基于宏基因组学技术的臭鳜鱼微生物群落结构分析及安全性评价研究

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• 英文论文题名：Metagenomic analysis of microbial ecosystem and safety in stinky mandarin fish
• 论文作者：刘洒洒 ; 林心萍 ; 高宁 ; 纪超凡 ; 董秀萍
• 英文论文作者：Liu Sasa ; Lin Xinping ; Gao Ning ; Ji Chaofan ; Dong Xiuping ; College of Food Science ; Dalian Polytechnic University ; Nationl Engineering Research Center of Seafood
• 年：2016
• 作者机构：大连工业大学食品学院;国家海洋食品工程技术研究中心;
• 论文关键词：臭鳜鱼 ; 菌群结构 ; 宏基因组学技术 ; 分离鉴定 ; 安全性
• 英文论文关键词：stinky mandarin fish ; microbial ecosystem ; metagenomics ; lsolation and identification ; safety
• 会议召开时间：2016-11-09
• 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
• 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
• 语种：中文
• 分类号：Q93;TS254.1
• 学会代码：ZGSP
• 会议名称：中国食品科学技术学会第十三届年会
• 会议地点：中国北京
• 主办单位：中国食品科学技术学会
• 学会名称：中国食品科学技术学会
• 页数：2
• 文件大小：112k
• 原文格式：O
• 会议级别：全国

摘要

臭鳜鱼是传统徽式名菜的代表,因其闻臭吃香而远近闻名。然而,其发酵体系中复杂的微生物群落结构给准确认识发酵过程、实现产品品质调控带来了巨大的挑战。本研究利用宏基因组学技术高保真、高通量的特点,结合传统分离鉴定,研究发酵鳜鱼中微生物的菌群结构,并对其安全性进行评价。首先,通过提取发酵完成时臭鳜鱼样品中的微生物总基因组,采用宏基因组测序技术进行分析;随后,采用传统分离纯化与16srD NA测序鉴定,对臭鳜鱼发酵体系的微生物进行分析。宏基因组测序结果表明,臭鳜鱼样品中主要优势菌群为F/sobacterium、Psychrilyobacter、Vibrio、Pseudoalteromonas、Photobacterium、Arcob∞ter,传统分离鉴定结果表明Poteus vulgaris、Morganella morganii、Obesumbacterium proteus、Aeromonas、Hafnia、Citrobacter可在臭鳜鱼样品中检测到,与宏基因组测序结果吻合;宏基因组测序结果与传统分离鉴定结果均表明,发酵臭鳜鱼中不含有水产品致病菌,因此该食品安全可靠,可放心食用。该研究结果为理性认识及调控臭鳜鱼发酵过程,进一步开发快速工艺提供了理论指导,为其产品品质控制提供了安全性基础。
Stinky mandarin fish is a representative of traditional Anhui dishes,and is famous for its special smells and tasty flavor.However,the complex microbial community ecosystem bringsgreat challenges to understand the fermentation process and control the product quality.In this study,we took advantages of the metagenomics,combining with the traditional isolation and identification method,to study microbial ecosystemand safety in the fermented mandarin fish system.Firstly,the total microbial genome was extracted after the fermentation was completed,then subjected to metagenomics analysis.Subsequently,the microorganism of stinky mandarin fish was isolated and purified by conventional method and underwent 16 S rDNA sequencing.Results of metagenomic sequencing indicated that Fusobacterium,Psychrilyobacter,Vibrio,Pseudoalteromonas,Photobacterium,and Arcobacter were the dominant bacterial community in stinky mandarin fish samples.The traditional isolation and identification results showedthat Proteus vulgaris,Morganella morganii,Obesumbacterium proteus,Aeromonas,Hafnia and Citrobacter couldbe detected in the system,which was consistent with thatof the metagenomics sequencing.These two methods both revealed that stinky mandarin fish didn't contain any pathogens of aquatic food,therefore,is safe to eat.This study provided knowledge to understand and control of the fermentation process.Further study of rapid fermentation development and quality control could be carried out in the future.

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