豌豆种质资源遗传多样性分析及白粉病抗性评价
|
摘要
豌豆(Pisum sativum L.)是重要的粮菜饲兼用作物,具有耐寒、耐旱、耐瘠等特点,因其适应能力很强,在全世界范围内都有广泛的地理分布,广阔的分布范围和多样化的生境条件形成了其种质资源丰富的遗传多样性。由豌豆白粉菌(Erysiphe pisi)引起的豌豆白粉病是最严重的豌豆病害之一,抗病品种选育是防治其病害发生的最经济有效的措施,品种的抗病性鉴定是抗病育种的基础。本研究在对豌豆种质资源表型性状进行调查及测算的基础上,通过统计学分析手段,对其表型性状遗传多样性进行了研究,并进一步应用ISSR标记技术,从DNA水平上探讨了豌豆种质资源的遗传多样性与亲缘关系。同时,对豌豆材料白粉病田间自然发病情况做了详细调查,并对初步筛选出的抗病材料进一步进行了室内人工接种鉴定,在此基础上对豌豆白粉病不同抗性品种接种前后的生理生化变化进行了研究。主要的研究结果如下：
1、通过对120份豌豆材料的20个表型性状分析,结果表明：豌豆属植物种质资源形态变异十分丰富,平均变异系数为32.68%,平均多样性指数为1.071。方差分析结果显示出供试材料种间存在显著或极显著差异,国内和国外群体内差异显著。主成分分析表明,前6个主成分的累积贡献率达到80.369%。120份材料在欧式遗传距离为56.59处聚为2大类,第1类主要为北方材料,第2类主要为南方材料,但是也有个别材料交叉出现,其聚类图位置依然较近,这说明系统聚类结果受材料的地理来源影响较大。
2、建立了豌豆属ISSR分析的技术体系,对影响其ISSR-PCR反应体系中的5种主要因素进行了优化,最终确定了豌豆ISSR-PCR反应的最佳体系(20μL)为：15ng模板DNA,0.15mmol/LdNTP,0.5μmol/L ISSR引物,1U Taq DNA聚合酶,引物842号的最适退火温度为50℃。反应程序为：94℃充分变性3min,然后进行以下34个循环：94℃变性30s,50℃退火30s,72℃延伸45s,最后于72℃延伸10min,4℃终止反应保存。
3、用ISSR标记技术对来自国内外的73份豌豆材料进行遗传多样性分析,结果表明：100个ISSR引物中共筛选出11个多态性明显、条带清晰、反应稳定的引物,73份材料DNA共扩增出91条条带,其中78条为多态性条带,平均每个引物扩增的条带数为8.2条,多态性比率为86.4%。Shannon多样性指数平均为0.4202,每个位点的有效等位基因数为1.4518,品种间遗传相似系数变幅范围为0.4065-0.9340,表现出丰富的遗传多样性。利用UPGMA聚类分析,以遗传相似系数0.52为界限,73份材料划分为5类,聚类基本符合地理来源相近的材料聚为一类,呈现出一定的地域性分布规律。
4、通过2009-2011年连续3年对来自国内外的535份豌豆品种资源的白粉病田间自然鉴定,结果表明：田间表现高抗的有1个品种,占鉴定总数的0.19%；抗病品种2个,占总数的0.37%；中抗品种17个,占总数的3.18%；中感品种60个,占总数的11.12%；感病品种434个,占总数的81.12%；高感品种21个,占总数的3.93%。通过对田间表现出抗性的20个品种进行室内苗期接种鉴定表明,除了4个中抗品种的抗性发生了变化外,其余均与田间抗性表现一致,具有较好的抗性稳定性。
5、通过对豌豆白粉病不同抗性品种接种前后的抗性相关酶活性变化及可溶性糖、可溶性蛋白和叶绿素含量变化的研究,结果表明：豌豆白粉病抗性反应相关防御酶中,苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)及过氧化氢酶(CAT)的活性变化与豌豆白粉病抗性之间存在明显的正相关性,过氧化物酶(POD及多酚氧化酶(PPO)与豌豆白粉病抗性之间无明显的相关性。接种后,抗病品种中PAL、SOD和CAT3种酶活性明显大于感病品种。与抗性相关的物质中,可溶性蛋白和叶绿素的含量与品种抗病性之间存在明显的正相关,可溶性糖的含量与品种抗病性呈负相关。接种后,抗病品种中可溶性蛋白和叶绿素的含量明显高于感病品种。
The pea(Pisum sativum L.) was an important food, vegetable and feeding mixture-purposed crop, with cold and drought and barren tolerant or other characteristics, it had the wide distribution in the world because of its strong ability to adapt, the broad geographical distribution and diversification of habitat conditions forming rich genetic diversity. The powdery mildew of pea caused by Erysiphe pisi was the one of the most serious diseases of pea, the most economic and effective measures of prevention and control the disease was selecting resistant varieties, the varieties resistance identification was the basic of selecting resistant varieties. The study on the basic of investigation and measure the pea germplasm resources phenotypic character, through the statistical analysis method, research the genetic diversity of traits, and further application ISSR markers, discuss the pea germplasm resources of genetic diversity and paternity. At the same time, make a detailed investigation of pea's powdery mildew in the field on natural, and take the further indoor inoculating artificially identification to the resistance materials, on this basis, research the physiological changes of different varieties of powdery mildew resistance pea before and after inoculated. The main research results were as follows:
1. Through analysis to20phenotypes of120pea materials, the results showed that:the pea germplasm resources variation form was very rich, the average coefficient of variation was32.68%, and the average diversity index was1.071. The results of variance analysis showed that the material had significant or very significant difference, the domestic and foreign community significantly different. Principal component analysis showed that, the first six main composition accumulation contribution rate reached80.369%.120material place for2categories in genetic distance of56.59, the clustering results divided south and north materials into2kinds, one kind of main materials for the north, the second type of main materials for the south, but also have individual materials cross appear, its clustering figure position remains relatively close, it showed that system clustering results from the geographical source material had big effect.
2. Set up the technical analysis of pea ISSR system, optimized ISSR-PCR amplification system on pea in five factors. The most suitable ISSR-PCR system for pea was established, namely20μL reaction system containing15ng DNA template,0.15mmol/L dNTP,0.5μmol/L ISSR primer,1U Taq DNA Polymerase, the suitable annealing temperature of primer842is50℃. Amplificative thermal reaction program suitable for the ISSR-PCR analysis in the Pisum sativum was devised as followings:one preliminary denaturation at94℃for3min;34cycles each involved at94℃for30s, anneal at50℃for30s, extended at72℃for45s; and a final extension at72℃for10min, and keeping final products at4℃
3. The genetic diversity of the73materials of Pisum sativum from both at home and abroad were analyed by ISSR marker, the results showed that:11primers were selected from100primers which have the clearly and polymorphic bands, a total of91bands were obtained by73materials, including78polymorphism bands.On average, each primer amplification site for8.2and the polymorphism percentage was86.4%. Shannon's index was0.4202in average and the number of effective alleles was1.4518, the genetic similarity coefficient ranged from0.4065to0.9340and showed rich genetic diversity. Use groups by UPGMA cluster analysis to the genetic similarity coefficient0.52for boundaries,73materials was divided into five types, clustering basic accord with geographical origin of close materials for a class together, has a certain regional distribution.
4. In order to make full use of disease-resistant peas germplasm resources, the study tested the resistance of pea to powdery mildew in field natural of535pea resources from international and domestic in2009-2011.The result showed that one variety was high resistant, accounting for0.19%of the total number of appraisal; two varieties showed resistance, accounting for0.37%of the total;17varieties showed middle resistance, accounting for3.18%;60varieties showed middle susceptible, accounting for11.12%;434varieties showed susceptible, accounting for81.12%;21varieties showed high susceptible, accounting for3.93%. The resistance indoor was consistent with it in field except4middle resistance varieties through the indoor inoculation identification of20varieties which have showed resistance in field.
5. Through to research the resistance related enzyme activity change and soluble sugar, soluble protein and chlorophyll content change of different resistant varieties of pea powdery mildew before and after inoculation, the results showed that:in pea's powdery mildew resisitance response enzyme which related defense, PAL, SOD and CAT activities change had obviously positive correlation with pea's powdery mildew resistance, POD and PPO had no obvious correlation with pea's powdery mildew. After inoculation, the PAL, SOD and CAT in disease-resistant varieties had significant enzyme activity greater than susceptible varieties. In related resistance material, soluble protein and chlorophyll content had obviously positive correlation with variety resistance, soluble sugar content had negatively correlated with variety resistance. After inoculation, disease-resistant varieties of soluble protein and the chlorophyll content was significantly higher than the susceptible varieties.
1、通过对120份豌豆材料的20个表型性状分析,结果表明：豌豆属植物种质资源形态变异十分丰富,平均变异系数为32.68%,平均多样性指数为1.071。方差分析结果显示出供试材料种间存在显著或极显著差异,国内和国外群体内差异显著。主成分分析表明,前6个主成分的累积贡献率达到80.369%。120份材料在欧式遗传距离为56.59处聚为2大类,第1类主要为北方材料,第2类主要为南方材料,但是也有个别材料交叉出现,其聚类图位置依然较近,这说明系统聚类结果受材料的地理来源影响较大。
2、建立了豌豆属ISSR分析的技术体系,对影响其ISSR-PCR反应体系中的5种主要因素进行了优化,最终确定了豌豆ISSR-PCR反应的最佳体系(20μL)为：15ng模板DNA,0.15mmol/LdNTP,0.5μmol/L ISSR引物,1U Taq DNA聚合酶,引物842号的最适退火温度为50℃。反应程序为：94℃充分变性3min,然后进行以下34个循环：94℃变性30s,50℃退火30s,72℃延伸45s,最后于72℃延伸10min,4℃终止反应保存。
3、用ISSR标记技术对来自国内外的73份豌豆材料进行遗传多样性分析,结果表明：100个ISSR引物中共筛选出11个多态性明显、条带清晰、反应稳定的引物,73份材料DNA共扩增出91条条带,其中78条为多态性条带,平均每个引物扩增的条带数为8.2条,多态性比率为86.4%。Shannon多样性指数平均为0.4202,每个位点的有效等位基因数为1.4518,品种间遗传相似系数变幅范围为0.4065-0.9340,表现出丰富的遗传多样性。利用UPGMA聚类分析,以遗传相似系数0.52为界限,73份材料划分为5类,聚类基本符合地理来源相近的材料聚为一类,呈现出一定的地域性分布规律。
4、通过2009-2011年连续3年对来自国内外的535份豌豆品种资源的白粉病田间自然鉴定,结果表明：田间表现高抗的有1个品种,占鉴定总数的0.19%；抗病品种2个,占总数的0.37%；中抗品种17个,占总数的3.18%；中感品种60个,占总数的11.12%；感病品种434个,占总数的81.12%；高感品种21个,占总数的3.93%。通过对田间表现出抗性的20个品种进行室内苗期接种鉴定表明,除了4个中抗品种的抗性发生了变化外,其余均与田间抗性表现一致,具有较好的抗性稳定性。
5、通过对豌豆白粉病不同抗性品种接种前后的抗性相关酶活性变化及可溶性糖、可溶性蛋白和叶绿素含量变化的研究,结果表明：豌豆白粉病抗性反应相关防御酶中,苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)及过氧化氢酶(CAT)的活性变化与豌豆白粉病抗性之间存在明显的正相关性,过氧化物酶(POD及多酚氧化酶(PPO)与豌豆白粉病抗性之间无明显的相关性。接种后,抗病品种中PAL、SOD和CAT3种酶活性明显大于感病品种。与抗性相关的物质中,可溶性蛋白和叶绿素的含量与品种抗病性之间存在明显的正相关,可溶性糖的含量与品种抗病性呈负相关。接种后,抗病品种中可溶性蛋白和叶绿素的含量明显高于感病品种。
The pea(Pisum sativum L.) was an important food, vegetable and feeding mixture-purposed crop, with cold and drought and barren tolerant or other characteristics, it had the wide distribution in the world because of its strong ability to adapt, the broad geographical distribution and diversification of habitat conditions forming rich genetic diversity. The powdery mildew of pea caused by Erysiphe pisi was the one of the most serious diseases of pea, the most economic and effective measures of prevention and control the disease was selecting resistant varieties, the varieties resistance identification was the basic of selecting resistant varieties. The study on the basic of investigation and measure the pea germplasm resources phenotypic character, through the statistical analysis method, research the genetic diversity of traits, and further application ISSR markers, discuss the pea germplasm resources of genetic diversity and paternity. At the same time, make a detailed investigation of pea's powdery mildew in the field on natural, and take the further indoor inoculating artificially identification to the resistance materials, on this basis, research the physiological changes of different varieties of powdery mildew resistance pea before and after inoculated. The main research results were as follows:
1. Through analysis to20phenotypes of120pea materials, the results showed that:the pea germplasm resources variation form was very rich, the average coefficient of variation was32.68%, and the average diversity index was1.071. The results of variance analysis showed that the material had significant or very significant difference, the domestic and foreign community significantly different. Principal component analysis showed that, the first six main composition accumulation contribution rate reached80.369%.120material place for2categories in genetic distance of56.59, the clustering results divided south and north materials into2kinds, one kind of main materials for the north, the second type of main materials for the south, but also have individual materials cross appear, its clustering figure position remains relatively close, it showed that system clustering results from the geographical source material had big effect.
2. Set up the technical analysis of pea ISSR system, optimized ISSR-PCR amplification system on pea in five factors. The most suitable ISSR-PCR system for pea was established, namely20μL reaction system containing15ng DNA template,0.15mmol/L dNTP,0.5μmol/L ISSR primer,1U Taq DNA Polymerase, the suitable annealing temperature of primer842is50℃. Amplificative thermal reaction program suitable for the ISSR-PCR analysis in the Pisum sativum was devised as followings:one preliminary denaturation at94℃for3min;34cycles each involved at94℃for30s, anneal at50℃for30s, extended at72℃for45s; and a final extension at72℃for10min, and keeping final products at4℃
3. The genetic diversity of the73materials of Pisum sativum from both at home and abroad were analyed by ISSR marker, the results showed that:11primers were selected from100primers which have the clearly and polymorphic bands, a total of91bands were obtained by73materials, including78polymorphism bands.On average, each primer amplification site for8.2and the polymorphism percentage was86.4%. Shannon's index was0.4202in average and the number of effective alleles was1.4518, the genetic similarity coefficient ranged from0.4065to0.9340and showed rich genetic diversity. Use groups by UPGMA cluster analysis to the genetic similarity coefficient0.52for boundaries,73materials was divided into five types, clustering basic accord with geographical origin of close materials for a class together, has a certain regional distribution.
4. In order to make full use of disease-resistant peas germplasm resources, the study tested the resistance of pea to powdery mildew in field natural of535pea resources from international and domestic in2009-2011.The result showed that one variety was high resistant, accounting for0.19%of the total number of appraisal; two varieties showed resistance, accounting for0.37%of the total;17varieties showed middle resistance, accounting for3.18%;60varieties showed middle susceptible, accounting for11.12%;434varieties showed susceptible, accounting for81.12%;21varieties showed high susceptible, accounting for3.93%. The resistance indoor was consistent with it in field except4middle resistance varieties through the indoor inoculation identification of20varieties which have showed resistance in field.
5. Through to research the resistance related enzyme activity change and soluble sugar, soluble protein and chlorophyll content change of different resistant varieties of pea powdery mildew before and after inoculation, the results showed that:in pea's powdery mildew resisitance response enzyme which related defense, PAL, SOD and CAT activities change had obviously positive correlation with pea's powdery mildew resistance, POD and PPO had no obvious correlation with pea's powdery mildew. After inoculation, the PAL, SOD and CAT in disease-resistant varieties had significant enzyme activity greater than susceptible varieties. In related resistance material, soluble protein and chlorophyll content had obviously positive correlation with variety resistance, soluble sugar content had negatively correlated with variety resistance. After inoculation, disease-resistant varieties of soluble protein and the chlorophyll content was significantly higher than the susceptible varieties.
引文
[1]贺晨帮,宗绪晓.豌豆种质资源形态标记遗传多样性分析[J].植物遗传资源学报,2011,12(1)：42-48
[2]陈群航,郑益嫩.福建省豌豆白粉病调查与防治[J].福建农业科技,1998,(5)：11-12
[3]周锡康,郭媛贞.大麦品种对白粉病抗病性鉴定及抗源筛选[J].植物保护学报,1997,24(1)：39-43
[4]林成辉,唐乐尘,倪伟健等.不同豌豆品种对白粉病的抗性特点与防治对策[J].中国蔬菜,2002,(6)：37-38
[5]刘海清,陈凯,徐关印.小麦不同品系白粉病抗性的鉴定[J].安徽农业科学,2006,34(6)：1151-1183
[6]郑卓杰等.中国食用豆类品种资源目录(第一集)[M].北京：中国农业科技出版社,1987
[7]郑卓杰等.中国食用豆类品种资源目录(第二集)[M].北京：中国农业科技出版社,1990
[8]龙静宜.食用豆类种植技术[M].北京：金盾出版社,2002,9
[9]胡晓,郭高球.蚕豆豌豆高产栽培[M].北京：金盾出版社,1994
[10]Branger A, Aubert G, A mau G, et al. Genetic diversity with in Pisum sativum using proteinand and PCR-based markers [J]. Theor Appl Genet,2004,108:1309-1321
[11]Burstin J, Deniot G, Potier J, et al. Microsate lite polymorphism in Pisum sativum [J]. Plant Breed,2001,120:311-317
[12]Brown A.H.D., Grace J.P, Speer S.S. Designation of a core collection of perennial Glycine soybean [J]. Genel,1987,14:59-70
[13]Fondevilla S., Avila C.M, Cubero J.I. Response to mycosphaerella pinodes in a germplasm collection of Pisum ssp.[J]. Plant breeding,2005,124(3):313-315
[14]Bashir M, Arshad M. Present status and future prospects of mash crops in Pakistan [J]. Agri.Digest,2002,1:13-20
[15]Fondevilla S., Carver T.L.W, Moreno M.T. Identification and characterization of sources of resistance to Erysiphe pisi Syd. In Pisum ssp.[J]. Plant Breeding,2007,126(2):113-119
[16]Ghafoor A., Gulbazz F.N., Afzal M, et al. Inter-relationship between SDS-PAGE markers and agronomic characters in chickper [J]. Pakistan Journal of Botany,2003,35:613-624
[17]Habib N., Zamin M. Off-season pea cultivation in Dir Kohistan valley [J]. Asian Journal of Plant Sciences,2003,2(3):283-285
[18]Harland S.C. Inheritance of immunity to powdery mildew in Peruvian forms of Pisum sativm [J]. Heredity,1948,2:263-269
[19]董玉琛.我国作物种质资源研究现状与展望[J].中国农业科技导报,1999,(2)：36-37
[20]宗绪晓,关建平,王述民,等.国外栽培豌豆遗传多样性分析及核心种质构建[J].作物学报,2008,34(9)：1518-1528
[21]Velculescu V., Zhang L., Vogelstein B, et al. Serial analysis of gene expression [J]. Science,1995,270:484-487
[22]Tzitzikas E.N., Vincken J.P., de Groot J., et al. Genetic variation in pea seed globulin composition [J] Journal of Agriculture and Food Chemistry,2006,54(2):425-433
[23]Stanton M.A., Steward J.M., Percival A.E., et al. Morphological diversity and relationship in the A-genome cottons [J]. Crop Sci.,1994,34:519-527
[24]Rakshit S., Mohapatra T., Mishra S.K. Marker assisted selection for powdery mildew resistance in pea [J]. Genet. Breed,2001,55:343-348
[25]Matsumura H., Nasir K.H.B., Yoshida K. Spuper SAGE array:the direce use of26-base-pair transcript tags in oligonucleotide arrays [J]. Nature Methods,2006,3(6):469-474
[26]柴岩,林汝法.中国小杂粮[M].北京：中国农业科学技术出版社,2002
[27]柴岩,万富世.中国小杂粮产业发展报告[M].北京：中国农业科学技术出版社,2007
[28]陈叔平主编.种质资源低温保存原理和技术[M].北京：中国农业科技出版社,1995,1-6
[29]冯钦华.青海豌豆育种栽培研究[J].青海农林科技,1999
[30]冯钦华,贺晨邦等.食荚豌豆新品种甜脆761[J].作物杂志,2000,2
[31]林凡云.中国北方春大豆遗传多样性分析[学位论文].杨凌：西北农林科技大学,2003
[32]刘澎才,吴燕.国内外种质资源研究进展[J].杂粮作物,2001,21(3)：26-27
[33]何礼.我国栽培豇豆的遗传多样性研究及其育种策略的探讨[学位论文].成都：四川大学,2002
[34]王述民,曹永生,R J Redden等.我国小豆种质资源形态多样性鉴定与分类研究[J].作物学报,2000,28(6)：727-733
[35]Xuxiao Zong, Robert J. Redden, Qingchang Liu, Shumin Wang, Jianping Guan, Jin Liu, Yanhong Xu, Peter Ades, Rebecca Ford. Analysis of a diverse global Pisum sp. Collection and comparision to a Chinese lical P. sativum collection with microsatellite markers [J]. Theor Appl Genet.,2009,118:193-204
[36]Yeh F C, Yang R C, Bovle T Popgene Version1.31Quick User Guide. University of Alberta and centre for International Forestry Research, Canada,1999
[37]Simioniuc D, Uptmoor, W. Fried, F. Ordon. Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs [J]. Plant Breeding,2002,121:429-435
[38]Samec P, Nasinec V. Detection of DNA polymorphism among pea cultivarsusing RAPD technique [J]. Biol Planta,1995,37:321-327
[39]Kumar S, Tamura K, Nei M. Integrated software for Molecular Evolutionary Genetic Analysis and sequence alignment [J]. Brief Bioinfor,2004,5:150-163
[40]Hoey B K, Crowe K R, Jone V M, Polans M O. A phylogenetic analysis of Pisum based on morphological charalters allozyme and RAPD markers [J]. Theor Appl Genet,1996,92:92-100
[41]冯夏莲,何承忠,张志毅等.植物遗传多样性研究方法概述[J].西南林学院学报,2006(1)：51-59
[42]胡志昂,张亚平.中国动植物的遗传多样性[M].南京：浙江海滨技术出版社,1996
[43]尚占环,姚爱光.生物遗传多样性研究方法及其保护措施[J].宁夏农学院学报,2002,23(1)：66-69
[44]杨志平.马铃薯种质资源的遗传多样性研究.硕士学位论文,西南大学,2006,6
[45]宋宪亮.异源四倍体棉花栽培种分子遗传图谱的构建及部分性状QTL标记定位.博士学位论文.山东农业大学,2004
[46]Bohn M. M., Khairallah D. QTL mapping in tropical maize:I.Genomic region affecting leaf feeding resistance to sugarcane borer and other traits [J]. Crop Sci,1996,36(4):1352-1361
[47]Alcazar J E. Tenth anniversary of the FAO commission on plant genetic resources:A time for taking stock and looking to challenges of the21st century [J]. Biodiversity,1993,9(3):14-16
[48]Steven.D.Tanksley. Seed Banks and Molecular Maps:Unlocking Genetic potential from the wild [J]. Science,1997,277:1063-1066
[49]Serageldin I. Genetic resources conservation in the CGIAR:Protecting an irreplaceable resource for future generation [J]. Biodiversity,1994,10(2):9-12
[50]王德利,林海俊,金晓明.吉林西部草原牧草资源的生物多样性研究[J].东北师范大学学报(自然科学版),1996(3)：103-107
[51]刘金平.西南区扁穗牛鞭草种质表型性状、遗传基础及生产性能多样性分析,博士学位论文,四川农业大学,2006,5
[52]王赞.柠条锦鸡儿遗传多样性研究.博士学位论文,中国农业大学,2005,6
[53]Bueher R E, Mcdonaid M B. Soybean cultivar identification using high performance liquid chromatography of seed proteins [J]. Crops Science,1989,29:32-37
[54]Dean, R. E., J. A. Dahlberg, M. S. Hopkins, et al. Genetic redundancy and diversity among orange accession in the U.S. national sorghum collection as assessed with simple sequence repeat (SSR) marker [J]. Crop Science,1999,39:1215-1221
[55]Dewey D.R..The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae.ln manipulation in plant improvement. Edited by J.PGustafson [J].Plenum Publishing Corp., New York.1984,207-279
[56]陈翠霞.南方玉米锈病的抗病性鉴定、抗病基因的遗传鉴定、分子标记和精细定位.博士学位论文,山东农业大学,2001
[57]杜春芳,刘惠民,李润植.单核苷酸多态性在作物遗传及改良中的应用[J].遗传,2003,25(6)：735-739
[58]车永和.冰草属植物遗传多样性取样策略基于醇溶蛋白的研究[J].植物遗传资源学报,2004,5(3)：216-221
[59]葛颂.遗传多样性及其检测方法-生物多样性原理与方法[M].北京：中国科技出版社,1994,38-43
[60]葛颂,洪德元.泡沙复合体(桔梗科)的物种生物学研究：表型的可塑性[J].植物分类学报,1994,32(6)：489-503
[61]陈传军,沈益新,周建国.南农选系草地早熟禾POD同工酶的酶谱特征及与其它品种的差异[J].中国草地,2005,27(2)：36-42
[62]程昕昕,耿广汉,刘正.玉米杂种一代的酯酶酶谱分析[J].种子,2007,26(4)：30-31
[63]邓蓉,张文,徐忠惠.紫花苜蓿三种同功酶分析[J].贵州农业科学.2005,33(6)：24-26
[64]杜红梅,李志坚,周道玮等.紫花苜蓿多叶型与三叶型品种同工酶的比较研究[J].草业学报,2005,14(1)：44-48
[65]Hamriek JL, Godt MJ. Allozyme diversity in plant species, hi:plant population genetiecs, breeding and genetic resouces [J]. Sunderland, Sinaue, Assoeiates Ine, Sunderland Publishers,1989,43-63.
[66]解新明,马万里,王振兴,等.内蒙古不同地区根茎冰草过养化物酶同工酶比较研究[J].内蒙古师大学报(自然科学汉文版),1995,(1)：51-54
[67]龚大洁,周开亚.同工酶及其在爬行动物系统学中的应用[J].西北农业大学(自然科学版),1999,35(1)：111-116
[68]胡能书,万贤国.同工酶技术及其应用[M].湖南科学技术出版社,1985
[69]李晓林.苹果属植物酯酶同工酶分析[J].西南农业大学学报,1997,19(2)：105-111
[70]刘波,王荔,陈疏影等.36份不同居群半夏同工酶研究[J].云南农业大学学报,2008,23(1)：1-18
[71]刘俊丽.新疆紫花苜蓿亮氨酸氨基肤酶、脂酶、过氧化物酶多态性及褐斑病抗性、抗寒性的研究(D).硕士学位论文,中国农业科学院,2000
[72]J.E. Gilbert, R.V.Lewis, M.J. Wilkinson, et al. Developing an appropriate strategy to assess genetic variability in plant germplasm collections.1999,98:1125-1131
[73]G.A. Moore and W. S. Castle. Morphological and Isozymic Analysis of Open-Pollinated Citrus Rootstock Populations [J].The Journal of Heredity.1988,79(1):59-63
[74]F.B.Lewu, D.S.Grierson, A.J.Afolayan, et al. Morphological diversity among accessions of Pelargonium sidoides DC. in the Eastern Cape, South Africa [J]. Genet Resour Crop Evolution (2007)54:1-6
[75]Mohammad A., Muhammad A.and Niala A. Morphological variability of Prosopis cineraria (L.) Druce from the Cholistan desert Pakistan [J]. Genetic Resources and Crop Evolution (2006)53:1589-1596
[76]Fairey N A, Coulman B E, Fairey D T, et al. Cross Canada comparison of the productivity of fodder galega (Galega orientalis L.) with traditional herbage legumes [J]. Canadian Journal of Plant Science,2000,80(4):793-800
[77]Brummer E C, Bouton J H and Kochert G.D. Development of an RFLP map in diploid alfalfa [J].Theoretical and Applied Genetics,1993,86:329-332
[78]Xu W W,Sleper D A,Chao S. Genome mapping of polyploid tall fescue (Festuca arundinacea Schreb.) with RFLP markers [J]. Theoretical and Applied Genetics,1995,91:947-955
[79]张阿英,胡宝忠,姜述君等.影响紫花苜蓿SSR分析因素的研究[J].黑龙江农业科学,2002,(1)：15-17
[80]张阿英.中国紫花苜蓿(Medicago satzva L)地方品种SSR分析[D].学位论文,东北农业大学,2002
[81]杨占花,魏臻武,雷艳芳等.两类SSR对苜蓿属种质遗传多样性和亲缘关系的比较研究[J].草地学报,2008,16(6)：559-565
[82]吴宗怀.紫花苜蓿,杂化苜蓿和黄花苜蓿SSR分子标记分析[D].硕士学位论文,内蒙古农业大学,2007
[83]魏臻武.利用SSR、ISSR和RAPD技术构建首楷基因组DNA指纹图谱[J].草业学报,2004,13(3)：62-67
[84]盛云燕,栾非时,陈克农.甜瓜SSR标记遗传多样性的研究[J].2006,37(2)：165-170
[85]解新明,卢小良.SSR和ISSR标记及其在牧草遗传与育种研究中的应用前景[J].草业科学,2005,22(2)：30-37
[86]黄建勋,张凯,江良荣等.SSR标记对籼稻品种的遗传多样性分析[J].厦门大学学报(自然科学版),2006,45(1)：120-24
[87]刘杰,刘公社等.用微卫星序列构建羊草遗传指纹图谱[J].植物学报,2000,42(9)：985-987
[88]李永祥,李立会等.披碱草属12个物种遗传多样性的ISSR和SSR比较分析[J].中国农业科学,2005,38(8)：1522-1527
[89]Bortolini Fernanda, Dall Agnol, Miguel, et al. Molecular characterization of the USDA white clover (Trifolium repens L.) core collection by RAPD markers [J]. Genetic Resources and Crop Evolution.2006,53(5):1081-1087
[90]钟小仙,白淑娟,顾洪如等.美洲狼尾草不育系、恢复系及杂种F1的RAPD分析[J].草业学报,2001,10(3)：28-32
[91]周永红,杨佼良,郑有良等.用RAPD分子标记探讨鹅观草属的种间关系[J].植物学报,1999,41(10)：1076-1081
[92]李阳春.早熟禾属植物种间关系的RAPD分析[J].草业学报,2002,11(4)：94-99
[93]Budak H, Shearman R C, Parmaksiz I, et al. Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs and SRAPs. Theoretical and Applied Genetics,2004,109:280-288
[94]韩雅莉.生物遗传多样性和系统学研究中的RAPD分析[J].内蒙古农牧学院学报,1996,20(2)：1-4
[95]李拥军,苏加楷.苜蓿地方品种遗传多样性的研究RAPD标记[J].草地学报,1998,2(6)：105-113
[96]文菊华.应用EST同工酶与RAPD对狼蛛科部分种属间的亲缘关系研究[D].硕士学位论文,湖南师范大学,2004
[97]毕玉芬,车伟光,李季蓉.利用RAPD技术研究弱秋眠性紫花苜蓿遗传多样性[J].作物学报,2005,31(5)：647-652
[98]Ravi M, Geethanjali S, Sameeyafarheen F, et al. Molecular Marker based Genetic Diversity Analysis in Rice (Oryza sativa L.) using RAPD and SSR markers [J]. Euphytica,2003,133:243-252
[99]Fuentes JL, Escobar F, Alvarez A. Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers [J]. Euphytica,1999,109:107-115
[100]Julier B, Flajoulot S, Barre P, et al. Construction of two genetic linkage maps in cultivated tetraploid alfalfa (Medieago sativa L.) using mierosatellite and AFLP markers [J]. Plant biology,2003,3(1):9
[101]Saini N, Jain N, Jain S, et al. Assessment of genetic diversity within and among basmati and non-basmati rice varieties using AFLP, ISSR and SSR markers [J]. Euphytica,2004,140:133-146
[102]Zewdu Terefework, Seppo Kaijalainen, Kristina Lindstro'm, et al. AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galega orientalis and Galega officinalis [J]. Journal of Biotechnology,2001,91:169-180
[103]Ferriol M, Pioo B, Nuez F, et al. Genetic diversity of a germplasm collection of cucurbita pepo using SRAP and AFLP markers [J]. Theoretical and Applied Genetics,2003,107:271-282
[104]彭燕,张新全,刘金平等.野生鸭茅种质遗传多样性的AFLP分子标记[J].遗传,2006,28(7)：845-850
[105]Saha M C, Mian R, Zwonitzer J C, et al. An SSR-and AFLP-based genetic linkage map of tall fescue (Festuca arundinacea Schreb.)[J]. Theoretical and Applied Genetics,2005,110:323-336
[106]白史且,高荣,沈翼等.假俭草遗传多样性的AFLP指纹分析[J].高技术通讯,2002,10,45-49
[107]刘万清.SNP-为人类基因组描绘新的蓝图[J].遗传,1998,20(6)：38-40
[108]Dorofejuk M T, Dorofejuk V F, Addrusewitsch V T, et al.Cultivation of eastern goat's rue(Galega orientalis Linn.) as a perennial fodder plant on turf podzol soils in the Republic of Belarus [J]. Archives of Agronomy and Soil Science,1999,44(2):131-148
[109]Carreck N L, Mand M, Williams I H. Goat's rue [J]. Bee World,2001,82(3):142-146
[110]Zabeau M. and Vos P. Selective reatriction fragment amplication:a general method for DNA fingerprinting [J]. Europeau Patent Application.1993
[111]Mori A, Cohen B D, Lowenthal A, et al. Guanidines-historical, biological, biochemical and clinical aspects of thenaturally occurring guanidine compounds [M]. London:Plenum Press,1985
[112]Fernando GA, Pedro A.R, Raquel P, et al. Management of Galega officinalis L. and preliminary results on its potential for milk production improvement in sheep [J]. New Zealand Journal of Agricultural Research,2004,47:233-245
[113]郭大龙,罗正荣.部分柿属植物SRAP-PCR反应体系的优化[J].果树学报.2006,23(1)：138-141
[114]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica [J]. Theoretical and Applied Genetics,2001,103:455-461
[115]郭素英.黄瓜F基因的SRAP分子标记.硕士学位论文,西南农业大学,2005
[116]Budak H, Shearman R C, Parmaksiz I, et al. Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs and SRAPs [J]. Theoretical and Applied Genetics,2004,109:280-288
[117]易杨杰.野生狗牙根种质遗传多样性的SRAP研究[J].遗传,2008,30(1)：94-100
[118]Charters Y M, Robertson A, Wilkinson M J. et al. PCR analysis of oil seed rape cultivars (Brassica napus L,SSP.Oleifera) using5-anchored Simple sequence repeats primer. Theor.Appl.Genet,1996,92:442-447
[119]王心宇,陈佩度,元增军等ISSR标记在小麦指纹图谱分析中的应用研究初探[J].农业生物技术学报,2001,9(3)：261-263
[120]宣继萍,章缜,房经贵等.苹果品种指纹图谱构建.果树学报,2002,19(6)：421-423
[121]Tanksley SD, Medina-Filho H, Rick CM. Use of naturally-occurring enzyme variation to detect and map gene controlling quantitative traits in an interspecific backcross of tomato. Heredity,1982,49:11-45
[122]Nagaoka A, Olihara Y. Applicability of Inter-simple sequence repeats polymorphisms in wheat for use DNA markers in comparison to RFLP and RAPD markers. Theor. Appl. Genet.1997,597-602
[123]江树业,陈启锋,方宣钧.水稻农垦58与农垦58S的RAPD和ISSR分析[J].农业生物技术学报,2000,1(1)：63-66
[124]Fang D Q, Roose ML, Identification of closely related with ISSR markers [J]. Theor. Appl. Genet.1997,95:408-417
[125]Prevost A, Wilkinson M. J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor. Appl. Genet,1999,98:107-112
[126]赵国芳ISSR对梨属栽培品种(Pyrus L.)基因组的指纹分析.河北农业大学硕士论文,2003,5
[127]J.C. Huang, M.Sun. Genetic diversity and relationships of sweet potato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis o chloroplast DNA [J]. Theor Appl Genet,2000,100:1050-1060
[128]Tkojima, T. Nagaoka, K. Nooda. Genetic linkage map of ISSR and RAPD Markers in Einkorn wheat in relation to that of RFLP markers [J]. Theor Appl Genet,1998,96:37-45
[129]席嘉宾,郑玉忠,杨中艺.地毯草ISSR反应体系的建立与优化[J].中山大学学报(自然科学版),2004,25(3)：80-84
[130]王瑜,袁庆华.紫花苜蓿ISSR-PCR反应体系的建立与优化[J].草地学报,2007,15(3)：212-215
[131]尚海英,郑有良,魏育明等.应用ISSR标记研究黑麦草属植物遗传多样性[J].西南农业学报,2004,17(3)：273-277
[132]祁建民,王涛,陈顺辉,周东新等.部分烟草种质遗传多样性与亲缘关系的ISSR标记分析[J].作物学报,2006,32(2)：373-378
[133]穆立蔷,刘赢男,冯富娟等.紫椴ISSR-PCR反应体系的建立与优化[J].林业科学,2006,42(6)：26-31
[134]汪为民.豌豆数量性状的基因效应分析[J].上海农学院学报,1997,15(2)：104-108
[135]杨俊品.豌豆主要农艺性状的配合力分析[J].四川农业大学学报,1996,14(3)：378-381
[136]纪玉贵.食荚豌豆雄性不育突变体的遗传研究[J].华北农学报,2005,20(1)：63-66
[137]王凤宝.豌豆半无叶突变体性状的遗传及在育种上的利用[J].遗传,2003,25(2)：185-188
[138]王凤宝.半无叶型菜豌豆af基因遗传研究及其利用[J].园艺学报,2004,31(6)：747-750
[139]赵秀榆.宁南霉素防治菜豌豆白粉病[J].农药,2000,29(4)：41
[140]陈振落.甜豌豆白粉病的发生与防治[J].福建农业,2004,(2)：20-21
[141]刘爱媛.豌豆离体叶片鉴定白粉病抗性方法[J].植物保护学报,2002,29(2)：119-123
[142]黄旭正.冬种豌豆白粉病及防治方法[J].蔬菜生产,2002,29(2)：12
[143]Davidson J A, Krysinska-Kaczmarek M, Kimber RBE, Ramsey MD. Screening field pea germplasm for resistance to downy mildew (Peronospora viciae) and powdery milderw (Erysiphe pisi)[J]. Australasian Plant Pathology.2004,33(3):413-417
[144]Diego R, Monica FA, Eleonora B, Josefina CS and Sara F. Disease Resistance in pea (Pisum sativum L.) Types for Autumn Sowings in Mediterranean Environments [J]. Plant Breed.2009,45(4):135-142
[145]Dirlewanger E, Isaacp G, Ranade S. Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and development traits in Pisum sativum L.[J]. Appl. Genet.1994,88:17-27
[146]Eklund M, Tuvesson S, Microsat. Ellite markers for powdery mildew resistance in pea Pisum sativum L.[J]. Hereditas.2005,142:86-91
[147]Fondevilla S, Carver TLW, Moreno MT, Rubiales D. Macroscopic and histological characterization of genes erl and er2for powdery mildew resistance in pea [J]. Eur J Plant Pathol.2006,115:309-321
[148]彭化贤,姚革.我国豌豆地方品种抗白粉病性的研究[J].植物病理学报,1990,23：62
[149]仇元.牧草及绿肥作物病害[M].北京：高等教育出版社,1959,419
[150]Wendy C Morgan, Parbery D G. Effects of Pseudopeziza leaf spot disease on growth and yield in Lucerne [J]. Aust J Agric Res,1977,(1):1029-1040
[151]南志标,李春杰.中国牧草真菌病害名录[J].草业科学,1994,(增刊)：611
[152]张蓉,马建华.宁夏苜蓿病虫害发生现状及防治对策[J].草业科学,2003,20(6)：40-44
[153]N.E.希斯,R.F巴恩斯,D.S.梅特卡夫主编.黄文惠,苏加楷,张玉发,等译.牧草-草地农业科学[M].北京：农业出版社,1992.68-86
[154]韩金声.牧草病害[M].北京：北京农业大学出版社,1988,163-165
[155]杨德良,刘有德.43%好力克悬浮剂防治大荚豌豆白粉病锈病田间试验[J].植物保护,2002,3：27-29
[156]王兆美,字迎彪,杨锡银等.30%特富灵粉剂防治大荚豌豆白粉病试验[J].蔬菜,2000,(6)：27
[157]Pammel, L.H. Fungus disease of orange plants [J].Mo Rev.Iowa Weather Crop Serv,1891,2,8-9
[158]Le Clerg, E.C. Crop losses due to plant diseasesin the United States [J]. Phytopathology,1964,54:1309-1313
[159]Anon. Losses in agriculture [M]. U.S. Dep. Agric.Handb.1965,291
[160]肖悦岩,季伯衡,杨之为.植物病害流行与预测[M].北京：中国农业大学出版社,1998,124-137
[161]曾士迈.植保系统工程导论[M].北京：北京农业大学出版社,1994
[162]曾士迈,杨演.植物病害流行学[M].北京：农业出版社,1986,87-117
[163]Willis, C.B. Observations on the disease of forage crops in Prince Edward Island [J]. Can. Plant Dis. Surv.1965,45,8-11
[164]Horsfall, J.G. A study of meadow crop disease in New York [J]. Cornell Univ. Agric. Exp. Stn. Men.,1930,130
[165]曾士迈.著名专家、学者对21世纪我国植物病虫害可持续控制的展望[J].植物保护,1998(6)：35-37
[166]李志勇.牧草种质资源描述规范和数据标准[M].北京：中国农业出版社,2005
[167]明军,顾万春.紫丁香表型多样性研究[J].林业科学研究,2006,19(2)：199-204
[168]竺利波,顾万春,李斌.紫荆群体表型性状多样性研究[J].林业科学,2007,23(3)：138-145
[169]王俊娥,王赞,王运琦,等.山羊豆种质资源形态多样性分析[J].植物遗传资源学报,2008,9(2)：201-205
[170]王志刚.菜用豌豆种质资源形态性状遗传多样性分析.硕士学位论文,中国农业科学院,2009
[171]Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification [J]. Genomics,1994,20:176-183
[172]王瑜,袁庆华.紫花苜蓿ISSR-PCR反应体系的建立与优化[J].草地学报,2007,15(3)：212-215
[173]Godwin I D, Aitken E A, Smith L W. Application of intersimple sequence repeat (ISSR) markers to plant genetics [J]. Electrophoresis,1997,18:1524-1528
[174]顾红雅,瞿礼嘉.植物分子生物学实验手册[M].北京：高等教育出版社,1998
[175]王关林,方宏筠.植物基因工程原理与技术[M].北京：科学出版社,1998
[176]王方,袁庆华.冰草ISSR-PCR反应体系的建立与优化[J].草地学报,2009,17(3)：354-357
[177]袁庆华,张君艳.苜蓿假盘菌ISSR反应体系优化及指纹图谱构建[J].草地学报,2008,16(1)：17-22
[178]卢萍,陈曦,赵萌莉.砂珍棘豆ISSR-PCR反应条件优化研究[J].华北农学报,2008,23(5)：104-106
[179]胡甦,王永清,陶炼,等.三角紫叶酢浆草ISSR反应体系的建立与优化[J].草业学报,2011,20(5)：142-150
[180]冯亮亮,唐红,李毅,等.甘肃红砂不同种群遗传多样性的ISSR分析[J].草业学报,2011,20(1)；125-130
[181]李鸿雁,李志勇,王小丽,等.内蒙古扁蓿豆遗传多样性的ISSR分析[J].西北植物学报,2011,31(1)：0052-0056
[182]王照兰,杨持,赵丽丽,等.扁蓿豆不同品系ISSR标记遗传差异和遗传多样性[J].中国草地学报,2010,32(1)：11-17
[183]耿立格,王丽娜,张磊,等.河北省绿子叶黑豆种质资源表现型和ISSR标记遗传多样性分析[J].植物遗传资源学报,2010,11(3)：266-270
[184]卢萍,赵萌莉,韩国栋,等.内蒙古小花棘豆遗传多样性的ISSR分析[J].西北植物学报,2007,27(6)：1102-1107
[185]宗绪晓,Rebecca Ford, Robert R Redden,等.豌豆属SSR标记遗传多样性结构鉴别与分析[J].中国农业科学,2009,42(1)：36-46
[186]陈永霞,张新全,谢文刚,等.利用EST-SSR标记分析西南扁穗牛鞭草种质的遗传多样性[J].草业学报,2011,20(6)：245-253
[187]王海飞,关建平,马钰,等.中国蚕豆种质资源ISSR标记遗传多样性分析[J].作物学报,2011,37(4)：595-602
[188]刘忠辉,刘国道,黄必志,等.云南猪屎豆属遗传多样性的ISSR分析[J].草业学报,2009,18(5)：184-191
[189]范彦,李芳,张新全,等.扁穗牛鞭草种质遗传多样性的ISSR分析[J].草业学报,2007,16(4)：76-81
[190]周泽扬,夏庆友,鲁成,等.分子系统生物学研究中分子位点数与遗传差异信息可靠性的关系[Jl遗传,1998,20(5)：12-15
[191]陈群航,郑益嫩.福建省豌豆白粉病调查与防治[J].福建农业科技,1998,(5)：11-12
[192]王生荣,陈诚,柴兆祥,等.甘肃省草本植物白粉菌调查(一)[Jl.草原与草坪,2001,(1)：25-27
[193]胡锐,邢彩云,吴营昌,等.11个优质小麦品种对小麦白粉病抗性的初步鉴定[J].河南农业科学,2011,40(5)：108-110
[194]Braun U. The Powdery Mildews (Erysiphales) of Europe. Stuttgart:Gustav Fischer Verlag [M]1995
[195]盖钧镒.试验统计方法[M].北京：中国农业出版社,2000
[196]林成辉,唐乐尘,倪伟健,等.不同豌豆品种对白粉病的抗性特点与防治对策[J].中国蔬菜,2002,(6)：37-38
[197]彭化贤,姚革,贾瑞林,等.豌豆抗白粉病资源鉴定研究[J].西南农业大学学报,1991,13(4)：384-386
[198]Amrish Vaid, P.D. Tyagi豌豆白粉病抗性的遗传学[J].国外农学—杂粮作物,1998,18(5)：14-16
[199]王昶,杨晓明,杨发荣等.陇豌1号在西北豌豆种植区最适密度研究[J].中国种业,2011,(2)：47-48
[200]刘微,刘淑艳,李玉,等.番茄白粉病的病原菌鉴定[J].植物病理学报,2009,39(1)：11-15
[201]邵伯飞,胡东维,李德葆.大豆与白粉病菌相互作用的超微结构与细胞化学[J].电子显微学报,2001,20(6)：744-747
[202]刘东顺,程鸿,孔维萍,等.甘肃甜瓜主产区白粉病菌生理小种的鉴定[J].中国蔬菜,2010,(6)：28-32
[203]王娟,宫国义,郭绍贵,等.北京地区瓜类蔬菜白粉病菌生理小种分化的初步鉴定[J].中国蔬菜,2006,(8)：7-9
[204]邢会琴,李敏权,徐秉良,等.气孔与苜蓿品种对白粉病抗性的关系[J].草原与草坪,2003,(3)：42-45
[205]刘晓玲,宋超,杜文华.红三叶对白粉病抗性机理研究[J].草原与草坪,2011,31(3)：73-76
[206]Ahl-Coy P, Felix G, Metraux JP, et al. Resistance to disease in the hybrid Nicotiana glutinosa x Nicotiana debneyi is associated with high constitutive levels β-1,3-glucanase, chitinas, peroxidase and polyphenoloxidase. Physiol. Mol. Pathol.1992,41:11-21
[207]李靖,利容千,袁文静.黄瓜感染霜霉病菌叶片中一些酶活性的变化[J].植物病理学报,1991,21(4)：277-283
[208]董毅敏,徐建华,郭季芳.抗病和感病黄瓜品种感染白粉菌后几种酶活性的变化[J].植物学报,1990,32(2)：160-164
[209]李明岩,屈淑平,崔崇士.利用酶活性鉴定南瓜对白粉病菌抗病性的研究[J].东北农业大学学报,2007,38(6)：737-741
[210]李惠霞,王蒂.马铃薯晚疫病抗性反应中木质素及防御酶活性的变化[J].甘肃农业大学学报,2006,41(3)：52-56
[211]周晓慧,Wolukau JN,李英,等.甜瓜蔓枯病抗性与SOD、CAT、和POD活性变化的关系[J].中国瓜菜,2007,(2)：4-6
[212]云兴福,崔世茂,霍秀文.黄瓜组织中几种酶活性与其对霜霉病抗性的关系lJl.华北农学报,1995,10(1)：92-98
[213]孔凡明,许志刚.水稻不育系抗白枯病与体内酶活性变化的关系lJl.安徽农业大学学报,1998,25(3)：217-223
[214]邹琦.植物生理学实验指导[M].北京：中国农业出版社,2000,163-164
[215]王保成,孙万仓,范惠玲,等.芸芥自交亲和系与自交不亲和系SOD、POD和CAT酶活性lJl.中国油料作物学报,2006,28(2)：162-165
[216]张妙霞,孔祥生.蒽酮法测定可溶性糖显色条件的研究[J].洛阳农专学报,1997,17(4)：24-28
[217]邹琦.植物生理学实验指导[M].北京：中国农业出版社,2000,111-112
[218]邹琦.植物生理学实验指导[M].北京：中国农业出版社,2000,129-130
[219]邹琦.植物生理学实验指导[M].北京：中国农业出版社,2000,72-75
[220]毛晓英,张玉岭,李茂盛,等.BTH对新疆甜瓜抗病性的诱导研究[J].新疆农业科学,2005,42(3)：158-161
[221]Graham MY, Graham TL. Rapid accumulation of anionic peroxidases and phenolic polymers in soybean cotyledon tissues following treatment with Phytophthora megasperma F. sp. Glycinea wall glucan[J]. Plant Physiol.1991,97:1445-1455
[222]王国梁,卢浩然,陈启峰.水稻品种抗瘟性生化鉴定的研究[J].福建农学院学报,1986,15(3)：195-203
[223]董汉松.植物诱导抗病性原理与研究[M].北京：科学出版社,1995,160-170
[224]李合生,李琳,焦新之.现代植物生理学实验指南[M].北京：科学出版社,1999,164-165
[225]潘瑞炽.植物生理学(第四版)[M].北京：高等教育出版社,2001,112
[226]贺立红,宾金华.高等植物中的多酚氧化酶[J].植物生理学通讯,2001,37(4)：340-345
[227]张欣.与植物抗病性有关酶的研究进展[J].华南热带农业大学学报,2000,6(1)：41-46
[228]云兴福.黄瓜组织中氨基酸、糖和叶绿素含量与其对霜霉病抗性的关系[J].华南农学报,1993,8(4)：52-58
[229]刘素萍,王汝贤.根系分泌物中糖和氨基酸对棉花枯萎菌的影响[J].西北农业大学学报,1998,26(6)：30-31
[230]龙书生,李亚玲.糖分含量作为抗镰刀菌茎腐病玉米品种的育种指标研究[J].山东农业大学学报,1999,30(4)：372-380
[231]骆桂芬,崔俊涛.黄瓜叶片中糖和木质素含量与霜霉病诱导抗性的关系[J].植物病理学报,1997,27(1)：65-69
[2]陈群航,郑益嫩.福建省豌豆白粉病调查与防治[J].福建农业科技,1998,(5)：11-12
[3]周锡康,郭媛贞.大麦品种对白粉病抗病性鉴定及抗源筛选[J].植物保护学报,1997,24(1)：39-43
[4]林成辉,唐乐尘,倪伟健等.不同豌豆品种对白粉病的抗性特点与防治对策[J].中国蔬菜,2002,(6)：37-38
[5]刘海清,陈凯,徐关印.小麦不同品系白粉病抗性的鉴定[J].安徽农业科学,2006,34(6)：1151-1183
[6]郑卓杰等.中国食用豆类品种资源目录(第一集)[M].北京：中国农业科技出版社,1987
[7]郑卓杰等.中国食用豆类品种资源目录(第二集)[M].北京：中国农业科技出版社,1990
[8]龙静宜.食用豆类种植技术[M].北京：金盾出版社,2002,9
[9]胡晓,郭高球.蚕豆豌豆高产栽培[M].北京：金盾出版社,1994
[10]Branger A, Aubert G, A mau G, et al. Genetic diversity with in Pisum sativum using proteinand and PCR-based markers [J]. Theor Appl Genet,2004,108:1309-1321
[11]Burstin J, Deniot G, Potier J, et al. Microsate lite polymorphism in Pisum sativum [J]. Plant Breed,2001,120:311-317
[12]Brown A.H.D., Grace J.P, Speer S.S. Designation of a core collection of perennial Glycine soybean [J]. Genel,1987,14:59-70
[13]Fondevilla S., Avila C.M, Cubero J.I. Response to mycosphaerella pinodes in a germplasm collection of Pisum ssp.[J]. Plant breeding,2005,124(3):313-315
[14]Bashir M, Arshad M. Present status and future prospects of mash crops in Pakistan [J]. Agri.Digest,2002,1:13-20
[15]Fondevilla S., Carver T.L.W, Moreno M.T. Identification and characterization of sources of resistance to Erysiphe pisi Syd. In Pisum ssp.[J]. Plant Breeding,2007,126(2):113-119
[16]Ghafoor A., Gulbazz F.N., Afzal M, et al. Inter-relationship between SDS-PAGE markers and agronomic characters in chickper [J]. Pakistan Journal of Botany,2003,35:613-624
[17]Habib N., Zamin M. Off-season pea cultivation in Dir Kohistan valley [J]. Asian Journal of Plant Sciences,2003,2(3):283-285
[18]Harland S.C. Inheritance of immunity to powdery mildew in Peruvian forms of Pisum sativm [J]. Heredity,1948,2:263-269
[19]董玉琛.我国作物种质资源研究现状与展望[J].中国农业科技导报,1999,(2)：36-37
[20]宗绪晓,关建平,王述民,等.国外栽培豌豆遗传多样性分析及核心种质构建[J].作物学报,2008,34(9)：1518-1528
[21]Velculescu V., Zhang L., Vogelstein B, et al. Serial analysis of gene expression [J]. Science,1995,270:484-487
[22]Tzitzikas E.N., Vincken J.P., de Groot J., et al. Genetic variation in pea seed globulin composition [J] Journal of Agriculture and Food Chemistry,2006,54(2):425-433
[23]Stanton M.A., Steward J.M., Percival A.E., et al. Morphological diversity and relationship in the A-genome cottons [J]. Crop Sci.,1994,34:519-527
[24]Rakshit S., Mohapatra T., Mishra S.K. Marker assisted selection for powdery mildew resistance in pea [J]. Genet. Breed,2001,55:343-348
[25]Matsumura H., Nasir K.H.B., Yoshida K. Spuper SAGE array:the direce use of26-base-pair transcript tags in oligonucleotide arrays [J]. Nature Methods,2006,3(6):469-474
[26]柴岩,林汝法.中国小杂粮[M].北京：中国农业科学技术出版社,2002
[27]柴岩,万富世.中国小杂粮产业发展报告[M].北京：中国农业科学技术出版社,2007
[28]陈叔平主编.种质资源低温保存原理和技术[M].北京：中国农业科技出版社,1995,1-6
[29]冯钦华.青海豌豆育种栽培研究[J].青海农林科技,1999
[30]冯钦华,贺晨邦等.食荚豌豆新品种甜脆761[J].作物杂志,2000,2
[31]林凡云.中国北方春大豆遗传多样性分析[学位论文].杨凌：西北农林科技大学,2003
[32]刘澎才,吴燕.国内外种质资源研究进展[J].杂粮作物,2001,21(3)：26-27
[33]何礼.我国栽培豇豆的遗传多样性研究及其育种策略的探讨[学位论文].成都：四川大学,2002
[34]王述民,曹永生,R J Redden等.我国小豆种质资源形态多样性鉴定与分类研究[J].作物学报,2000,28(6)：727-733
[35]Xuxiao Zong, Robert J. Redden, Qingchang Liu, Shumin Wang, Jianping Guan, Jin Liu, Yanhong Xu, Peter Ades, Rebecca Ford. Analysis of a diverse global Pisum sp. Collection and comparision to a Chinese lical P. sativum collection with microsatellite markers [J]. Theor Appl Genet.,2009,118:193-204
[36]Yeh F C, Yang R C, Bovle T Popgene Version1.31Quick User Guide. University of Alberta and centre for International Forestry Research, Canada,1999
[37]Simioniuc D, Uptmoor, W. Fried, F. Ordon. Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs [J]. Plant Breeding,2002,121:429-435
[38]Samec P, Nasinec V. Detection of DNA polymorphism among pea cultivarsusing RAPD technique [J]. Biol Planta,1995,37:321-327
[39]Kumar S, Tamura K, Nei M. Integrated software for Molecular Evolutionary Genetic Analysis and sequence alignment [J]. Brief Bioinfor,2004,5:150-163
[40]Hoey B K, Crowe K R, Jone V M, Polans M O. A phylogenetic analysis of Pisum based on morphological charalters allozyme and RAPD markers [J]. Theor Appl Genet,1996,92:92-100
[41]冯夏莲,何承忠,张志毅等.植物遗传多样性研究方法概述[J].西南林学院学报,2006(1)：51-59
[42]胡志昂,张亚平.中国动植物的遗传多样性[M].南京：浙江海滨技术出版社,1996
[43]尚占环,姚爱光.生物遗传多样性研究方法及其保护措施[J].宁夏农学院学报,2002,23(1)：66-69
[44]杨志平.马铃薯种质资源的遗传多样性研究.硕士学位论文,西南大学,2006,6
[45]宋宪亮.异源四倍体棉花栽培种分子遗传图谱的构建及部分性状QTL标记定位.博士学位论文.山东农业大学,2004
[46]Bohn M. M., Khairallah D. QTL mapping in tropical maize:I.Genomic region affecting leaf feeding resistance to sugarcane borer and other traits [J]. Crop Sci,1996,36(4):1352-1361
[47]Alcazar J E. Tenth anniversary of the FAO commission on plant genetic resources:A time for taking stock and looking to challenges of the21st century [J]. Biodiversity,1993,9(3):14-16
[48]Steven.D.Tanksley. Seed Banks and Molecular Maps:Unlocking Genetic potential from the wild [J]. Science,1997,277:1063-1066
[49]Serageldin I. Genetic resources conservation in the CGIAR:Protecting an irreplaceable resource for future generation [J]. Biodiversity,1994,10(2):9-12
[50]王德利,林海俊,金晓明.吉林西部草原牧草资源的生物多样性研究[J].东北师范大学学报(自然科学版),1996(3)：103-107
[51]刘金平.西南区扁穗牛鞭草种质表型性状、遗传基础及生产性能多样性分析,博士学位论文,四川农业大学,2006,5
[52]王赞.柠条锦鸡儿遗传多样性研究.博士学位论文,中国农业大学,2005,6
[53]Bueher R E, Mcdonaid M B. Soybean cultivar identification using high performance liquid chromatography of seed proteins [J]. Crops Science,1989,29:32-37
[54]Dean, R. E., J. A. Dahlberg, M. S. Hopkins, et al. Genetic redundancy and diversity among orange accession in the U.S. national sorghum collection as assessed with simple sequence repeat (SSR) marker [J]. Crop Science,1999,39:1215-1221
[55]Dewey D.R..The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae.ln manipulation in plant improvement. Edited by J.PGustafson [J].Plenum Publishing Corp., New York.1984,207-279
[56]陈翠霞.南方玉米锈病的抗病性鉴定、抗病基因的遗传鉴定、分子标记和精细定位.博士学位论文,山东农业大学,2001
[57]杜春芳,刘惠民,李润植.单核苷酸多态性在作物遗传及改良中的应用[J].遗传,2003,25(6)：735-739
[58]车永和.冰草属植物遗传多样性取样策略基于醇溶蛋白的研究[J].植物遗传资源学报,2004,5(3)：216-221
[59]葛颂.遗传多样性及其检测方法-生物多样性原理与方法[M].北京：中国科技出版社,1994,38-43
[60]葛颂,洪德元.泡沙复合体(桔梗科)的物种生物学研究：表型的可塑性[J].植物分类学报,1994,32(6)：489-503
[61]陈传军,沈益新,周建国.南农选系草地早熟禾POD同工酶的酶谱特征及与其它品种的差异[J].中国草地,2005,27(2)：36-42
[62]程昕昕,耿广汉,刘正.玉米杂种一代的酯酶酶谱分析[J].种子,2007,26(4)：30-31
[63]邓蓉,张文,徐忠惠.紫花苜蓿三种同功酶分析[J].贵州农业科学.2005,33(6)：24-26
[64]杜红梅,李志坚,周道玮等.紫花苜蓿多叶型与三叶型品种同工酶的比较研究[J].草业学报,2005,14(1)：44-48
[65]Hamriek JL, Godt MJ. Allozyme diversity in plant species, hi:plant population genetiecs, breeding and genetic resouces [J]. Sunderland, Sinaue, Assoeiates Ine, Sunderland Publishers,1989,43-63.
[66]解新明,马万里,王振兴,等.内蒙古不同地区根茎冰草过养化物酶同工酶比较研究[J].内蒙古师大学报(自然科学汉文版),1995,(1)：51-54
[67]龚大洁,周开亚.同工酶及其在爬行动物系统学中的应用[J].西北农业大学(自然科学版),1999,35(1)：111-116
[68]胡能书,万贤国.同工酶技术及其应用[M].湖南科学技术出版社,1985
[69]李晓林.苹果属植物酯酶同工酶分析[J].西南农业大学学报,1997,19(2)：105-111
[70]刘波,王荔,陈疏影等.36份不同居群半夏同工酶研究[J].云南农业大学学报,2008,23(1)：1-18
[71]刘俊丽.新疆紫花苜蓿亮氨酸氨基肤酶、脂酶、过氧化物酶多态性及褐斑病抗性、抗寒性的研究(D).硕士学位论文,中国农业科学院,2000
[72]J.E. Gilbert, R.V.Lewis, M.J. Wilkinson, et al. Developing an appropriate strategy to assess genetic variability in plant germplasm collections.1999,98:1125-1131
[73]G.A. Moore and W. S. Castle. Morphological and Isozymic Analysis of Open-Pollinated Citrus Rootstock Populations [J].The Journal of Heredity.1988,79(1):59-63
[74]F.B.Lewu, D.S.Grierson, A.J.Afolayan, et al. Morphological diversity among accessions of Pelargonium sidoides DC. in the Eastern Cape, South Africa [J]. Genet Resour Crop Evolution (2007)54:1-6
[75]Mohammad A., Muhammad A.and Niala A. Morphological variability of Prosopis cineraria (L.) Druce from the Cholistan desert Pakistan [J]. Genetic Resources and Crop Evolution (2006)53:1589-1596
[76]Fairey N A, Coulman B E, Fairey D T, et al. Cross Canada comparison of the productivity of fodder galega (Galega orientalis L.) with traditional herbage legumes [J]. Canadian Journal of Plant Science,2000,80(4):793-800
[77]Brummer E C, Bouton J H and Kochert G.D. Development of an RFLP map in diploid alfalfa [J].Theoretical and Applied Genetics,1993,86:329-332
[78]Xu W W,Sleper D A,Chao S. Genome mapping of polyploid tall fescue (Festuca arundinacea Schreb.) with RFLP markers [J]. Theoretical and Applied Genetics,1995,91:947-955
[79]张阿英,胡宝忠,姜述君等.影响紫花苜蓿SSR分析因素的研究[J].黑龙江农业科学,2002,(1)：15-17
[80]张阿英.中国紫花苜蓿(Medicago satzva L)地方品种SSR分析[D].学位论文,东北农业大学,2002
[81]杨占花,魏臻武,雷艳芳等.两类SSR对苜蓿属种质遗传多样性和亲缘关系的比较研究[J].草地学报,2008,16(6)：559-565
[82]吴宗怀.紫花苜蓿,杂化苜蓿和黄花苜蓿SSR分子标记分析[D].硕士学位论文,内蒙古农业大学,2007
[83]魏臻武.利用SSR、ISSR和RAPD技术构建首楷基因组DNA指纹图谱[J].草业学报,2004,13(3)：62-67
[84]盛云燕,栾非时,陈克农.甜瓜SSR标记遗传多样性的研究[J].2006,37(2)：165-170
[85]解新明,卢小良.SSR和ISSR标记及其在牧草遗传与育种研究中的应用前景[J].草业科学,2005,22(2)：30-37
[86]黄建勋,张凯,江良荣等.SSR标记对籼稻品种的遗传多样性分析[J].厦门大学学报(自然科学版),2006,45(1)：120-24
[87]刘杰,刘公社等.用微卫星序列构建羊草遗传指纹图谱[J].植物学报,2000,42(9)：985-987
[88]李永祥,李立会等.披碱草属12个物种遗传多样性的ISSR和SSR比较分析[J].中国农业科学,2005,38(8)：1522-1527
[89]Bortolini Fernanda, Dall Agnol, Miguel, et al. Molecular characterization of the USDA white clover (Trifolium repens L.) core collection by RAPD markers [J]. Genetic Resources and Crop Evolution.2006,53(5):1081-1087
[90]钟小仙,白淑娟,顾洪如等.美洲狼尾草不育系、恢复系及杂种F1的RAPD分析[J].草业学报,2001,10(3)：28-32
[91]周永红,杨佼良,郑有良等.用RAPD分子标记探讨鹅观草属的种间关系[J].植物学报,1999,41(10)：1076-1081
[92]李阳春.早熟禾属植物种间关系的RAPD分析[J].草业学报,2002,11(4)：94-99
[93]Budak H, Shearman R C, Parmaksiz I, et al. Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs and SRAPs. Theoretical and Applied Genetics,2004,109:280-288
[94]韩雅莉.生物遗传多样性和系统学研究中的RAPD分析[J].内蒙古农牧学院学报,1996,20(2)：1-4
[95]李拥军,苏加楷.苜蓿地方品种遗传多样性的研究RAPD标记[J].草地学报,1998,2(6)：105-113
[96]文菊华.应用EST同工酶与RAPD对狼蛛科部分种属间的亲缘关系研究[D].硕士学位论文,湖南师范大学,2004
[97]毕玉芬,车伟光,李季蓉.利用RAPD技术研究弱秋眠性紫花苜蓿遗传多样性[J].作物学报,2005,31(5)：647-652
[98]Ravi M, Geethanjali S, Sameeyafarheen F, et al. Molecular Marker based Genetic Diversity Analysis in Rice (Oryza sativa L.) using RAPD and SSR markers [J]. Euphytica,2003,133:243-252
[99]Fuentes JL, Escobar F, Alvarez A. Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers [J]. Euphytica,1999,109:107-115
[100]Julier B, Flajoulot S, Barre P, et al. Construction of two genetic linkage maps in cultivated tetraploid alfalfa (Medieago sativa L.) using mierosatellite and AFLP markers [J]. Plant biology,2003,3(1):9
[101]Saini N, Jain N, Jain S, et al. Assessment of genetic diversity within and among basmati and non-basmati rice varieties using AFLP, ISSR and SSR markers [J]. Euphytica,2004,140:133-146
[102]Zewdu Terefework, Seppo Kaijalainen, Kristina Lindstro'm, et al. AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galega orientalis and Galega officinalis [J]. Journal of Biotechnology,2001,91:169-180
[103]Ferriol M, Pioo B, Nuez F, et al. Genetic diversity of a germplasm collection of cucurbita pepo using SRAP and AFLP markers [J]. Theoretical and Applied Genetics,2003,107:271-282
[104]彭燕,张新全,刘金平等.野生鸭茅种质遗传多样性的AFLP分子标记[J].遗传,2006,28(7)：845-850
[105]Saha M C, Mian R, Zwonitzer J C, et al. An SSR-and AFLP-based genetic linkage map of tall fescue (Festuca arundinacea Schreb.)[J]. Theoretical and Applied Genetics,2005,110:323-336
[106]白史且,高荣,沈翼等.假俭草遗传多样性的AFLP指纹分析[J].高技术通讯,2002,10,45-49
[107]刘万清.SNP-为人类基因组描绘新的蓝图[J].遗传,1998,20(6)：38-40
[108]Dorofejuk M T, Dorofejuk V F, Addrusewitsch V T, et al.Cultivation of eastern goat's rue(Galega orientalis Linn.) as a perennial fodder plant on turf podzol soils in the Republic of Belarus [J]. Archives of Agronomy and Soil Science,1999,44(2):131-148
[109]Carreck N L, Mand M, Williams I H. Goat's rue [J]. Bee World,2001,82(3):142-146
[110]Zabeau M. and Vos P. Selective reatriction fragment amplication:a general method for DNA fingerprinting [J]. Europeau Patent Application.1993
[111]Mori A, Cohen B D, Lowenthal A, et al. Guanidines-historical, biological, biochemical and clinical aspects of thenaturally occurring guanidine compounds [M]. London:Plenum Press,1985
[112]Fernando GA, Pedro A.R, Raquel P, et al. Management of Galega officinalis L. and preliminary results on its potential for milk production improvement in sheep [J]. New Zealand Journal of Agricultural Research,2004,47:233-245
[113]郭大龙,罗正荣.部分柿属植物SRAP-PCR反应体系的优化[J].果树学报.2006,23(1)：138-141
[114]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica [J]. Theoretical and Applied Genetics,2001,103:455-461
[115]郭素英.黄瓜F基因的SRAP分子标记.硕士学位论文,西南农业大学,2005
[116]Budak H, Shearman R C, Parmaksiz I, et al. Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs and SRAPs [J]. Theoretical and Applied Genetics,2004,109:280-288
[117]易杨杰.野生狗牙根种质遗传多样性的SRAP研究[J].遗传,2008,30(1)：94-100
[118]Charters Y M, Robertson A, Wilkinson M J. et al. PCR analysis of oil seed rape cultivars (Brassica napus L,SSP.Oleifera) using5-anchored Simple sequence repeats primer. Theor.Appl.Genet,1996,92:442-447
[119]王心宇,陈佩度,元增军等ISSR标记在小麦指纹图谱分析中的应用研究初探[J].农业生物技术学报,2001,9(3)：261-263
[120]宣继萍,章缜,房经贵等.苹果品种指纹图谱构建.果树学报,2002,19(6)：421-423
[121]Tanksley SD, Medina-Filho H, Rick CM. Use of naturally-occurring enzyme variation to detect and map gene controlling quantitative traits in an interspecific backcross of tomato. Heredity,1982,49:11-45
[122]Nagaoka A, Olihara Y. Applicability of Inter-simple sequence repeats polymorphisms in wheat for use DNA markers in comparison to RFLP and RAPD markers. Theor. Appl. Genet.1997,597-602
[123]江树业,陈启锋,方宣钧.水稻农垦58与农垦58S的RAPD和ISSR分析[J].农业生物技术学报,2000,1(1)：63-66
[124]Fang D Q, Roose ML, Identification of closely related with ISSR markers [J]. Theor. Appl. Genet.1997,95:408-417
[125]Prevost A, Wilkinson M. J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor. Appl. Genet,1999,98:107-112
[126]赵国芳ISSR对梨属栽培品种(Pyrus L.)基因组的指纹分析.河北农业大学硕士论文,2003,5
[127]J.C. Huang, M.Sun. Genetic diversity and relationships of sweet potato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis o chloroplast DNA [J]. Theor Appl Genet,2000,100:1050-1060
[128]Tkojima, T. Nagaoka, K. Nooda. Genetic linkage map of ISSR and RAPD Markers in Einkorn wheat in relation to that of RFLP markers [J]. Theor Appl Genet,1998,96:37-45
[129]席嘉宾,郑玉忠,杨中艺.地毯草ISSR反应体系的建立与优化[J].中山大学学报(自然科学版),2004,25(3)：80-84
[130]王瑜,袁庆华.紫花苜蓿ISSR-PCR反应体系的建立与优化[J].草地学报,2007,15(3)：212-215
[131]尚海英,郑有良,魏育明等.应用ISSR标记研究黑麦草属植物遗传多样性[J].西南农业学报,2004,17(3)：273-277
[132]祁建民,王涛,陈顺辉,周东新等.部分烟草种质遗传多样性与亲缘关系的ISSR标记分析[J].作物学报,2006,32(2)：373-378
[133]穆立蔷,刘赢男,冯富娟等.紫椴ISSR-PCR反应体系的建立与优化[J].林业科学,2006,42(6)：26-31
[134]汪为民.豌豆数量性状的基因效应分析[J].上海农学院学报,1997,15(2)：104-108
[135]杨俊品.豌豆主要农艺性状的配合力分析[J].四川农业大学学报,1996,14(3)：378-381
[136]纪玉贵.食荚豌豆雄性不育突变体的遗传研究[J].华北农学报,2005,20(1)：63-66
[137]王凤宝.豌豆半无叶突变体性状的遗传及在育种上的利用[J].遗传,2003,25(2)：185-188
[138]王凤宝.半无叶型菜豌豆af基因遗传研究及其利用[J].园艺学报,2004,31(6)：747-750
[139]赵秀榆.宁南霉素防治菜豌豆白粉病[J].农药,2000,29(4)：41
[140]陈振落.甜豌豆白粉病的发生与防治[J].福建农业,2004,(2)：20-21
[141]刘爱媛.豌豆离体叶片鉴定白粉病抗性方法[J].植物保护学报,2002,29(2)：119-123
[142]黄旭正.冬种豌豆白粉病及防治方法[J].蔬菜生产,2002,29(2)：12
[143]Davidson J A, Krysinska-Kaczmarek M, Kimber RBE, Ramsey MD. Screening field pea germplasm for resistance to downy mildew (Peronospora viciae) and powdery milderw (Erysiphe pisi)[J]. Australasian Plant Pathology.2004,33(3):413-417
[144]Diego R, Monica FA, Eleonora B, Josefina CS and Sara F. Disease Resistance in pea (Pisum sativum L.) Types for Autumn Sowings in Mediterranean Environments [J]. Plant Breed.2009,45(4):135-142
[145]Dirlewanger E, Isaacp G, Ranade S. Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and development traits in Pisum sativum L.[J]. Appl. Genet.1994,88:17-27
[146]Eklund M, Tuvesson S, Microsat. Ellite markers for powdery mildew resistance in pea Pisum sativum L.[J]. Hereditas.2005,142:86-91
[147]Fondevilla S, Carver TLW, Moreno MT, Rubiales D. Macroscopic and histological characterization of genes erl and er2for powdery mildew resistance in pea [J]. Eur J Plant Pathol.2006,115:309-321
[148]彭化贤,姚革.我国豌豆地方品种抗白粉病性的研究[J].植物病理学报,1990,23：62
[149]仇元.牧草及绿肥作物病害[M].北京：高等教育出版社,1959,419
[150]Wendy C Morgan, Parbery D G. Effects of Pseudopeziza leaf spot disease on growth and yield in Lucerne [J]. Aust J Agric Res,1977,(1):1029-1040
[151]南志标,李春杰.中国牧草真菌病害名录[J].草业科学,1994,(增刊)：611
[152]张蓉,马建华.宁夏苜蓿病虫害发生现状及防治对策[J].草业科学,2003,20(6)：40-44
[153]N.E.希斯,R.F巴恩斯,D.S.梅特卡夫主编.黄文惠,苏加楷,张玉发,等译.牧草-草地农业科学[M].北京：农业出版社,1992.68-86
[154]韩金声.牧草病害[M].北京：北京农业大学出版社,1988,163-165
[155]杨德良,刘有德.43%好力克悬浮剂防治大荚豌豆白粉病锈病田间试验[J].植物保护,2002,3：27-29
[156]王兆美,字迎彪,杨锡银等.30%特富灵粉剂防治大荚豌豆白粉病试验[J].蔬菜,2000,(6)：27
[157]Pammel, L.H. Fungus disease of orange plants [J].Mo Rev.Iowa Weather Crop Serv,1891,2,8-9
[158]Le Clerg, E.C. Crop losses due to plant diseasesin the United States [J]. Phytopathology,1964,54:1309-1313
[159]Anon. Losses in agriculture [M]. U.S. Dep. Agric.Handb.1965,291
[160]肖悦岩,季伯衡,杨之为.植物病害流行与预测[M].北京：中国农业大学出版社,1998,124-137
[161]曾士迈.植保系统工程导论[M].北京：北京农业大学出版社,1994
[162]曾士迈,杨演.植物病害流行学[M].北京：农业出版社,1986,87-117
[163]Willis, C.B. Observations on the disease of forage crops in Prince Edward Island [J]. Can. Plant Dis. Surv.1965,45,8-11
[164]Horsfall, J.G. A study of meadow crop disease in New York [J]. Cornell Univ. Agric. Exp. Stn. Men.,1930,130
[165]曾士迈.著名专家、学者对21世纪我国植物病虫害可持续控制的展望[J].植物保护,1998(6)：35-37
[166]李志勇.牧草种质资源描述规范和数据标准[M].北京：中国农业出版社,2005
[167]明军,顾万春.紫丁香表型多样性研究[J].林业科学研究,2006,19(2)：199-204
[168]竺利波,顾万春,李斌.紫荆群体表型性状多样性研究[J].林业科学,2007,23(3)：138-145
[169]王俊娥,王赞,王运琦,等.山羊豆种质资源形态多样性分析[J].植物遗传资源学报,2008,9(2)：201-205
[170]王志刚.菜用豌豆种质资源形态性状遗传多样性分析.硕士学位论文,中国农业科学院,2009
[171]Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification [J]. Genomics,1994,20:176-183
[172]王瑜,袁庆华.紫花苜蓿ISSR-PCR反应体系的建立与优化[J].草地学报,2007,15(3)：212-215
[173]Godwin I D, Aitken E A, Smith L W. Application of intersimple sequence repeat (ISSR) markers to plant genetics [J]. Electrophoresis,1997,18:1524-1528
[174]顾红雅,瞿礼嘉.植物分子生物学实验手册[M].北京：高等教育出版社,1998
[175]王关林,方宏筠.植物基因工程原理与技术[M].北京：科学出版社,1998
[176]王方,袁庆华.冰草ISSR-PCR反应体系的建立与优化[J].草地学报,2009,17(3)：354-357
[177]袁庆华,张君艳.苜蓿假盘菌ISSR反应体系优化及指纹图谱构建[J].草地学报,2008,16(1)：17-22
[178]卢萍,陈曦,赵萌莉.砂珍棘豆ISSR-PCR反应条件优化研究[J].华北农学报,2008,23(5)：104-106
[179]胡甦,王永清,陶炼,等.三角紫叶酢浆草ISSR反应体系的建立与优化[J].草业学报,2011,20(5)：142-150
[180]冯亮亮,唐红,李毅,等.甘肃红砂不同种群遗传多样性的ISSR分析[J].草业学报,2011,20(1)；125-130
[181]李鸿雁,李志勇,王小丽,等.内蒙古扁蓿豆遗传多样性的ISSR分析[J].西北植物学报,2011,31(1)：0052-0056
[182]王照兰,杨持,赵丽丽,等.扁蓿豆不同品系ISSR标记遗传差异和遗传多样性[J].中国草地学报,2010,32(1)：11-17
[183]耿立格,王丽娜,张磊,等.河北省绿子叶黑豆种质资源表现型和ISSR标记遗传多样性分析[J].植物遗传资源学报,2010,11(3)：266-270
[184]卢萍,赵萌莉,韩国栋,等.内蒙古小花棘豆遗传多样性的ISSR分析[J].西北植物学报,2007,27(6)：1102-1107
[185]宗绪晓,Rebecca Ford, Robert R Redden,等.豌豆属SSR标记遗传多样性结构鉴别与分析[J].中国农业科学,2009,42(1)：36-46
[186]陈永霞,张新全,谢文刚,等.利用EST-SSR标记分析西南扁穗牛鞭草种质的遗传多样性[J].草业学报,2011,20(6)：245-253
[187]王海飞,关建平,马钰,等.中国蚕豆种质资源ISSR标记遗传多样性分析[J].作物学报,2011,37(4)：595-602
[188]刘忠辉,刘国道,黄必志,等.云南猪屎豆属遗传多样性的ISSR分析[J].草业学报,2009,18(5)：184-191
[189]范彦,李芳,张新全,等.扁穗牛鞭草种质遗传多样性的ISSR分析[J].草业学报,2007,16(4)：76-81
[190]周泽扬,夏庆友,鲁成,等.分子系统生物学研究中分子位点数与遗传差异信息可靠性的关系[Jl遗传,1998,20(5)：12-15
[191]陈群航,郑益嫩.福建省豌豆白粉病调查与防治[J].福建农业科技,1998,(5)：11-12
[192]王生荣,陈诚,柴兆祥,等.甘肃省草本植物白粉菌调查(一)[Jl.草原与草坪,2001,(1)：25-27
[193]胡锐,邢彩云,吴营昌,等.11个优质小麦品种对小麦白粉病抗性的初步鉴定[J].河南农业科学,2011,40(5)：108-110
[194]Braun U. The Powdery Mildews (Erysiphales) of Europe. Stuttgart:Gustav Fischer Verlag [M]1995
[195]盖钧镒.试验统计方法[M].北京：中国农业出版社,2000
[196]林成辉,唐乐尘,倪伟健,等.不同豌豆品种对白粉病的抗性特点与防治对策[J].中国蔬菜,2002,(6)：37-38
[197]彭化贤,姚革,贾瑞林,等.豌豆抗白粉病资源鉴定研究[J].西南农业大学学报,1991,13(4)：384-386
[198]Amrish Vaid, P.D. Tyagi豌豆白粉病抗性的遗传学[J].国外农学—杂粮作物,1998,18(5)：14-16
[199]王昶,杨晓明,杨发荣等.陇豌1号在西北豌豆种植区最适密度研究[J].中国种业,2011,(2)：47-48
[200]刘微,刘淑艳,李玉,等.番茄白粉病的病原菌鉴定[J].植物病理学报,2009,39(1)：11-15
[201]邵伯飞,胡东维,李德葆.大豆与白粉病菌相互作用的超微结构与细胞化学[J].电子显微学报,2001,20(6)：744-747
[202]刘东顺,程鸿,孔维萍,等.甘肃甜瓜主产区白粉病菌生理小种的鉴定[J].中国蔬菜,2010,(6)：28-32
[203]王娟,宫国义,郭绍贵,等.北京地区瓜类蔬菜白粉病菌生理小种分化的初步鉴定[J].中国蔬菜,2006,(8)：7-9
[204]邢会琴,李敏权,徐秉良,等.气孔与苜蓿品种对白粉病抗性的关系[J].草原与草坪,2003,(3)：42-45
[205]刘晓玲,宋超,杜文华.红三叶对白粉病抗性机理研究[J].草原与草坪,2011,31(3)：73-76
[206]Ahl-Coy P, Felix G, Metraux JP, et al. Resistance to disease in the hybrid Nicotiana glutinosa x Nicotiana debneyi is associated with high constitutive levels β-1,3-glucanase, chitinas, peroxidase and polyphenoloxidase. Physiol. Mol. Pathol.1992,41:11-21
[207]李靖,利容千,袁文静.黄瓜感染霜霉病菌叶片中一些酶活性的变化[J].植物病理学报,1991,21(4)：277-283
[208]董毅敏,徐建华,郭季芳.抗病和感病黄瓜品种感染白粉菌后几种酶活性的变化[J].植物学报,1990,32(2)：160-164
[209]李明岩,屈淑平,崔崇士.利用酶活性鉴定南瓜对白粉病菌抗病性的研究[J].东北农业大学学报,2007,38(6)：737-741
[210]李惠霞,王蒂.马铃薯晚疫病抗性反应中木质素及防御酶活性的变化[J].甘肃农业大学学报,2006,41(3)：52-56
[211]周晓慧,Wolukau JN,李英,等.甜瓜蔓枯病抗性与SOD、CAT、和POD活性变化的关系[J].中国瓜菜,2007,(2)：4-6
[212]云兴福,崔世茂,霍秀文.黄瓜组织中几种酶活性与其对霜霉病抗性的关系lJl.华北农学报,1995,10(1)：92-98
[213]孔凡明,许志刚.水稻不育系抗白枯病与体内酶活性变化的关系lJl.安徽农业大学学报,1998,25(3)：217-223
[214]邹琦.植物生理学实验指导[M].北京：中国农业出版社,2000,163-164
[215]王保成,孙万仓,范惠玲,等.芸芥自交亲和系与自交不亲和系SOD、POD和CAT酶活性lJl.中国油料作物学报,2006,28(2)：162-165
[216]张妙霞,孔祥生.蒽酮法测定可溶性糖显色条件的研究[J].洛阳农专学报,1997,17(4)：24-28
[217]邹琦.植物生理学实验指导[M].北京：中国农业出版社,2000,111-112
[218]邹琦.植物生理学实验指导[M].北京：中国农业出版社,2000,129-130
[219]邹琦.植物生理学实验指导[M].北京：中国农业出版社,2000,72-75
[220]毛晓英,张玉岭,李茂盛,等.BTH对新疆甜瓜抗病性的诱导研究[J].新疆农业科学,2005,42(3)：158-161
[221]Graham MY, Graham TL. Rapid accumulation of anionic peroxidases and phenolic polymers in soybean cotyledon tissues following treatment with Phytophthora megasperma F. sp. Glycinea wall glucan[J]. Plant Physiol.1991,97:1445-1455
[222]王国梁,卢浩然,陈启峰.水稻品种抗瘟性生化鉴定的研究[J].福建农学院学报,1986,15(3)：195-203
[223]董汉松.植物诱导抗病性原理与研究[M].北京：科学出版社,1995,160-170
[224]李合生,李琳,焦新之.现代植物生理学实验指南[M].北京：科学出版社,1999,164-165
[225]潘瑞炽.植物生理学(第四版)[M].北京：高等教育出版社,2001,112
[226]贺立红,宾金华.高等植物中的多酚氧化酶[J].植物生理学通讯,2001,37(4)：340-345
[227]张欣.与植物抗病性有关酶的研究进展[J].华南热带农业大学学报,2000,6(1)：41-46
[228]云兴福.黄瓜组织中氨基酸、糖和叶绿素含量与其对霜霉病抗性的关系[J].华南农学报,1993,8(4)：52-58
[229]刘素萍,王汝贤.根系分泌物中糖和氨基酸对棉花枯萎菌的影响[J].西北农业大学学报,1998,26(6)：30-31
[230]龙书生,李亚玲.糖分含量作为抗镰刀菌茎腐病玉米品种的育种指标研究[J].山东农业大学学报,1999,30(4)：372-380
[231]骆桂芬,崔俊涛.黄瓜叶片中糖和木质素含量与霜霉病诱导抗性的关系[J].植物病理学报,1997,27(1)：65-69
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |