甘蔗生物固氮量评估及固氮甘蔗品种的氮肥运筹效应研究
|
摘要
甘蔗植株高大,生长周期长,生长期间需要消耗大量养分。在甘蔗生产的各项农资投入中,化肥尤其是氮肥所占比重最大,对甘蔗产量的影响也最显著。我国甘蔗栽培氮肥施用量一般达500-750kg/ha,远远超过其他国家的平均施氮水平275kg/ha,不仅生产成本高,生产收益低,还影响生态环境。若能减少氮肥施用量,则可降低生产成本,提高生产收益,保护和改善生态环境,促进甘蔗糖业可持续发展。
桂引5号(RB72-454)、桂引6号(B8)是近年引进的巴西固氮甘蔗新品种。这两个品种在广西的栽培环境条件下仍具有较强的生物固氮能力。为了更好地利用它们的生物固氮特性,寻求生产上科学施用氮肥的依据和方法,本研究分别采用15N同位素稀释法和15N自然丰度法,以木薯为参比植物,进行温室大棚试验,测定了桂引6号的生物固氮百分率和固氮量,区分了桂引6号植株总氮量中来自空气氮、肥料氮和土壤氮的氮量比；采用U207均匀设计、田间试验方法,研究了施氮量和止氮期对桂引5号、桂引6号蔗茎产量和蔗糖产量的影响效应,建立了二元二次回归模型,提出了广西蔗区实现高产高糖的施肥建议。主要结果如下：
1.用15N自然丰度法测定甘蔗根、茎、叶的δ15N值,其大小依次为18.13‰、17.02‰和13.66‰；甘蔗叶的δ15N值极显著低于根和茎。用叶的615N值计算出甘蔗固氮百分率为24.47%。
2.用15N同位素稀释法测定甘蔗植株全氮量中来自空气氮、肥料氮及土壤氮的比例,其值分别为11.35%、7.69%、80.96%。测得甘蔗根、茎、叶的固氮百分率大小依序为：叶(13.27%)>茎(8.51%)>根(4.34%),叶的固氮能力最强。
3.用15N同位素稀释法测得甘蔗的氮肥利用率为58.76%。
4.施氮量和止氮期对固氮甘蔗品种蔗茎产量、蔗糖分和蔗糖产量有影响。施氮量是主要影响因素,止氮期是次要影响因素。施氮量对蔗茎产量、蔗糖分和蔗糖产量起直接作用,表现出有一个最佳作用范围；在这个范围内,蔗茎产量、蔗糖分和蔗糖产量达到最大值；超出这个范围,蔗茎产量、蔗糖分和蔗糖产量均降低。施氮量还通过与止氮期配合间接影响蔗茎产量、蔗糖分和蔗糖产量。止氮期对蔗茎产量、蔗糖分和蔗糖产量的影响较为复杂,在不同甘蔗品种上有不同表现。
5.在广西蔗区耕型赤红壤中等肥力条件下,固氮甘蔗品种桂引5号冬植蔗高产高糖的氨肥运筹方案及施肥建议是：施氮量为纯氨225～247.5kg/ha,止氮期为植后90d(分蘖初期)。按照此施肥方案,可实现现目标蔗茎产量92.25t/ha、目标蔗糖产量12.62t/ha。
6.在广西蔗区耕型赤红壤中等肥力条件下,固氮甘蔗品种桂引6号高产高糖栽培,其一年新植、两年宿根的分年度施肥议案如下：
新植蔗：：施氮量为180kg/ha；止氮期为植后210d(旺盛伸后期)。预报蔗茎产量124.05t/ha、蔗糖分15.01%、蔗糖产量18.46t/ha。
第一年宿根蔗：施氮量为135kg/ha；止氮期为破垄松蔸后90d(出苗末期至分蘖初期)。预报蔗茎产量93.00t/ha、蔗糖分13.85%、蔗糖产量13.35t/ha。
第二年宿根蔗：施氮量为45kg/ha；止氮期为破垄松蔸后90d(出苗末期至分蘖初期)。预报蔗茎产量111.29t/ha、蔗糖分12.29%、蔗糖产量13.75t/ha。
Sugarcane is a gramineous grass with tall plant and long growth duration, and needs to consume massive nutrients during growth. Fertilizer, especially nitrogen, occupies the largest proportion in sugarcane production inputs and affects cane yield most significantly. The application rate of nitrogen fertilizer in sugarcane production in China amounts to pure nitrogen500-750kg/ha, which is much more than that in other countries, that is275kg/ha on an average, resulting in not only high production costs and low production gains, but also environmental pollution. Reducing the amount of nitrogen fertilizer application will lead to decrease in production costs, increase in net income, also be good to protect and improve the ecological environment, and assure sustainable development of cane sugar industry.
Guiyin5(RB72-454) and Guiyin6(B8) are the nitrogen-fixing sugarcane varieties introduced from Brazil in recent years. These two varieties have strong biological nitrogen fixation ability under the environmental conditions in Guangxi. The present study was conducted in order to make a better understanding on their biological nitrogen fixation characteristics and to set up a scientific basis for application of nitrogen fertilizer in commercial sugarcane production. The15N isotope dilution method and the15N natural abundance method were employed to determine the biological nitrogen-fixation percentage and quantity in sugarcane with cassava as a reference plant in greenhouse, and to distinguish the nitrogen rates from air, fertilizer and soil, respectively in total nitrogen of the plant of the sugarcane variety Guiyin6. U207uniform design and field experimental methods were used to study the effects of nitrogen rate applied and time for stopping nitrogen application on cane yield and sugar content in sugarcane varieties Guiyin5and Guiyin6. The bivariate regression models were established and the fertilization recommendations to achieve high yield and high sugar was raised. The main results are as follows.
1. The δ15N value of the roots, stems and leaves from sugarcane plant were determined by15N natural abundance method with the value records18.13%、17.02%、and13.66%。in proper sequence. The δ15N value of sugarcane leaves was very significantly lower than that of the roots and stems. The sugarcane nitrogen-fixing percentage was24.47%calculated based the δ15N value of sugarcane leaves.
2. The nitrogen rate in total nitrogen of sugarcane plant from air, fertilizer and soil respectively were11.35%、7.69%、80.96%determined by15N isotope dilution method. The nitrogen-fixing percentage of sugarcane roots, stems and leaves were follower in order leaf (13.27%)> stem (8.51%)> root (4.34%),with the nitrogen-fixing ability of sugarcane leaves most powerful.
3. The nitrogen use efficiency for sugarcane was58.76%determined by15N isotope dilution method.
4. The nitrogen rate applied and time for stopping nitrogen application effected the cane yield and sugar content of the nitrogen-fixing sugarcane varieties. Nitrogen rate applied was the main factor and the time for stopping nitrogen application was subordinated. The nitrogen rate applied showed a significant direct effect on cane yield and sugar content which reached to the maximum with increase in certain extent, but both were decreased beyond the scope of the best range. Nitrogen rate applied can also affect the cane yield and sugar content indirectly combined with time for stopping nitrogen application. The effects of time for stopping nitrogen application on cane yield and sugar content is more complex with different performances in different sugarcane varieties.
5. Under the conditions of cultivation-red soil with medium-fertility in Guangxi sugarcane crop area, in order to get the higher sugarcane yield and higher sugar yield in winter planting, the following nitrogen fertilizer application strategy plan was been adviccd for Guiyin5, sugarcane variety with nitrogen-fixation ability:the nitrogen rate applied (purity nitrogen)225-247.5kg/ha and the time for stopping nitrogen application at90d after planting (the early tillering stage). The sugarcane yield can be expected92.25t/ha and the sugar yield12.62t/ha in this nitrogen fertilizer application strategy plan.
6. Under the conditions of cultivation-red soil with medium-fertility in Guangxi sugarcane crop area, the nitrogen fertilizer application strategy plan for new planting and ratoon were follows for higher sugarcane yield and higher sugar yield in winter planting of Guiyin6, another sugarcane variety with nitrogen-fixation ability
The new plant sugarcane:the nitrogen rate applied (purity nitrogen) was180kg/ha and the time for stopping nitrogen application at210d after planting (the later of exuberant elongation). The aim forecasting as follows:the sugarcane yield can be expected124.05t/ha, the sucrose15.01%and the sugar yield18.46t/ha.
The first year ratoon-cane:the nitrogen rate applied (purity nitrogen) was135kg/ha and the time for stopping nitrogen application at90d after breaking loose hills ridges(from the end of germinate again to the early tillering stage). It were been forecasted that the sugarcane yield93.00t/ha, the sucrose13.85%and the sugar yield13.35t/ha.
The second year ratoon-cane:the nitrogen rate applied (purity nitrogen) was45kg/ha and the time for stopping nitrogen application at90d after breaking loose hills ridges(from the end of germinate again to the early tillering stage). It were been forecasted that the sugarcane yield111.29t/ha, the sucrose12.29%and the sugar yield13.75t/ha.
桂引5号(RB72-454)、桂引6号(B8)是近年引进的巴西固氮甘蔗新品种。这两个品种在广西的栽培环境条件下仍具有较强的生物固氮能力。为了更好地利用它们的生物固氮特性,寻求生产上科学施用氮肥的依据和方法,本研究分别采用15N同位素稀释法和15N自然丰度法,以木薯为参比植物,进行温室大棚试验,测定了桂引6号的生物固氮百分率和固氮量,区分了桂引6号植株总氮量中来自空气氮、肥料氮和土壤氮的氮量比；采用U207均匀设计、田间试验方法,研究了施氮量和止氮期对桂引5号、桂引6号蔗茎产量和蔗糖产量的影响效应,建立了二元二次回归模型,提出了广西蔗区实现高产高糖的施肥建议。主要结果如下：
1.用15N自然丰度法测定甘蔗根、茎、叶的δ15N值,其大小依次为18.13‰、17.02‰和13.66‰；甘蔗叶的δ15N值极显著低于根和茎。用叶的615N值计算出甘蔗固氮百分率为24.47%。
2.用15N同位素稀释法测定甘蔗植株全氮量中来自空气氮、肥料氮及土壤氮的比例,其值分别为11.35%、7.69%、80.96%。测得甘蔗根、茎、叶的固氮百分率大小依序为：叶(13.27%)>茎(8.51%)>根(4.34%),叶的固氮能力最强。
3.用15N同位素稀释法测得甘蔗的氮肥利用率为58.76%。
4.施氮量和止氮期对固氮甘蔗品种蔗茎产量、蔗糖分和蔗糖产量有影响。施氮量是主要影响因素,止氮期是次要影响因素。施氮量对蔗茎产量、蔗糖分和蔗糖产量起直接作用,表现出有一个最佳作用范围；在这个范围内,蔗茎产量、蔗糖分和蔗糖产量达到最大值；超出这个范围,蔗茎产量、蔗糖分和蔗糖产量均降低。施氮量还通过与止氮期配合间接影响蔗茎产量、蔗糖分和蔗糖产量。止氮期对蔗茎产量、蔗糖分和蔗糖产量的影响较为复杂,在不同甘蔗品种上有不同表现。
5.在广西蔗区耕型赤红壤中等肥力条件下,固氮甘蔗品种桂引5号冬植蔗高产高糖的氨肥运筹方案及施肥建议是：施氮量为纯氨225～247.5kg/ha,止氮期为植后90d(分蘖初期)。按照此施肥方案,可实现现目标蔗茎产量92.25t/ha、目标蔗糖产量12.62t/ha。
6.在广西蔗区耕型赤红壤中等肥力条件下,固氮甘蔗品种桂引6号高产高糖栽培,其一年新植、两年宿根的分年度施肥议案如下：
新植蔗：：施氮量为180kg/ha；止氮期为植后210d(旺盛伸后期)。预报蔗茎产量124.05t/ha、蔗糖分15.01%、蔗糖产量18.46t/ha。
第一年宿根蔗：施氮量为135kg/ha；止氮期为破垄松蔸后90d(出苗末期至分蘖初期)。预报蔗茎产量93.00t/ha、蔗糖分13.85%、蔗糖产量13.35t/ha。
第二年宿根蔗：施氮量为45kg/ha；止氮期为破垄松蔸后90d(出苗末期至分蘖初期)。预报蔗茎产量111.29t/ha、蔗糖分12.29%、蔗糖产量13.75t/ha。
Sugarcane is a gramineous grass with tall plant and long growth duration, and needs to consume massive nutrients during growth. Fertilizer, especially nitrogen, occupies the largest proportion in sugarcane production inputs and affects cane yield most significantly. The application rate of nitrogen fertilizer in sugarcane production in China amounts to pure nitrogen500-750kg/ha, which is much more than that in other countries, that is275kg/ha on an average, resulting in not only high production costs and low production gains, but also environmental pollution. Reducing the amount of nitrogen fertilizer application will lead to decrease in production costs, increase in net income, also be good to protect and improve the ecological environment, and assure sustainable development of cane sugar industry.
Guiyin5(RB72-454) and Guiyin6(B8) are the nitrogen-fixing sugarcane varieties introduced from Brazil in recent years. These two varieties have strong biological nitrogen fixation ability under the environmental conditions in Guangxi. The present study was conducted in order to make a better understanding on their biological nitrogen fixation characteristics and to set up a scientific basis for application of nitrogen fertilizer in commercial sugarcane production. The15N isotope dilution method and the15N natural abundance method were employed to determine the biological nitrogen-fixation percentage and quantity in sugarcane with cassava as a reference plant in greenhouse, and to distinguish the nitrogen rates from air, fertilizer and soil, respectively in total nitrogen of the plant of the sugarcane variety Guiyin6. U207uniform design and field experimental methods were used to study the effects of nitrogen rate applied and time for stopping nitrogen application on cane yield and sugar content in sugarcane varieties Guiyin5and Guiyin6. The bivariate regression models were established and the fertilization recommendations to achieve high yield and high sugar was raised. The main results are as follows.
1. The δ15N value of the roots, stems and leaves from sugarcane plant were determined by15N natural abundance method with the value records18.13%、17.02%、and13.66%。in proper sequence. The δ15N value of sugarcane leaves was very significantly lower than that of the roots and stems. The sugarcane nitrogen-fixing percentage was24.47%calculated based the δ15N value of sugarcane leaves.
2. The nitrogen rate in total nitrogen of sugarcane plant from air, fertilizer and soil respectively were11.35%、7.69%、80.96%determined by15N isotope dilution method. The nitrogen-fixing percentage of sugarcane roots, stems and leaves were follower in order leaf (13.27%)> stem (8.51%)> root (4.34%),with the nitrogen-fixing ability of sugarcane leaves most powerful.
3. The nitrogen use efficiency for sugarcane was58.76%determined by15N isotope dilution method.
4. The nitrogen rate applied and time for stopping nitrogen application effected the cane yield and sugar content of the nitrogen-fixing sugarcane varieties. Nitrogen rate applied was the main factor and the time for stopping nitrogen application was subordinated. The nitrogen rate applied showed a significant direct effect on cane yield and sugar content which reached to the maximum with increase in certain extent, but both were decreased beyond the scope of the best range. Nitrogen rate applied can also affect the cane yield and sugar content indirectly combined with time for stopping nitrogen application. The effects of time for stopping nitrogen application on cane yield and sugar content is more complex with different performances in different sugarcane varieties.
5. Under the conditions of cultivation-red soil with medium-fertility in Guangxi sugarcane crop area, in order to get the higher sugarcane yield and higher sugar yield in winter planting, the following nitrogen fertilizer application strategy plan was been adviccd for Guiyin5, sugarcane variety with nitrogen-fixation ability:the nitrogen rate applied (purity nitrogen)225-247.5kg/ha and the time for stopping nitrogen application at90d after planting (the early tillering stage). The sugarcane yield can be expected92.25t/ha and the sugar yield12.62t/ha in this nitrogen fertilizer application strategy plan.
6. Under the conditions of cultivation-red soil with medium-fertility in Guangxi sugarcane crop area, the nitrogen fertilizer application strategy plan for new planting and ratoon were follows for higher sugarcane yield and higher sugar yield in winter planting of Guiyin6, another sugarcane variety with nitrogen-fixation ability
The new plant sugarcane:the nitrogen rate applied (purity nitrogen) was180kg/ha and the time for stopping nitrogen application at210d after planting (the later of exuberant elongation). The aim forecasting as follows:the sugarcane yield can be expected124.05t/ha, the sucrose15.01%and the sugar yield18.46t/ha.
The first year ratoon-cane:the nitrogen rate applied (purity nitrogen) was135kg/ha and the time for stopping nitrogen application at90d after breaking loose hills ridges(from the end of germinate again to the early tillering stage). It were been forecasted that the sugarcane yield93.00t/ha, the sucrose13.85%and the sugar yield13.35t/ha.
The second year ratoon-cane:the nitrogen rate applied (purity nitrogen) was45kg/ha and the time for stopping nitrogen application at90d after breaking loose hills ridges(from the end of germinate again to the early tillering stage). It were been forecasted that the sugarcane yield111.29t/ha, the sucrose12.29%and the sugar yield13.75t/ha.
引文
[1]安千里,李久蒂.植物内生固氮菌.植物生理学通讯,1999,35(4)：265-272
[2]陈瑞邦,张必雍,陈业贞,等.旱地甘蔗终止施氮期试验总结.广西农学院学报,1987,(1)：13-15
[3]蔡开地,罗凤来,黄梅卿.春花生施用钙镁硼肥效应函数与分析.植物营养与肥料学报,2002,8(2)：248-250
[4]陈朝勋,席琳乔,姚拓,等.生物固氮测定方法研究进展.草原与草坪,2005,(2)：24-26
[5]陈超君,向立华,宋日云,等.施肥技术对甘蔗产量和品质的影响.广西农业生物科学,2007,26(6)：91-95
[6]陈春,张兴祥,黄可立,等.甘蔗高产栽培模式研究.华南农业大学学报,1991,12(4)：45-51
[7]陈建平,刘伟仲,顾双平.氮肥与化控配合应用对棉花主要经济性状的影响.江苏农业科学1990,(4)：17-19,38
[8]陈建平,顾双平,刘伟仲.氮肥与化控配合应用对棉花产量和品质的影响.棉花学报,19935(2)：49-54
[9]陈明,姚允寅,张希忠.不同形态氮素对15N同位素稀释估测苜蓿固氮的影响.核农学报,1997,18(1)：30-33,40
[10]陈祥龙,李秀章、徐立华,等.小麦后移棉的氮肥、密度效应及器官建成动态研究.棉花学报,1995,7(1)：41-45
[11]陈玉水.种源甘蔗的氮肥需要量和适宜施用期研究.广西蔗糖,2003,33(4)：47-49
[12]戴建军,程岩.应用15N示踪技术对不同品种大豆的三种氮源吸收利用的研究.东北农业大学学报,1999,30(3)：225-229
[13]东秀珠,蔡妙英.常见细菌系统鉴定手册.北京：北京科学出版社,200],128
[14]窦新田.生物固氮.北京：中国农业出版社,1989.17-19
[15]杜丽娟,施书莲,周克瑜,等.应用15N自然丰度法测定固氮植物的固氮量Ⅲ.参比植物的选择.土壤,1996,28(4)：210-212
[16]杜旭华,彭方仁,周贤军.植物的联合固氮机理及研究现状.林业科技情报,2006,38(4)：1-3
[17]方开泰.均匀设计.应用数学学报,1980,3(4)：363-372
[18]樊妙姬,陈丽梅,蒋艳明.甘蔗联合固氮菌对甘蔗叶片养分含量的影响.广西科学,1998,5(4)：311-313
[19]冯海强,潘志强,于翠平,等.利用¨N自然丰度法鉴别有机茶的可行性分析.核农学报,2011,25(2)：0308-0312
[20]龚得名,林彦铨,陈如凯,等.甘蔗不同密度群体的几个光合性状及产量的比较研究.福建农科院学报,1994,9(2)：40-49
[2l]桂意云.不同氮水平对甘蔗生物固氮活性的影响[硕士学位论文].南宁：广西大学,2007
[22]桂意云,刘昔辉,杨荣仲,等.甘蔗不同部位的固氮酶活性检.植物生理学通讯,2007,43(2)：291-294
[23]韩筱渝,李军梅.固氮品种桂引5号种性研究.广西蔗糖,2006,(2)：3-6,11
[24]何道文,孙辉,黄雪菊.利用N-15自然丰度法研究固氮植物生物固氮量.干旱地区农业研究,2004,22(1)：132-137
[25]何天春,岑黄源,覃晓远.甘蔗高产优质不同氮肥量的施用效应.广西蔗糖,2006,45(4)：31-33
[26]黄东风,翁伯琦,罗涛.豆科植物固氮能力的主要测定方法比较.江西农业大学学报,2003,(25)：17-20
[27]黄玉溢,谭宏伟,周柳强,等.几种长效氮肥对甘蔗的效应.广西农学报,2003,(S1)：76-78
[28]蓝立斌,陈超君,米超,等.不同施氮量对甘蔗生理性状、产量和品质的影响.广西农业科学,2010,41(12)：1269-1272
[29]蓝立斌,陈超君,米超,等.不同施氮方式对甘蔗生理生化性状的影响.南方农业学报,2011,42(1)：26-29
[30]李海先,李新民,Seth K A Danso.应用15N稀释法筛选高固氮能力的大豆品种.核农学报,1998,12(5)：299-303
[31]李香真,陈清.”N同位素稀释法测定生物固氮量.核农学通报.1997,(6)：291-293,271
[32]李星洪,李大捷,李薇,等.对从甘蔗根部分离的固氮成团泛菌的研究.北京大学学报(自然科学版),1998,34(6)：741-745
[33]李杨瑞主编.现代甘蔗学.北京：中国农业出版社,2010,85-102,218-263,313-333
[34]李永兴,董越梅,李久蒂.联合固氮菌E57-7泌铵突变株的分离和特性.植物学报,1996,38(7)：563-567
[35]李玉中,Redmann R E,祝廷成,等.羊草草原豆科牧草生物固定量研究.草地学报,2002,10(3)：164-166
[36]李玉潜,谭中文,梁计南.甘蔗对涂层尿素的氮素利用率研究.化肥工业,1994,(6)：24-25
[37]李玉潜,谢九生.甘蔗叶片碳、氮代谢与产量、品质关系研究初探.中国农业科学,1995,28(4)：46-53
[38]梁计南,谭中文,陈真元,等.甘蔗不同施氮方法与产量及糖分的关系.甘蔗,2000,7(4)：1-10
[39]梁俊.不同甘蔗品种的固氮生理特性[硕士学位论文].南宁：广西大学,2004
[40]梁俊,梁朝旭.甘蔗内生固氮的研究进展.广西蔗糖,2006,(2)：25-27
[41]梁俊,李杨瑞,梁朝旭.不同甘蔗品种在无氮肥条件下的光合生理特性.中国农学通报,2005,21(8)：188-207
[42]林昌华,高志红,陈晓远,等.不同施肥水平对粤北坡岗地宿根糖蔗干物质积累和产量的影响.广东农业科学,2010,(6)：63-64
[43]林丽,张新成,李杨瑞,等.甘蔗器官固氮酶活性及其对接种固氮菌的响应.西北植物学报, 2008,28(12)：2472-2477
[44]林敏,尤崇杓.根际联合固氮作用的研究进展. 植物生理通讯,1992,28(5)：323-329
[45]廖明坛,陈克文.供N水平对甘蔗生长和产量的影响.福建农业大学学报,1995,24(2)：195-200
[46]刘光栋,吴文良.高产农田土壤硝态氮淋失与地下水污染动态研究.中国生态农业学报,2003,11(1)：91-93
[47]刘希蝶.旱地甘蔗地膜覆盖、不同施肥次数对比试验初报、甘蔗,1994,1(3)：49-50
[48]刘昔辉.甘蔗内生固氮菌的分离鉴定及不同氮水平对其固氮活性影响[硕士学位论文].广西大学,2008
[49]陆国盈,韩世健,杨培忠,不同施氮量对新台糖16号的产量和品质的影响.广西蔗糖,2001.(4)：3-6
[50]陆国盈,韦晖,蒋明明,等.冰水浸种对不同甘蔗品种生理生化特性的影响.广西农业科学,2010,47(2)：113-116
[51]陆世宏,霍润丰.高旱蔗区地膜覆盖不同施肥次数试验.广西甘蔗,1993,(2)：9-11
[52]陆章流.提高宿根蔗低产低糖的技术措施.广西蔗糖,2005,(1)：3-5
[53]罗霆,欧阳雪庆,杨丽涛,等.1株有固氮能力的甘蔗克雷伯氏菌的分离鉴定及固氮特性.热带作用物报,2010b.31(6)：972-978
[54]罗霆,欧阳雪庆,杨丽涛,等.15N同位素稀释法研究固氮菌接种对甘蔗生物固氮的影响.核农学报,2010a,24(5)：1026-1031
[55]罗霆.甘蔗内生固氮与甘蔗互作的研究[博士学位论文].南宁：广西大学,2010
[56]蒙世欢.广西甘蔗施肥现状、问题及对策.广西农学报,2007,22(5)：37-39
[57]欧阳雪庆,罗霆,杨丽涛,等.甘蔗内生固氮菌液浸种对甘蔗生长前期氮代谢相关酶活性的影响.广西农业科学,2010,41(5)：416-418
[58]覃丽萍,黄思良,李杨瑞.植物内生固氮菌的研究进展.中国农学通报,2005,21(2)：150-152.
[59]山崎肯著.刘步洲,刘宜生,安志信等译.营养液栽培大全.北京：中国农业大学出版社1989：24-25
[60]苏波,韩兴国,黄建辉.15N自然丰度法在生态系统氮索循环研究中的应用.生态学报,1999,19(3)：408-416
[61]苏俊波.甘蔗内生固氮菌的分离与鉴定[硕士学位论文].南宁;广西大学,2006
[62]苏俊波,杨荣仲,桂意云,等.甘蔗体内具有固氮酶活性细菌的分离、鉴定及其相关特性研究.西南农业学报,2007,20(5)：1055-1059
[63]谭裕模,王伦旺,王天算,等.固氮甘蔗品种RB72-454田间肥料敏感试验.广西蔗糖,2002,26(1)：12-15
[64]谭中文,李玉潜,梁计南,等.甘蔗高产高糖高效益综合栽培技术中几种主要措施组配的研究.甘蔗糖业,1994,(2)：11-17
[65]王桂文,李海鹰,范嘉晔.甘蔗内生固氮菌研究.广西农业生物科学,2000,19(1)：68-72
[66]王伦旺,李杨瑞,谭裕模,等.巴西甘蔗品种在广西的种性表现及利用前景.甘蔗糖业,2005a,(5)：1-7
[67]王伦旺,谭裕模,李杨瑞,等.甘蔗品种桂引5号种性研究.中国糖料,2005b,(4)：11-15
[68]王伦旺,李杨瑞,何为中,等.以茎尖组培苗检测甘蔗体内内生固氮菌的固氮活性.植物生理学通讯,2007,43(1)：65-68
[69]王伦旺,李杨瑞,杨荣仲,等.不同甘蔗基因型对低氮胁迫的响应.西南农业学报,2010,23(2)：508-514
[70]王伦旺,杨荣仲,李杨瑞,等.糖能兼用固氮甘蔗品种桂引6号的种性研究.广东农业科学,2011,(12)：20-25
[71]韦东普,白玲玉,华珞,等.用15N同位素稀释法研究牧草的氮素营养.核农学报,2000,14(2)：104-109
[72]韦光旺.早地甘蔗二次施肥试验示范.广西甘蔗,1997,8(3)：17-19
[73]韦剑锋,罗艺,米超,等.施氮次数对甘蔗干物质积累、产量及品质的影响.广东农业科学,2011,(20)：57-59
[74]韦莉萍,李杨瑞,满若军,等.钼对甘蔗氮代谢的影响(英文).广西农业科学,2006,37(6)：648-655
[75]韦莉萍,李杨瑞,杨丽涛.钼对甘蔗体内固氮菌的固氮酶活性的影响.植物营养与肥料学报,2007,13(1)：118-122
[76]文建成,陈学宽,符菊芬,等.质膜透性与丙二醛(MDA)含量的变化.甘蔗,1998,(3)：1-5
[77]吴凯朝,梁俊,罗霆,等.接种内生固氮菌对不同基因型甘蔗农艺特性的效应.广西农业科学,2010,41(6)：528-530
[78]吴凯朝,梁俊,韦莉萍,等.内生固氮菌对甘蔗伸长期光合生理特性的影响.广西植物,2011,31(5)：668-673
[79]席琳乔,张德罡,姚拓.15N同位素稀释法测定燕麦根际固氮菌固氮量的研究.核农学报,2007,21(4)：417-420
[80]邢光熹.同位素自然丰度变异及其在环境污染研究中的应用.核农学通报,1981,(2)：1-6
[81]邢永秀.甘蔗内生固氮细菌的分离鉴定和生长特性[博士学位论文].南宁：广西大学,2006
[82]邢永秀,杨丽涛,李杨瑞.巴西固氮甘蔗品种和广西主栽甘蔗品种氮代谢差异比较.安徽农业科学,2008a,36((21)：9003-9007
[83]邢永秀,杨丽涛,黄思良,等.甘蔗茎内两个内生菌株16S rRNA序列分析及其生长特性.微生物学通报,2008b,35(11)：1732-1737
[84]徐福利,张金水,吕殿青.对苜蓿共生固氮量测定法的比较研究.土壤通报,1993,24(3)：137-139
[85]徐继,李久蒂.生物固氮.生物学通报,1997,32(6)：16-18
[86]徐健,莫小燕,陈远权.等.配方施肥对甘蔗生长及产量的影响.广西热带农业,2010,(4)：24-26.
[87]徐建云,陆耀邦,不同生育期止肥对甘蔗新植和宿根的效应.甘蔗糖业,1991,(2)：11-15
[88]徐林,黄海荣,黄玉溢,等.地下滴灌条件下氮磷钾配施对甘蔗产量及其构成因素的影响.广西农业科学,2010,41(8)：800-803
[89]徐媛,莫建文,吴少维,陈超君.施氮量和氮肥运筹模式对甘蔗前期生长的影响.中国糖料,2011,(1)：20-22,24
[90]许志斌,沈强云,王承莲,等.春小麦高产高效氮磷经济施肥指标研究.宁夏农学院学报,1997,18(1)：73-77
[91]杨荣仲,谭裕模,李杨瑞.甘蔗固氮种质资源筛选研究.2005植物分子育种国际学术研讨会论文集,2005.157-160
[92]杨荣仲,谭裕模,桂意云,等.15N测定甘蔗生物固氮能力研究.安徽农业科学,2008,36(24)：10405-10406
[93]姚拓,蒲小鹏,张德罡,等.高寒地区燕麦根际联合固氮菌研究Ⅲ 固氮菌对燕麦生长的影响及其固氮量测定.草业学报,2004,13(5)：10-15
[94]姚允寅.应用15N进行土壤肥料和植物营养研究的有关数学运算.核农学通报,1988,(8)：186-189
[95]姚允寅,陈明,王志东,等.百里荒牧场饲草15N天然丰度值的测定及其固氮能力初评.中国草地,1992a,(2)：20-24.
[96]姚允寅,陈明,马昌燐,等.几种饲用牧草的δ15N值及其固氮能力初评.同位素,1992b,5(3)：129-134
[97]姚允寅, 陈明,张希忠,等.用15N同位素稀释法评估诱发结瘤春小麦的固氮作用.中国核科技报告,1994,(00)：1-10
[98]尤崇杓,姜涌明,宋鸿遇.生物固氮.北京：科学出版社,1987,228-242
[99]于佰双.应用15N同位素稀释法鉴定大豆新品系的固氮能力.核农学通报,1997,18(1)：34-35
[]00]古新华,将延惠,徐阳春,等.微生物制剂促进植物生长机理的研究进展.植物营养与肥料学报,1999,5(2)：97-105
[101]张朝春,江荣风,张福锁,等.马铃薯氮、磷、钾肥料效应的研究.中国马铃薯,2004,18(6)：326-329
[102]张丽梅.高效联合固氮菌的筛选、鉴定及其生理生化特性和接种效应研究[博士学位论文].杭州：浙江大学,2002
[104]张丽梅,方萍,朱日清.禾本科植物联合固氮研究及其应用现状展望.应用生态学报,2004,15(9)：1650-1654
[105]赵淑清,田春杰,何兴元.固氮植物的菌根研究.应用生态学报,2000,11(2)：306-310
[106]周汉仁.甘蔗不同施氮期对产量和蔗糖份的影响.甘蔗糖业,1984,(9)：26-28
[107]周克瑜,施书莲,杜丽娟,等.豆科固氮植物植株茎叶、根和根瘤的δ15N值变异.核农学报,1998,12(2)：105-111
[108]周修冲,刘国坚,姚建武,等.旱地甘蔗提高氮肥利用率的施肥技术研究.土壤肥料,2000,(6)：42-43
[109]周正邦,龚德勇,易代勇,等.不同施氮水平对固氮甘蔗品种RB72-454的肥效响应.贵州农业科学,2006,34(1)：52-55
[110]周正邦,龚德勇,易代勇,等.4个甘蔗新品种田间氮肥敏感性试验.贵州农业科学,2004,32(5)：69-70
[111]周正邦,易代勇,龚德勇,等.氮肥对高糖甘蔗品种的增效作用.贵州农业科学,2009,37(8)：65-66
[112]褚贵新,沈其荣,张娟,等.用15N富积标记和稀释法研究旱作水稻/花生间作系统中氮素固定和转移.植物营养与施肥学报,2003,9(4)：385-389
[113]Ando S, Meunchang S, Thippayarugs S, et al. Role of nitrogen fixation for sustainable sugarcane production. In:Li Y R, Solomon S, eds. Sustainable Sugarcane and Sugar Production Technology. Beijing:China Agriculture Press,2004,156-159
[114]Andre M. Atmospheric nitrogen is a reliable standard for natural 15N abundance measurements. Nature,1983,303(23):685-687
[115]Asis Jr C A, Kubota M, Ohta H, et al. Isolation and partial characterization of endophytic diazotrophs associated with Japanese sugarcane cultivar. Soil Science and Plant Nutrition,2000, 46(3):759-765
[116]Balandreau J. Non-symbiotic nitrogen fixation in rhizosphere of rice maize and different. Nitrogen Fixation,1980, (2):275-302
[117]Bali A, Blanco G, Kennidy C, et al. Excretion ammonium by a nifL-mutant of Azotobacter vinelandi fixing nitrogen. Applied and Environment Microbiology,1992,58:1711-1718
[118]Bashan Y, Holguin G and lifshitz R. Isolation and characterization of plantgrowth-promoting rhizobacteria. In:Glick B R and Thompson J E, eds. Methods in Plant Molecullar Biology and Biotechnology. Boca Raton:CRC Press,1993,331-343
[119]Bashan Y, Levanony H, Mitiku G. Changes in proton efflux of intact wheat roots induced by Azospirillum brasilense Cd. Canadian Journal of Microbiology,1989,35:691-697
[120]Boddey R M, Baldani V L D, Baldani J I, et al. Effect of inoculation of Azospirillum spp. on the nitrogen assimilation of field grown wheat. Plant and Soil,1986,95:109-121
[121]Boddey R M, Urquiaga S, Reis V, et al. Biological nitrogen fixation associate with sugar cane. Plant and Soil,1991,137:111-117
[122]Boddey R M, Urquiaga S, Alves B J R et al. Endophytic nitrogen fixation in sugarcane:present knowledge and future application. Plant and Soil,2003,252(1):139-149
[123]Boddey R M, Dobereiner J. Nitrogen fixation associated with grasses and cereals:Recent progress and perspectives for the future. Fertilizer Research,1995,42(1-3):241-250
[124]Brcmer E., Janzen H H, Gilbertson C. Evidence against associative N2 fixation as a significant N source in long-term wheal-plols. Plant and Soil.1995,175:13-19
[125]Caballoro-Mellado J, Fuente-Ramirez. L E, Rcis V M, et al. Genctic structure of Acelohacter diazolrophicus populations and identifieation of a new genetically distant group. Applied and Environmental Microbiology,1995,61:3008-3013
[126]Canny M J. Apoplaslic wafer and solute movement, New rules for an old space. Annual Review of Plant Physiology and Plant Molecular Biology,1995,46:215-236
[127]Cavalcantc V A, Dobereiner.1. A new acid-tolerant nitrogen-fixing bacterium associated with sugarcane. Plant and Soil,1988,108:23-31
[128]Cheng H H, Bremncr J M, Edwards A P. Variations of nitrogen-15 abundance in soils. Science. 1965,146:1547-1575
1129] Cheng Q. Perspectives in biological nitrogen fixation research. Journal of Integrative Plant Biology,2009,50 (7):786-798
[130]Cocking E C. Bndophytic colonization of plant roots by nitrogen-fixing bacteria. Plant and Soil, 2003,252(1):169-175
[131]Cojho E H, Reis V M, Sehenberg A C G, et al. Interactions of Acelohacter diazotrophicus with an amylolytic yeast in nitrogen-free botch culture. FEMS Microbiology Letters,1993,106:241-346
[132]Costa J M T, Ruschel A P. Seasonal variation in the microbial populations of sugarcane plants. Vose P B, Ruschel A P. Associative N2-fixation. Boca Raton:CRC,1981, (2):109-1181
[133]Da Silva L G D, Reis V M, Dos Rcis F B Jr,et al. Ontogenic variation of diazotrophic bacteria in sugar cane (Saccharum spp.)tissues. Proceedings of the International Symposium on Sustainable Agriculture for The Tropics:the Role of Biological Nitrogen Fixation. Rio de Janeiro, Brazil.1995. 234-235
[134]Danso S K A. Review. Estimation of N2-fixation by isotope dilution:an appraisal of techniques involving 15N enrichment and their application. Soil Biology and Biochemistry,1986,18(3): 243-244
[135]Danso S K A, Hardarson G, Zapata F. Dinitrogen fixation estimates in alfalfa-ryegrass swards using different nitrogen-15 labeling methods. Crop Science,1987,28(1):106-110
[136]Danso SKA, Hardarson G and Zapata F. Misconceptions and practical problems in the use of I5N soil enrichment techniques for estimating N2 fixation. Plant and Soil,1993,152(1):25-52
[137]Dobereiner J. Biological nitrogen fixation in the tropics. Social and Economic Contributions. 1997,29(5-6):771-774
[138]Dobereiner J, Reis V, Lararini A C. New N2 fixing bacteria in association with cereals and sugarance. In:Bothe H, Bruijin F J, Newton W E, eds. Nitrogen Fixation Hundred Years After. Stuttgart:Gustav Fischer, Germany,1988,717-722
[139]Dobereiner J, Ruschel A P. Uma nova especie de Beijerinkia. Revista de Biologia, Rio de Janeiro,1958,1:261-272
[140]Dobereiner J, Day J M. Associative symbioses in tropical grasses:characterization of microorganisms and nitrogen-fixing sites. In:Newton W E, Nyman C J, eds. Nitrogen fixation; proceedings of the 1st International Symposium on Nitrogen Fixation. Pullman:Washington State University,1975,2:518-538
[141]Dobereiner J, Marriel G and Nery M. Ecological distribution of spirillum lipoferum beijerinck. Canadian Journal of Microbiology,1976,22(10):1464-1473
[142]Dobereiner J. History and new perspectives of diazotrophs in association with non-leguminous plants. Symbiosis,1992,13:1-3
[143]Dong Z, Canny M J, McCully M E, et al. A nitrogen-fixing endophyte of sugarcane stems:A new role for the apoplast. Plant Physiology,1994,105:1139-1147
[144]Dong Z, Mccully M E and Canny M J. Does Acetobacter diazotrophicus live and move in the xylem of sugarcane stem? Anatomical and physiological data. Annals of Botany,1997,80: 147-158
[145]Fallik E, Okon Y.Indentification and quantification of IAA and IBA in Azospirillum brasilense-inoculated maize roots. Soil Biology and Biochemistry,1989,21(1):147-153
[146]Fuentes-Ramirez L E, Jimenez-Salgado T, Abarca-Ocampo I R, et al. Acetobacter diazotrophicus, an indoleacetic acid producing bacterium isolated from sugarcane cultivars of Mexico. Plant and Soil,1993,154(2):145-150
[147]Fuentes-Ramirez L E, Caballero-Mellado J, Sepulveda J, et al. Colonization of sugarcane by Acetobacter diazotrophicus is inhibited by high N-fertilization. FEMS Microbiology Ecology, 1999,29(2):117-128
[148]Gholve S G, Sankpal V Y, Jadbav S B. Efficacy of bio-fertilizers under varying levels of nitrogen on nitrogen economy growth and yield of Adsali sugarcane. Indian Sugarcane,1993, (5):323-327
[149]Guo J H, Liu X J, hang Y, et al. Significant acidification in major Chinese croplands. Science, 2010,327:1008-1010
[150]Hegazi N A, Fayez M, Amin G, et al.. Diazotrophs associated with non-legumes grown in sandy soils. In:Malik K A, Mirza M S, Ladha J K. (Eds.), Nitrogen Fixation with Non-Legumes. Kluwer Academic Publishers, Dordrecht,1998,209-222.
[151]Herridge D F, Danso S K A. Enhancing crop legume N2 fixation through selection and breeding. Plant and Soil,1995,174(1-2):51-82
[152]Houlton B Z, Wang Y P, Vitousek P M, et al. A unifying framework for dinitrogen fixation in the terrestrial biosphere. Nature,2008,454:327-331
[153]Hurek T, Reinhold-Hurek B, Van Montagu M, et al. Root colonization and systemic spreading of Azoarcus sp. Strain BH72 in grasses. Journal of Bacteriology,1994,176:1913-1923
[154]Hogberg P, Alexander I J. Roles of root symbioses in African woodland and forest:evidence from 5N abundance and foliar analysis. Journal of Ecology,1995,83:217-224
[155]Ivan R K, Yaotseng T. Biological nitrogen fixation in non-leguminous field crops:Recent advances. Plant and Soil,1992,141(1-2):93-118
[156]James E K. Nitrogen fixation in endophytic and associative symbiosis. Field Crops Research, 2000,65:197-209
[157]James E K, Olivares F L, Andre L M, et al. Further observations on the interaction between sugar cane and Ghnconacetobacter diazolrophicus under laboratory and greenhouse conditions. Journal of Experimental Botany,2001,52 (357):747-760
[158]James E K, Reis V M, Olivarcs F L, et al. Infection of sugarcane by the nitrogen-fixing bacterium Acelobacter diazotrophicus. Experimental Botany,1994,45(275):757-766
[159]Kavadia A, Vayenas D V, Pavlou S, et al. Dynamics of free-living nitrogen-fixing bacterial populations in antagonistic conditions. Ecological modeling,2007,200:243-253
1160] Kennedy L.R., Islam N. The current and potential contribution of asymbiotic nitrogen fixation to nitrogen requirements on farms. Australian Journal of Experimental Agriculture,2001,41: 447-457
1161] Kluepfel I) A. The behaviour and tracking of bacteria in the rhizosphere. Annual Review of Phytopathology,1993,31:441-472
[162]Lajtha K, Marshall J D. Sources of variation in the stable isotopic composition of plants. In: Lajtha K and Mich-ener R H, eds. Stable Isotopes in neology and Environmental Science. Boston: Blackwell Scientific Publications,1994.1-21
[163]Ledgard S F. Nutrition,mois1ure and rhizobial strain influence isotopic fractionation during N.> fixation in Pasture legumes. Soil Biology and Biochemistry,1989.21(1):65-68
[164]Lee T S G. Sugarcane industry in Brazil. In:Li Y R (李样瑞), Solomon S. (Editors-in-Chief). Sustainable Sugarcane and Sugar Production Technology (Proceedings of the International Symposium on Sustainable Sugarcane and Sugar Production Technology, Nanning, Guangxi, China, Nov.29-Dec.O2,2004). Beijing, China:China Agriculture Press,2004,160-165
[165]Li R P, Macrae 1 C. Specific association of diazotrophic acetobacters with sugarcane. Soil Biology and Biochemistry,1991,23(10):999-1002
[166]Liang.J(梁俊), Li Y R (李杨瑞), Liang C X (梁朝旭), et al. The photosynthetic characteristics of different sugarcane varieties without nitrogen fertilization. In:Li Y R (李杨瑞), Solomon S. (Editors-in-Chief). Sustainable Sugarcane and Sugar Production Technology (Proceedings of the International Symposium on Sustainable Sugarcane and Sugar Production Technology, Nanning, Guangxi, China, Nov.29-Dec.O2,2004). Beijing, China:China Agriculture Press,2004,359-362
[167]Lin L, Gao W, Xing Y X, et al.. The actinobacteria Microbacterium sp.16SH accepts pBBR1-based pPROBE vectors, forms biofilms, invades roots, and fixes N2 associated with micropropagated sugarcane plants. Applied Microbiology and Biotechnology,2012,93(3): 1185-1195
[168]Loiret F G, Ortega E, Kleiner D, et al. A putative new endophytic nitrogen-fixing bacterium Pantoea sp. from sugarcane. Journal of Applied Microbiology,2004,97,504-511
[169]Mara Hvistendahl. China's push to add by subtracting fertilizer. Science,2010,327:801
[170]Muthukumarasamy R, Solayappen A R, Narasimban C L. Studies on Azospirillum isolates and yield increase in sugarcane after inoculation with Azospirillum spp. SISSTA Sugar,1990, (1), 19-35
[171]Muthukumarasamy R, Renathi G and Lakshminarasimhan C. Influence of N fertilization on the isolation of Acetobacter diazotrophicu and Herbaspirillum spp. from India sugarcane varieties. Biology and Fertility of Soil,1999,29(2):157-164
[172]Munoz-Rojas J, Caballero-Mellado J. Population dynamics of Gluconacetobader diazotrophicus in sugarcane cultivars and its effect on plant growth. Microbial Ecology,2003,46(4):454-464
[173]Njolama J, Tanaka K, Shimizu T, et al. Infection and colonization of aseptically micropropagated sugarcane seedlings by nitrogen-fixing endophytic bacterium, Herbaspirillum sp. B501gfp1. Biology and Fertility of Soils,2006,43(2):137-143
[174]Patriquin D G, Gracioli L A, Ruschel A P. Nitrogenase activity of sugarcane propagated from stem cuttings in sterile vermiculite. Soil Biology and Biochemistry,1980,12:413-417
[175]Peoples M B, Herridge D F, Ladha J K. Biological nitrogen fixation:An efficient source of nitrogen for sustainable agricultural production? Plant and Soil,1995,174(1-2):3-28
[176]Reis Junior F.B.D., Reis V.M., Urquiaga S., Dobereiner J. Influence of nitrogen fertilization on the population of diazotrophic bacteria Herbaspirillum spp. and Acetobacter diazotrophicus in sugar cane(Saccharum spp.). Plant and Soil,2000,219:153-159
[177]Reis V M, Estrada-de Los Santos P, Tenorio-Salgado S, et al. Burkholderia tropica sp. Nov., a novel nitrogen-fixing, plant-associated bacterium. International Journal of Systematic and Evolutionary Microbiology,2004,54(6):2155-2162
[178]Reis V M, Olivares F L, Dobereiner J. Improved methodology for isolation of Acetobacter diazotrophicus and confirmation of its endophytic habitat. World Journal of Microbiology and Biotechnology,1994,10(4):401-405
[179]Reinhold-Hurek B, Hurek T. Life in grasses:Diazotrophic endophytes. Trends Microbiol.1998, 139-144
[180]Reinhold-Hurek B, Hurek T, Gillis M, et al. Azoarcus gen. nov., Nitrogen-Fixing Proteobacteria Associated with Roots of Kallar Grass(Leptochloa fusca (L.) Kunth), and Description of Two Species, Azoarcus indigens sp. nov. and Azoarcus communis sp. nov. International Journal of Systematic and Evolutionnary Microbiology,1993,43(3):574-584
[181]Rennie R J, Rennie D A, Fried M. Concepts of 15N usage in dinitrogen fixation studies (English). In:Panel Proceedings Series (IAEA), Advisory Group Meeting on Isotopes in Biological Dinitrogen Fixation, Vienna (Austria),21 Nov 1977/FAO, Vienna(Austria). Joint FAO/IAEA Div. of Atomic Energy in Food and Agriculture,1978.107-133
[182]Robert M B, Jose C P, Alexander S R, et al. Use of the 15N natural abundance technique for the quantification of the contribution of N2 fixation to sugar cane and other grasses. Australian Journal of Plant Physiology,2000,28(9):889-895
[183]Ruschel A P, Victoria R L, Salati E, et al. Nitrogen fixation in sugarcane (Saccharum officinarum L.). Blue-green Algae and Asymbiotic Bacteria,1978,26:297-303
[184]Sanginga N, Vanlauwe B, Danso S K A. Management of biological N2 fixation in alley cropping systems:Estimation and contribution to N balance. IMant and Soil,1995.174(1-2):119-141
[185]Schalze E D, Gebauer G,Ziegler H, et al. Estimates of nitrogen fixalion by trees on an aridity gradient in Namibia. Oecologia,1991,88(3):451-155
[186]Shafshak S Δ, E1-Geddawy L H, Allam S Δ H, et al.. Effect of planting densities and nitrogen fertilizer on:1-growth criteria, juice quality and chemical constituents of some sugar cane varieties Pakistan Sugar Journal,2001,16:2-11.
[187]Shearer G, Kohl D H. N2 Fixation in Field Setting:Estimation Based on Natural 15N Abundance. Australian Journal of Plant Physiology,1986,13(6):699-756
[188]Shearer G, Kohl D H, Harper J E. Distribution of N among plant parts of nodulating and non-nodulating isolines of soybean. Plant Physiology,1980,66(1):57-60
[189]Shotaro A D, Sompong M C, Srisuda T A, et al. Evaluation of sustainability of sugarcane production in Thailand based on nitrogen fixation, efficiency of nitrogen fertilizer and flow of organic matters. JIRCAS Working Report,2002,30:61-64
[190]Sprent J I, James E K. N2-fixation by endophytic bacteria:questions of entry and operation. In: Fendric 1, Gallo-de M, Vanderleyden J, Zamaroczy M, eds. Azospirilltnn VI and related organism Berlin:Springer-Verlag,1995,37
[191]Stecnhoudt O, Vanderleyden J. AzospiriUum, a free-living nitrogen-fixing bacterium closely associated with grasses:genetic, bioclicmical and ecological aspects. FEMS Microbiology Review. 2000,24(4):487-506
[192]Stewart W D P. Nitrogen llxalion by free-living micro-organisms. Cambridge (CB):Cambridge University Press,1975.57-70
1193] Segundo U, Katia H S C, Boddey R M, et al. Contribution of nitrogen fixation to sugar cane: nitrogen-15 and nitrogen-balance estimates. Soil Science Society of America Journal,1992,56(1): 105-114
[194]Urquiaga S, Cruz K H S, Boddey R M. Contribution of nitrogen fixation to sugarcane: nitrogen-15 and nitrogen balance estimates. Soil Science Society of America Journal,1992,56(1): 105-114
[195]Urquiaga S, Botteon P B L, Boddey R M. Selection of sugar cane cultivars for associated biological nitrogen fixation using 15N-labelled soil. In:Skinner A. eds. Nitrogen Fixation with Non-Legumes. Kluwer Academic Publishers, Dordrecht, Netherlands,1989.311-319
[196]Veronica M R, Dobereiner J. Effect of high sugar concentration on nitrogenase activity of Acelohacter diazotrophicus. Archives of Microbiology,1998,171:13-18
[197]Wagner G H, Zapata F. Field evaluation of reference crops in the study of nitrogen fixation by legumes using isotope techniques. Agronomy Journal,1981,74(4):607-612
[198]West C P, Wedin W F. Dinitrogen fixation in alfalfa-orchardgrass pastures. Agronomy Journal, 1983,77(1):89-94
[199]Witty J F. Estimating N2-fixation in the field using 15N-labelled fertilizer:some problems and solutions. Soil Biology and Biochemistry,1983,15,631-639
[200]Xing Y X, Yang L T, Huang S L, et al. Identification of a new nitrogen fixing bacterium strain isolated from sugarcane stalk. Sugar Tech,2006,8(1):49-53
[201]Yoneyama T, Yamada N, Kojima H, et al. Variations of natural 15N abundances in leguminous plants and nodule fractions. Plant Cell Physiology,1984,25:1561-1565
[202]Yoneyama T, Muraoka T, Kim T H, et al. The natural 15N abundance of sugarcane and neighbouring plants in Brazil, the Philippines and Miyako (Japan). Plant and Soil,1997,189: 239-244
[203]Zimmer W, Roben K, Bothe H. An alternative explanation for plant growth promotion by bacteria of the genus Azospirillum. Planta,1988,176(3):333-342
[2]陈瑞邦,张必雍,陈业贞,等.旱地甘蔗终止施氮期试验总结.广西农学院学报,1987,(1)：13-15
[3]蔡开地,罗凤来,黄梅卿.春花生施用钙镁硼肥效应函数与分析.植物营养与肥料学报,2002,8(2)：248-250
[4]陈朝勋,席琳乔,姚拓,等.生物固氮测定方法研究进展.草原与草坪,2005,(2)：24-26
[5]陈超君,向立华,宋日云,等.施肥技术对甘蔗产量和品质的影响.广西农业生物科学,2007,26(6)：91-95
[6]陈春,张兴祥,黄可立,等.甘蔗高产栽培模式研究.华南农业大学学报,1991,12(4)：45-51
[7]陈建平,刘伟仲,顾双平.氮肥与化控配合应用对棉花主要经济性状的影响.江苏农业科学1990,(4)：17-19,38
[8]陈建平,顾双平,刘伟仲.氮肥与化控配合应用对棉花产量和品质的影响.棉花学报,19935(2)：49-54
[9]陈明,姚允寅,张希忠.不同形态氮素对15N同位素稀释估测苜蓿固氮的影响.核农学报,1997,18(1)：30-33,40
[10]陈祥龙,李秀章、徐立华,等.小麦后移棉的氮肥、密度效应及器官建成动态研究.棉花学报,1995,7(1)：41-45
[11]陈玉水.种源甘蔗的氮肥需要量和适宜施用期研究.广西蔗糖,2003,33(4)：47-49
[12]戴建军,程岩.应用15N示踪技术对不同品种大豆的三种氮源吸收利用的研究.东北农业大学学报,1999,30(3)：225-229
[13]东秀珠,蔡妙英.常见细菌系统鉴定手册.北京：北京科学出版社,200],128
[14]窦新田.生物固氮.北京：中国农业出版社,1989.17-19
[15]杜丽娟,施书莲,周克瑜,等.应用15N自然丰度法测定固氮植物的固氮量Ⅲ.参比植物的选择.土壤,1996,28(4)：210-212
[16]杜旭华,彭方仁,周贤军.植物的联合固氮机理及研究现状.林业科技情报,2006,38(4)：1-3
[17]方开泰.均匀设计.应用数学学报,1980,3(4)：363-372
[18]樊妙姬,陈丽梅,蒋艳明.甘蔗联合固氮菌对甘蔗叶片养分含量的影响.广西科学,1998,5(4)：311-313
[19]冯海强,潘志强,于翠平,等.利用¨N自然丰度法鉴别有机茶的可行性分析.核农学报,2011,25(2)：0308-0312
[20]龚得名,林彦铨,陈如凯,等.甘蔗不同密度群体的几个光合性状及产量的比较研究.福建农科院学报,1994,9(2)：40-49
[2l]桂意云.不同氮水平对甘蔗生物固氮活性的影响[硕士学位论文].南宁：广西大学,2007
[22]桂意云,刘昔辉,杨荣仲,等.甘蔗不同部位的固氮酶活性检.植物生理学通讯,2007,43(2)：291-294
[23]韩筱渝,李军梅.固氮品种桂引5号种性研究.广西蔗糖,2006,(2)：3-6,11
[24]何道文,孙辉,黄雪菊.利用N-15自然丰度法研究固氮植物生物固氮量.干旱地区农业研究,2004,22(1)：132-137
[25]何天春,岑黄源,覃晓远.甘蔗高产优质不同氮肥量的施用效应.广西蔗糖,2006,45(4)：31-33
[26]黄东风,翁伯琦,罗涛.豆科植物固氮能力的主要测定方法比较.江西农业大学学报,2003,(25)：17-20
[27]黄玉溢,谭宏伟,周柳强,等.几种长效氮肥对甘蔗的效应.广西农学报,2003,(S1)：76-78
[28]蓝立斌,陈超君,米超,等.不同施氮量对甘蔗生理性状、产量和品质的影响.广西农业科学,2010,41(12)：1269-1272
[29]蓝立斌,陈超君,米超,等.不同施氮方式对甘蔗生理生化性状的影响.南方农业学报,2011,42(1)：26-29
[30]李海先,李新民,Seth K A Danso.应用15N稀释法筛选高固氮能力的大豆品种.核农学报,1998,12(5)：299-303
[31]李香真,陈清.”N同位素稀释法测定生物固氮量.核农学通报.1997,(6)：291-293,271
[32]李星洪,李大捷,李薇,等.对从甘蔗根部分离的固氮成团泛菌的研究.北京大学学报(自然科学版),1998,34(6)：741-745
[33]李杨瑞主编.现代甘蔗学.北京：中国农业出版社,2010,85-102,218-263,313-333
[34]李永兴,董越梅,李久蒂.联合固氮菌E57-7泌铵突变株的分离和特性.植物学报,1996,38(7)：563-567
[35]李玉中,Redmann R E,祝廷成,等.羊草草原豆科牧草生物固定量研究.草地学报,2002,10(3)：164-166
[36]李玉潜,谭中文,梁计南.甘蔗对涂层尿素的氮素利用率研究.化肥工业,1994,(6)：24-25
[37]李玉潜,谢九生.甘蔗叶片碳、氮代谢与产量、品质关系研究初探.中国农业科学,1995,28(4)：46-53
[38]梁计南,谭中文,陈真元,等.甘蔗不同施氮方法与产量及糖分的关系.甘蔗,2000,7(4)：1-10
[39]梁俊.不同甘蔗品种的固氮生理特性[硕士学位论文].南宁：广西大学,2004
[40]梁俊,梁朝旭.甘蔗内生固氮的研究进展.广西蔗糖,2006,(2)：25-27
[41]梁俊,李杨瑞,梁朝旭.不同甘蔗品种在无氮肥条件下的光合生理特性.中国农学通报,2005,21(8)：188-207
[42]林昌华,高志红,陈晓远,等.不同施肥水平对粤北坡岗地宿根糖蔗干物质积累和产量的影响.广东农业科学,2010,(6)：63-64
[43]林丽,张新成,李杨瑞,等.甘蔗器官固氮酶活性及其对接种固氮菌的响应.西北植物学报, 2008,28(12)：2472-2477
[44]林敏,尤崇杓.根际联合固氮作用的研究进展. 植物生理通讯,1992,28(5)：323-329
[45]廖明坛,陈克文.供N水平对甘蔗生长和产量的影响.福建农业大学学报,1995,24(2)：195-200
[46]刘光栋,吴文良.高产农田土壤硝态氮淋失与地下水污染动态研究.中国生态农业学报,2003,11(1)：91-93
[47]刘希蝶.旱地甘蔗地膜覆盖、不同施肥次数对比试验初报、甘蔗,1994,1(3)：49-50
[48]刘昔辉.甘蔗内生固氮菌的分离鉴定及不同氮水平对其固氮活性影响[硕士学位论文].广西大学,2008
[49]陆国盈,韩世健,杨培忠,不同施氮量对新台糖16号的产量和品质的影响.广西蔗糖,2001.(4)：3-6
[50]陆国盈,韦晖,蒋明明,等.冰水浸种对不同甘蔗品种生理生化特性的影响.广西农业科学,2010,47(2)：113-116
[51]陆世宏,霍润丰.高旱蔗区地膜覆盖不同施肥次数试验.广西甘蔗,1993,(2)：9-11
[52]陆章流.提高宿根蔗低产低糖的技术措施.广西蔗糖,2005,(1)：3-5
[53]罗霆,欧阳雪庆,杨丽涛,等.1株有固氮能力的甘蔗克雷伯氏菌的分离鉴定及固氮特性.热带作用物报,2010b.31(6)：972-978
[54]罗霆,欧阳雪庆,杨丽涛,等.15N同位素稀释法研究固氮菌接种对甘蔗生物固氮的影响.核农学报,2010a,24(5)：1026-1031
[55]罗霆.甘蔗内生固氮与甘蔗互作的研究[博士学位论文].南宁：广西大学,2010
[56]蒙世欢.广西甘蔗施肥现状、问题及对策.广西农学报,2007,22(5)：37-39
[57]欧阳雪庆,罗霆,杨丽涛,等.甘蔗内生固氮菌液浸种对甘蔗生长前期氮代谢相关酶活性的影响.广西农业科学,2010,41(5)：416-418
[58]覃丽萍,黄思良,李杨瑞.植物内生固氮菌的研究进展.中国农学通报,2005,21(2)：150-152.
[59]山崎肯著.刘步洲,刘宜生,安志信等译.营养液栽培大全.北京：中国农业大学出版社1989：24-25
[60]苏波,韩兴国,黄建辉.15N自然丰度法在生态系统氮索循环研究中的应用.生态学报,1999,19(3)：408-416
[61]苏俊波.甘蔗内生固氮菌的分离与鉴定[硕士学位论文].南宁;广西大学,2006
[62]苏俊波,杨荣仲,桂意云,等.甘蔗体内具有固氮酶活性细菌的分离、鉴定及其相关特性研究.西南农业学报,2007,20(5)：1055-1059
[63]谭裕模,王伦旺,王天算,等.固氮甘蔗品种RB72-454田间肥料敏感试验.广西蔗糖,2002,26(1)：12-15
[64]谭中文,李玉潜,梁计南,等.甘蔗高产高糖高效益综合栽培技术中几种主要措施组配的研究.甘蔗糖业,1994,(2)：11-17
[65]王桂文,李海鹰,范嘉晔.甘蔗内生固氮菌研究.广西农业生物科学,2000,19(1)：68-72
[66]王伦旺,李杨瑞,谭裕模,等.巴西甘蔗品种在广西的种性表现及利用前景.甘蔗糖业,2005a,(5)：1-7
[67]王伦旺,谭裕模,李杨瑞,等.甘蔗品种桂引5号种性研究.中国糖料,2005b,(4)：11-15
[68]王伦旺,李杨瑞,何为中,等.以茎尖组培苗检测甘蔗体内内生固氮菌的固氮活性.植物生理学通讯,2007,43(1)：65-68
[69]王伦旺,李杨瑞,杨荣仲,等.不同甘蔗基因型对低氮胁迫的响应.西南农业学报,2010,23(2)：508-514
[70]王伦旺,杨荣仲,李杨瑞,等.糖能兼用固氮甘蔗品种桂引6号的种性研究.广东农业科学,2011,(12)：20-25
[71]韦东普,白玲玉,华珞,等.用15N同位素稀释法研究牧草的氮素营养.核农学报,2000,14(2)：104-109
[72]韦光旺.早地甘蔗二次施肥试验示范.广西甘蔗,1997,8(3)：17-19
[73]韦剑锋,罗艺,米超,等.施氮次数对甘蔗干物质积累、产量及品质的影响.广东农业科学,2011,(20)：57-59
[74]韦莉萍,李杨瑞,满若军,等.钼对甘蔗氮代谢的影响(英文).广西农业科学,2006,37(6)：648-655
[75]韦莉萍,李杨瑞,杨丽涛.钼对甘蔗体内固氮菌的固氮酶活性的影响.植物营养与肥料学报,2007,13(1)：118-122
[76]文建成,陈学宽,符菊芬,等.质膜透性与丙二醛(MDA)含量的变化.甘蔗,1998,(3)：1-5
[77]吴凯朝,梁俊,罗霆,等.接种内生固氮菌对不同基因型甘蔗农艺特性的效应.广西农业科学,2010,41(6)：528-530
[78]吴凯朝,梁俊,韦莉萍,等.内生固氮菌对甘蔗伸长期光合生理特性的影响.广西植物,2011,31(5)：668-673
[79]席琳乔,张德罡,姚拓.15N同位素稀释法测定燕麦根际固氮菌固氮量的研究.核农学报,2007,21(4)：417-420
[80]邢光熹.同位素自然丰度变异及其在环境污染研究中的应用.核农学通报,1981,(2)：1-6
[81]邢永秀.甘蔗内生固氮细菌的分离鉴定和生长特性[博士学位论文].南宁：广西大学,2006
[82]邢永秀,杨丽涛,李杨瑞.巴西固氮甘蔗品种和广西主栽甘蔗品种氮代谢差异比较.安徽农业科学,2008a,36((21)：9003-9007
[83]邢永秀,杨丽涛,黄思良,等.甘蔗茎内两个内生菌株16S rRNA序列分析及其生长特性.微生物学通报,2008b,35(11)：1732-1737
[84]徐福利,张金水,吕殿青.对苜蓿共生固氮量测定法的比较研究.土壤通报,1993,24(3)：137-139
[85]徐继,李久蒂.生物固氮.生物学通报,1997,32(6)：16-18
[86]徐健,莫小燕,陈远权.等.配方施肥对甘蔗生长及产量的影响.广西热带农业,2010,(4)：24-26.
[87]徐建云,陆耀邦,不同生育期止肥对甘蔗新植和宿根的效应.甘蔗糖业,1991,(2)：11-15
[88]徐林,黄海荣,黄玉溢,等.地下滴灌条件下氮磷钾配施对甘蔗产量及其构成因素的影响.广西农业科学,2010,41(8)：800-803
[89]徐媛,莫建文,吴少维,陈超君.施氮量和氮肥运筹模式对甘蔗前期生长的影响.中国糖料,2011,(1)：20-22,24
[90]许志斌,沈强云,王承莲,等.春小麦高产高效氮磷经济施肥指标研究.宁夏农学院学报,1997,18(1)：73-77
[91]杨荣仲,谭裕模,李杨瑞.甘蔗固氮种质资源筛选研究.2005植物分子育种国际学术研讨会论文集,2005.157-160
[92]杨荣仲,谭裕模,桂意云,等.15N测定甘蔗生物固氮能力研究.安徽农业科学,2008,36(24)：10405-10406
[93]姚拓,蒲小鹏,张德罡,等.高寒地区燕麦根际联合固氮菌研究Ⅲ 固氮菌对燕麦生长的影响及其固氮量测定.草业学报,2004,13(5)：10-15
[94]姚允寅.应用15N进行土壤肥料和植物营养研究的有关数学运算.核农学通报,1988,(8)：186-189
[95]姚允寅,陈明,王志东,等.百里荒牧场饲草15N天然丰度值的测定及其固氮能力初评.中国草地,1992a,(2)：20-24.
[96]姚允寅,陈明,马昌燐,等.几种饲用牧草的δ15N值及其固氮能力初评.同位素,1992b,5(3)：129-134
[97]姚允寅, 陈明,张希忠,等.用15N同位素稀释法评估诱发结瘤春小麦的固氮作用.中国核科技报告,1994,(00)：1-10
[98]尤崇杓,姜涌明,宋鸿遇.生物固氮.北京：科学出版社,1987,228-242
[99]于佰双.应用15N同位素稀释法鉴定大豆新品系的固氮能力.核农学通报,1997,18(1)：34-35
[]00]古新华,将延惠,徐阳春,等.微生物制剂促进植物生长机理的研究进展.植物营养与肥料学报,1999,5(2)：97-105
[101]张朝春,江荣风,张福锁,等.马铃薯氮、磷、钾肥料效应的研究.中国马铃薯,2004,18(6)：326-329
[102]张丽梅.高效联合固氮菌的筛选、鉴定及其生理生化特性和接种效应研究[博士学位论文].杭州：浙江大学,2002
[104]张丽梅,方萍,朱日清.禾本科植物联合固氮研究及其应用现状展望.应用生态学报,2004,15(9)：1650-1654
[105]赵淑清,田春杰,何兴元.固氮植物的菌根研究.应用生态学报,2000,11(2)：306-310
[106]周汉仁.甘蔗不同施氮期对产量和蔗糖份的影响.甘蔗糖业,1984,(9)：26-28
[107]周克瑜,施书莲,杜丽娟,等.豆科固氮植物植株茎叶、根和根瘤的δ15N值变异.核农学报,1998,12(2)：105-111
[108]周修冲,刘国坚,姚建武,等.旱地甘蔗提高氮肥利用率的施肥技术研究.土壤肥料,2000,(6)：42-43
[109]周正邦,龚德勇,易代勇,等.不同施氮水平对固氮甘蔗品种RB72-454的肥效响应.贵州农业科学,2006,34(1)：52-55
[110]周正邦,龚德勇,易代勇,等.4个甘蔗新品种田间氮肥敏感性试验.贵州农业科学,2004,32(5)：69-70
[111]周正邦,易代勇,龚德勇,等.氮肥对高糖甘蔗品种的增效作用.贵州农业科学,2009,37(8)：65-66
[112]褚贵新,沈其荣,张娟,等.用15N富积标记和稀释法研究旱作水稻/花生间作系统中氮素固定和转移.植物营养与施肥学报,2003,9(4)：385-389
[113]Ando S, Meunchang S, Thippayarugs S, et al. Role of nitrogen fixation for sustainable sugarcane production. In:Li Y R, Solomon S, eds. Sustainable Sugarcane and Sugar Production Technology. Beijing:China Agriculture Press,2004,156-159
[114]Andre M. Atmospheric nitrogen is a reliable standard for natural 15N abundance measurements. Nature,1983,303(23):685-687
[115]Asis Jr C A, Kubota M, Ohta H, et al. Isolation and partial characterization of endophytic diazotrophs associated with Japanese sugarcane cultivar. Soil Science and Plant Nutrition,2000, 46(3):759-765
[116]Balandreau J. Non-symbiotic nitrogen fixation in rhizosphere of rice maize and different. Nitrogen Fixation,1980, (2):275-302
[117]Bali A, Blanco G, Kennidy C, et al. Excretion ammonium by a nifL-mutant of Azotobacter vinelandi fixing nitrogen. Applied and Environment Microbiology,1992,58:1711-1718
[118]Bashan Y, Holguin G and lifshitz R. Isolation and characterization of plantgrowth-promoting rhizobacteria. In:Glick B R and Thompson J E, eds. Methods in Plant Molecullar Biology and Biotechnology. Boca Raton:CRC Press,1993,331-343
[119]Bashan Y, Levanony H, Mitiku G. Changes in proton efflux of intact wheat roots induced by Azospirillum brasilense Cd. Canadian Journal of Microbiology,1989,35:691-697
[120]Boddey R M, Baldani V L D, Baldani J I, et al. Effect of inoculation of Azospirillum spp. on the nitrogen assimilation of field grown wheat. Plant and Soil,1986,95:109-121
[121]Boddey R M, Urquiaga S, Reis V, et al. Biological nitrogen fixation associate with sugar cane. Plant and Soil,1991,137:111-117
[122]Boddey R M, Urquiaga S, Alves B J R et al. Endophytic nitrogen fixation in sugarcane:present knowledge and future application. Plant and Soil,2003,252(1):139-149
[123]Boddey R M, Dobereiner J. Nitrogen fixation associated with grasses and cereals:Recent progress and perspectives for the future. Fertilizer Research,1995,42(1-3):241-250
[124]Brcmer E., Janzen H H, Gilbertson C. Evidence against associative N2 fixation as a significant N source in long-term wheal-plols. Plant and Soil.1995,175:13-19
[125]Caballoro-Mellado J, Fuente-Ramirez. L E, Rcis V M, et al. Genctic structure of Acelohacter diazolrophicus populations and identifieation of a new genetically distant group. Applied and Environmental Microbiology,1995,61:3008-3013
[126]Canny M J. Apoplaslic wafer and solute movement, New rules for an old space. Annual Review of Plant Physiology and Plant Molecular Biology,1995,46:215-236
[127]Cavalcantc V A, Dobereiner.1. A new acid-tolerant nitrogen-fixing bacterium associated with sugarcane. Plant and Soil,1988,108:23-31
[128]Cheng H H, Bremncr J M, Edwards A P. Variations of nitrogen-15 abundance in soils. Science. 1965,146:1547-1575
1129] Cheng Q. Perspectives in biological nitrogen fixation research. Journal of Integrative Plant Biology,2009,50 (7):786-798
[130]Cocking E C. Bndophytic colonization of plant roots by nitrogen-fixing bacteria. Plant and Soil, 2003,252(1):169-175
[131]Cojho E H, Reis V M, Sehenberg A C G, et al. Interactions of Acelohacter diazotrophicus with an amylolytic yeast in nitrogen-free botch culture. FEMS Microbiology Letters,1993,106:241-346
[132]Costa J M T, Ruschel A P. Seasonal variation in the microbial populations of sugarcane plants. Vose P B, Ruschel A P. Associative N2-fixation. Boca Raton:CRC,1981, (2):109-1181
[133]Da Silva L G D, Reis V M, Dos Rcis F B Jr,et al. Ontogenic variation of diazotrophic bacteria in sugar cane (Saccharum spp.)tissues. Proceedings of the International Symposium on Sustainable Agriculture for The Tropics:the Role of Biological Nitrogen Fixation. Rio de Janeiro, Brazil.1995. 234-235
[134]Danso S K A. Review. Estimation of N2-fixation by isotope dilution:an appraisal of techniques involving 15N enrichment and their application. Soil Biology and Biochemistry,1986,18(3): 243-244
[135]Danso S K A, Hardarson G, Zapata F. Dinitrogen fixation estimates in alfalfa-ryegrass swards using different nitrogen-15 labeling methods. Crop Science,1987,28(1):106-110
[136]Danso SKA, Hardarson G and Zapata F. Misconceptions and practical problems in the use of I5N soil enrichment techniques for estimating N2 fixation. Plant and Soil,1993,152(1):25-52
[137]Dobereiner J. Biological nitrogen fixation in the tropics. Social and Economic Contributions. 1997,29(5-6):771-774
[138]Dobereiner J, Reis V, Lararini A C. New N2 fixing bacteria in association with cereals and sugarance. In:Bothe H, Bruijin F J, Newton W E, eds. Nitrogen Fixation Hundred Years After. Stuttgart:Gustav Fischer, Germany,1988,717-722
[139]Dobereiner J, Ruschel A P. Uma nova especie de Beijerinkia. Revista de Biologia, Rio de Janeiro,1958,1:261-272
[140]Dobereiner J, Day J M. Associative symbioses in tropical grasses:characterization of microorganisms and nitrogen-fixing sites. In:Newton W E, Nyman C J, eds. Nitrogen fixation; proceedings of the 1st International Symposium on Nitrogen Fixation. Pullman:Washington State University,1975,2:518-538
[141]Dobereiner J, Marriel G and Nery M. Ecological distribution of spirillum lipoferum beijerinck. Canadian Journal of Microbiology,1976,22(10):1464-1473
[142]Dobereiner J. History and new perspectives of diazotrophs in association with non-leguminous plants. Symbiosis,1992,13:1-3
[143]Dong Z, Canny M J, McCully M E, et al. A nitrogen-fixing endophyte of sugarcane stems:A new role for the apoplast. Plant Physiology,1994,105:1139-1147
[144]Dong Z, Mccully M E and Canny M J. Does Acetobacter diazotrophicus live and move in the xylem of sugarcane stem? Anatomical and physiological data. Annals of Botany,1997,80: 147-158
[145]Fallik E, Okon Y.Indentification and quantification of IAA and IBA in Azospirillum brasilense-inoculated maize roots. Soil Biology and Biochemistry,1989,21(1):147-153
[146]Fuentes-Ramirez L E, Jimenez-Salgado T, Abarca-Ocampo I R, et al. Acetobacter diazotrophicus, an indoleacetic acid producing bacterium isolated from sugarcane cultivars of Mexico. Plant and Soil,1993,154(2):145-150
[147]Fuentes-Ramirez L E, Caballero-Mellado J, Sepulveda J, et al. Colonization of sugarcane by Acetobacter diazotrophicus is inhibited by high N-fertilization. FEMS Microbiology Ecology, 1999,29(2):117-128
[148]Gholve S G, Sankpal V Y, Jadbav S B. Efficacy of bio-fertilizers under varying levels of nitrogen on nitrogen economy growth and yield of Adsali sugarcane. Indian Sugarcane,1993, (5):323-327
[149]Guo J H, Liu X J, hang Y, et al. Significant acidification in major Chinese croplands. Science, 2010,327:1008-1010
[150]Hegazi N A, Fayez M, Amin G, et al.. Diazotrophs associated with non-legumes grown in sandy soils. In:Malik K A, Mirza M S, Ladha J K. (Eds.), Nitrogen Fixation with Non-Legumes. Kluwer Academic Publishers, Dordrecht,1998,209-222.
[151]Herridge D F, Danso S K A. Enhancing crop legume N2 fixation through selection and breeding. Plant and Soil,1995,174(1-2):51-82
[152]Houlton B Z, Wang Y P, Vitousek P M, et al. A unifying framework for dinitrogen fixation in the terrestrial biosphere. Nature,2008,454:327-331
[153]Hurek T, Reinhold-Hurek B, Van Montagu M, et al. Root colonization and systemic spreading of Azoarcus sp. Strain BH72 in grasses. Journal of Bacteriology,1994,176:1913-1923
[154]Hogberg P, Alexander I J. Roles of root symbioses in African woodland and forest:evidence from 5N abundance and foliar analysis. Journal of Ecology,1995,83:217-224
[155]Ivan R K, Yaotseng T. Biological nitrogen fixation in non-leguminous field crops:Recent advances. Plant and Soil,1992,141(1-2):93-118
[156]James E K. Nitrogen fixation in endophytic and associative symbiosis. Field Crops Research, 2000,65:197-209
[157]James E K, Olivares F L, Andre L M, et al. Further observations on the interaction between sugar cane and Ghnconacetobacter diazolrophicus under laboratory and greenhouse conditions. Journal of Experimental Botany,2001,52 (357):747-760
[158]James E K, Reis V M, Olivarcs F L, et al. Infection of sugarcane by the nitrogen-fixing bacterium Acelobacter diazotrophicus. Experimental Botany,1994,45(275):757-766
[159]Kavadia A, Vayenas D V, Pavlou S, et al. Dynamics of free-living nitrogen-fixing bacterial populations in antagonistic conditions. Ecological modeling,2007,200:243-253
1160] Kennedy L.R., Islam N. The current and potential contribution of asymbiotic nitrogen fixation to nitrogen requirements on farms. Australian Journal of Experimental Agriculture,2001,41: 447-457
1161] Kluepfel I) A. The behaviour and tracking of bacteria in the rhizosphere. Annual Review of Phytopathology,1993,31:441-472
[162]Lajtha K, Marshall J D. Sources of variation in the stable isotopic composition of plants. In: Lajtha K and Mich-ener R H, eds. Stable Isotopes in neology and Environmental Science. Boston: Blackwell Scientific Publications,1994.1-21
[163]Ledgard S F. Nutrition,mois1ure and rhizobial strain influence isotopic fractionation during N.> fixation in Pasture legumes. Soil Biology and Biochemistry,1989.21(1):65-68
[164]Lee T S G. Sugarcane industry in Brazil. In:Li Y R (李样瑞), Solomon S. (Editors-in-Chief). Sustainable Sugarcane and Sugar Production Technology (Proceedings of the International Symposium on Sustainable Sugarcane and Sugar Production Technology, Nanning, Guangxi, China, Nov.29-Dec.O2,2004). Beijing, China:China Agriculture Press,2004,160-165
[165]Li R P, Macrae 1 C. Specific association of diazotrophic acetobacters with sugarcane. Soil Biology and Biochemistry,1991,23(10):999-1002
[166]Liang.J(梁俊), Li Y R (李杨瑞), Liang C X (梁朝旭), et al. The photosynthetic characteristics of different sugarcane varieties without nitrogen fertilization. In:Li Y R (李杨瑞), Solomon S. (Editors-in-Chief). Sustainable Sugarcane and Sugar Production Technology (Proceedings of the International Symposium on Sustainable Sugarcane and Sugar Production Technology, Nanning, Guangxi, China, Nov.29-Dec.O2,2004). Beijing, China:China Agriculture Press,2004,359-362
[167]Lin L, Gao W, Xing Y X, et al.. The actinobacteria Microbacterium sp.16SH accepts pBBR1-based pPROBE vectors, forms biofilms, invades roots, and fixes N2 associated with micropropagated sugarcane plants. Applied Microbiology and Biotechnology,2012,93(3): 1185-1195
[168]Loiret F G, Ortega E, Kleiner D, et al. A putative new endophytic nitrogen-fixing bacterium Pantoea sp. from sugarcane. Journal of Applied Microbiology,2004,97,504-511
[169]Mara Hvistendahl. China's push to add by subtracting fertilizer. Science,2010,327:801
[170]Muthukumarasamy R, Solayappen A R, Narasimban C L. Studies on Azospirillum isolates and yield increase in sugarcane after inoculation with Azospirillum spp. SISSTA Sugar,1990, (1), 19-35
[171]Muthukumarasamy R, Renathi G and Lakshminarasimhan C. Influence of N fertilization on the isolation of Acetobacter diazotrophicu and Herbaspirillum spp. from India sugarcane varieties. Biology and Fertility of Soil,1999,29(2):157-164
[172]Munoz-Rojas J, Caballero-Mellado J. Population dynamics of Gluconacetobader diazotrophicus in sugarcane cultivars and its effect on plant growth. Microbial Ecology,2003,46(4):454-464
[173]Njolama J, Tanaka K, Shimizu T, et al. Infection and colonization of aseptically micropropagated sugarcane seedlings by nitrogen-fixing endophytic bacterium, Herbaspirillum sp. B501gfp1. Biology and Fertility of Soils,2006,43(2):137-143
[174]Patriquin D G, Gracioli L A, Ruschel A P. Nitrogenase activity of sugarcane propagated from stem cuttings in sterile vermiculite. Soil Biology and Biochemistry,1980,12:413-417
[175]Peoples M B, Herridge D F, Ladha J K. Biological nitrogen fixation:An efficient source of nitrogen for sustainable agricultural production? Plant and Soil,1995,174(1-2):3-28
[176]Reis Junior F.B.D., Reis V.M., Urquiaga S., Dobereiner J. Influence of nitrogen fertilization on the population of diazotrophic bacteria Herbaspirillum spp. and Acetobacter diazotrophicus in sugar cane(Saccharum spp.). Plant and Soil,2000,219:153-159
[177]Reis V M, Estrada-de Los Santos P, Tenorio-Salgado S, et al. Burkholderia tropica sp. Nov., a novel nitrogen-fixing, plant-associated bacterium. International Journal of Systematic and Evolutionary Microbiology,2004,54(6):2155-2162
[178]Reis V M, Olivares F L, Dobereiner J. Improved methodology for isolation of Acetobacter diazotrophicus and confirmation of its endophytic habitat. World Journal of Microbiology and Biotechnology,1994,10(4):401-405
[179]Reinhold-Hurek B, Hurek T. Life in grasses:Diazotrophic endophytes. Trends Microbiol.1998, 139-144
[180]Reinhold-Hurek B, Hurek T, Gillis M, et al. Azoarcus gen. nov., Nitrogen-Fixing Proteobacteria Associated with Roots of Kallar Grass(Leptochloa fusca (L.) Kunth), and Description of Two Species, Azoarcus indigens sp. nov. and Azoarcus communis sp. nov. International Journal of Systematic and Evolutionnary Microbiology,1993,43(3):574-584
[181]Rennie R J, Rennie D A, Fried M. Concepts of 15N usage in dinitrogen fixation studies (English). In:Panel Proceedings Series (IAEA), Advisory Group Meeting on Isotopes in Biological Dinitrogen Fixation, Vienna (Austria),21 Nov 1977/FAO, Vienna(Austria). Joint FAO/IAEA Div. of Atomic Energy in Food and Agriculture,1978.107-133
[182]Robert M B, Jose C P, Alexander S R, et al. Use of the 15N natural abundance technique for the quantification of the contribution of N2 fixation to sugar cane and other grasses. Australian Journal of Plant Physiology,2000,28(9):889-895
[183]Ruschel A P, Victoria R L, Salati E, et al. Nitrogen fixation in sugarcane (Saccharum officinarum L.). Blue-green Algae and Asymbiotic Bacteria,1978,26:297-303
[184]Sanginga N, Vanlauwe B, Danso S K A. Management of biological N2 fixation in alley cropping systems:Estimation and contribution to N balance. IMant and Soil,1995.174(1-2):119-141
[185]Schalze E D, Gebauer G,Ziegler H, et al. Estimates of nitrogen fixalion by trees on an aridity gradient in Namibia. Oecologia,1991,88(3):451-155
[186]Shafshak S Δ, E1-Geddawy L H, Allam S Δ H, et al.. Effect of planting densities and nitrogen fertilizer on:1-growth criteria, juice quality and chemical constituents of some sugar cane varieties Pakistan Sugar Journal,2001,16:2-11.
[187]Shearer G, Kohl D H. N2 Fixation in Field Setting:Estimation Based on Natural 15N Abundance. Australian Journal of Plant Physiology,1986,13(6):699-756
[188]Shearer G, Kohl D H, Harper J E. Distribution of N among plant parts of nodulating and non-nodulating isolines of soybean. Plant Physiology,1980,66(1):57-60
[189]Shotaro A D, Sompong M C, Srisuda T A, et al. Evaluation of sustainability of sugarcane production in Thailand based on nitrogen fixation, efficiency of nitrogen fertilizer and flow of organic matters. JIRCAS Working Report,2002,30:61-64
[190]Sprent J I, James E K. N2-fixation by endophytic bacteria:questions of entry and operation. In: Fendric 1, Gallo-de M, Vanderleyden J, Zamaroczy M, eds. Azospirilltnn VI and related organism Berlin:Springer-Verlag,1995,37
[191]Stecnhoudt O, Vanderleyden J. AzospiriUum, a free-living nitrogen-fixing bacterium closely associated with grasses:genetic, bioclicmical and ecological aspects. FEMS Microbiology Review. 2000,24(4):487-506
[192]Stewart W D P. Nitrogen llxalion by free-living micro-organisms. Cambridge (CB):Cambridge University Press,1975.57-70
1193] Segundo U, Katia H S C, Boddey R M, et al. Contribution of nitrogen fixation to sugar cane: nitrogen-15 and nitrogen-balance estimates. Soil Science Society of America Journal,1992,56(1): 105-114
[194]Urquiaga S, Cruz K H S, Boddey R M. Contribution of nitrogen fixation to sugarcane: nitrogen-15 and nitrogen balance estimates. Soil Science Society of America Journal,1992,56(1): 105-114
[195]Urquiaga S, Botteon P B L, Boddey R M. Selection of sugar cane cultivars for associated biological nitrogen fixation using 15N-labelled soil. In:Skinner A. eds. Nitrogen Fixation with Non-Legumes. Kluwer Academic Publishers, Dordrecht, Netherlands,1989.311-319
[196]Veronica M R, Dobereiner J. Effect of high sugar concentration on nitrogenase activity of Acelohacter diazotrophicus. Archives of Microbiology,1998,171:13-18
[197]Wagner G H, Zapata F. Field evaluation of reference crops in the study of nitrogen fixation by legumes using isotope techniques. Agronomy Journal,1981,74(4):607-612
[198]West C P, Wedin W F. Dinitrogen fixation in alfalfa-orchardgrass pastures. Agronomy Journal, 1983,77(1):89-94
[199]Witty J F. Estimating N2-fixation in the field using 15N-labelled fertilizer:some problems and solutions. Soil Biology and Biochemistry,1983,15,631-639
[200]Xing Y X, Yang L T, Huang S L, et al. Identification of a new nitrogen fixing bacterium strain isolated from sugarcane stalk. Sugar Tech,2006,8(1):49-53
[201]Yoneyama T, Yamada N, Kojima H, et al. Variations of natural 15N abundances in leguminous plants and nodule fractions. Plant Cell Physiology,1984,25:1561-1565
[202]Yoneyama T, Muraoka T, Kim T H, et al. The natural 15N abundance of sugarcane and neighbouring plants in Brazil, the Philippines and Miyako (Japan). Plant and Soil,1997,189: 239-244
[203]Zimmer W, Roben K, Bothe H. An alternative explanation for plant growth promotion by bacteria of the genus Azospirillum. Planta,1988,176(3):333-342
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |