姬玉露不定芽诱导及高频离体再生体系的建立
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- 英文论文题名：Adventitious Buds Induction and Establishment of High Frequency in Vitro Regeneration System of Haworthia cooperi var.truncate
- 论文作者：任改婷 ; 张盛圣 ; 张桂芳 ; 李娜 ; 郭臣臣 ; 刘春 ; 张黎
- 英文论文作者：REN Gai-ting ; ZHANG Sheng-sheng ; ZHANG Gui-fang ; LI Na ; GUO Chen-chen ; LIU Chun ; ZHANG Li ; College of Agriculture ; Ningxia University ; Vegetable and Flower Research Institute ; Chinese Academy of Agricultural Sciences
- 年：2016
- 作者机构：宁夏大学农学院;中国农科院蔬菜花卉研究所;
- 论文关键词：姬玉露 ; 组织培养 ; 芽诱导
- 英文论文关键词：Haworthia cooperi var.truncate ; Tissue culture ; Buds induction
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.33
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：6
- 文件大小：1281k
- 原文格式：O
- 会议级别：全国
摘要
以姬玉露叶片为外植体,研究不同激素配比对愈伤组织诱导、再生和生根的影响,建立姬玉露高频离体再生体系。结果表明:愈伤诱导最佳配方MS+6-BA1.0mg/L+NAA0.1mg/L。芽诱导最佳培养基配方:MS+6-BA0.8mg/L+TDZ0.01mg/L+NAA0.3mg/L。增殖最佳培养基配方:MS+ZT0.8mg/L+NAA0.1mg/L,增殖系数达到10.04。生根最佳培养基配方:1/2 MS+NAA0.2mg/L。
With the leaf of Haworthia cooperi var.truncate as the explants,the influence of different ratio of hormones on the callus induction,regeneration and rooting was studied and a high frequency in vitro regeneration system was established.The results showed that:with the 0.1%HgCl2 and processing 8 min has the best effect and the rate of survival is 76.23%.The optimum medium for callus induction is MS + 6-BA1.0mg/L + NAA0.1mg/L The adventitious bud induction is MS + 6-BA0.8mg/L+ TDZ0.01mg/L + NAA0.3mg/L The medium for propagation of adventitious bud is MS + ZT0.8mg/L +NAA0.1mg/L,and the propagation factor is 10.04.The optimum for rooting is 1/2 MS + NAA0.2mg/L
With the leaf of Haworthia cooperi var.truncate as the explants,the influence of different ratio of hormones on the callus induction,regeneration and rooting was studied and a high frequency in vitro regeneration system was established.The results showed that:with the 0.1%HgCl2 and processing 8 min has the best effect and the rate of survival is 76.23%.The optimum medium for callus induction is MS + 6-BA1.0mg/L + NAA0.1mg/L The adventitious bud induction is MS + 6-BA0.8mg/L+ TDZ0.01mg/L + NAA0.3mg/L The medium for propagation of adventitious bud is MS + ZT0.8mg/L +NAA0.1mg/L,and the propagation factor is 10.04.The optimum for rooting is 1/2 MS + NAA0.2mg/L
引文
1.Beyl C.A.,and Sharma G.C.,1983,Picloram induced somatic embryogenesis in Gasteria and Haworhia,Plant Cell Tissue Organ Culture,2:123-132.
2.高越,王娅欣,孙涛,等.2010.毛玉露的组织培养与快速繁殖[J].生物学通报,45(6):54-55.
3.黄素华,肖惠匀,洪燕萍.水晶掌组织培养与快速繁殖[J].福建师范大学学报(自然科学版),2014,30(5):96-100.
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6.王紫珊,王广东,王雁.多肉植物白银寿‘奇迹'的离体培养与快速繁殖[J].基因组学与应用生物学,2014,33(06):1329-1335.
7.赵昂,左志宇,宋晓涛,等.Haworthia mirabilis的组织培养与快速繁殖[J].植物生理学通讯,2007,43(6):1137-1138.
2.高越,王娅欣,孙涛,等.2010.毛玉露的组织培养与快速繁殖[J].生物学通报,45(6):54-55.
3.黄素华,肖惠匀,洪燕萍.水晶掌组织培养与快速繁殖[J].福建师范大学学报(自然科学版),2014,30(5):96-100.
4.孙涛,金蕊,李德森.康平寿的组织培养与快速繁殖[J].植物生理学通讯,2003,39(3):232.
5.宋扬.冰灯玉露的组织培养与决速繁殖技术研究[J].现代农业科技,2014,(18):164-166.
6.王紫珊,王广东,王雁.多肉植物白银寿‘奇迹'的离体培养与快速繁殖[J].基因组学与应用生物学,2014,33(06):1329-1335.
7.赵昂,左志宇,宋晓涛,等.Haworthia mirabilis的组织培养与快速繁殖[J].植物生理学通讯,2007,43(6):1137-1138.