表达禽流感病毒H7亚型HA基因的禽痘重组病毒的构建及其免疫效力的评估
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摘要
禽流感(Avian Influenza,AI)是由A型流感病毒引起的禽类的感染和/或疾病综合征,广泛流行于世界上许多国家和地区,给养禽业造成了巨大的经济损失。高致病力禽流感(HPAI)多以突然发病和高死亡率为主要特征,常常导致感染鸡群的全群覆没,被国际兽疫局列为A类烈性传染病。近几年来,在我国的一些地区也相继出现了AI的流行, 1997香港禽流感直接传播给人并致人死亡事件的发生,更加突出了AIV的公共卫生学意义。目前,H5亚型高致病性禽流感成为研究的焦点,而H7亚型高致病性禽流感的研究报道较少,其相关的生物制品比较匮乏。虽然近年来H7型未出现大规模的流行,但禽流感不同亚型的流行变幻莫测,本研究构建的H7亚型重组禽痘活载体疫苗将为我国H7亚型高致病性禽流感的防治提供物质储备和技术支持。
本研究将我国H7亚型禽流感分离株A/Chicken/Hebei/1/02(H7N2)的HA基因克隆到pSY538质粒上,把含有禽痘病毒启动子P11的LacZ基因克隆到质粒pSY538-HA的SmaI位点,然后切下同时含有HA和LacZ基因的片段再克隆到禽痘病毒载体pSY681的NotI位点,构建出禽痘病毒转移载pSY681-HA7-LacZ。将重组质粒与亲本禽痘病毒S-FPV-017共转染鸡胚成纤维细胞,进行同源重组,通过加入X-gal进行阳性重组病毒的筛选、纯化,获得了遗传性状稳定的表达H7亚型HA基因的重组禽痘病毒rFPV- H7HA。提取重组病毒的总DNA进行PCR扩增,获得了1.7kb的携带有外源目的基因片段,证明所获得的重组病毒携带有外源目的基因片段;Western blot检测结果表明:重组痘病毒能在体外的CEF细胞有效表达HA糖蛋白。
分别用106、104、102的PFU剂量的重组病毒rFPV- H7HB免疫4周龄SPF鸡,4周后分别用100LD50的HPAIV A/FPV/Rostock/34 (H7N1)鼻腔途径进行攻击,观察发病与死亡情况,分别于攻毒后第3、5、7天采集喉头及泄殖腔拭子进行病毒分离、滴定检测排毒情况,同时检测免疫后、攻毒前及攻毒后血清HI抗体、AGP抗体的动态变化,对重组病毒的免疫保护效果进行全面评估。结果,不同剂量的重组病毒免疫后均可诱导出较高水平的HI抗体,抗体效价在免疫后可达到6lg2,而且其AGP抗体均为阴性;在病毒攻击后免疫鸡无一发病和死亡,攻毒保护率为100%,病毒分离和滴定结果显示所有免疫鸡没有发生排毒;而对照组全部发病并在攻毒后3-8天相继死亡,发病率和死亡率均为100%,并在其泻殖腔和喉头拭子中均分离到了较高滴度的病毒。本试验研究结果表明,稳定高效表达我国H7亚型禽流感分离株A/Chicken/Hebei/1/02(H7N2)HA基因的重组禽痘病毒rFPV- H7HA能有效诱导机体的体液免疫反应,可以对H7亚型高致病性病毒的攻击产生100%的免疫保护,此重组活载体疫苗可为我国H7亚型高致病性禽流感的防制提供必要和有效的物质技术储备。
Avian influenza(AI)is an infection and/or disease syndrome caused by type A influenza virus. It is widespread in many countries and areas throughout the world, and of great economic losses in poultry industry. The great characteristic of the highly pathogenic avian influenza (HPAI) is high mortality, and it is classified A virulent infectious disease by O.I.E. In China,.H5N1 subtype of AIV infected human directly, especially caused them dead, which was the first time that AIV overacrossed interspecies barrier and infected human. H5 subtypes highly pathogenic avian influenza virus (HPAIV) becomes the big issue of the current research. There are a few of reports about H7 subtype HPAIV and have not enough biology products referred to them. Although the H7subtype have not widespread in recently years, we can’t forecast which substype of HPAVI will widespread. The H7 subtype recombinant fowlpox virus constructed in this study would provide the storage of vaccine and technology for preventing H7 subtype HPAI.
In this study,the HA gene of A/Chicken/Hebei/1/02(H7N2) which was the only isolation from China was cloned into vector pSY538.Then the fragment LacZ which includes promoter P11 was cloned into the SmalI site in pSY538.At last, the fragment consist of HA gene and LacZ gene was sub-cloned into the NotI site in vector pSY681 and successfully generated the transferring vector pSY681-HA7-LacZ. Chick embryo fibroblast cell (CEF) was co-transfected with plasmid pSY681-HA7-LacZ and s-FPV-017 virus for homologous recombinant. Recombinant virus rFPV- H7HA was screened in the presence of X-gal to purify positive recombinant virus. The genome DNA of the recombinant fowl pox virus was used as template for PCR and 1.7 Kb HA gene fragments was obtained.This result indicated that the target gene was inserted into the genome DNA successfully. The results of Western-blot demonstrated that hemagglutinin glycoprotein of AIV was expressed efficiently in CEF infected with the recombinant pox virus in vitro.
Four- week–old SPF chickens were immunized with different doses of 106、104、102 PFU rFPV- H7HA and challenged with 100LD50 of HPAIV A/FPV/Rostock/34 (H7N1) through nostril .The throat and cloacal chamber swabs were collected 3、5、7 days after challenging. And chickens were observed daily for disease signs and deaths for 2 weeks. Sera were collected every week after vaccination for detecting the HI and AGP antibodies. Results shown that all chickens vaccinated with different doses of rFPV-H7HB generated high HI antibodies and were completely protected from virus challenge (no disease signs, no virus shedding and no deaths), but the control group died within eight days and can be detected high titers of H7 HPAIV from throat and cloacal chamber swabs. The results implied that the recombinant virus rFPV-H7HA that expressing HA gene of A/Chicken/Hebei/1/02(H7N2) would be the storage of vaccine and technology for preventing H7 subtypes HPAIV.
本研究将我国H7亚型禽流感分离株A/Chicken/Hebei/1/02(H7N2)的HA基因克隆到pSY538质粒上,把含有禽痘病毒启动子P11的LacZ基因克隆到质粒pSY538-HA的SmaI位点,然后切下同时含有HA和LacZ基因的片段再克隆到禽痘病毒载体pSY681的NotI位点,构建出禽痘病毒转移载pSY681-HA7-LacZ。将重组质粒与亲本禽痘病毒S-FPV-017共转染鸡胚成纤维细胞,进行同源重组,通过加入X-gal进行阳性重组病毒的筛选、纯化,获得了遗传性状稳定的表达H7亚型HA基因的重组禽痘病毒rFPV- H7HA。提取重组病毒的总DNA进行PCR扩增,获得了1.7kb的携带有外源目的基因片段,证明所获得的重组病毒携带有外源目的基因片段;Western blot检测结果表明:重组痘病毒能在体外的CEF细胞有效表达HA糖蛋白。
分别用106、104、102的PFU剂量的重组病毒rFPV- H7HB免疫4周龄SPF鸡,4周后分别用100LD50的HPAIV A/FPV/Rostock/34 (H7N1)鼻腔途径进行攻击,观察发病与死亡情况,分别于攻毒后第3、5、7天采集喉头及泄殖腔拭子进行病毒分离、滴定检测排毒情况,同时检测免疫后、攻毒前及攻毒后血清HI抗体、AGP抗体的动态变化,对重组病毒的免疫保护效果进行全面评估。结果,不同剂量的重组病毒免疫后均可诱导出较高水平的HI抗体,抗体效价在免疫后可达到6lg2,而且其AGP抗体均为阴性;在病毒攻击后免疫鸡无一发病和死亡,攻毒保护率为100%,病毒分离和滴定结果显示所有免疫鸡没有发生排毒;而对照组全部发病并在攻毒后3-8天相继死亡,发病率和死亡率均为100%,并在其泻殖腔和喉头拭子中均分离到了较高滴度的病毒。本试验研究结果表明,稳定高效表达我国H7亚型禽流感分离株A/Chicken/Hebei/1/02(H7N2)HA基因的重组禽痘病毒rFPV- H7HA能有效诱导机体的体液免疫反应,可以对H7亚型高致病性病毒的攻击产生100%的免疫保护,此重组活载体疫苗可为我国H7亚型高致病性禽流感的防制提供必要和有效的物质技术储备。
Avian influenza(AI)is an infection and/or disease syndrome caused by type A influenza virus. It is widespread in many countries and areas throughout the world, and of great economic losses in poultry industry. The great characteristic of the highly pathogenic avian influenza (HPAI) is high mortality, and it is classified A virulent infectious disease by O.I.E. In China,.H5N1 subtype of AIV infected human directly, especially caused them dead, which was the first time that AIV overacrossed interspecies barrier and infected human. H5 subtypes highly pathogenic avian influenza virus (HPAIV) becomes the big issue of the current research. There are a few of reports about H7 subtype HPAIV and have not enough biology products referred to them. Although the H7subtype have not widespread in recently years, we can’t forecast which substype of HPAVI will widespread. The H7 subtype recombinant fowlpox virus constructed in this study would provide the storage of vaccine and technology for preventing H7 subtype HPAI.
In this study,the HA gene of A/Chicken/Hebei/1/02(H7N2) which was the only isolation from China was cloned into vector pSY538.Then the fragment LacZ which includes promoter P11 was cloned into the SmalI site in pSY538.At last, the fragment consist of HA gene and LacZ gene was sub-cloned into the NotI site in vector pSY681 and successfully generated the transferring vector pSY681-HA7-LacZ. Chick embryo fibroblast cell (CEF) was co-transfected with plasmid pSY681-HA7-LacZ and s-FPV-017 virus for homologous recombinant. Recombinant virus rFPV- H7HA was screened in the presence of X-gal to purify positive recombinant virus. The genome DNA of the recombinant fowl pox virus was used as template for PCR and 1.7 Kb HA gene fragments was obtained.This result indicated that the target gene was inserted into the genome DNA successfully. The results of Western-blot demonstrated that hemagglutinin glycoprotein of AIV was expressed efficiently in CEF infected with the recombinant pox virus in vitro.
Four- week–old SPF chickens were immunized with different doses of 106、104、102 PFU rFPV- H7HA and challenged with 100LD50 of HPAIV A/FPV/Rostock/34 (H7N1) through nostril .The throat and cloacal chamber swabs were collected 3、5、7 days after challenging. And chickens were observed daily for disease signs and deaths for 2 weeks. Sera were collected every week after vaccination for detecting the HI and AGP antibodies. Results shown that all chickens vaccinated with different doses of rFPV-H7HB generated high HI antibodies and were completely protected from virus challenge (no disease signs, no virus shedding and no deaths), but the control group died within eight days and can be detected high titers of H7 HPAIV from throat and cloacal chamber swabs. The results implied that the recombinant virus rFPV-H7HA that expressing HA gene of A/Chicken/Hebei/1/02(H7N2) would be the storage of vaccine and technology for preventing H7 subtypes HPAIV.
引文
1. Air, G. M., and Laver, W. G.. The neuraminidase of influenza virus. Proteins.1989,6:341~356.
2. Alexander, D.J,. A review of avian in?uenza. In Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on in?uenza viru ses of wild and domestic animals, Ghent, 16 /18 May 1999. Veterinary Microbiology, 74, 3 ~13.
3. Alexander, D.J.. Ecology of avian in?uenza in domestic birds. In B. Dodet & M. Vicari (Eds.), Proceedings of the International Symposium on Emergence and Control of Zoonotic Ortho~and Paramyxovirus Diseases. Merieux Foundation, Veyrier-du-lac, France. (2001) ,25 /34.
4. Almond, J. W. A single gene determines the host range of influenza viruses. Nature 1977; 270: 617~618.
5. Babiuk, L. A. Broadening the approaches to developing more effective vaccines.. Vaccine,1999 17:1587~1595.
6. Babiuk, L. A.. Broadening the approaches to developing more e.ective vaccines. Vaccine. 1999,17;1587~1595.
7. Baigent, S. J., R. C. Bethell, and J. W. McCauley. Genetic analysis reveals that both haemagglutinin and neuraminidase determine the sensitivity of naturally occurring avian influenza viruses to zanamivir in vitro. Virology 1999; 263: 323~338.
8. Baigent, S.J., and J. W. McCauley. Glycosylation of haemagglutinin and stalk~length of neuraminidase combine to regulate the growth of avian influenza viruses in tissue culture. Virus Res 2001; 79: 177~185.
9. Banbura, M. W., Y. Kawaoka, T. L. Thomas, and R. G. Webster. Reassortants with equine 1 (H7N7) influenza virus haemagglutinin in an avian influenza virus genetic background are pathogenic in chickens. Virology 1991; 184: 469~471.
10. Banks, J., E. C. Speidel, J. W. McCauley, and D. J. Alexander. Phylogenetic analysis of H7 haemagglutinin subtype influenza A viruses. Arch Virol 2000; 145: 1047~1058.
11. Banks, J., E.C. Speidel, E. Moore, L. Plowright, A. Piccirillo, I. Capua, P. Cordioli, A.Fioretti, and D. J. Alexander. Changes in the haemagglutinin and the neuraminidase genes prior to the emergence of highly pathogenic H7N1 avian influenza viruses in Italy. Arch Virol 2001; 146: 963~973.
12. Bano, S., Naeem, K. & Malik, S.A. (2003). Evaluation of pathogenic potential of avian in?uenza virus serotype H9N2 in chickens. Proceeding of the Fifth International Symposium on Avian In?uenza, Athens, Georgia, April 14 /17 2002. Avian Diseases , 47 , 817~822.
13. Baron, M. D., and Barrett, T. 1997. Rescue of rinderpest virus from cloned cDNA. J. Virol. 71, 1265~1271.
14. Baum,L. G., and J. C. Paulson. The N2 neuraminidase of human influenza viruses has acquired a substrate specificity complimentary to the haemagglutinin receptor specificity. Virology 1991; 180: 10~15.
15. Bazzigher, L., A. Schwarz, and P. Staeheli. No enhanced influenza virus resistance of murine and avian cells expressing cloned duck Mx protein. Virology 1993; 195: 100~112.
16. Beard, C. W., M. Brugh, and R. G. Webster. Emergence of amantadine~resisitant H5N2 avian influenza virus during a simulated layer flock treatment program. 1987. Avian Dis. 31:533~537.
17. Beard, C. W., Schnitzlein, W. M., Tripathy, D. N. Protection of chickens against highly pathogenic avian influenza virus (H5N2) by recombinant fowlpox viruses. Avian Dis. 1991. 35:356~359.
18. Beaton, A. R., and R. M. Krug. 1986. Transcription antitermination during influenza viral template RNA synthesis requires the nucleocapsid protein and the absence of a 5’ capped end. Proc. Natl. Acad. Sci. USA 83:8282~8286.
19. Bennink, J. R., J. W. Yewdell, G. L. Smith, and B. Moss. Anti~influenza virus cytotoxic T lymphocytes recognize the three viral polymerases and a nonstructural protein: responsiveness to individual viral antigens is major histocompatibility complex controlled. J Virol 1987; 61: 1098~1102.
20. Blok, J., and G. M. Air. Variation in the membrane~insertion and stalk sequences in eight sub~types of influenza type A virus neuraminidase. Biochem 1982; 21: 4001~4007.
21. Breuning, A., and C. Scholtissek. A reassortant between influenza A viruses (H7N2) synthesizing an enzymatically inactive neuraminidase at 40°C which is not incorporated into infectious particles. Virology 1986; 159: 65~74.
22. Bridgen, A., and Elliott, R. M. 1996. Rescue of a segmented negative strand RNA virus entirely form cloned complementary DNAs. Proc. Natl. Acad. Sci. USA 93. 15400~15404.
23. Buckler~White, A. J, C. W. Naeve, B. R. Murphy. Characterization of a gene coding for M proteins which is involved in host range restriction of an avian influenza A virus in monkeys. J Virol 1986; 57: 697~700.
24. Campbell, G., (1998). Report of the Irish national reference laboratory for 1996 and 1997. In Proceedings of the Joint Fourth Annual Meetings of the National Newcastle Disease and Avian In?uenza Laboratories of Countries of the European Union (p. 13). Brussels, Belgium.
25. Capua, I., and D. J. Alexander. Avian influenza: recent developments. Avian Pathol. 2004. 33:393~404.
26. Capua, I., C. Terregino, G. Cattoli, F. Mutinelli, and J. F. Rodriguez. Avian Pathol. 2002. 32:47~55.
27. Cassen, U., Collins, F. M., Dupres, W. P., and Rima, B. K. 2000. Establishment of a rescue system for canine distemper virus. J. Virol. 74. 10737~10744.
28. Castrucci, M. R., and Y. Kawaoka. Biologic importance of neuraminidase stalk length in influenza A virus. J Virol 1993; 67: 759~764.
29. Castrucci, M. R., S. Hou, P. C. Doherty, and Y. Kawaoka. Protection against lethal lymphocytic choriomeningitis virus (LCMV) infection by immunization of mice with an influenza virus containing an LCMV epitope recognized by cytotoxic T lymphocytes. 1994. J. Virol. 68:3486~3490.
30. Chen H, Subbarao K, Swayne D, Chen Q, Lu X, Katz J, Cox N, Matsuoka Y. Generation and evaluation of a high~growth reassortant H9N2 influenza A virus as a pandemic vaccine candidate. Vaccine 2003. 21:1974~1979.
31. Chen, H., Y. Matsuoka, D. Swayne, Q. Chen, N. J. Cox, B. R. Murphy, K. Subbarao.Generation and characterization of a cold~adapted influenza A H9N2 reassortant as a live pandemic influenza virus vaccine candidate. 2003. Vaccine 21:4430~4436.
32. Chen, W., P. A. Calvo, D. Malide1, J. Gibbs, U. Schubert, I. Bacik, S. Basta, R. O. Neill, J. Schickli, P. Palese, P. Henklein, J. R. Bennink, and J. W. Yewdell. A novel influenza A virus mitochondrial protein that induces cell death. Nature Medicine 2001; 7: 1306~1312.
33. Cheung, C. Y., L. L. M. Poon, A. S. Lau, W. Luk, Y. L. Lau, K. F. Shortridge, S. Gordon, Y. Guan, J. S. M. Peiris. Induction of pro~inflammatory cytokines in human macrophages by influenza A (H5N1) viruses: a mechanism for the unusual severity of human disease? Lancet 2002; 360: 1831~1837.
34. Chin, P. S., E. Hoffmann, R. Webby, R. G. Webster, Y. Guan, M. Peiris, and K. F. Shortridge. 2002. Molecular evolution of H6 influenza viruses from poultry in Southeastern China: prevalence of H6N1 influenza viruses possessing seven A/Hong Kong/156/97 (H5N1)~like genes in poultry. J. Virol. 76:507~516.
35. Choi, Y. K., Ozaki, H., Webby, R. J., Webster, R. G., Peiris, J. S., Poon, L., Butt, C., Leung, Y. H. C., and Guan. Y. 2004. Continuing evolution of H9N2 influenza viruses in southeastern China. J. Virol. 78, 8609~8614.
36. Claas, E., A. Osterhaus, R. van Beek, J. De Jong, , G. Rimmelzwaan, D. Senne, S. Krauss, K. Shortridge, and R. G. Webster. Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus (1998) Lancet 351, 472–477.
37. Clarke, D. K., Sidhu, M. S., Johnson, J. E., and Udem, S. 2000. Rescue of mumps virus from CDNA. J. Virol. 74, 4831~4838.
38. Connor, R. J., Y. Kawaoka, R. G. Webster, J. C. Paulson. Receptor specificity in human, avian and equine H2 and H3 influenza virus isolates. Virology 1994; 205: 17~23.
39. Crawford, J. M., Garcia, M., Stone, H., Swayne, D., Slemons, R., Perdue, M. L. molecular characterization of the hemagglutinin gene and oral immunization with a waterfowl~origin avian influenza virus. Avian Dis. 1998. 42:486~496.
40. Crawford, J., Wilkinson, B., Vosnesensky, A, Smith, G., Garcia, M., Stone, H., Perdue, M. L. Baculovirus~derived hemagglutinin vaccines protect against lethal influenza infectionsby avian H5 and H7 subtypes. Vaccine 1999. 17:2265~2274.
41. Deshpande, K. L., V. A. Fried, M. Ando, and R. G. Webster. Glycosylation affects cleavage of an H5N2 influenza virus haemagglutinin and regulates virulence. PNAS 1987; 84: 36~40.
42. Dybing,J. K., S. Schultz~Cherry, D. E. Swayne, D. L. Suarez, and M. L. Perdue. Distinct pathogenesis of Hong Kong~origin H5N1 viruses in mice compared to that of other highly pathogenic H5 avian influenza viruses. J Virol 2000; 74: 1443~1450.
43. Earn D. J., J. Dushoff, and S. A. Levin. Ecology and evoluation of the flu. Trends in Ecology& Evolution, 2002, 17(7):334~~40.
44. Eichelberger, M., W. Allan, M. Zijlstra, R. Jaenisch, and P. C. Doherty. Clearance of influenza virus respiratory infection in mice lacking class I major histocompatibility complex~restricted CD8+ T cells. J Exp Med 1991; 174: 875~880.
45. Els, M. C., G. M. Air, K. G. Murti, R. G. Webster, and W. G. Laver. An 18~amino acid deletion in an influenza neuraminidase. Virology 1985; 142: 241~247.
46. Enami, m., Sharma, G., Benham, C., and Palese, P. 1991. An influenza virus containing nine different RNA segments. Virology 185, 291~298.
47. Enami, M., W. Luytjes, M. Krystal, and P. Palese. 1990. Introduction of site~specific mutations into the genome of influenza virus. Proc. Natl. Acad. Sci. USA 87:3802~3805.
48. Feldmann, A., Schafer, M.K., Garten, W. & Klenk, H.D. (2000). Targeted infection of endothelial cells by avian influenza virus A/FPV/Rostock/34 (H7N1) in chicken embryos. Journal of Virology, 74 , 8018 ~/8027.
49. Fioretti, A., Menna, L.F. & Calabria, M., (1998). The epidemiological situation of avian in?uenza in Italy during 1996~1997. In Proceedings of the Joint Fourth Annual Meetings of the National Newcastle Disease and Avian In?uenza Laboratories of Countries of the European Union (pp. 17~ /22). Brussels, Belgium.
50. Fleming, D. M. 2001. Managing influenza: amantadine, rimantadine and beyond. Int. J. Clin. Pract. 55:189~195.
51. Fodor, E., Devenish, L., Engelhardt, O. G., Palese, P., Brownlee, G. G., and Garcia~Sastre, A. 1999. Rescue of influenza A virus from recombinant DNA. J. Virol. 73, 9679~9682.
52. Fouchier, R. A., P. M. Schneeberger, F. W. Rozendaal, J. M. Broekman, S. A. Kemink, V. Munster, T. Kuiken, G. F. Rimmelzwaan, M. Schutten, G. J. Van Doornum, G. Koch, A. Bosman, M. Koopmans, and A. D. Osterhaus. 2004. Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome. Proc. Natl. Acad. Sci. USA 101:1356~1361.
53. Gambaryan, A. S., V. E. Piskarev, I. A. Yamskov, A. M. Sakharov, A. B. Tuzikov , N. V. Bovin, N. E. Nifantev, and M. N. Matrosovich. Human influenza virus recognition of oligosaccharides. FEBS Letters 1995; 366: 57~60.
54. Garcia A, Johnson H, Srivastava DK, Jayawardene DA, Wehr DR, Webster RG. Efficacy of inactivated H5N2 influenza vaccines against lethal A/Chicken/Queretaro/19/95 infection. 1998. Avian Dis. 42(2):248~56.
55. Garcia, M., Crawford, J.M., Latimer, J.W., RiveraCruz, E. & Perdue, M.L. (1996). Heterogeneity in the haemagglutinin gene and emergence of the highly pathogenic phenotype among recent H5N2 avian in?uenza viruses from Mexico. J. Gen. Virol. 77: 1493~1504.
56. Garcia~Sastre, A., Muster, T., Barclay, W. D., Percy, N., and Palese, P. 1994. Use of a mammalian internal ribosomal entry site element for expression of a foreign protein by a transfectant influenza virus. J. Virol. 68:6254~6261.
57. Gibson, C. A., R. S. Daniels, J. S. Oxford, J. W. McCauley. Sequence analysis of the equine H7 influenza virus haemagglutinin gene. Virus Res 1992; 22: 93~106.
58. Gomez~Puertas P., Mena, I., Castillo M., Vivo, A., Perez~Pastrana, E., and Portela, A. 1999. Efficient formation of influenza virus~like particles: Dependence on the expression levels of viral proteins. J. Gen. Virol. 80, 1635~1645.
59. Goto, H., K. Wells, A. Takada, and Y. Kawaoka. Plasminogen~binding activity of neuraminidase determines the pathogenicity of influenza A virus. J Virol 2001; 75: 9297~9301.
60. Guan, Y., K. F. Shortridge, S. Krauss, and R. G. Webster. 1999. Molecular characterization of H9N2 influenza viruses; were they the donors of the “internal” genes of H5N1 viruses in Hong Kong? Proc. Natl. Acad. Sci. USA 96:9363~9367.
61. Horimoto, Y., Y. Yamazaki, T. Fukushima, T. Saito, S. E. Lindstrom, K. Omoe, R. Nerome, W. Lim, S. Sugita, and K. Nerome. 2000. Evolutionary characterization of the six internal genes of H5N1 human influenza A virus. J. Gen. Virol. 81:1293~1303.
62. Hughson, F. M. 1995. Structural characterization of viral fusion proteins. Curr. Biol. 5:265~274.
63. Hunt, L. A., Brown, D. W., Robinson, H. L., Naeve, C. W., Webster, R. G. Retrovirus~expressed hemagglutinin protects against lethal influenza virus infections. J. Virol. 1988. 62:3014~3019.
64. Inkster, M. D., V. S. Hinshaw, and I. T. Schulze. The haemagglutinins of duck and human H1 influenza viruses differ in sequence conservation and in glycosylation. J Virol 1993; 67: 7436~7443.
65. Ito, T., J. N. Couceiro, S. Kelm, L. G. Baum, S. Krauss, M. R. Castrucci, I. Donatelli, H. Kida, J. C. Paulson, R. G. Webster, and Y. Kawaoka. 1998. Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. J. Virol. 72:7367~7373.
66. Iwahashi, J., K. Tsuji, T. Ishibashi, J. Kajiwara, Y. Imamura, R. Mori, K. Hara, T. Kashiwagi, Y. Ohtsu, N. Hamada, H. Maeda, M. Toyoda, and T. Toyoda. 2001. Isolation of amantadine~resistant influenza A viruses (H3N2) from patients following administration of amantadine in Japan. J. Clin. Microbiol. 39:1652~1653.
67. Kaverin, V. V., Matrosovich, M. N., Gambaryan, A. S., Rudneva, I. A., Shilov, A. A., Varich, N. L., Makarova, N. V., Kropotkina, E. A., and Sinitsin, B. V. 2000. Intergenic HA~NA interactions in influenza A virus: postreassortant substitutions of charged amino acid in the hemagglutinin of different subtypes. Virus Res. 66:123~129.
68. Kawaoka, Y., O. T. Gorman, T. Ito, K. Wells, R. O. Donis, M. R. Castrucci, I. Donatelli, R. G. Webster. Influence of host species on the evolution of the nonstructural (NS) gene of influenza A viruses. Virus Res 1998: 55: 143~156.
69. Kawaoka, Y., S. Krauss, R. G. Webster. Avian~to~human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics. J Virol 1989; 63: 4603~4608.
70. Kida, H, T. Ito, J. Yashuda, Y. Shimizu, C. Itakura, K. F. Shortridge, Y. Kawaoka, and R.G. Webster. Potential for transmission of avian influenza viruses to pigs. J Gen Virol 1994; 75: 2183~2188.
71. Kilbourne, E. D. 1969. Future influenza vaccines and the use of genetic recombinants. Bull. World Health Organ. 41:643~645.
72. Kishida, N., Y. Sakoda, M. Eto, Y. Sunaga, and H. Kida. 2004. Co~infection of staphylococcus aureus or haemophilus paragallinarum exacerbates H9N2 influenza A virus infection in chickens. Arch Virol. 149:2095~2104.
73. Kiso, M., Mitamura, K., Tagawa, Y. S., Shiraishi, K., Kawakami, C., Kimura, K., Hayden, F. G., Sugaya, N., and Kawaoka, Y. (2004). Resistant influenza A viruses in children treated with oseltamivir: descriptive study. Lancet. 364, 759~65.
74. Klumpp, K., R. W. H. Ruigrok, F. Baudin. Roles of the influenza virus polymerase and nucleoprotein in forming a functional RNP structure. EMBO J 16:1248~1257.
75. Ko, J.~H., H.~K. Jin, A. Asano, A. Takada, A. Ninomiya, H. Kida, H. Hokiyama, M. Ohara, M. Tsuzuki, M. Nishibori, M. Mitzutani, and T. Watanabe. 2002. Polymorphisms and the differential antiviral activity of the chicken Mx gene. Genome Res. 12:595~601.
76. Kobasa, D., K. Wells, and Y. Kawaoka. Amino acids responsible for the absolute sialidase activity of the influenza A virus neuraminidase: relationship to growth in the duck intestine. J Virol 2001; 75: 11773~11780.
77. Kobasa, D., S. Kodihalli, M. Luo, M. R. Castrucci, I. Donatelli, T. Suzuki, and Y. Kawaoka. Amino acid residues contributing to the substrate specificity of the influenza A neuraminidase. J Virol. 1999; 73: 6743~6751.
78. Koolen, M. J. M., M. A. J. Borst, M. C. Horzinek, and W. J. M. Spaan. 1990. Immunogenic peptide comprising a mouse hepatitis virus A59 B~cell epitope and an influenza virus T~cell epitope protects against lethal infection. J. Virol. 64:6270~6273.
79. Koopmans, M., B. Wilbrink, M. Conyn, G. Natrop, H. van der Nat, H. Vennema, A. Meijer, J. van Steenbergen, R. Fouchier, A. Osterhaus, A. Bosman. Transmission of H7N7 avian influenza A virus to human beings during a large outbreak in commercial poultry farms in the Netherlands. 2004. 363:587~593.
80. Krug, R. M. Priming of influenza viral RNA transcription by capped heterologous RNAs.Curr. Top. Microbiol. Immunol. 93:125~149.
81. Lee, C.W., D. A. Senne, and D. L. Suarez. (2004). Effect of vaccine use in the evolution of Mexican lineage H5N2 avian influenza virus. J. Virol. 78, 8372~8381.
82. Lee, C.~W., Song, C.~S., Lee, Y.~J., Mo, I.~P., Garcia, M., Suarez, D.L. & Kim, S.~J. (2000). Sequence analysis of the haemagglutinin gene of H9N2 Korean avian in?uenza viruses and assessment of the pathogenic potential of isolate MS96. Avian Diseases , 44 , 527~535.
83. Li, K. S., Xu, K. M., Peiris, J. S. M., Poon, L. L. M., Yu, K. Z., Yuen, K. Y., Shortridge, K. F., Webster, R. G., and Guan, Y. 2003. Characterization of H9 subtype influenza viruses from the ducks of southern China: a candidate for the next influenza pandemic in humans? J. Virol. 77, 6988~6994.
84. Li, S. Q., C. G. Liu, A. Klimov, K. Subbarao, M. L. Perdue, D. Mo, Y. L. Ji, L. Woods, S. Hietala, and M. Bryant. 1999. Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses.
85. Li, S., Polonis, V., Isobe, H., Zaghouani, H., Guinea, R., Morran, T., Bona, C., and Palese, P. 1993. Chimeric influenza virus induces neutralizing antibodies and cytotoxic T cells against human immunodeficiency virus type 1. J. Virol. 67: 6659~6666.
86. Lin, Y. P., Shaw, M., Gregory, V., Cameron, K., Lim, W., Klimov, A., Subbarao, K., Guan, Y. L., Krauss, S., Shortridge, K., Webster, R., Cox, N., and Hay, A. (2000). Avian~to~human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates. Proc. Natl. Acad. Sci USA 97, 9654~9658.
87. Liu, J. H., Okazaki, K., Shi, W. M., Wu, Q. M., Mweene, A. S., and Kida, H. (2003b). Phylogenetic analysis of neuraminidase gene of H9N2 influenza viruses prevalent in chickens in China during 1995~2002. Virus Genes. 27, 197~202.
88. Liu, J., Okazaki, K., Ozaki, H., Sakoda, Y., Wu, Q., Chen, F. & Kido, H. (2003). H9N2 in?uenza viruses prevalent in China are phylogenetically distinct from A/quail/Hong Kong/G1/97 presumed to be the donor of the internal protein genes of the H5N1 Hong Kong/97 virus. Avian Pathology, 32 , 551 /560.
89. Liu, M., He, S. Q., Walker, D., Zhou, N. N., Perez, D. R., Mo, B., Li, F., Huang, X. T.,Webster, R. G., and Webby, R. J. (2003). The influenza gene pool in a poultry market in south central China. Virology 305, 267~275.
90. Lu, J. H., X. F. Liu, W. X. Shao, P. H. Zhang, and D. P. Wei. 2003. Genetic Characterization of the Entire Genome of an H9N2 Avian Influenza Virus A/Chicken/Shanghai/F/98. Acta Microbiologica Sinica 43:434~441.
91. Lu, X. H., Renshaw, M., Tumpey, T. M., Kelly, G. D., Primmer, J. H., and Katz, J. M. (2001). Immunity to influenza A H9N2 viruses induced by infection and vaccination. J. Virol. 75, 4896~4901.
92. Lu, Y., M. Wambach, M. G. Katze, and R. M. Krug. Binding of the influenza virus NS1 protein to double~stranded RNA inhibits the activation of the protein kinase that phosphorylated the eIF~2 translation initiation factor. Virology 1995; 214: 222~228.
93. Ludwig, S., X. Wang, C. Ehrhardt, H. Zheng, N. Donelan, O. Planz, S. Pleschka, A. Garcia~Sastre, G. Heins, and T. Wolff. The influenza A virus NS1 protein inhibits activation of Jun N~terminal kinase and AP~1 transcription factors. J Virol 2002; 76: 11166~11171.
94. Luo, G., J. Chung, and P. Palese. Alterations of the stalk of the influenza virus neuraminidase: deletions and insertions. Virus Res 1993; 29: 141~153.
95. Luytjes, W., D. Geerts, W. Posthumus, R. Meloen, and W. Spaan. 1989. Amino acid sequence of a conserved neutralizing epitope of murine coronaviruses. J. Virol. 63:1408~1412.
96. Luytjes, W., L. S. Sturman, P. J. Bredenbeek, J. Charite, B. A. van der Zeijst, M. C. Horzinek, and W. J. Spaan. 1987. Primary structure of the glycoprotein E2 of coronavirus MHV~A59 and identification of the trypsin cleavage site. Virology 161:479~487.
97. Luytjes, W., M. Krystal, M. Enami, J. D. Pavin, and P. Palese. 1989. Amplification, expression and packaging of a foreign gene by influenza virus. Cell. 58:1107~1113.
98. Leong KH, Rainsay AJ, Boyle DB, Ramshaw IA. 1994. Selective induction of immune response by cytokines coexpressed in recombinant fowlpox virus. J Virol, 68: 8125~30.
99. Maassab, H. F., and Bryant, M. L. 1999. The development of live attenuated cold~adapted influenza virus vaccine for humans. Rev. Med. Virol. 9, 237~244.
100.Mase M, Imada T, Sanada Y, Etoh M, Sanada N, Tsukamoto K, Kawaoka Y, Yamaguchi S: Imported parakeets harbor H9N2 influenza A viruses that are genetically closely related to those transmitted to humans in Hong Kong. J Virol 75:3490~3494, 2001.
101.Matrosovich, M. N., S. Krauss, R.G. Webster. H9N2 influenza A viruses from poultry in Asia have human virus~like receptor specificity. Virology 2001; 281: 156~162.
102.Matrosovich, M., N. Tuzikov, A. Bovin, A. Gambaryan, A. Klimov, M.R. Castrucci, I. Donatelli, and Y. Kawaoka. Early alterations of the receptor~binding properties of H1, H2, H3 avian influenza virus haemagglutinins after their introduction into mammals. J Virol 2000; 74: 8502~8512.
103.Matrosovich,M., N. Zhou, Y. Kawaoka, and R. Webster. The surface glycoproteins of H5 influenza viruses from humans, chickens, and wild aquatic birds have distinguishable properties. J Virol 1999; 73: 1146~1155.
104. McKimm~Breschkin, J. L. 2000. Resistance of influenza viruses to neuraminidase inhibitors—a review. Antivir. Res. 47:1~17.
105.Mebatsion, T., M. J. Koolen, L. T. de Vaan, N. de Haas, M. Braber, A. Romer~Oberdorfer, P. van den Elzen, and P. van der Marel. 2002. Newcastle disease virus (NDV) marker vaccine: an immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope. J. Virol. 76:10138–10146.
106. Mebatsion, T., M. J. Koolen, L. T. de Vaan, N. de Haas, M. Braber, A. Romer~Oberdorfer, P. van den Elzen, and P. van der Marel. 2002. Newcastle disease virus (NDV) marker vaccine: an immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope. J. Virol. 76:10138~10146.
107. Mendel, D. B., C. Y. Tai, P. A. Escarpe, W. X. Li, R. W. Sidwell, J. H. Huffman, C. Sweet, K. J. Jakeman, J. Merson, S. A. Lacy, W. Lew, M. A. Williams, L. Zhang, M. S. Chen, N. Bishofberger, and C. U. Kim. 1998. Oral administration of a prodrug of the influenza virus neuraminidase inhibitor GS 4071 protects mice and ferrets against influenza infection. Antimicrob. Agents Chemother. 42:640~646
108. Mitnaul, L. J., Matrosovich, M. N., Castrucci, M. R., Tuzikov, A. B., Bovin, N. V., Kobasa, D.,and Kawaoka, Y. 2000. Balanced hemagglutinin and neuraminidase activitiesare critical for efficient replication of influenza A virus. J. Virol. 74:6015~6020.
109.Mo, I.P., Song, C.S., Kim, K.S. & Rhee, J.C. (1998). An occurrence of non~highly pathogenic avian in?uenza in Korea. In Proceedings of the 4th International Symposium on Avian In?uenza , 1997 (pp. 379~383). Athens, Georgia, USA.
110.Morris, S. J, G. E. Price, J. M. Barnett, S. A. Hiscox, H. Smith, and C. Sweet. Role of neuraminidase in influenza virus~induced apoptosis. J Gen Virol 1999; 80: 137~146.
111.Muster, T., Ferko, B., Klima, A., Purtscher, M., Trkola, A., Schulz, P., Grassauer, A., Engelhardt, O.G., Garcia~Sastre, A., Palese, P., and Katinger, H. 1995. Mucosal model of immunization against human immunodeficiency virus type 1 with a chimeric influenza virus. J. Virol. 69, 6678~6686.
112.Muster, T., Guinea, R., Trkola, A., Purtscher, M., Klima, A., Steindl, F., Palese, P., and Katinger, H. 1994. Cross~neutralizing activity against divergent human immunodeficiency virus type 1 isolates induced by the gp41sequence ELDKWAS. J. Virol. 68: 4031~4034.
113.Naeem, K., Naurin, M., Rashid, S. & Bano, S. (2003). Seroprevalence of avian in?uenza virus and its relationship with increased mortality and decreased egg production. Avian Pathology, 32 , 283~287.
114.Naeem, K., Ullah, A., Manvell, R.J.& Alexander, D.J. (1999). Avian in?uenza A subtype H9N2 in poultry in Pakistan. Veterinary Record , 145 , 560.
115. Naffakh, N., P. Massin, N. Escriou, B. Crescenzo~Chaigne, and S. van der Werf. 2000. Genetic analysis of the compatibility between olymerase proteins from humans and avian strains of influenza A viruses. J. Gen. Virol. 81:1283~1291.
116.Neumann, G., A. Zobel, and G. Hobom. 1994. RNA polymerase I~mediated expression of influenza viral RNA molecules. Virology 202:477~479.
117.Neumann, G., Hughes, M. T., and Kawaoka, Y. Influenza A virus NS2 protein mediates Vrnp nuclear export through NES~independent interactionwith hCRM1. EMBO J. 2000,19:6751~6758.
118.Neumann, G., Watanabe, T., and Kawaoka, Y. Plasmid~driven formation of influenza virus~like particles. J. Virol. 2000,547~551.
119.Neumann, G., Watanabe, T., Ito, H., Watanabe, S., Goto, H., Gao, P., Hughes, M., Perez, D., Donis, R., Hoffmann, E., Hobom, G., and Kawaka, . 1999. Generation of influenza A viruses entirely from cloned cDNAs. Proc. Natl. Acad. Sci. 96, 9345~9350.
120.Nili, H. & Asasi, K. (2002). Natural cases and an experimental study of H9N2 avian in?uenza in commercial broiler chickens of Iran. Avian Pathology, 31:247~252.
121.Nili, H. & Asasi, K. (2003). Avian In?uenza (H9N2) outbreak in Iran. In: Proceedings of the Fifth International Symposium on Avian In?uenza, Athens, Georgia, April 14~17 2002. Avian Diseases , 47 , 828~831.
122.Nobusawa, E., T. Aoyama, H. Kato, Y. Suzuki, Y. Tateno, and K. Nakajima. Comparison of complete amino acid sequences and receptor~binding properties among 13 serotypes of haemagglutinins of influenza A viruses. Virology .1991,182: 475~485.
123.Ohuchi, M., M. Orlich, R. Ohuchi, B. E. Simpson, W. Garten, H. D. Klenk, and R. Rott. Mutations at the cleavage site of the haemagglutinin alter the pathogenicity of influenza virus A/Chick/Penn/83 [H5N2]. Virology .1989; 168: 274~280.
124. Ohuchi, M., Orlich, M., Ohuchi, R., Simpson, B. E., Garten, W., Klenk, H. D., and Rott, R. Mutations at the cleavage site of the hemagglutinin alter the pathogenicity of influenza virus A/Chick/Penn/83(H5N2). Virology 168:274~280.
125.Ohuchi, M., R. Ohuchi, A. Feldmann, and H. K. Klenk. Regulation of receptor binding affinity of influenza virus haemagglutinin by its carbohydrate moiety. J Virol 1997,71: 8377~8384.
126. Ohuchi, R., Ohuchi, M., Garten, W., and Klenk, H. D. Oligo~saccharides in the stem region maintain the influenza virus hemagglutinin in the metastable forn required for fusion activity. 1997. J. Virol. 71:3719~3725.
127.Okazaki, K., Y. Kawaoka, R. G. Webster. Evolutionary pathways of the PA genes of influenza A viruses. Virology 1989. 172: 601~608.
128.O'Neill, E., S. L. Krauss, J. M. Riberdy, R. G. Webster, and D. L. Woodland. Heterologous protection against lethal A/HongKong/156/97 (H5N1) influenza virus infection in C57BL/6 mice. J Gen Virol 2000; 81: 2689~2696.
129.Ozaki, H., Govorkova, E. A., Li,C. Xiong, X., Webster, R. G., and Webby, R. J.Generation of high~yielding influenza A viruses in African green monkey kidney cells by reverse genetics. J. Virol. 2004, 78, 1851~1857.
130.Parvin, J. D., P. Palese, A. Honda, A. Ishihama, and M. Krystal. Promoter analysis of influenza virus RNA polymerase. J. Virol. 1989,68:5142~5152.
131.陈化兰,于康震,田国斌等.DNA免疫诱导鸡对禽流感病毒的免疫保护反应.中国农业科学,1998,31:63~68
132.陈化兰,于康震,田国斌等. H5和H7亚型禽流感病毒HA基因的RT~PCR扩增及克隆.中国畜禽传染病,1997,2: 160~180.
133.陈化兰,于康震,田国斌等.禽流感病毒血凝素基因的克隆及其DNA疫苗的免疫源性研究 ,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编,中国农业科技出版社).1998,5: 356~3590.
134.李海燕,辛晓光,田国斌等.禽流感抗体间接酶联免疫吸附试验检测法的建立.中国畜禽传染病,1998,20增刊:157~159.
135.陈伯伦,张泽纪,陈伟斌.关于禽流感几个问题的看法.中国畜禽传染病,1998,20增刊 : 230~231.
136.陈伯伦,张泽纪,陈伟斌.当前防制禽流感的建议.中国畜禽传染病,1998,20增刊:2 31 ~233.
137.陈化兰,于康震,卢景良.禽流感病毒及其分子生物学研究进展.畜禽重大疫病生物技术防制研究(白新盛,卢景良主编).中国农业科技出版社,1998,5:313~320.
138.李海燕,于康震,张晶.禽流感诊断技术研究进展,畜禽重大疫病生物技术防制研究.中国农业科技出版社,1998,5:329~334.
139.陈化兰,于康震.基因免疫研究进展,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编.中国农业科技出版社,1998,5:325~328.
140.崔尚金,陈化兰,邓国华等.禽流感RT~PCR诊断法的建立,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编).中国农业科技出版社1998,5:329~334.
141.邓国华,于康震.H5亚型禽流感病毒核蛋白基因的扩增与克隆,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编).中国农业科技出版社,1998,5:348~351.
142.田国斌,唐秀英,于康震.禽流感病毒感染鸡抗体的监测及血凝素分型血清的制备,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编).中国农业科技出版社,1998,5:352~335.
143.田国斌,赵增连,唐秀英等.鸭源禽流感病毒的分离和鉴定.中国预防兽医学报, 1999,21(1):1 ~3.
144.邓国华,马文军,于康震.禽流感病毒核蛋白基因在杆状病毒中的表达.中国预防兽医学报,1999,21(2):81~ 83.
145.刘明.H5和H7亚型禽流感分子防制技术的研究[博士论文].中国农业科学院哈尔滨兽医研究所,2000,6.
146.张建林.禽流感病毒A/Goose/GuangDONG/1/96( H5Nl) 分离株全基因序列分析[硕士论文].中国农业科学院哈尔滨兽医研究所,2000,6.
147.李一经,刘胜旺,陈立君等.马立克氏病弱毒苗接种鸡T细胞亚群观察.中国畜禽 传染病,1997,20( 6):345~347.
148.李一经,刘胜旺,陈立君等.马立克氏病毒强毒株感染鸡T细胞表型动态变化的研究 .上海免疫学杂志,1998,18(5):308~309,311.
149.贾永清,陈化兰,邓国华等.禽流感病毒分离株A/Goose/Guangdong/3/96(H 5N 1) N A 基因序列分析.中国预防兽医学报,2000,22 (1):25~30.
150.乔传玲.表达禽流感病毒HA~NA、HA~NP及NP基因重组禽痘病毒的构建及其免疫效力的研究[博士论文].中国农业科学院哈尔滨兽医研究所,2002,6.
151.刘胜旺,李一经,曹殿军等.新城疫油苗免疫鸡血清1g抗体含量及T细胞亚类的观察.中国免疫学杂志,1999,14:65~67.
152.毕英佐,曹永长,朱基美.东禽流感的血清学调查.中国兽医杂志,1994, 20(7): 8–9.
153.程安春,汪铭书,廖德惠等.鸡A型流感病毒的分离及初步鉴定.四川畜牧兽医,1995,4:5~6.
154.陈伯伦,张泽纪,陈伟斌.禽流感研究鸡A型流感病毒的分离与血清学初步鉴定 . 中国兽医杂志,1994,2(10):3~7.
155.郭元吉,范瑞莲,曲风珍等.甲型流感病毒在我国动物中分布的初步调查.微生物学报,1981,21(3):379~384.
156.马文军.禽流感病毒血凝素基因重组禽痘病毒的构建[硕士研究论文].中国农业科学院哈尔滨兽医研究,1999.
157.甘孟侯.禽流感[M].第 2 版,北京:中国农业出版社.2002,98~100.
158.郭元吉,李建国,程小雯,等.禽 H9N2 亚型流感病毒能感染人的发现.中国实验和临床病毒学杂志,1999,13(2):105~108.
2. Alexander, D.J,. A review of avian in?uenza. In Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on in?uenza viru ses of wild and domestic animals, Ghent, 16 /18 May 1999. Veterinary Microbiology, 74, 3 ~13.
3. Alexander, D.J.. Ecology of avian in?uenza in domestic birds. In B. Dodet & M. Vicari (Eds.), Proceedings of the International Symposium on Emergence and Control of Zoonotic Ortho~and Paramyxovirus Diseases. Merieux Foundation, Veyrier-du-lac, France. (2001) ,25 /34.
4. Almond, J. W. A single gene determines the host range of influenza viruses. Nature 1977; 270: 617~618.
5. Babiuk, L. A. Broadening the approaches to developing more effective vaccines.. Vaccine,1999 17:1587~1595.
6. Babiuk, L. A.. Broadening the approaches to developing more e.ective vaccines. Vaccine. 1999,17;1587~1595.
7. Baigent, S. J., R. C. Bethell, and J. W. McCauley. Genetic analysis reveals that both haemagglutinin and neuraminidase determine the sensitivity of naturally occurring avian influenza viruses to zanamivir in vitro. Virology 1999; 263: 323~338.
8. Baigent, S.J., and J. W. McCauley. Glycosylation of haemagglutinin and stalk~length of neuraminidase combine to regulate the growth of avian influenza viruses in tissue culture. Virus Res 2001; 79: 177~185.
9. Banbura, M. W., Y. Kawaoka, T. L. Thomas, and R. G. Webster. Reassortants with equine 1 (H7N7) influenza virus haemagglutinin in an avian influenza virus genetic background are pathogenic in chickens. Virology 1991; 184: 469~471.
10. Banks, J., E. C. Speidel, J. W. McCauley, and D. J. Alexander. Phylogenetic analysis of H7 haemagglutinin subtype influenza A viruses. Arch Virol 2000; 145: 1047~1058.
11. Banks, J., E.C. Speidel, E. Moore, L. Plowright, A. Piccirillo, I. Capua, P. Cordioli, A.Fioretti, and D. J. Alexander. Changes in the haemagglutinin and the neuraminidase genes prior to the emergence of highly pathogenic H7N1 avian influenza viruses in Italy. Arch Virol 2001; 146: 963~973.
12. Bano, S., Naeem, K. & Malik, S.A. (2003). Evaluation of pathogenic potential of avian in?uenza virus serotype H9N2 in chickens. Proceeding of the Fifth International Symposium on Avian In?uenza, Athens, Georgia, April 14 /17 2002. Avian Diseases , 47 , 817~822.
13. Baron, M. D., and Barrett, T. 1997. Rescue of rinderpest virus from cloned cDNA. J. Virol. 71, 1265~1271.
14. Baum,L. G., and J. C. Paulson. The N2 neuraminidase of human influenza viruses has acquired a substrate specificity complimentary to the haemagglutinin receptor specificity. Virology 1991; 180: 10~15.
15. Bazzigher, L., A. Schwarz, and P. Staeheli. No enhanced influenza virus resistance of murine and avian cells expressing cloned duck Mx protein. Virology 1993; 195: 100~112.
16. Beard, C. W., M. Brugh, and R. G. Webster. Emergence of amantadine~resisitant H5N2 avian influenza virus during a simulated layer flock treatment program. 1987. Avian Dis. 31:533~537.
17. Beard, C. W., Schnitzlein, W. M., Tripathy, D. N. Protection of chickens against highly pathogenic avian influenza virus (H5N2) by recombinant fowlpox viruses. Avian Dis. 1991. 35:356~359.
18. Beaton, A. R., and R. M. Krug. 1986. Transcription antitermination during influenza viral template RNA synthesis requires the nucleocapsid protein and the absence of a 5’ capped end. Proc. Natl. Acad. Sci. USA 83:8282~8286.
19. Bennink, J. R., J. W. Yewdell, G. L. Smith, and B. Moss. Anti~influenza virus cytotoxic T lymphocytes recognize the three viral polymerases and a nonstructural protein: responsiveness to individual viral antigens is major histocompatibility complex controlled. J Virol 1987; 61: 1098~1102.
20. Blok, J., and G. M. Air. Variation in the membrane~insertion and stalk sequences in eight sub~types of influenza type A virus neuraminidase. Biochem 1982; 21: 4001~4007.
21. Breuning, A., and C. Scholtissek. A reassortant between influenza A viruses (H7N2) synthesizing an enzymatically inactive neuraminidase at 40°C which is not incorporated into infectious particles. Virology 1986; 159: 65~74.
22. Bridgen, A., and Elliott, R. M. 1996. Rescue of a segmented negative strand RNA virus entirely form cloned complementary DNAs. Proc. Natl. Acad. Sci. USA 93. 15400~15404.
23. Buckler~White, A. J, C. W. Naeve, B. R. Murphy. Characterization of a gene coding for M proteins which is involved in host range restriction of an avian influenza A virus in monkeys. J Virol 1986; 57: 697~700.
24. Campbell, G., (1998). Report of the Irish national reference laboratory for 1996 and 1997. In Proceedings of the Joint Fourth Annual Meetings of the National Newcastle Disease and Avian In?uenza Laboratories of Countries of the European Union (p. 13). Brussels, Belgium.
25. Capua, I., and D. J. Alexander. Avian influenza: recent developments. Avian Pathol. 2004. 33:393~404.
26. Capua, I., C. Terregino, G. Cattoli, F. Mutinelli, and J. F. Rodriguez. Avian Pathol. 2002. 32:47~55.
27. Cassen, U., Collins, F. M., Dupres, W. P., and Rima, B. K. 2000. Establishment of a rescue system for canine distemper virus. J. Virol. 74. 10737~10744.
28. Castrucci, M. R., and Y. Kawaoka. Biologic importance of neuraminidase stalk length in influenza A virus. J Virol 1993; 67: 759~764.
29. Castrucci, M. R., S. Hou, P. C. Doherty, and Y. Kawaoka. Protection against lethal lymphocytic choriomeningitis virus (LCMV) infection by immunization of mice with an influenza virus containing an LCMV epitope recognized by cytotoxic T lymphocytes. 1994. J. Virol. 68:3486~3490.
30. Chen H, Subbarao K, Swayne D, Chen Q, Lu X, Katz J, Cox N, Matsuoka Y. Generation and evaluation of a high~growth reassortant H9N2 influenza A virus as a pandemic vaccine candidate. Vaccine 2003. 21:1974~1979.
31. Chen, H., Y. Matsuoka, D. Swayne, Q. Chen, N. J. Cox, B. R. Murphy, K. Subbarao.Generation and characterization of a cold~adapted influenza A H9N2 reassortant as a live pandemic influenza virus vaccine candidate. 2003. Vaccine 21:4430~4436.
32. Chen, W., P. A. Calvo, D. Malide1, J. Gibbs, U. Schubert, I. Bacik, S. Basta, R. O. Neill, J. Schickli, P. Palese, P. Henklein, J. R. Bennink, and J. W. Yewdell. A novel influenza A virus mitochondrial protein that induces cell death. Nature Medicine 2001; 7: 1306~1312.
33. Cheung, C. Y., L. L. M. Poon, A. S. Lau, W. Luk, Y. L. Lau, K. F. Shortridge, S. Gordon, Y. Guan, J. S. M. Peiris. Induction of pro~inflammatory cytokines in human macrophages by influenza A (H5N1) viruses: a mechanism for the unusual severity of human disease? Lancet 2002; 360: 1831~1837.
34. Chin, P. S., E. Hoffmann, R. Webby, R. G. Webster, Y. Guan, M. Peiris, and K. F. Shortridge. 2002. Molecular evolution of H6 influenza viruses from poultry in Southeastern China: prevalence of H6N1 influenza viruses possessing seven A/Hong Kong/156/97 (H5N1)~like genes in poultry. J. Virol. 76:507~516.
35. Choi, Y. K., Ozaki, H., Webby, R. J., Webster, R. G., Peiris, J. S., Poon, L., Butt, C., Leung, Y. H. C., and Guan. Y. 2004. Continuing evolution of H9N2 influenza viruses in southeastern China. J. Virol. 78, 8609~8614.
36. Claas, E., A. Osterhaus, R. van Beek, J. De Jong, , G. Rimmelzwaan, D. Senne, S. Krauss, K. Shortridge, and R. G. Webster. Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus (1998) Lancet 351, 472–477.
37. Clarke, D. K., Sidhu, M. S., Johnson, J. E., and Udem, S. 2000. Rescue of mumps virus from CDNA. J. Virol. 74, 4831~4838.
38. Connor, R. J., Y. Kawaoka, R. G. Webster, J. C. Paulson. Receptor specificity in human, avian and equine H2 and H3 influenza virus isolates. Virology 1994; 205: 17~23.
39. Crawford, J. M., Garcia, M., Stone, H., Swayne, D., Slemons, R., Perdue, M. L. molecular characterization of the hemagglutinin gene and oral immunization with a waterfowl~origin avian influenza virus. Avian Dis. 1998. 42:486~496.
40. Crawford, J., Wilkinson, B., Vosnesensky, A, Smith, G., Garcia, M., Stone, H., Perdue, M. L. Baculovirus~derived hemagglutinin vaccines protect against lethal influenza infectionsby avian H5 and H7 subtypes. Vaccine 1999. 17:2265~2274.
41. Deshpande, K. L., V. A. Fried, M. Ando, and R. G. Webster. Glycosylation affects cleavage of an H5N2 influenza virus haemagglutinin and regulates virulence. PNAS 1987; 84: 36~40.
42. Dybing,J. K., S. Schultz~Cherry, D. E. Swayne, D. L. Suarez, and M. L. Perdue. Distinct pathogenesis of Hong Kong~origin H5N1 viruses in mice compared to that of other highly pathogenic H5 avian influenza viruses. J Virol 2000; 74: 1443~1450.
43. Earn D. J., J. Dushoff, and S. A. Levin. Ecology and evoluation of the flu. Trends in Ecology& Evolution, 2002, 17(7):334~~40.
44. Eichelberger, M., W. Allan, M. Zijlstra, R. Jaenisch, and P. C. Doherty. Clearance of influenza virus respiratory infection in mice lacking class I major histocompatibility complex~restricted CD8+ T cells. J Exp Med 1991; 174: 875~880.
45. Els, M. C., G. M. Air, K. G. Murti, R. G. Webster, and W. G. Laver. An 18~amino acid deletion in an influenza neuraminidase. Virology 1985; 142: 241~247.
46. Enami, m., Sharma, G., Benham, C., and Palese, P. 1991. An influenza virus containing nine different RNA segments. Virology 185, 291~298.
47. Enami, M., W. Luytjes, M. Krystal, and P. Palese. 1990. Introduction of site~specific mutations into the genome of influenza virus. Proc. Natl. Acad. Sci. USA 87:3802~3805.
48. Feldmann, A., Schafer, M.K., Garten, W. & Klenk, H.D. (2000). Targeted infection of endothelial cells by avian influenza virus A/FPV/Rostock/34 (H7N1) in chicken embryos. Journal of Virology, 74 , 8018 ~/8027.
49. Fioretti, A., Menna, L.F. & Calabria, M., (1998). The epidemiological situation of avian in?uenza in Italy during 1996~1997. In Proceedings of the Joint Fourth Annual Meetings of the National Newcastle Disease and Avian In?uenza Laboratories of Countries of the European Union (pp. 17~ /22). Brussels, Belgium.
50. Fleming, D. M. 2001. Managing influenza: amantadine, rimantadine and beyond. Int. J. Clin. Pract. 55:189~195.
51. Fodor, E., Devenish, L., Engelhardt, O. G., Palese, P., Brownlee, G. G., and Garcia~Sastre, A. 1999. Rescue of influenza A virus from recombinant DNA. J. Virol. 73, 9679~9682.
52. Fouchier, R. A., P. M. Schneeberger, F. W. Rozendaal, J. M. Broekman, S. A. Kemink, V. Munster, T. Kuiken, G. F. Rimmelzwaan, M. Schutten, G. J. Van Doornum, G. Koch, A. Bosman, M. Koopmans, and A. D. Osterhaus. 2004. Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome. Proc. Natl. Acad. Sci. USA 101:1356~1361.
53. Gambaryan, A. S., V. E. Piskarev, I. A. Yamskov, A. M. Sakharov, A. B. Tuzikov , N. V. Bovin, N. E. Nifantev, and M. N. Matrosovich. Human influenza virus recognition of oligosaccharides. FEBS Letters 1995; 366: 57~60.
54. Garcia A, Johnson H, Srivastava DK, Jayawardene DA, Wehr DR, Webster RG. Efficacy of inactivated H5N2 influenza vaccines against lethal A/Chicken/Queretaro/19/95 infection. 1998. Avian Dis. 42(2):248~56.
55. Garcia, M., Crawford, J.M., Latimer, J.W., RiveraCruz, E. & Perdue, M.L. (1996). Heterogeneity in the haemagglutinin gene and emergence of the highly pathogenic phenotype among recent H5N2 avian in?uenza viruses from Mexico. J. Gen. Virol. 77: 1493~1504.
56. Garcia~Sastre, A., Muster, T., Barclay, W. D., Percy, N., and Palese, P. 1994. Use of a mammalian internal ribosomal entry site element for expression of a foreign protein by a transfectant influenza virus. J. Virol. 68:6254~6261.
57. Gibson, C. A., R. S. Daniels, J. S. Oxford, J. W. McCauley. Sequence analysis of the equine H7 influenza virus haemagglutinin gene. Virus Res 1992; 22: 93~106.
58. Gomez~Puertas P., Mena, I., Castillo M., Vivo, A., Perez~Pastrana, E., and Portela, A. 1999. Efficient formation of influenza virus~like particles: Dependence on the expression levels of viral proteins. J. Gen. Virol. 80, 1635~1645.
59. Goto, H., K. Wells, A. Takada, and Y. Kawaoka. Plasminogen~binding activity of neuraminidase determines the pathogenicity of influenza A virus. J Virol 2001; 75: 9297~9301.
60. Guan, Y., K. F. Shortridge, S. Krauss, and R. G. Webster. 1999. Molecular characterization of H9N2 influenza viruses; were they the donors of the “internal” genes of H5N1 viruses in Hong Kong? Proc. Natl. Acad. Sci. USA 96:9363~9367.
61. Horimoto, Y., Y. Yamazaki, T. Fukushima, T. Saito, S. E. Lindstrom, K. Omoe, R. Nerome, W. Lim, S. Sugita, and K. Nerome. 2000. Evolutionary characterization of the six internal genes of H5N1 human influenza A virus. J. Gen. Virol. 81:1293~1303.
62. Hughson, F. M. 1995. Structural characterization of viral fusion proteins. Curr. Biol. 5:265~274.
63. Hunt, L. A., Brown, D. W., Robinson, H. L., Naeve, C. W., Webster, R. G. Retrovirus~expressed hemagglutinin protects against lethal influenza virus infections. J. Virol. 1988. 62:3014~3019.
64. Inkster, M. D., V. S. Hinshaw, and I. T. Schulze. The haemagglutinins of duck and human H1 influenza viruses differ in sequence conservation and in glycosylation. J Virol 1993; 67: 7436~7443.
65. Ito, T., J. N. Couceiro, S. Kelm, L. G. Baum, S. Krauss, M. R. Castrucci, I. Donatelli, H. Kida, J. C. Paulson, R. G. Webster, and Y. Kawaoka. 1998. Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. J. Virol. 72:7367~7373.
66. Iwahashi, J., K. Tsuji, T. Ishibashi, J. Kajiwara, Y. Imamura, R. Mori, K. Hara, T. Kashiwagi, Y. Ohtsu, N. Hamada, H. Maeda, M. Toyoda, and T. Toyoda. 2001. Isolation of amantadine~resistant influenza A viruses (H3N2) from patients following administration of amantadine in Japan. J. Clin. Microbiol. 39:1652~1653.
67. Kaverin, V. V., Matrosovich, M. N., Gambaryan, A. S., Rudneva, I. A., Shilov, A. A., Varich, N. L., Makarova, N. V., Kropotkina, E. A., and Sinitsin, B. V. 2000. Intergenic HA~NA interactions in influenza A virus: postreassortant substitutions of charged amino acid in the hemagglutinin of different subtypes. Virus Res. 66:123~129.
68. Kawaoka, Y., O. T. Gorman, T. Ito, K. Wells, R. O. Donis, M. R. Castrucci, I. Donatelli, R. G. Webster. Influence of host species on the evolution of the nonstructural (NS) gene of influenza A viruses. Virus Res 1998: 55: 143~156.
69. Kawaoka, Y., S. Krauss, R. G. Webster. Avian~to~human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics. J Virol 1989; 63: 4603~4608.
70. Kida, H, T. Ito, J. Yashuda, Y. Shimizu, C. Itakura, K. F. Shortridge, Y. Kawaoka, and R.G. Webster. Potential for transmission of avian influenza viruses to pigs. J Gen Virol 1994; 75: 2183~2188.
71. Kilbourne, E. D. 1969. Future influenza vaccines and the use of genetic recombinants. Bull. World Health Organ. 41:643~645.
72. Kishida, N., Y. Sakoda, M. Eto, Y. Sunaga, and H. Kida. 2004. Co~infection of staphylococcus aureus or haemophilus paragallinarum exacerbates H9N2 influenza A virus infection in chickens. Arch Virol. 149:2095~2104.
73. Kiso, M., Mitamura, K., Tagawa, Y. S., Shiraishi, K., Kawakami, C., Kimura, K., Hayden, F. G., Sugaya, N., and Kawaoka, Y. (2004). Resistant influenza A viruses in children treated with oseltamivir: descriptive study. Lancet. 364, 759~65.
74. Klumpp, K., R. W. H. Ruigrok, F. Baudin. Roles of the influenza virus polymerase and nucleoprotein in forming a functional RNP structure. EMBO J 16:1248~1257.
75. Ko, J.~H., H.~K. Jin, A. Asano, A. Takada, A. Ninomiya, H. Kida, H. Hokiyama, M. Ohara, M. Tsuzuki, M. Nishibori, M. Mitzutani, and T. Watanabe. 2002. Polymorphisms and the differential antiviral activity of the chicken Mx gene. Genome Res. 12:595~601.
76. Kobasa, D., K. Wells, and Y. Kawaoka. Amino acids responsible for the absolute sialidase activity of the influenza A virus neuraminidase: relationship to growth in the duck intestine. J Virol 2001; 75: 11773~11780.
77. Kobasa, D., S. Kodihalli, M. Luo, M. R. Castrucci, I. Donatelli, T. Suzuki, and Y. Kawaoka. Amino acid residues contributing to the substrate specificity of the influenza A neuraminidase. J Virol. 1999; 73: 6743~6751.
78. Koolen, M. J. M., M. A. J. Borst, M. C. Horzinek, and W. J. M. Spaan. 1990. Immunogenic peptide comprising a mouse hepatitis virus A59 B~cell epitope and an influenza virus T~cell epitope protects against lethal infection. J. Virol. 64:6270~6273.
79. Koopmans, M., B. Wilbrink, M. Conyn, G. Natrop, H. van der Nat, H. Vennema, A. Meijer, J. van Steenbergen, R. Fouchier, A. Osterhaus, A. Bosman. Transmission of H7N7 avian influenza A virus to human beings during a large outbreak in commercial poultry farms in the Netherlands. 2004. 363:587~593.
80. Krug, R. M. Priming of influenza viral RNA transcription by capped heterologous RNAs.Curr. Top. Microbiol. Immunol. 93:125~149.
81. Lee, C.W., D. A. Senne, and D. L. Suarez. (2004). Effect of vaccine use in the evolution of Mexican lineage H5N2 avian influenza virus. J. Virol. 78, 8372~8381.
82. Lee, C.~W., Song, C.~S., Lee, Y.~J., Mo, I.~P., Garcia, M., Suarez, D.L. & Kim, S.~J. (2000). Sequence analysis of the haemagglutinin gene of H9N2 Korean avian in?uenza viruses and assessment of the pathogenic potential of isolate MS96. Avian Diseases , 44 , 527~535.
83. Li, K. S., Xu, K. M., Peiris, J. S. M., Poon, L. L. M., Yu, K. Z., Yuen, K. Y., Shortridge, K. F., Webster, R. G., and Guan, Y. 2003. Characterization of H9 subtype influenza viruses from the ducks of southern China: a candidate for the next influenza pandemic in humans? J. Virol. 77, 6988~6994.
84. Li, S. Q., C. G. Liu, A. Klimov, K. Subbarao, M. L. Perdue, D. Mo, Y. L. Ji, L. Woods, S. Hietala, and M. Bryant. 1999. Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses.
85. Li, S., Polonis, V., Isobe, H., Zaghouani, H., Guinea, R., Morran, T., Bona, C., and Palese, P. 1993. Chimeric influenza virus induces neutralizing antibodies and cytotoxic T cells against human immunodeficiency virus type 1. J. Virol. 67: 6659~6666.
86. Lin, Y. P., Shaw, M., Gregory, V., Cameron, K., Lim, W., Klimov, A., Subbarao, K., Guan, Y. L., Krauss, S., Shortridge, K., Webster, R., Cox, N., and Hay, A. (2000). Avian~to~human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates. Proc. Natl. Acad. Sci USA 97, 9654~9658.
87. Liu, J. H., Okazaki, K., Shi, W. M., Wu, Q. M., Mweene, A. S., and Kida, H. (2003b). Phylogenetic analysis of neuraminidase gene of H9N2 influenza viruses prevalent in chickens in China during 1995~2002. Virus Genes. 27, 197~202.
88. Liu, J., Okazaki, K., Ozaki, H., Sakoda, Y., Wu, Q., Chen, F. & Kido, H. (2003). H9N2 in?uenza viruses prevalent in China are phylogenetically distinct from A/quail/Hong Kong/G1/97 presumed to be the donor of the internal protein genes of the H5N1 Hong Kong/97 virus. Avian Pathology, 32 , 551 /560.
89. Liu, M., He, S. Q., Walker, D., Zhou, N. N., Perez, D. R., Mo, B., Li, F., Huang, X. T.,Webster, R. G., and Webby, R. J. (2003). The influenza gene pool in a poultry market in south central China. Virology 305, 267~275.
90. Lu, J. H., X. F. Liu, W. X. Shao, P. H. Zhang, and D. P. Wei. 2003. Genetic Characterization of the Entire Genome of an H9N2 Avian Influenza Virus A/Chicken/Shanghai/F/98. Acta Microbiologica Sinica 43:434~441.
91. Lu, X. H., Renshaw, M., Tumpey, T. M., Kelly, G. D., Primmer, J. H., and Katz, J. M. (2001). Immunity to influenza A H9N2 viruses induced by infection and vaccination. J. Virol. 75, 4896~4901.
92. Lu, Y., M. Wambach, M. G. Katze, and R. M. Krug. Binding of the influenza virus NS1 protein to double~stranded RNA inhibits the activation of the protein kinase that phosphorylated the eIF~2 translation initiation factor. Virology 1995; 214: 222~228.
93. Ludwig, S., X. Wang, C. Ehrhardt, H. Zheng, N. Donelan, O. Planz, S. Pleschka, A. Garcia~Sastre, G. Heins, and T. Wolff. The influenza A virus NS1 protein inhibits activation of Jun N~terminal kinase and AP~1 transcription factors. J Virol 2002; 76: 11166~11171.
94. Luo, G., J. Chung, and P. Palese. Alterations of the stalk of the influenza virus neuraminidase: deletions and insertions. Virus Res 1993; 29: 141~153.
95. Luytjes, W., D. Geerts, W. Posthumus, R. Meloen, and W. Spaan. 1989. Amino acid sequence of a conserved neutralizing epitope of murine coronaviruses. J. Virol. 63:1408~1412.
96. Luytjes, W., L. S. Sturman, P. J. Bredenbeek, J. Charite, B. A. van der Zeijst, M. C. Horzinek, and W. J. Spaan. 1987. Primary structure of the glycoprotein E2 of coronavirus MHV~A59 and identification of the trypsin cleavage site. Virology 161:479~487.
97. Luytjes, W., M. Krystal, M. Enami, J. D. Pavin, and P. Palese. 1989. Amplification, expression and packaging of a foreign gene by influenza virus. Cell. 58:1107~1113.
98. Leong KH, Rainsay AJ, Boyle DB, Ramshaw IA. 1994. Selective induction of immune response by cytokines coexpressed in recombinant fowlpox virus. J Virol, 68: 8125~30.
99. Maassab, H. F., and Bryant, M. L. 1999. The development of live attenuated cold~adapted influenza virus vaccine for humans. Rev. Med. Virol. 9, 237~244.
100.Mase M, Imada T, Sanada Y, Etoh M, Sanada N, Tsukamoto K, Kawaoka Y, Yamaguchi S: Imported parakeets harbor H9N2 influenza A viruses that are genetically closely related to those transmitted to humans in Hong Kong. J Virol 75:3490~3494, 2001.
101.Matrosovich, M. N., S. Krauss, R.G. Webster. H9N2 influenza A viruses from poultry in Asia have human virus~like receptor specificity. Virology 2001; 281: 156~162.
102.Matrosovich, M., N. Tuzikov, A. Bovin, A. Gambaryan, A. Klimov, M.R. Castrucci, I. Donatelli, and Y. Kawaoka. Early alterations of the receptor~binding properties of H1, H2, H3 avian influenza virus haemagglutinins after their introduction into mammals. J Virol 2000; 74: 8502~8512.
103.Matrosovich,M., N. Zhou, Y. Kawaoka, and R. Webster. The surface glycoproteins of H5 influenza viruses from humans, chickens, and wild aquatic birds have distinguishable properties. J Virol 1999; 73: 1146~1155.
104. McKimm~Breschkin, J. L. 2000. Resistance of influenza viruses to neuraminidase inhibitors—a review. Antivir. Res. 47:1~17.
105.Mebatsion, T., M. J. Koolen, L. T. de Vaan, N. de Haas, M. Braber, A. Romer~Oberdorfer, P. van den Elzen, and P. van der Marel. 2002. Newcastle disease virus (NDV) marker vaccine: an immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope. J. Virol. 76:10138–10146.
106. Mebatsion, T., M. J. Koolen, L. T. de Vaan, N. de Haas, M. Braber, A. Romer~Oberdorfer, P. van den Elzen, and P. van der Marel. 2002. Newcastle disease virus (NDV) marker vaccine: an immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope. J. Virol. 76:10138~10146.
107. Mendel, D. B., C. Y. Tai, P. A. Escarpe, W. X. Li, R. W. Sidwell, J. H. Huffman, C. Sweet, K. J. Jakeman, J. Merson, S. A. Lacy, W. Lew, M. A. Williams, L. Zhang, M. S. Chen, N. Bishofberger, and C. U. Kim. 1998. Oral administration of a prodrug of the influenza virus neuraminidase inhibitor GS 4071 protects mice and ferrets against influenza infection. Antimicrob. Agents Chemother. 42:640~646
108. Mitnaul, L. J., Matrosovich, M. N., Castrucci, M. R., Tuzikov, A. B., Bovin, N. V., Kobasa, D.,and Kawaoka, Y. 2000. Balanced hemagglutinin and neuraminidase activitiesare critical for efficient replication of influenza A virus. J. Virol. 74:6015~6020.
109.Mo, I.P., Song, C.S., Kim, K.S. & Rhee, J.C. (1998). An occurrence of non~highly pathogenic avian in?uenza in Korea. In Proceedings of the 4th International Symposium on Avian In?uenza , 1997 (pp. 379~383). Athens, Georgia, USA.
110.Morris, S. J, G. E. Price, J. M. Barnett, S. A. Hiscox, H. Smith, and C. Sweet. Role of neuraminidase in influenza virus~induced apoptosis. J Gen Virol 1999; 80: 137~146.
111.Muster, T., Ferko, B., Klima, A., Purtscher, M., Trkola, A., Schulz, P., Grassauer, A., Engelhardt, O.G., Garcia~Sastre, A., Palese, P., and Katinger, H. 1995. Mucosal model of immunization against human immunodeficiency virus type 1 with a chimeric influenza virus. J. Virol. 69, 6678~6686.
112.Muster, T., Guinea, R., Trkola, A., Purtscher, M., Klima, A., Steindl, F., Palese, P., and Katinger, H. 1994. Cross~neutralizing activity against divergent human immunodeficiency virus type 1 isolates induced by the gp41sequence ELDKWAS. J. Virol. 68: 4031~4034.
113.Naeem, K., Naurin, M., Rashid, S. & Bano, S. (2003). Seroprevalence of avian in?uenza virus and its relationship with increased mortality and decreased egg production. Avian Pathology, 32 , 283~287.
114.Naeem, K., Ullah, A., Manvell, R.J.& Alexander, D.J. (1999). Avian in?uenza A subtype H9N2 in poultry in Pakistan. Veterinary Record , 145 , 560.
115. Naffakh, N., P. Massin, N. Escriou, B. Crescenzo~Chaigne, and S. van der Werf. 2000. Genetic analysis of the compatibility between olymerase proteins from humans and avian strains of influenza A viruses. J. Gen. Virol. 81:1283~1291.
116.Neumann, G., A. Zobel, and G. Hobom. 1994. RNA polymerase I~mediated expression of influenza viral RNA molecules. Virology 202:477~479.
117.Neumann, G., Hughes, M. T., and Kawaoka, Y. Influenza A virus NS2 protein mediates Vrnp nuclear export through NES~independent interactionwith hCRM1. EMBO J. 2000,19:6751~6758.
118.Neumann, G., Watanabe, T., and Kawaoka, Y. Plasmid~driven formation of influenza virus~like particles. J. Virol. 2000,547~551.
119.Neumann, G., Watanabe, T., Ito, H., Watanabe, S., Goto, H., Gao, P., Hughes, M., Perez, D., Donis, R., Hoffmann, E., Hobom, G., and Kawaka, . 1999. Generation of influenza A viruses entirely from cloned cDNAs. Proc. Natl. Acad. Sci. 96, 9345~9350.
120.Nili, H. & Asasi, K. (2002). Natural cases and an experimental study of H9N2 avian in?uenza in commercial broiler chickens of Iran. Avian Pathology, 31:247~252.
121.Nili, H. & Asasi, K. (2003). Avian In?uenza (H9N2) outbreak in Iran. In: Proceedings of the Fifth International Symposium on Avian In?uenza, Athens, Georgia, April 14~17 2002. Avian Diseases , 47 , 828~831.
122.Nobusawa, E., T. Aoyama, H. Kato, Y. Suzuki, Y. Tateno, and K. Nakajima. Comparison of complete amino acid sequences and receptor~binding properties among 13 serotypes of haemagglutinins of influenza A viruses. Virology .1991,182: 475~485.
123.Ohuchi, M., M. Orlich, R. Ohuchi, B. E. Simpson, W. Garten, H. D. Klenk, and R. Rott. Mutations at the cleavage site of the haemagglutinin alter the pathogenicity of influenza virus A/Chick/Penn/83 [H5N2]. Virology .1989; 168: 274~280.
124. Ohuchi, M., Orlich, M., Ohuchi, R., Simpson, B. E., Garten, W., Klenk, H. D., and Rott, R. Mutations at the cleavage site of the hemagglutinin alter the pathogenicity of influenza virus A/Chick/Penn/83(H5N2). Virology 168:274~280.
125.Ohuchi, M., R. Ohuchi, A. Feldmann, and H. K. Klenk. Regulation of receptor binding affinity of influenza virus haemagglutinin by its carbohydrate moiety. J Virol 1997,71: 8377~8384.
126. Ohuchi, R., Ohuchi, M., Garten, W., and Klenk, H. D. Oligo~saccharides in the stem region maintain the influenza virus hemagglutinin in the metastable forn required for fusion activity. 1997. J. Virol. 71:3719~3725.
127.Okazaki, K., Y. Kawaoka, R. G. Webster. Evolutionary pathways of the PA genes of influenza A viruses. Virology 1989. 172: 601~608.
128.O'Neill, E., S. L. Krauss, J. M. Riberdy, R. G. Webster, and D. L. Woodland. Heterologous protection against lethal A/HongKong/156/97 (H5N1) influenza virus infection in C57BL/6 mice. J Gen Virol 2000; 81: 2689~2696.
129.Ozaki, H., Govorkova, E. A., Li,C. Xiong, X., Webster, R. G., and Webby, R. J.Generation of high~yielding influenza A viruses in African green monkey kidney cells by reverse genetics. J. Virol. 2004, 78, 1851~1857.
130.Parvin, J. D., P. Palese, A. Honda, A. Ishihama, and M. Krystal. Promoter analysis of influenza virus RNA polymerase. J. Virol. 1989,68:5142~5152.
131.陈化兰,于康震,田国斌等.DNA免疫诱导鸡对禽流感病毒的免疫保护反应.中国农业科学,1998,31:63~68
132.陈化兰,于康震,田国斌等. H5和H7亚型禽流感病毒HA基因的RT~PCR扩增及克隆.中国畜禽传染病,1997,2: 160~180.
133.陈化兰,于康震,田国斌等.禽流感病毒血凝素基因的克隆及其DNA疫苗的免疫源性研究 ,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编,中国农业科技出版社).1998,5: 356~3590.
134.李海燕,辛晓光,田国斌等.禽流感抗体间接酶联免疫吸附试验检测法的建立.中国畜禽传染病,1998,20增刊:157~159.
135.陈伯伦,张泽纪,陈伟斌.关于禽流感几个问题的看法.中国畜禽传染病,1998,20增刊 : 230~231.
136.陈伯伦,张泽纪,陈伟斌.当前防制禽流感的建议.中国畜禽传染病,1998,20增刊:2 31 ~233.
137.陈化兰,于康震,卢景良.禽流感病毒及其分子生物学研究进展.畜禽重大疫病生物技术防制研究(白新盛,卢景良主编).中国农业科技出版社,1998,5:313~320.
138.李海燕,于康震,张晶.禽流感诊断技术研究进展,畜禽重大疫病生物技术防制研究.中国农业科技出版社,1998,5:329~334.
139.陈化兰,于康震.基因免疫研究进展,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编.中国农业科技出版社,1998,5:325~328.
140.崔尚金,陈化兰,邓国华等.禽流感RT~PCR诊断法的建立,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编).中国农业科技出版社1998,5:329~334.
141.邓国华,于康震.H5亚型禽流感病毒核蛋白基因的扩增与克隆,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编).中国农业科技出版社,1998,5:348~351.
142.田国斌,唐秀英,于康震.禽流感病毒感染鸡抗体的监测及血凝素分型血清的制备,畜禽重大疫病生物技术防制研究(白新盛,卢景良主编).中国农业科技出版社,1998,5:352~335.
143.田国斌,赵增连,唐秀英等.鸭源禽流感病毒的分离和鉴定.中国预防兽医学报, 1999,21(1):1 ~3.
144.邓国华,马文军,于康震.禽流感病毒核蛋白基因在杆状病毒中的表达.中国预防兽医学报,1999,21(2):81~ 83.
145.刘明.H5和H7亚型禽流感分子防制技术的研究[博士论文].中国农业科学院哈尔滨兽医研究所,2000,6.
146.张建林.禽流感病毒A/Goose/GuangDONG/1/96( H5Nl) 分离株全基因序列分析[硕士论文].中国农业科学院哈尔滨兽医研究所,2000,6.
147.李一经,刘胜旺,陈立君等.马立克氏病弱毒苗接种鸡T细胞亚群观察.中国畜禽 传染病,1997,20( 6):345~347.
148.李一经,刘胜旺,陈立君等.马立克氏病毒强毒株感染鸡T细胞表型动态变化的研究 .上海免疫学杂志,1998,18(5):308~309,311.
149.贾永清,陈化兰,邓国华等.禽流感病毒分离株A/Goose/Guangdong/3/96(H 5N 1) N A 基因序列分析.中国预防兽医学报,2000,22 (1):25~30.
150.乔传玲.表达禽流感病毒HA~NA、HA~NP及NP基因重组禽痘病毒的构建及其免疫效力的研究[博士论文].中国农业科学院哈尔滨兽医研究所,2002,6.
151.刘胜旺,李一经,曹殿军等.新城疫油苗免疫鸡血清1g抗体含量及T细胞亚类的观察.中国免疫学杂志,1999,14:65~67.
152.毕英佐,曹永长,朱基美.东禽流感的血清学调查.中国兽医杂志,1994, 20(7): 8–9.
153.程安春,汪铭书,廖德惠等.鸡A型流感病毒的分离及初步鉴定.四川畜牧兽医,1995,4:5~6.
154.陈伯伦,张泽纪,陈伟斌.禽流感研究鸡A型流感病毒的分离与血清学初步鉴定 . 中国兽医杂志,1994,2(10):3~7.
155.郭元吉,范瑞莲,曲风珍等.甲型流感病毒在我国动物中分布的初步调查.微生物学报,1981,21(3):379~384.
156.马文军.禽流感病毒血凝素基因重组禽痘病毒的构建[硕士研究论文].中国农业科学院哈尔滨兽医研究,1999.
157.甘孟侯.禽流感[M].第 2 版,北京:中国农业出版社.2002,98~100.
158.郭元吉,李建国,程小雯,等.禽 H9N2 亚型流感病毒能感染人的发现.中国实验和临床病毒学杂志,1999,13(2):105~108.
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