猴源宋内氏志贺氏菌的分离鉴定及生物学特性分析

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• 英文论文题名：Isolation,identification and biological characteristics of Shigella sonnei from rhesus macaques(Macaca mulatta)
• 论文作者：邹瑶 ; 张露 ; 陈正礼 ; 黄超 ; 刘文涛 ; 罗启慧 ; 徐婧 ; 夏玉 ; 阳丹丹
• 英文论文作者：ZOU Yao ; ZHANG Lu ; CHEN Zheng-li ; HUANG Chao ; LIU Wen-tao ; LUO Qi-hui ; XU Jing ; XIA Yu ; YANG Dan-dan ; Key Laboratory of Animal Disease and Human Health of Sichuan Province ; College of Veterinary Medicine ; Sichuan Agricultural University ; Laboratory of Animal Disease Model ; College of Veterinary Medicine ; Sichuan Agricultural University
• 年：2016
• 作者机构：四川农业大学动物医学院实验动物疾病模型研究室;四川农业大学动物医学院动物疾病与人类健康中心四川省重点实验室;
• 论文关键词：猕猴 ; 痢疾 ; 宋内氏志贺氏菌 ; 分离鉴定 ; 16Sr RNA ; 药敏试验
• 英文论文关键词：Rhesus macaques/Macaca mulatta ; Diarrhea ; Shigella sonnei ; 16S rRNA ; Isolation and identification ; Antimicrobial susceptibility testing
• 会议召开时间：2016-11-04
• 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
• 语种：中文
• 分类号：S858.93
• 学会代码：ZGXJ
• 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
• 会议地点：中国山东青岛
• 主办单位：中国畜牧兽医学会动物微生态学分会
• 学会名称：中国畜牧兽医学会
• 页数：1
• 文件大小：72k
• 原文格式：O
• 会议级别：全国

摘要

从四川某规模化猕猴养殖基地病死猕猴脏器及肠道内容物中分离到1株可疑菌,对其纯化培养、革兰氏染色和生化鉴定后,进行了分子水平鉴定包括ipaH基因和wzy基因测定、16SrRNA基因序列分析,并进行了致病性分析及药敏试验。结果显示,该分离菌符合宋内氏志贺氏菌生物学及分子学特性,对小鼠具有较强致病性。16Sr RNA基因多序列比对,绘制遗传距离及遗传发育树,证明其与两株宋内氏志贺氏菌(CP010829.1和HE616528.1)遗传距离最小,均为0.001。药敏试验显示,该菌株对头孢类等4类药物敏感,对青霉素类等6类药物耐药。结论:该分离株为宋内氏志贺氏菌,并且具有较强的致病性和耐药性。
A strain of bacterium was isolated from stool specimens of diarrheal rhesus macaques(Macaca mulatta) in a large-scale farm located in Sichuan,China.The procedures of microbiological tests,including culture and purification,Gram-stain,biochemical tests,PCR-based detection of ipaH and wzy gene,pathogenicity test,antimicrobial susceptibility testing and 16 S rRNA gene sequence analysis,were used for isolation and identification of pathogen.The results demonstrate ipaH and wzy are positive,and the biochemical and biological features of the isolate correspond to Shigella sonnei's.The isolate has strong pathogenicity for mice.Compared with others,the genetic distances of 16 S rRNA gene between the isolate and two strains of Shigella sonnei(sequence ID:CPO 10829.1 and HE616528.1) are minimal and both 0.001.Furthermore,the isolate is sensitive to four kinds of antimicrobial including cephems,and is resistant to six kinds of antimicrobial including penicillins.In conclusion,the isolate is identified to be Shigella sonnei and displays strong pathogenicity and antimicrobial drug resistance.

引文

[1]DUPONT H L,LEVINE M M,HOMICK R B,et al.Inoculum size in shigellosis and implications for expected mode of transmission[J].J Infect Dis,1989,159(6):1126-1128.