产玫瑰红色素粘质沙雷氏菌的分离鉴定及抑菌活性研究

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• 英文论文题名：Isolation,identification and antibacterial activities of Serratia marcescens producing rosy pigment
• 论文作者：董婷 ; 景波 ; 李伟 ; 黄晓雅 ; 钱俏君 ; 杨慧 ; 潘康成
• 英文论文作者：DONG Ting ; JING Bo ; LI Wei ; Huang Xiao-ya ; QIAN Qiao-jun ; YANG Hui ; PAN Kang-cheng ; Research Center of Animal Microecology ; College of Veterinary Medicine ; Sichuan Agricultural University ; Key Laboratory of Animal Disease and Human Health of Sichuan Province
• 年：2016
• 作者机构：四川农业大学动物微生态研究中心;动物疫病与人类健康四川省重点实验室;
• 论文关键词：粘质沙雷氏菌 ; 分离 ; 鉴定 ; 灵菌红素 ; 生物拮抗
• 英文论文关键词：Serratia marcescens ; isolation ; identification ; Prodigiosin ; biological antagonism
• 会议召开时间：2016-11-04
• 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
• 语种：中文
• 分类号：Q936
• 学会代码：ZGXJ
• 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
• 会议地点：中国山东青岛
• 主办单位：中国畜牧兽医学会动物微生态学分会
• 学会名称：中国畜牧兽医学会
• 页数：1
• 文件大小：81k
• 原文格式：O
• 会议级别：全国

摘要

本研究旨在分离鉴定产玫瑰红色素菌株,及其分离菌株和色素对病原菌的生物拮抗作用。经形态观察、生理生化试验和16S rRNA序列分析对其进行鉴定,通过控制变量法确定最佳产色素的温度及光照条件,破裂菌体提取色素分析此菌株产色素的量和红色素的性质,采用滤纸片法研究对病原菌的拮抗作用。结果表明:(1)分离细菌Dse-01菌株最终鉴定为粘质沙雷氏菌(Serratia marcescens);(2)该菌在25~35℃条件下培养色素形成较早且颜色较深,30℃温度下产色素最佳,光照对其色素形成时间没有明显影响;(3)30℃150 r·min~(-1)培养24 h,提取获得色素粗品确定为灵菌红素,产量达567.9±7.77 mg·L~(-1);(4)分离菌株的全菌液、上清液、菌体和提取色素对病原性大肠杆菌、沙门氏菌均无拮抗作用,而全菌液、菌体和提取色素对金黄色葡萄球菌具有明显的抑制作用。
This experiment was conducted to isolate and identify a microbial strain producing rosy pigment,and to study its antibacterial activity.The microbial strains were identified by morphological characteristics,physiochemical properties and 16 S rRNA gene sequence analyzation.The optimization of temperature and illumination condition for rosy pigment production was investigated by Variable-controlling approach.Ultrasonic fragmentation was used to extract pigment Secreta and analysis its yield and absorption spectrum.In eventually,antagonism assay to pathogens also be carried out by filter-paper method.The results showed that:(1) a screened strain Dse-01 was showed completely identity with Serratia marcescens;(2) there were significant impact on chromogenesis under the condition of 25℃~35℃,especially in 30℃;parameters were not affected by illuminance administration;(3) a strain cultured at 30℃ for 24 h with a shaking speed of 150 r·min~(-1),the production of native prodigiosin could reach to 567.9±7.77 mg·L~(-1);(4) the pathogenic Escherichia coli and Salmonella spp.were resistance to four active substance:Serratia marcescens cultures,sediment of Serratia marcescens cultures,supernatant of Serratia marcescens cultures and pigment extraction from Serratia marcescens,whereas,Staphylococcus aureus was sensitive to all of these active products besides supernatant.

引文

[1]吴琦,代剑渡,容杰,等.一株温敏型产红色素粘质沙雷氏菌的分离及其培养条件研究[J].中国酿造,2009,(09):51-54.
    [2]Pandey R,Chander R,Sainis KB.A novel prodigiosin-like immunosuppressant from an alkalophilic Micrococcus sp.[J].International Immunopharmacology,2003,3(2):159-167.
    [3]Boric M,Danevcic T,Stopar D.Prodigiosin from Vibrio sp DSM 14379;A new UV-protective pigment[J].Microbial Ecology,2011,62(3):528-536.