凡纳滨对虾急性肝胰腺坏死综合征(AHPND)拮抗菌的筛选和效果评价

详细信息    查看官网全文

• 英文论文题名：Screening and evaluation of potential probiotics against AHPND in Litopenaenaeus vannamei
• 论文作者：唐杨 ; 王春迪 ; 宋晓玲 ; 万晓媛 ; 黄捷
• 英文论文作者：Tang Yang ; WangChun-di ; SongXiao-ling ; WanXiao-yuan ; Huang Jie ; Yellow Sea Fisheries Research Institute ; Chinese Academy of Fishery Sciences ; Shanghai Ocean University ; Function Laboratory for Marine Fisheries Science and Food Production Processes ; Qingdao National Laboratory for Marine Science and Technology
• 年：2016
• 作者机构：中国水产科学研究院黄海水产研究所;上海海洋大学水产与生命学院;青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室;
• 论文关键词：分离 ; 副溶血弧菌 ; 益生菌 ; 凡纳滨对虾 ; 胞外产物
• 英文论文关键词：isolation ; VPAHPND ; probiotic ; Litopenaenaeus vannamei ; ECPs
• 会议召开时间：2016-11-02
• 会议录名称：2016年中国水产学会学术年会论文摘要集
• 语种：中文
• 分类号：S945.49
• 学会代码：OGSB
• 会议名称：2016年中国水产学会学术年会
• 会议地点：中国四川成都
• 主办单位：中国水产学会、四川省水产学会
• 学会名称：中国水产学会
• 页数：1
• 文件大小：563k
• 原文格式：D
• 会议级别：全国

摘要

本实验以可以引起急性肝胰腺坏死综合征(AHPND)的副溶血弧菌为指示菌,从对虾肠道中分离出两株拮抗菌,基于生化特性以及16srDNA序列,m8和a22与假交替单胞菌同源性相近。在对两株拮抗菌胞外产物实验中发现,过氧化氢酶能够使ECPs的抑菌活性消失,并且当胞外产物中加入L-Lys时过氧化氢的产生量明显高于不加入氨基酸的胞外产物。养殖实验中,实验组A和B的对虾肠道弧菌数量明显低于空白对照组D。经过28天的饲养后,进行副溶血弧菌攻毒,饲料中添加m8和a22的累积死亡率分别是30.0%±0.18,68.3%±0.03,显著低于空白对照组的95.0%±0.15。在毒力基因定量研究中,qPCR结果显示对虾鳃组织中弧菌毒力基因的拷贝数在8.43±3.10×10~1到6.63±0.83×10~3之间。
Two bacterial strains were isolated from the gut of shrimps and screened for antagonistic activity against Vibrio parahaemolyticus, which results in AHPND. The putative probiotic strains m8 and a22 were identified as Pseudoalteromonas spp. based on their biochemical properties and 16 S rRNA gene sequencing. The antibacterial activity of extracellular products(ECPs) of m8 and a22 could be abolished in the presence of catalase, the production of hydrogen peroxide derived from the mixture of ECPs with l-Lys was significantly higher than that of the ECPs only. The Vibrios counts in the intestinal tracts of the two treated groups(A and B)were significantly lower than in the control group(D). After a 28-day feeding trial, the shrimps were challenged with VPAHPND. The cumulative mortalities of shrimps fed with m8 or a22 only were 30.0%±0.18 and 68.3%±0.03, respectively, significantly lower than the control group, which had a cumulative mortality of 95.0%±0.15.

引文