Study on the molecular mechanism of trichloroethylene-induced allergic dermatitis

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• 英文论文题名：Study on the molecular mechanism of trichloroethylene-induced allergic dermatitis
• 论文作者：徐新云 ; 刘月峰 ; 李学余 ; Xiaoyun Qin ; Kanlang Mao
• 英文论文作者：Xinyun Xu ; Yuefeng Liu ; Xueyu Li ; Xiaoyun Qin ; Kanlang Mao ; Department of toxicology ; Shenzhen center for disease control and prevention
• 年：2016
• 作者机构：深圳市疾病预防控制中心毒理学重点实验室;
• 英文论文关键词：Trichloroethylene ; allergic dermatitis ; liver dysfunction ; hepatic metabolic enzyme ; immune-related genes ; oncogene
• 会议召开时间：2016-11-16
• 会议录名称：第六届中国毒理学会免疫毒理专业委员会换届及学术会议资料汇编
• 语种：英文
• 分类号：R135.7
• 学会代码：ZGDV
• 会议名称：第六届中国毒理学会免疫毒理专业委员会换届及学术会议
• 会议地点：中国河南开封
• 主办单位：中国毒理学会免疫毒理专业委员会
• 学会名称：中国毒理学会
• 页数：2
• 文件大小：143k
• 原文格式：O
• 会议级别：全国

摘要

Trichloroethylene(TCE) is one of the common organic solvents that has been widely used in cleaning or degreasing of metal products and electronic products.However,hundred cases of hypersensitivity dermatitis have occurred after the workers were occupationally exposed to TCE in China for the past decad.The purpose of this study was to investigate mRNA expression of hepatic metabolic enzyme genes,immune-related genes,apoptosis genes and oncogenes in patients with allergic dermatitis induced by trichloroethylene.12 typical patients with TCE-induced hypersensitivity dermatitis were investigated as the study cases,peripheral blood samples were taken from patients and control,real-time fluorescent PCR assay was applied for detection of mRNA expression of hepatic metabolic enzyme genes,immune-related genes,apoptosis genes and oncogenes.It was found that the relative levels of mRNA expression of CYP1A2,CYP2E1,CYP3A4,CYP2C9 increased by723%,318%,385%,216%,respectively,when compared with control(P<0.01 or P<0.05);Foxp3,GATA3 and CTLA4 mRNA expression increased by 104%,106%,253%,respectively,in TCE patients when compared with control(P<0.01);T-bet expression decreased by 44%when compared with control(P<0.01);These findings indicate that some immune-related genes and hepatic metabolic enzyme genes might play an important role in the process of trichloroethylene-induced allergic dermatitis.
Trichloroethylene(TCE) is one of the common organic solvents that has been widely used in cleaning or degreasing of metal products and electronic products.However,hundred cases of hypersensitivity dermatitis have occurred after the workers were occupationally exposed to TCE in China for the past decad.The purpose of this study was to investigate mRNA expression of hepatic metabolic enzyme genes,immune-related genes,apoptosis genes and oncogenes in patients with allergic dermatitis induced by trichloroethylene.12 typical patients with TCE-induced hypersensitivity dermatitis were investigated as the study cases,peripheral blood samples were taken from patients and control,real-time fluorescent PCR assay was applied for detection of mRNA expression of hepatic metabolic enzyme genes,immune-related genes,apoptosis genes and oncogenes.It was found that the relative levels of mRNA expression of CYP1A2,CYP2E1,CYP3A4,CYP2C9 increased by723%,318%,385%,216%,respectively,when compared with control(P<0.01 or P<0.05);Foxp3,GATA3 and CTLA4 mRNA expression increased by 104%,106%,253%,respectively,in TCE patients when compared with control(P<0.01);T-bet expression decreased by 44%when compared with control(P<0.01);These findings indicate that some immune-related genes and hepatic metabolic enzyme genes might play an important role in the process of trichloroethylene-induced allergic dermatitis.

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