果寡糖在肉仔鸡饲料中的应用研究
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摘要
试验分为两期进行，探讨了不同果寡糖添加水平对雏鸡鼠伤寒沙门氏菌感染的控制
效应以及果寡糖、益生素、抗生素对肉仔鸡生产性能、肠道微生态、营养物质利用率的
影响。
一期试验：选择96只艾维茵肉仔鸡随机均分4组，每组尽个重复，研究了在基础日
粮（不添加任何药物）中添加0.5%、1.1%、2.2%果寡糖对雏鸡鼠伤寒沙门氏菌感染的抑
制效果。试验期从１日龄开始，21日龄结束。16日龄将所有鸡滴口进行 10~8鼠伤寒沙门
氏菌攻毒，分别于17，19，21日龄每组抽取4只鸡，颈部错位致死后取盲肠进行沙门
氏菌的分离培养及 pH值、盲肠重的测定。试验结果表明：19日龄的测定结果中，0.5％、
1.0%、2.0%的果寡糖添加均能显著（＜0.05）降低盲肠内沙门氏菌数量，分别使菌数减少
56.28%、56.52%、57.73%;果寡糖不同处理间无显著差异（P＞0.05)。17日龄测定结果
中各果寡糖组试验鸡盲肠沙门氏菌数分别较对照组降低T54．49%（p＞0．05）、52．99%（p
＞0.05)、 45.69%（P＞0.05%)。饲粮中添加果寡糖有增加肉仔鸡盲肠重的趋势，但对 pH
无影响。
二期试验：192只艾维茵肉仔鸡随机均分4组，每组设4个重复，对照组（Ⅰ）喂给基
础日粮（不添加任何药物),试验组(Ⅱ、Ⅲ、Ⅳ）分别在基础日粮中添加1.5%果寡糖、
800mg/kg生素、300mg/kg杆菌肽锌。从1日龄开始进行为期49天的饲养试验。每周测
定各组试验鸡的体重、结算饲料消耗及饲料转化率。分别于14日龄、21日龄、35日龄、
49日龄每组取4只鸡屠宰取盲肠进行肠道菌群、pH、水分的测定。于50日龄开始进行为
期3天的代谢试验。试验结果表明：饲粮中添加 1.5%的果寡糖显著降低了（P＜0.05）肉
鸡的日增重及出栏体重，与对照组相比，日增重、49日龄出栏体重分别降低了7.83％（p＜
0.05）、7.67%（P＜0.05）。各组肉鸡日增重分别为：45.75g、42.17g、45.42g和46.66g；
49日龄出栏体重各组分别为2285.94g、2110.62g、2269.75g和2330.85g。全期采食量、料
重比各组之间统计上均差异不显著（P＞0.05),但果寡糖组肉鸡采食量较低，分别比对
照组、益生素组、杆菌肽锌组低4.28%、3.39%、5.48%;全期耗料增重比也分别较对照
组、益生素组、杆菌肽锌组高3.98%、3.98%、4.50%。杆菌肽锌的添加对肉鸡的增重及
饲料转化率略有改善，提高了肉鸡增重1.99％，但差异不明显。益生素的添加没有表现
出较好的促生长效果，与对照组相比无显著差异。果寡糖的添加能显著地提高肉仔鸡14
日龄、21日龄盲肠中双歧杆菌的数量，与对照组相比，分别增加了1.75倍（P＜0.05)、
l.45倍（P＜0.05). 21日龄后，各组肠道双歧杆菌数量差异不显著。果寡糖对盲肠内容
物的pH、含水量及营养物质利用率均无显著影响（P＞0.05）。
Two experiments were conducted to Study the effect of the different supplement level of
FOS on the control of potential escalation of Salmonella typhimruium colonization druing
young chickens, and the effects of FOS, PS(probiotics) and Zinc bacitracin on performance,
intestinal bacteria population and availability of nutrition.
In expement l:96 broilers randomly assigned to four groups, with four replicaions per
group. The control group was provided with the basal diet. FOS was added to the basal diet at
the level of 0.5%, l.0% and 2.0% respectively Chickens were challenged per os at day 16 by
providing water containing 10~8 S.typhimurium per milliliter At day l7,l9 and 2l, the cecal
contents were examined for S.typhimurium, pH and cecal weight. The result showed that 0.5%,
l.0% and 2.0% FOS bouced significantly the population of Salmonella by 56.28%(P<0.05),
56.52%(P<0.05) and 57.73%(P<0.05) respectively at day l9 and 54.49%(P >0.05), 52.99%
(P>0.05) and 45.69%(P>0.05) respectively at day l7. There was no different among
treatments of FOS. FOS made cecal weights have an increasing tendency, but did not affect
pH.
In experiment 2:l92 broilers were randomly assigned to four groups, with four
replications per group. The control group(Ⅰ) was fed with the basal diet (not including any
drug additive ). FOS(Ⅱ),DFM(Ⅲ)and Zinc bacitracin(Ⅳ) was respectively added to the basal
diet at the level of 1.5%,800mg/kg and 300mg/kg to form four experiment diets. Weight, feed
intake and feed efficiency were measured. The feeding trial starts at 1d and ends at 49d. At
day l4,2l,35 and 49, four broilers per group were killed and removed the cecum to test
bacteria, pH and moisture contnt. Metabolic trial was done at 50d of age. The rosult showed
that the dietaly FOS inhibits growth significanly(P<0.05 ).Daily weigh gain cach group was
respectively 45.75g, 42.l7g, 45.42g and 46.66g. The average weigh of 49d was 2285.94g,
2110.62g 2269.75g and 2330.85g Wively FOS decreased daily weight gain and the
weight of 49d by 7.83% (P<0.05 ) and 7.67% (P<0.05 ) feed intake and feed to gain were no
different among poups (P>0.05),but feed intak of the FOS group was dectrased
4.28%,3.39% and 5.48% respectively,feed to gain was increased 3.98%,3.98% and 4.50%
respectiveyly, when compared with the control, PS and Zinc bacitracin Zinc bacitracin
improved they weight gain by l.99% but the effect is not apparent.The performance was
unaffected by supplement of PS. The dietary FOS increased Bifidobacterium concentration by
！．75－fold（P＜0．05）at 14d of4eand！．45－fold（P＜0．05）at21dofqe．FOShasnoeffect
on pH，moisture content and availability ofnutrition（P＞0．05）．
Master Candidate：Wa回g Ling
supervsosor：Pro【s回玉n AllS回a回
地jor： Animal Nutrition and F础 Science
效应以及果寡糖、益生素、抗生素对肉仔鸡生产性能、肠道微生态、营养物质利用率的
影响。
一期试验：选择96只艾维茵肉仔鸡随机均分4组，每组尽个重复，研究了在基础日
粮（不添加任何药物）中添加0.5%、1.1%、2.2%果寡糖对雏鸡鼠伤寒沙门氏菌感染的抑
制效果。试验期从１日龄开始，21日龄结束。16日龄将所有鸡滴口进行 10~8鼠伤寒沙门
氏菌攻毒，分别于17，19，21日龄每组抽取4只鸡，颈部错位致死后取盲肠进行沙门
氏菌的分离培养及 pH值、盲肠重的测定。试验结果表明：19日龄的测定结果中，0.5％、
1.0%、2.0%的果寡糖添加均能显著（＜0.05）降低盲肠内沙门氏菌数量，分别使菌数减少
56.28%、56.52%、57.73%;果寡糖不同处理间无显著差异（P＞0.05)。17日龄测定结果
中各果寡糖组试验鸡盲肠沙门氏菌数分别较对照组降低T54．49%（p＞0．05）、52．99%（p
＞0.05)、 45.69%（P＞0.05%)。饲粮中添加果寡糖有增加肉仔鸡盲肠重的趋势，但对 pH
无影响。
二期试验：192只艾维茵肉仔鸡随机均分4组，每组设4个重复，对照组（Ⅰ）喂给基
础日粮（不添加任何药物),试验组(Ⅱ、Ⅲ、Ⅳ）分别在基础日粮中添加1.5%果寡糖、
800mg/kg生素、300mg/kg杆菌肽锌。从1日龄开始进行为期49天的饲养试验。每周测
定各组试验鸡的体重、结算饲料消耗及饲料转化率。分别于14日龄、21日龄、35日龄、
49日龄每组取4只鸡屠宰取盲肠进行肠道菌群、pH、水分的测定。于50日龄开始进行为
期3天的代谢试验。试验结果表明：饲粮中添加 1.5%的果寡糖显著降低了（P＜0.05）肉
鸡的日增重及出栏体重，与对照组相比，日增重、49日龄出栏体重分别降低了7.83％（p＜
0.05）、7.67%（P＜0.05）。各组肉鸡日增重分别为：45.75g、42.17g、45.42g和46.66g；
49日龄出栏体重各组分别为2285.94g、2110.62g、2269.75g和2330.85g。全期采食量、料
重比各组之间统计上均差异不显著（P＞0.05),但果寡糖组肉鸡采食量较低，分别比对
照组、益生素组、杆菌肽锌组低4.28%、3.39%、5.48%;全期耗料增重比也分别较对照
组、益生素组、杆菌肽锌组高3.98%、3.98%、4.50%。杆菌肽锌的添加对肉鸡的增重及
饲料转化率略有改善，提高了肉鸡增重1.99％，但差异不明显。益生素的添加没有表现
出较好的促生长效果，与对照组相比无显著差异。果寡糖的添加能显著地提高肉仔鸡14
日龄、21日龄盲肠中双歧杆菌的数量，与对照组相比，分别增加了1.75倍（P＜0.05)、
l.45倍（P＜0.05). 21日龄后，各组肠道双歧杆菌数量差异不显著。果寡糖对盲肠内容
物的pH、含水量及营养物质利用率均无显著影响（P＞0.05）。
Two experiments were conducted to Study the effect of the different supplement level of
FOS on the control of potential escalation of Salmonella typhimruium colonization druing
young chickens, and the effects of FOS, PS(probiotics) and Zinc bacitracin on performance,
intestinal bacteria population and availability of nutrition.
In expement l:96 broilers randomly assigned to four groups, with four replicaions per
group. The control group was provided with the basal diet. FOS was added to the basal diet at
the level of 0.5%, l.0% and 2.0% respectively Chickens were challenged per os at day 16 by
providing water containing 10~8 S.typhimurium per milliliter At day l7,l9 and 2l, the cecal
contents were examined for S.typhimurium, pH and cecal weight. The result showed that 0.5%,
l.0% and 2.0% FOS bouced significantly the population of Salmonella by 56.28%(P<0.05),
56.52%(P<0.05) and 57.73%(P<0.05) respectively at day l9 and 54.49%(P >0.05), 52.99%
(P>0.05) and 45.69%(P>0.05) respectively at day l7. There was no different among
treatments of FOS. FOS made cecal weights have an increasing tendency, but did not affect
pH.
In experiment 2:l92 broilers were randomly assigned to four groups, with four
replications per group. The control group(Ⅰ) was fed with the basal diet (not including any
drug additive ). FOS(Ⅱ),DFM(Ⅲ)and Zinc bacitracin(Ⅳ) was respectively added to the basal
diet at the level of 1.5%,800mg/kg and 300mg/kg to form four experiment diets. Weight, feed
intake and feed efficiency were measured. The feeding trial starts at 1d and ends at 49d. At
day l4,2l,35 and 49, four broilers per group were killed and removed the cecum to test
bacteria, pH and moisture contnt. Metabolic trial was done at 50d of age. The rosult showed
that the dietaly FOS inhibits growth significanly(P<0.05 ).Daily weigh gain cach group was
respectively 45.75g, 42.l7g, 45.42g and 46.66g. The average weigh of 49d was 2285.94g,
2110.62g 2269.75g and 2330.85g Wively FOS decreased daily weight gain and the
weight of 49d by 7.83% (P<0.05 ) and 7.67% (P<0.05 ) feed intake and feed to gain were no
different among poups (P>0.05),but feed intak of the FOS group was dectrased
4.28%,3.39% and 5.48% respectively,feed to gain was increased 3.98%,3.98% and 4.50%
respectiveyly, when compared with the control, PS and Zinc bacitracin Zinc bacitracin
improved they weight gain by l.99% but the effect is not apparent.The performance was
unaffected by supplement of PS. The dietary FOS increased Bifidobacterium concentration by
！．75－fold（P＜0．05）at 14d of4eand！．45－fold（P＜0．05）at21dofqe．FOShasnoeffect
on pH，moisture content and availability ofnutrition（P＞0．05）．
Master Candidate：Wa回g Ling
supervsosor：Pro【s回玉n AllS回a回
地jor： Animal Nutrition and F础 Science
引文
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3．蔡永峰 袁德轩 杆菌肽锌在畜禽养殖中的应用及其安全性 国外畜牧科技 2000 (4)：35-37
4．杨曙明 寡糖在动物营养研究中的进展 动物营养学报 1999，11 (1)：1-9
5．余东游 李卫芬 寡聚糖在饲料工业中的研究和应用进展 天然产物研究与开发 1999，11 (4) 81-84
6．杨全明 张均利 益康素对蛋鸡公雏肠道微生物数量的影响研究 饲料研究 1999，8：28-29
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9．周中凯 新型饲料添加剂-功能性低聚糖 广东饲料 1999，1：29-31
10．王亚军 吴天星 果寡糖及其在饲料工业中的应用 饲料研究 1999，12：2-7
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13．姚建国 一种新型的饲料添加剂—低聚果糖 饲料博览 2000，5：37-39
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22．石宝明 寡聚糖在仔猪饲料中的应用研究2000东北农业大学硕士学位论文
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