不同时间电针干预对实验性脑出血大鼠炎性免疫反应的研究
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摘要
目的
观察脑出血后血肿周围脑组织炎症因子的动态变化以及不同时间点电针治疗对其的影响,全面了解多种炎症因子在ICH后的作用机制,探讨电针治疗急性脑出血后炎性免疫反应的可能的干预机制。
方法
体重为200±20g的健康成年Wistar雄性大鼠,随机分为4大组：正常组、假手术组、模型对照组、电针治疗组,模型对照组分为5个时间点,即分为造模后6h、24h、48h、72h、8天五个小组；电针治疗组,即将电针治疗组分为6h、24h、48h、72h四个小组。正常组不做任何处理,假手术组造模时用生理盐水代替胶原酶Ⅶ进行造模,模型对照组和电针治疗组采用0.5u胶原酶Ⅶ注射大鼠尾壳核进行造模,确定造模成功后电针治疗组开始做治疗,分别于造模后相应的时间点开始针刺,取“水沟”、“百会”、“足三里”(双侧),接G6805—2型电针仪,疏密波,强度1mA,每次留针30min,每日电针一次,连续电针治疗5次。模型对照组不做治疗,在造模后的6h、24h、48h、72h、8天相应的时间点取材,电针治疗组分别在造模后相应时间开始做治疗,分别连续治疗5天后取材,进行相关指标的检测。应用干湿法检测血肿周围脑组织的含水量,用HE染色观察血肿周围组织形态学的变化,分别用免疫组化和western-blot检测ICAM、MMP-2、MMP-9、TIMP-1的表达。
结果
(1)HE染色结果：正常组和假手术组可见丰富的神经细胞,排列整齐,核仁清晰。6h模型对照组大鼠尾状核血肿区可见大片出血灶及点状出血,红细胞浸润,神经细胞坏死,髓质可见部分片状间质轻度水肿；24h可见大量的空泡,中性粒细胞和淋巴细胞浸润现象,炎症反应明显；48h血肿周围脑组织水肿严重,可见大量小胶质细胞浸润和明显的炎性细胞浸润；72h脑组织内脑组织水肿和细胞间隙空化最为明显,周围可见较多和炎性细胞浸润；8天模型对照组血肿周围轻度水肿；6h电针治疗组脑组织内可见点状出血,细胞间质仍有轻度水肿,可见部分中性粒细胞浸润和胶质细胞；与6h模型对照组比较相似。24h电针治疗组脑组织内可见水肿明显减轻,坏死灶明显缩小、极少量出血,有一定的中性粒细胞浸润。48h电针治疗组,水肿明显减轻,可见少量见中性粒细胞浸润,72h电针治疗组可见水肿基本消失,与8天模型对照组比较有明显的好转。电针治疗能明显减轻炎症反应,减少炎症细胞的浸润。
(2)脑出血大鼠各时间点脑组织含水量的结果：6h模型对照组脑含水量有一定程度的增加,24h组脑含水量明显增高,72h时含水量达到高峰,在8天时逐渐下降,模型对照组与正常组和假手术组比较有显著性差异(P<0.01),而相应时间点的电针治疗组与模型对照组比较：6h电针治疗组与6h模型对照组比较,差异无统计学意义(P>0.05),与8天模型对照组比较,差异无统计学意义(P>0.05)；其余相应时间点的电针治疗组与模型对照组比较有显著性的差异(P<0.01),与8天模型对照组比较有显著性的统计学意义(P<0.01)。除了6h电针治疗组,其余时间点进行电针治疗能明显减轻脑出血大鼠脑组织的含水量,减轻脑水肿。
(3) ICAM-1免疫组化和免疫印迹结果显示：模型对照组的各个时间点血肿周围组织均有不同程度ICAM-1阳性反应的内皮细胞和神经细胞,脑出血后6h周围血管和神经细胞均有ICAM-1的表达,24h、48h ICAM-1表达持续增加,72h达高峰,至8d仍有较高水平的表达。各电针治疗组与对应时间点的模型对照组ICAM-1的表达比较有显著性的差异(P<0.01),6h电针治疗组与6h模型对照组比较差异有统计学意义(P<0.01),但是作用却是增加了ICAM-1的表达,与8天组比较无统计学意义(P>0.05)；其余电针治疗组与对应时间点的模型对照组ICAM-1的表达比较有显著性的差异(P<0.01),与8天组比较有显著性的差异(P<0.05)。除了6h电针治疗组,其余时间点进行电针治疗能降低脑出血后大鼠脑组织中ICAM-1的表达。
(4)脑组织中MMP-9.TIMP-1表达结果显示：正常组未见明显的MMP-9、 TIMP-1的表达,模型对照组6h可见MMP-9明显表达,24h表达逐渐增强,在48h组达到最高峰,在8天时有少量水平的低表达；模型对照组TIMP-1的表达与MMP-9各组的表达相似。经过电针治疗后,除6h电针治疗组外电针治疗组各时间点MMP-9的表达较模型对照组的各时间点比较都有明显的减少,而TIMP-1表达较模型对照组各时间点比较都有明显的增加。统计学的比较：6h电针治疗组分别与6h模型对照组和8天模型对照组比较都无统计学意义(P>0.05),而其余各电针治疗组与对应时间点的模型对照组的MMP-9、TIMP-1的表达比较,存在着显著性的差异(P<0.01),24h、48h、72h组电针治疗组与8天模型对照组比较都有显著性的差异(P<0.05),电针能降低脑出血大鼠血肿周围MMP-9和升高TIMP-1的表达。
(5)脑组织中MMP-2表达结果显示：正常组与假手术组MMP-2的表达无明显差别(P>0.05),各模型对照组较假手术组和正常组都有显著性的差异(P<0.01)；6h模型对照组可见MMP-2的明显表达,24h持续增高至最高峰,48h-72h逐渐减少,但仍明显高于正常组,8天还有高水平的表达；经电针治疗后,除6h电针治疗组外,其余电针治疗组各时间点MMP2的表达明显的减少,6h电针治疗组分别与6h模型对照组和8天模型对照组比较,MMP-2的表达均无统计学意义(P>0.05)；其余电针治疗组与对应时间点的模型对照组MMP-2的表达比较有显著性的差异(P<0.01),与8天模型对照组比较有显著性的差异(P<0.01)。电针能够降低脑出血大鼠血肿周围组织MMP-2的表达。
结论
本研究表明在脑出血后的急性期选取百会、水沟、足三里进行电针治疗,除6h电针治疗组外,其余各时间点电针治疗均能明显降低脑出血大鼠血肿周围组织ICAM-1、MMP-9、MMP-2的表达,升高TIMP-1的表达,从而发挥抗脑出血后炎症反应的作用,是针灸具有多靶点、多通道作用于炎症细胞和神经元的结果。
Objective
By invstergation the dynamic changes of inflammatory factors surrounding hematoma after Cerebral hemorrhage and its impact of electro-acupuncture therapy at different time points, comprehend the mechanism of inflammatory factors atfter ICH, study the possible intervention mechanism about inflammatory immune response of the electroacupuncture treatment after acute cerebral hemorrhage.
Methods
Choosing120male Wistar rats were randomly divided into normal group, sham-operated group, model group and EA group, normal group, sham-operated group, with18rats each team, the model groups were divided into6h,24h,48h,72h,8days5points, with18rats each team, EA groups were divided into, with18rats each team. the model groups and EA groups Use the0.5u collagenase VII injected rats caudate nucleus to make the modelof experimental, we applied the baihui、shuigou、zusanli (both sides) for the EA group, In the corresponding time points after modeling6h,24h,48h,72h,the EA begined to Scalp-acupuncture therapy (Continuous-wave frequency of120beats/min, intensity of1mA, the treatment time30min, Once a day, five days in a row.) there wasn't any treatment of other groups.In the corresponding time points rats were killed to detect of relevant indicators, Including to measure the brain water content by dry-wet weigh method and observe the morphological changes of perihematomaby He staining, The others were to measure the protein expressions of I CAM、MMP-2、MMP-9、 TIMP-1by Immunohistochemical and Western blot
Result
(1)The results of HE staining indicate:There were no significant swelling of normal group and sham operation group of nerve cells, arranging in neat rows, structural integrity and a clear nucleolus. The model group6h rat caudate nucleus hematoma can be seen large areas of hemorrhage and spotting red blood cell infiltration, necrosis of nerve cells, the medulla visible interstitial part of the sheet with mild edema;24h can be seen a large number of vacuoles, neutrophil and lymphocyte infiltration, inflammation obvious; serious48h hematoma surrounding brain edema, seen a large number of microglia infiltration and inflammatory cell infiltration;72h brain tissue within the brain tissueedema and cell gap cavitation is most evident around can be seen more and inflammatory cell infiltration; eight days of the model group with mild edema around the hematoma;6h EA group of brain tissue seen in spotting, stromal cells, any mild edema, visible part of the neutrophil infiltration and glial cells; with the model group6h is quite similar. Visible edema within the brain tissue of24h EA group significantly reduced necrosis significantly reduced, very small amount of bleeding, certain degree of neutrophil infiltration. 48h EA group, the edema was reduced, visible see a small amount of neutrophil infiltration,72h EA group visible edema disappeared, with the model group, a marked improvement in eight days. EA treatment can significantly reduce inflammation, reduce the infiltration of inflammatory cells.
(2) The water content of brain of Cerebral hemorrhage rats at each time point tissue indicate:6h model group, brain water content increase,24h, the bleeding side of the brain water content was significantly increased, peaked at72h,24-72h to maintain a high level and then decreased, a significant difference (P<0.01) compared with normalthe corresponding point in time the EA group compared with model group, EA group6h6h model group no significant difference between the rest of the EA of the corresponding point in time with the model group and there was significant sex difference (P<0.01), compared with eight days of model group was statistically significant (P<0.05),6h,24h,72h, the EA group after electro-acupuncture treatment can significantly reduce the water content of brain tissue among the groups no significant sexdifferences.
(3) ICAM-1immunohistochemistry and Western blot results show: Model groups perihematoma have varying degrees of ICAM-1positive endothelial cells and neurons,6hours after intracerebral hemorrhage around blood vessels and nerve cells are the expression of ICAM-1,24h,48h, ICAM-1expression continued to increase,72hreached the peak at a higher level of expression in the8d any. EA group compared with the corresponding time point of the model group, ICAM-1expression, a significant sex difference (P<0.01),6h group of eight days of the EA group was not statistically significant (P>0.05), EA24h group,48h,72h group and8days group significantly with sex differences (P<0.05), while between EA24h group,48h,72h group group comparison no obvious significant difference (P>0.05), electro-acupuncture can reduce ICAM1expression
(4) MMP-9、TIMP-limmunohistochemistry and Western blot results show:Normal group, no significant MMP-9, TIMP-1expression, the model control group6h visible significant expression of MMP-9,24h expression gradually increased and reached a peak at48h group, a small amount of low level of expression at day8; model controlgroup of TIMP-1expression and MMP-9expression of each group. After electro-acupuncture treatment in addition to the6h group EA, the EA treatment group time points of MMP-9expression compared with the model control group at each time point comparison has significantly reduced; In addition to the6h group of electro-acupuncture, electro-acupuncture treatment group a TIMP-1expression, the point in time relatively significant increase compared with the model control group at each time point. Statistical comparison:6h group of electro-acupuncture treatment control group6h group and8days respectively, and the model comparisons are not statistically significant (P>0.05), while the rest of the EA group and the corresponding time point of the model control group, MMP-9of TIMP-1of expression exist with significant differences (P<0.01), EA24h group,48h,72h group and8days group has significantly with the nature of the differences (<0.05), electro-acupuncture can reduce the cerebral hemorrhage around the hematoma in rats, MMP-9and increased TIMP-1expression.
(5) MMP2immunohistochemistry and Western blot results show: Normal group and sham operation group, the expression of MMP-2was no significant difference (P>0.05), each model group compared with the sham group and normal group has a significant sex difference (P<0.01); model control group6h visible MMP-2a significant expression to24h continued to increase to the highest peak,48h-72h gradually reduced, but still significantly higher than the normal group, eight days, there is a high level of expression; after electro-acupuncture therapy, except for electro-acupuncture treatment group6h, and the remaining EAsignificantly reduce the treatment group at each time point MMP2in6h group of electro-acupuncture treatment6h group and8days compared with the model control, MMP-2expression were not statistically significant (P>0.05); the rest of the EA treatment groupcompared with the model control group the corresponding time points of MMP-2expression of differences (P<0.01), compared with eight days of model control group, significant sex differences (P<0.01). EA can reduce intracerebral hemorrhage in rats hematoma surrounding tissue expression of MMP-2.
Conclution:The study shows that after the acute phase of cerebral hemorrhage, electro-acupuncture treatment has significantly reduce ICAM-1, MMP-9, MMP-2expression and increased TIMP-1expression,So that induced anti-inflammatory response after anti-cerebral hemorrhage, which is the result of acupuncture's multi-target, multi-channel role
观察脑出血后血肿周围脑组织炎症因子的动态变化以及不同时间点电针治疗对其的影响,全面了解多种炎症因子在ICH后的作用机制,探讨电针治疗急性脑出血后炎性免疫反应的可能的干预机制。
方法
体重为200±20g的健康成年Wistar雄性大鼠,随机分为4大组：正常组、假手术组、模型对照组、电针治疗组,模型对照组分为5个时间点,即分为造模后6h、24h、48h、72h、8天五个小组；电针治疗组,即将电针治疗组分为6h、24h、48h、72h四个小组。正常组不做任何处理,假手术组造模时用生理盐水代替胶原酶Ⅶ进行造模,模型对照组和电针治疗组采用0.5u胶原酶Ⅶ注射大鼠尾壳核进行造模,确定造模成功后电针治疗组开始做治疗,分别于造模后相应的时间点开始针刺,取“水沟”、“百会”、“足三里”(双侧),接G6805—2型电针仪,疏密波,强度1mA,每次留针30min,每日电针一次,连续电针治疗5次。模型对照组不做治疗,在造模后的6h、24h、48h、72h、8天相应的时间点取材,电针治疗组分别在造模后相应时间开始做治疗,分别连续治疗5天后取材,进行相关指标的检测。应用干湿法检测血肿周围脑组织的含水量,用HE染色观察血肿周围组织形态学的变化,分别用免疫组化和western-blot检测ICAM、MMP-2、MMP-9、TIMP-1的表达。
结果
(1)HE染色结果：正常组和假手术组可见丰富的神经细胞,排列整齐,核仁清晰。6h模型对照组大鼠尾状核血肿区可见大片出血灶及点状出血,红细胞浸润,神经细胞坏死,髓质可见部分片状间质轻度水肿；24h可见大量的空泡,中性粒细胞和淋巴细胞浸润现象,炎症反应明显；48h血肿周围脑组织水肿严重,可见大量小胶质细胞浸润和明显的炎性细胞浸润；72h脑组织内脑组织水肿和细胞间隙空化最为明显,周围可见较多和炎性细胞浸润；8天模型对照组血肿周围轻度水肿；6h电针治疗组脑组织内可见点状出血,细胞间质仍有轻度水肿,可见部分中性粒细胞浸润和胶质细胞；与6h模型对照组比较相似。24h电针治疗组脑组织内可见水肿明显减轻,坏死灶明显缩小、极少量出血,有一定的中性粒细胞浸润。48h电针治疗组,水肿明显减轻,可见少量见中性粒细胞浸润,72h电针治疗组可见水肿基本消失,与8天模型对照组比较有明显的好转。电针治疗能明显减轻炎症反应,减少炎症细胞的浸润。
(2)脑出血大鼠各时间点脑组织含水量的结果：6h模型对照组脑含水量有一定程度的增加,24h组脑含水量明显增高,72h时含水量达到高峰,在8天时逐渐下降,模型对照组与正常组和假手术组比较有显著性差异(P<0.01),而相应时间点的电针治疗组与模型对照组比较：6h电针治疗组与6h模型对照组比较,差异无统计学意义(P>0.05),与8天模型对照组比较,差异无统计学意义(P>0.05)；其余相应时间点的电针治疗组与模型对照组比较有显著性的差异(P<0.01),与8天模型对照组比较有显著性的统计学意义(P<0.01)。除了6h电针治疗组,其余时间点进行电针治疗能明显减轻脑出血大鼠脑组织的含水量,减轻脑水肿。
(3) ICAM-1免疫组化和免疫印迹结果显示：模型对照组的各个时间点血肿周围组织均有不同程度ICAM-1阳性反应的内皮细胞和神经细胞,脑出血后6h周围血管和神经细胞均有ICAM-1的表达,24h、48h ICAM-1表达持续增加,72h达高峰,至8d仍有较高水平的表达。各电针治疗组与对应时间点的模型对照组ICAM-1的表达比较有显著性的差异(P<0.01),6h电针治疗组与6h模型对照组比较差异有统计学意义(P<0.01),但是作用却是增加了ICAM-1的表达,与8天组比较无统计学意义(P>0.05)；其余电针治疗组与对应时间点的模型对照组ICAM-1的表达比较有显著性的差异(P<0.01),与8天组比较有显著性的差异(P<0.05)。除了6h电针治疗组,其余时间点进行电针治疗能降低脑出血后大鼠脑组织中ICAM-1的表达。
(4)脑组织中MMP-9.TIMP-1表达结果显示：正常组未见明显的MMP-9、 TIMP-1的表达,模型对照组6h可见MMP-9明显表达,24h表达逐渐增强,在48h组达到最高峰,在8天时有少量水平的低表达；模型对照组TIMP-1的表达与MMP-9各组的表达相似。经过电针治疗后,除6h电针治疗组外电针治疗组各时间点MMP-9的表达较模型对照组的各时间点比较都有明显的减少,而TIMP-1表达较模型对照组各时间点比较都有明显的增加。统计学的比较：6h电针治疗组分别与6h模型对照组和8天模型对照组比较都无统计学意义(P>0.05),而其余各电针治疗组与对应时间点的模型对照组的MMP-9、TIMP-1的表达比较,存在着显著性的差异(P<0.01),24h、48h、72h组电针治疗组与8天模型对照组比较都有显著性的差异(P<0.05),电针能降低脑出血大鼠血肿周围MMP-9和升高TIMP-1的表达。
(5)脑组织中MMP-2表达结果显示：正常组与假手术组MMP-2的表达无明显差别(P>0.05),各模型对照组较假手术组和正常组都有显著性的差异(P<0.01)；6h模型对照组可见MMP-2的明显表达,24h持续增高至最高峰,48h-72h逐渐减少,但仍明显高于正常组,8天还有高水平的表达；经电针治疗后,除6h电针治疗组外,其余电针治疗组各时间点MMP2的表达明显的减少,6h电针治疗组分别与6h模型对照组和8天模型对照组比较,MMP-2的表达均无统计学意义(P>0.05)；其余电针治疗组与对应时间点的模型对照组MMP-2的表达比较有显著性的差异(P<0.01),与8天模型对照组比较有显著性的差异(P<0.01)。电针能够降低脑出血大鼠血肿周围组织MMP-2的表达。
结论
本研究表明在脑出血后的急性期选取百会、水沟、足三里进行电针治疗,除6h电针治疗组外,其余各时间点电针治疗均能明显降低脑出血大鼠血肿周围组织ICAM-1、MMP-9、MMP-2的表达,升高TIMP-1的表达,从而发挥抗脑出血后炎症反应的作用,是针灸具有多靶点、多通道作用于炎症细胞和神经元的结果。
Objective
By invstergation the dynamic changes of inflammatory factors surrounding hematoma after Cerebral hemorrhage and its impact of electro-acupuncture therapy at different time points, comprehend the mechanism of inflammatory factors atfter ICH, study the possible intervention mechanism about inflammatory immune response of the electroacupuncture treatment after acute cerebral hemorrhage.
Methods
Choosing120male Wistar rats were randomly divided into normal group, sham-operated group, model group and EA group, normal group, sham-operated group, with18rats each team, the model groups were divided into6h,24h,48h,72h,8days5points, with18rats each team, EA groups were divided into, with18rats each team. the model groups and EA groups Use the0.5u collagenase VII injected rats caudate nucleus to make the modelof experimental, we applied the baihui、shuigou、zusanli (both sides) for the EA group, In the corresponding time points after modeling6h,24h,48h,72h,the EA begined to Scalp-acupuncture therapy (Continuous-wave frequency of120beats/min, intensity of1mA, the treatment time30min, Once a day, five days in a row.) there wasn't any treatment of other groups.In the corresponding time points rats were killed to detect of relevant indicators, Including to measure the brain water content by dry-wet weigh method and observe the morphological changes of perihematomaby He staining, The others were to measure the protein expressions of I CAM、MMP-2、MMP-9、 TIMP-1by Immunohistochemical and Western blot
Result
(1)The results of HE staining indicate:There were no significant swelling of normal group and sham operation group of nerve cells, arranging in neat rows, structural integrity and a clear nucleolus. The model group6h rat caudate nucleus hematoma can be seen large areas of hemorrhage and spotting red blood cell infiltration, necrosis of nerve cells, the medulla visible interstitial part of the sheet with mild edema;24h can be seen a large number of vacuoles, neutrophil and lymphocyte infiltration, inflammation obvious; serious48h hematoma surrounding brain edema, seen a large number of microglia infiltration and inflammatory cell infiltration;72h brain tissue within the brain tissueedema and cell gap cavitation is most evident around can be seen more and inflammatory cell infiltration; eight days of the model group with mild edema around the hematoma;6h EA group of brain tissue seen in spotting, stromal cells, any mild edema, visible part of the neutrophil infiltration and glial cells; with the model group6h is quite similar. Visible edema within the brain tissue of24h EA group significantly reduced necrosis significantly reduced, very small amount of bleeding, certain degree of neutrophil infiltration. 48h EA group, the edema was reduced, visible see a small amount of neutrophil infiltration,72h EA group visible edema disappeared, with the model group, a marked improvement in eight days. EA treatment can significantly reduce inflammation, reduce the infiltration of inflammatory cells.
(2) The water content of brain of Cerebral hemorrhage rats at each time point tissue indicate:6h model group, brain water content increase,24h, the bleeding side of the brain water content was significantly increased, peaked at72h,24-72h to maintain a high level and then decreased, a significant difference (P<0.01) compared with normalthe corresponding point in time the EA group compared with model group, EA group6h6h model group no significant difference between the rest of the EA of the corresponding point in time with the model group and there was significant sex difference (P<0.01), compared with eight days of model group was statistically significant (P<0.05),6h,24h,72h, the EA group after electro-acupuncture treatment can significantly reduce the water content of brain tissue among the groups no significant sexdifferences.
(3) ICAM-1immunohistochemistry and Western blot results show: Model groups perihematoma have varying degrees of ICAM-1positive endothelial cells and neurons,6hours after intracerebral hemorrhage around blood vessels and nerve cells are the expression of ICAM-1,24h,48h, ICAM-1expression continued to increase,72hreached the peak at a higher level of expression in the8d any. EA group compared with the corresponding time point of the model group, ICAM-1expression, a significant sex difference (P<0.01),6h group of eight days of the EA group was not statistically significant (P>0.05), EA24h group,48h,72h group and8days group significantly with sex differences (P<0.05), while between EA24h group,48h,72h group group comparison no obvious significant difference (P>0.05), electro-acupuncture can reduce ICAM1expression
(4) MMP-9、TIMP-limmunohistochemistry and Western blot results show:Normal group, no significant MMP-9, TIMP-1expression, the model control group6h visible significant expression of MMP-9,24h expression gradually increased and reached a peak at48h group, a small amount of low level of expression at day8; model controlgroup of TIMP-1expression and MMP-9expression of each group. After electro-acupuncture treatment in addition to the6h group EA, the EA treatment group time points of MMP-9expression compared with the model control group at each time point comparison has significantly reduced; In addition to the6h group of electro-acupuncture, electro-acupuncture treatment group a TIMP-1expression, the point in time relatively significant increase compared with the model control group at each time point. Statistical comparison:6h group of electro-acupuncture treatment control group6h group and8days respectively, and the model comparisons are not statistically significant (P>0.05), while the rest of the EA group and the corresponding time point of the model control group, MMP-9of TIMP-1of expression exist with significant differences (P<0.01), EA24h group,48h,72h group and8days group has significantly with the nature of the differences (<0.05), electro-acupuncture can reduce the cerebral hemorrhage around the hematoma in rats, MMP-9and increased TIMP-1expression.
(5) MMP2immunohistochemistry and Western blot results show: Normal group and sham operation group, the expression of MMP-2was no significant difference (P>0.05), each model group compared with the sham group and normal group has a significant sex difference (P<0.01); model control group6h visible MMP-2a significant expression to24h continued to increase to the highest peak,48h-72h gradually reduced, but still significantly higher than the normal group, eight days, there is a high level of expression; after electro-acupuncture therapy, except for electro-acupuncture treatment group6h, and the remaining EAsignificantly reduce the treatment group at each time point MMP2in6h group of electro-acupuncture treatment6h group and8days compared with the model control, MMP-2expression were not statistically significant (P>0.05); the rest of the EA treatment groupcompared with the model control group the corresponding time points of MMP-2expression of differences (P<0.01), compared with eight days of model control group, significant sex differences (P<0.01). EA can reduce intracerebral hemorrhage in rats hematoma surrounding tissue expression of MMP-2.
Conclution:The study shows that after the acute phase of cerebral hemorrhage, electro-acupuncture treatment has significantly reduce ICAM-1, MMP-9, MMP-2expression and increased TIMP-1expression,So that induced anti-inflammatory response after anti-cerebral hemorrhage, which is the result of acupuncture's multi-target, multi-channel role
引文
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