褐煤中微生物筛选及胶红酵母对细粒煤的表面改性作用
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摘要
研究开发作用力强、选择性好和无毒的浮选药剂是当前细粒煤洗选研究的重要方向之一。本研究以褐煤为分离源,采用传统微生物分离法,筛选分离到一株能有效改变细粒煤表面特性的菌株KDY21,菌落形态、生理生化特征和分子序列片段分析等结果表明菌株为胶红酵母(Rhodotorula mucilaginosa),经培养条件优化后菌体量增加了32%.生长动力学设计分析以CSTR进行培养时菌体细胞产率为1.5g/(L·h).以4种不同变质程度的细粒煤为主要研究对象,通过对菌株KDY21作用前后煤样的Zeta电位、接触角及菌体吸附量测定分析,菌株KDY21处理后可显著提高实验煤样的浮选效果。影响煤样对菌体吸附量的因素依次为pH值＞煤样粒度＞接触时间＞菌液浓度,其中pH值决定了细胞与煤表面带电性的差异。另外,利用扩展DLVO理论探讨了胶红酵母KDY21对细粒煤分散体系稳定性作用的机理,揭示了在不同条件下菌株KDY21分别通过静电吸附和疏水相互作用等改变了细粒煤表而特性的同时,实现了对其选择性浮选或絮凝。
One of the important directions of the research on coal washing is developing the non-toxic flotation reagents with strong force and high selectivity. In this paper, some microorganisms with suface mldification to fine coals were screened by traditional microorganism separation methods from the lignite, and one of them designated KDY21was the best. Colony morphology, physiological and biochemical characteristics and some molecular sequence fragments showed that the strain KDY21was Rhodotorula mucilaginosa. Through the optimization culture conditition the harvest yield of strain KDY21was increased by32%. The KDY21cell production rate cultured with CSTR was1.5g/(L-h) after designing its growth dynamics. Then the surface modification to fine coals by KDY21was tested for the4kinds of different coalification range fine coals. Through the Zeta potential and contact angle measurement it was proved that using the KDY21could heighten the floatation rate greatly through changing the surface characteristics of coal. Cell adsorbing capacity on the coal surface was measured and the results showed that the order of influence factors was pH> coal particle size> contact time> concentration of bacilli. The pH determined the charging difference between cell and surface of coal. In addition, the mechanism of Rhodotorula mucilaginosa KDY21effects on the stability of fine coal disperse system had been discussed by using extended DLVO theory. All these results indicated that the modified flotation and flocculation rate of fine coal particles were improved using the strain KDY21adhered onto the fine coal surface through the electrostatic interactions and the hydrophobic interactions in different conditions.
One of the important directions of the research on coal washing is developing the non-toxic flotation reagents with strong force and high selectivity. In this paper, some microorganisms with suface mldification to fine coals were screened by traditional microorganism separation methods from the lignite, and one of them designated KDY21was the best. Colony morphology, physiological and biochemical characteristics and some molecular sequence fragments showed that the strain KDY21was Rhodotorula mucilaginosa. Through the optimization culture conditition the harvest yield of strain KDY21was increased by32%. The KDY21cell production rate cultured with CSTR was1.5g/(L-h) after designing its growth dynamics. Then the surface modification to fine coals by KDY21was tested for the4kinds of different coalification range fine coals. Through the Zeta potential and contact angle measurement it was proved that using the KDY21could heighten the floatation rate greatly through changing the surface characteristics of coal. Cell adsorbing capacity on the coal surface was measured and the results showed that the order of influence factors was pH> coal particle size> contact time> concentration of bacilli. The pH determined the charging difference between cell and surface of coal. In addition, the mechanism of Rhodotorula mucilaginosa KDY21effects on the stability of fine coal disperse system had been discussed by using extended DLVO theory. All these results indicated that the modified flotation and flocculation rate of fine coal particles were improved using the strain KDY21adhered onto the fine coal surface through the electrostatic interactions and the hydrophobic interactions in different conditions.
引文
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