黄化和氧化胁迫的影响[J].食品工业科技,2010(2):314-316.
[5]田莹,侯建设,李丹.不同冷藏温度对采后菠菜叶片衰老和活性氧代谢的影响[J].保鲜与加工,2013,13(1):9-14.
[6]何宇炯,徐如涓,赵毓橘,等.表油菜素内酯对绿豆幼叶衰老的促进作用[J].植物生理学报,1996,22(1):58-62.
[7]Nakano Y,Asada K.Purification of ascorbate peroxidase in spinach chloroplasts;its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical[J].Plant and Cell Physiology,1987,28(1):131-134.
[8]Zhang J X,Kirkham M B.Antioxidant responses to drought in sunflower and sorghum seedlings[J].New Phytology,1996,132(3):361-372.
[9]吴登如,赵毓橘.表油菜素内酯对绿豆上胚轴内源IAA及其氧化酶的影响[J].植物生理学报,1991(4):327-332.
[10]朱广廉.植物生理学实验[M].北京:北京大学出版社,1990:51-53.
[11]潘增光,王国宾,李奎明,等.新红星苹果果实着色期几种色素含量变化及其相关性(简报)[J].植物生理学通讯,1996,32(5):347-349.
[12]陈立松,刘星辉.水分胁迫对荔枝叶片活性氧代谢的影响[J].园艺学报,1998,25(3):34-39.
[13]何承坤,郭素枝,李家慎.干旱胁迫对番茄活性氧代谢的影响[J].福建农业大学学报,1996,25(3):58-62.
[14]罗华建,刘星辉.谢厚钗.水分胁迫对枇杷叶片活性氧代谢的影响[J].福建农业大学学报,1999,28(1):33-37.
[15]Devi R,Kaur N,Gupta A K.Potential of antioxidant enzymes in depicting drought tolerance of wheat(Triticum aestivum L.)[J].Indian Journal Biochemistry and Biophysics,2012,49(4):257-265.
[16]Doupis G,BertakiM,PsarrasG,et al.Water relations,physiological behavior and antioxidant defence mechanism of olive plants subjected to different irrigation regimes[J].Scientia Horticulturae,2013,153:150-156.
[17]Matile P,Schellenberg M,Vicentini F.Localization of chlorophyllase in the chloroplast envelope[J].Planta,1997,201(1):96-99.
[18]Matile P,Schellenberg M.The cleavage of pheophorbide a is located in the envelope of barley gerontoplasts[J].Plant Physiology and Biochemistry,1996,34:55-59.
[19]Vicentini F,Hortensteiner S,Schellenberg M,et al.Chloropyll breakdown in senescent leaves:identification of the biochemical lesion in a stay-green genotype of Festuca pratensis[J].New Phytologist,1995,129(2):247-252.
[20]曾韶西,王以柔,刘鸿光.低温光照下与黄瓜子叶叶绿素降低有关的酶促反应[J].植物生理学报,1991,17(2):177-182.
[21]Kar R K,Choudhuri M A.Possible mechanisms of light-induced chlorophyll degradation in senescing leaves of Hytrilla verticillata[J].Physiologia Plantarum,1987.70(4):729-734.
[22]Kaur K,Kaur N,Gupta A K,et al.Exploration of the antioxidative defense system to characterize chickpea genotypes showing differential response towards water deficit conditions[J].Plant Growth Regul,2013,70(1):49-60.
[23]王邦锡,孙莉,黄久常.渗透胁迫引起的膜损伤与膜脂过氧化和某些自由基的关系[J].中国科学(B辑化学生命科学地学),1992(4):364-368.
[24]Senaratna T,McKersie B D,Stinson R H.Association between membrane phase properties and dehydration injury in soybean axes[J].Plant Physiology,1984,76(3):759-762.
[25]Senaratna T,McKersie B D,Stinson R H.Simulation of dehydration injury to membranes from soybean axes by free radical[J].Plant Physiology,1985,77(2):472-474.
[26]Senaratna T,McKersie B D,Borochov A.Desication and free radical mediated changes in plant membranes[J].Journal of Experimental Botany,1987,38(12):2005-2014.
[27]吕庆,郑荣梁.干旱及活性氧引起小麦膜脂过氧化与脱酯化[J].中国科学(C辑:生命科学),1996,26(1):26-30.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |