甘肃甘南野生羊肚菌遗传多样性研究
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- 英文论文题名：Study on Genetic Diversity of Wild Morchella spp.from Gannan in Gansu Province
- 论文作者：王龙 ; 秦鹏 ; 郭瑞 ; 路等学 ; 赵玉卉
- 英文论文作者：WANG Long ; QIN Peng ; GUO Rui ; LU Deng-xue ; ZHAO Yu-hui ; Institute of Biology ; Gansu Academy of Science/Key Laboratory of Microbial Resources Development and Utilization of Gansu Province ; Industrial Microbial Engineering Technology Research Center of Gansu Province
- 年：2016
- 作者机构：甘肃省科学院生物研究所/甘肃省微生物资源开发利用重点实验室/甘肃省工业微生物工程技术研究中心;
- 论文关键词：羊肚菌 ; 分子系统学 ; ITS序列分析 ; 甘南藏族自治州
- 英文论文关键词：Morchella spp ; molecular systematics ; ITS sequence analysis ; Tibetan Autonomous Prefecture of Gannan
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：中文
- 分类号：S646.7
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：2
- 文件大小：808k
- 原文格式：D
- 会议级别：全国
摘要
羊肚菌(Morchella spp.)是一类珍稀食用和药用真菌,具有重要的经济和科研价值。由于该物种形态特征具有高度的多变性,仅靠传统的形态学特征基本无法准确辨别。本文基于自2012年~2015年间赴甘肃甘南境内采收到的16份野生羊肚菌标本进行ITS基因标记筛选,研究发现16份供试材料共归纳为5种,分别为黑脉羊肚菌(M.angusticeps),羊肚菌(M.esculenta),高羊肚菌(M.elata),粗腿羊肚菌(M.crapssipes)和尖顶羊肚菌(M.conica)。此外,基于最大简约法(Maximum Parsimony)和邻接法(Neighbor Joining)构建的分子进化树得知,这5种羊肚菌主要归类于黄羊肚菌类群(Yellow morels group)和黑羊肚菌类群(Black morels group)。
As one group of the world's most prized edible and medicinal fungi,Morchella spp.are very important economic and scientific values.Because the morphological characteristics of these species has a high variability,it is unable to accurately identify morphological features.according to traditional method.In this paper,16 strains of wild Morchella spp.which collected in Gannan of Gansu province from 2012 to 2015 were researched based on ITS sequences.The result showed that 16 strains of wild Morchella spp.were classified 5 species.There were M.angusticeps,M.esculenta,M.elata,M.crapssipes,M.conica.In addition,according to the molecular phylogenetic trees which were constructed by Maximum parsimony method & Neighbor joining method.These kinds of 5 species were respectively belonged to yellow morels group & black morels group.
As one group of the world's most prized edible and medicinal fungi,Morchella spp.are very important economic and scientific values.Because the morphological characteristics of these species has a high variability,it is unable to accurately identify morphological features.according to traditional method.In this paper,16 strains of wild Morchella spp.which collected in Gannan of Gansu province from 2012 to 2015 were researched based on ITS sequences.The result showed that 16 strains of wild Morchella spp.were classified 5 species.There were M.angusticeps,M.esculenta,M.elata,M.crapssipes,M.conica.In addition,according to the molecular phylogenetic trees which were constructed by Maximum parsimony method & Neighbor joining method.These kinds of 5 species were respectively belonged to yellow morels group & black morels group.
引文
[1]黄年来,林志彬,陈国良.中国食药用菌学[M].上海:上海科学技术文献出版社,2010:1375-1391.
[2]黄年来.中国大型真菌原色图鉴[M].北京:中国农业出版社,1998:473-512.
[3]李娟,王臻,姚良同,贾乐.羊肚菌多糖研究进展[J].微生物学杂志,2005,25(04):89-91.
[4]周丽伟.羊肚菌培养条件优化及其多糖生物活性的研究[D].合肥:安徽大学,2007.
[5]任桂梅,张少刚.羊肚菌的研究进展[J].延安大学学报(自然科学版),1999,18(01):57-64.
[6]Emile J..Les Morilles[M].Paris:La Bibliotheque des Arts paris,1984:114p.
[7]Kirk P.M.,Cannon P.F.,David J.C.,Ainsworth and Bisby.Dictionary of the fungi[M].9thed.London:CABinternational Wallingford,2001:Ⅺ+655p.
[8]谢占玲,谢占青.羊肚菌研究综述[J].青海大学学报(自然科学版),2007,25(02):36-40.
[9]顾龙云.甘南山珍-羊肚菌[J].食用菌,1983,5(04):1-2.
[10]罗植柚.甘南野生羊肚菌生态研究初报[J].中国食用菌.1986,8(01):30-32.
[11]白永宏,陈国梁,闫冬,张向前.几种食用菌子实体总DNA提取方法的比较研究[J].安徽农业科学,2011,39(19):11409-11410.
[12]White T J,Bruns T,Lee S.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M].New York:Academic Press,1990:315-322.
[13]戴璐,李峻志,李安利,祁鹏,丁仕刚.基于ITS序列分析对秦岭冷水沟羊肚菌的鉴定[J].中国食用菌,2010,29(06):45-46.
[14]刘文丛,刘颖,郭相,刘弟,马明,李宗菊,马绍宾.滇西北地区羊肚菌的分子鉴定及ITS序列分析[J].江苏农业科学,2012,40(03):31-34.
[15]Chenna R,Sugawara H,Koike T.Multiple sequence alignment with the Clustal series of programs[J].Nucleic Acids Research,2003,31(13):3497-3500.
[16]包英华,白音.ITS序列在被子植物系统学上的应用[J].内蒙古科技与经济,2000,4(S1):442+358.
[17]和晓娜.秦岭中东段地区羊肚菌的分类鉴定及生物学特性研究[D].西安:陕西师范大学.2012.
[18]Bunyard B.A.,Nicholson M.S.and Royse D.J.A systematic assessment of Morchella using RFLP analysis of the 28S ribosomal gene[J].Mycological Research.1994,86(6):762-772.
[19]Harpreet Kaur Kanwal,Karan Acharya,G.Ramesh,M.Sudhakara Reddy.Molecular Charact-erization of Mor chella Species from the Western Himalayan Region of India[J].Current Microbiology,2011,62(4):1245-1252.
[20]Moncalvo J M,Wang H H,Hseu R S.Phylogenetic relationship in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences[J].Mycologia,1987,79(2):223-238.
[21]詹才新,凌霞芬,杨家林.双孢蘑菇性亲和性相关分子标记的初步筛选[J].食用菌学报,2002,9(4):1-8..
[22]罗信昌,李英波,施庆利.分子生物学技术在菇类研究中的应用[J].食用菌学报,1995,2(4):1-10..
[23]李荣春,张丽梅.随机扩增多态DNA(RAPD)在野蘑菇杂交育种中的应用[J].微生物学通报,2001,28(5):52-55.
[24]Ramirez L,Muez V and Alfonso M.Use of molecular markers to differentiate between commercial strains of the button mushoom Agaricus bisporus[J].FEMS Microbiol Lett,2001,198(01):45-48.
[25]马富英,罗信昌.28Sr DNA PCR-RFLP分析在侧耳属系统发育研究中的应用[J].华中农业大学学报,2002,21(3):201-205.
[2]黄年来.中国大型真菌原色图鉴[M].北京:中国农业出版社,1998:473-512.
[3]李娟,王臻,姚良同,贾乐.羊肚菌多糖研究进展[J].微生物学杂志,2005,25(04):89-91.
[4]周丽伟.羊肚菌培养条件优化及其多糖生物活性的研究[D].合肥:安徽大学,2007.
[5]任桂梅,张少刚.羊肚菌的研究进展[J].延安大学学报(自然科学版),1999,18(01):57-64.
[6]Emile J..Les Morilles[M].Paris:La Bibliotheque des Arts paris,1984:114p.
[7]Kirk P.M.,Cannon P.F.,David J.C.,Ainsworth and Bisby.Dictionary of the fungi[M].9thed.London:CABinternational Wallingford,2001:Ⅺ+655p.
[8]谢占玲,谢占青.羊肚菌研究综述[J].青海大学学报(自然科学版),2007,25(02):36-40.
[9]顾龙云.甘南山珍-羊肚菌[J].食用菌,1983,5(04):1-2.
[10]罗植柚.甘南野生羊肚菌生态研究初报[J].中国食用菌.1986,8(01):30-32.
[11]白永宏,陈国梁,闫冬,张向前.几种食用菌子实体总DNA提取方法的比较研究[J].安徽农业科学,2011,39(19):11409-11410.
[12]White T J,Bruns T,Lee S.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M].New York:Academic Press,1990:315-322.
[13]戴璐,李峻志,李安利,祁鹏,丁仕刚.基于ITS序列分析对秦岭冷水沟羊肚菌的鉴定[J].中国食用菌,2010,29(06):45-46.
[14]刘文丛,刘颖,郭相,刘弟,马明,李宗菊,马绍宾.滇西北地区羊肚菌的分子鉴定及ITS序列分析[J].江苏农业科学,2012,40(03):31-34.
[15]Chenna R,Sugawara H,Koike T.Multiple sequence alignment with the Clustal series of programs[J].Nucleic Acids Research,2003,31(13):3497-3500.
[16]包英华,白音.ITS序列在被子植物系统学上的应用[J].内蒙古科技与经济,2000,4(S1):442+358.
[17]和晓娜.秦岭中东段地区羊肚菌的分类鉴定及生物学特性研究[D].西安:陕西师范大学.2012.
[18]Bunyard B.A.,Nicholson M.S.and Royse D.J.A systematic assessment of Morchella using RFLP analysis of the 28S ribosomal gene[J].Mycological Research.1994,86(6):762-772.
[19]Harpreet Kaur Kanwal,Karan Acharya,G.Ramesh,M.Sudhakara Reddy.Molecular Charact-erization of Mor chella Species from the Western Himalayan Region of India[J].Current Microbiology,2011,62(4):1245-1252.
[20]Moncalvo J M,Wang H H,Hseu R S.Phylogenetic relationship in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences[J].Mycologia,1987,79(2):223-238.
[21]詹才新,凌霞芬,杨家林.双孢蘑菇性亲和性相关分子标记的初步筛选[J].食用菌学报,2002,9(4):1-8..
[22]罗信昌,李英波,施庆利.分子生物学技术在菇类研究中的应用[J].食用菌学报,1995,2(4):1-10..
[23]李荣春,张丽梅.随机扩增多态DNA(RAPD)在野蘑菇杂交育种中的应用[J].微生物学通报,2001,28(5):52-55.
[24]Ramirez L,Muez V and Alfonso M.Use of molecular markers to differentiate between commercial strains of the button mushoom Agaricus bisporus[J].FEMS Microbiol Lett,2001,198(01):45-48.
[25]马富英,罗信昌.28Sr DNA PCR-RFLP分析在侧耳属系统发育研究中的应用[J].华中农业大学学报,2002,21(3):201-205.
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