Missense mutations in the signal peptide of the porcine GH gene affect cellular synthesis and secretion

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• 英文论文题名：Missense mutations in the signal peptide of the porcine GH gene affect cellular synthesis and secretion
• 论文作者：Cheng yunyun ; Liu songcai ; Lu chao ; Wu qingyan ; Li siming ; Fu haoyu ; Wang gang ; Lv chen ; Nie linyan ; Zhang yu ; Yu hao ; Hao linlin
• 英文论文作者：Cheng yunyun ; Liu songcai ; Lu chao ; Wu qingyan ; Li siming ; Fu haoyu ; Wang gang ; Lv chen ; Nie linyan ; Zhang yu ; Yu hao ; Hao linlin ; College of Animal Science ; Jilin University ; Five-Star Animal Health Pharmaceutical Factory of Jilin Province
• 年：2016
• 作者机构：College of Animal Science,Jilin University;Five-Star Animal Health Pharmaceutical Factory of Jilin Province;
• 英文论文关键词：GH gene ; signal peptide ; cellular secretion ; RNA fold
• 会议召开时间：2016-05-25
• 会议录名称：第七届中国畜牧科技论坛论文集
• 英文会议录名称：Proceeding of the 7th China Animal Husbandry Sci-tech Forum
• 语种：英文
• 分类号：S828
• 学会代码：ZGXJ
• 会议名称：第七届中国畜牧科技论坛
• 会议地点：中国重庆
• 主办单位：中国畜牧兽医学会
• 学会名称：中国畜牧兽医学会
• 页数：1
• 文件大小：56k
• 原文格式：O
• 会议级别：全国

摘要

In previous investigations,we have demonstrated the mutations in the signal peptide of porcine GH gene were associated with the bodysize.In this study,the fusion gene expression vectors which consisted of eight signal peptide mutants of GH gene and EGFP gene were constructed according to three missense mutations(p.Val9 Ala,p.Gln22 Arg and p.Asp25Gly),and they were transfected into the GH3 cell line.The inhibition levels of EGFP gene transcriptions with different signal peptide mutants were significantly different.Typically,the allelic variants carrying Val in codon 9 showed higher protein synthesis(P<0.05),and the allelic variants carrying neutral Gin in codon 22 and and Gly in codon 25 showed higher secretion proportion(P<0.05) compared with the other groups as assessed by western blotting.In silico RNA folding prediction indicated that the mutations gave rise to different RNA secondary structures,suggesting that they might affect translation and protein synthesis.We conclude that the missense mutations within the signal sequence influence the expression and the secretion of the protein.To the best of our knowledge,this is the first report addressing the functional consequences of the mutations in the signal peptide of porcine GH gene.
In previous investigations,we have demonstrated the mutations in the signal peptide of porcine GH gene were associated with the bodysize.In this study,the fusion gene expression vectors which consisted of eight signal peptide mutants of GH gene and EGFP gene were constructed according to three missense mutations(p.Val9 Ala,p.Gln22 Arg and p.Asp25Gly),and they were transfected into the GH3 cell line.The inhibition levels of EGFP gene transcriptions with different signal peptide mutants were significantly different.Typically,the allelic variants carrying Val in codon 9 showed higher protein synthesis(P<0.05),and the allelic variants carrying neutral Gin in codon 22 and and Gly in codon 25 showed higher secretion proportion(P<0.05) compared with the other groups as assessed by western blotting.In silico RNA folding prediction indicated that the mutations gave rise to different RNA secondary structures,suggesting that they might affect translation and protein synthesis.We conclude that the missense mutations within the signal sequence influence the expression and the secretion of the protein.To the best of our knowledge,this is the first report addressing the functional consequences of the mutations in the signal peptide of porcine GH gene.

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