吉林白鹅α/γIFN原核表达、抗病毒活性及其核酸疫苗免疫佐剂作用的研究
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摘要
由于干扰素具有良好的广谱抗病毒活性和有效的免疫调节功能,对于研发有效的抗病毒生物制剂及增强疫苗效果的佐剂具有重要的意义。本研究利用基因工程技术,以吉林白鹅α/γ干扰素基因(JGIFN-α/γ)为研究对象,对其进行了克隆、表达及抗病毒活性研究。同时为了进一步研究JGIFN-γ作为佐剂的免疫增强作用,成功构建了带有分子免疫佐剂JGIFN-γ的GPV-VP3基因疫苗并对雏鹅进行了免疫,为研发高效的鹅细小病毒基因疫苗奠定了基础,本研究具体进行了以下几方面的工作：
1、吉林白鹅α干扰素成熟肽原核表达及抗病毒活性的研究
参照已克隆得到的JGIFN-α完整的ORF基因序列设计了一对特异性引物,克隆得到了编码α干扰素的成熟肽基因(mJGIFN-a)。利用pET28a (+)作为原核表达载体,成功构建了吉林白鹅α干扰素基因的原核表达质粒pET-mJGIFN-a。将该重组表达质粒转化入E.coli Rossetta菌中进行了表达,经IPTG诱导、SDS-PAGE和western-blot分析表明获得了分子量为22ku的包涵体蛋白。将包涵体蛋白经Ni-IMAC亲和层析法纯化及稀释透析复性后,得到了与预期结果一致nJGIFN-a重组蛋白。采用微量细胞病变抑制法分析mJGIFN-a重组蛋白的抗病毒活性,表明其抗病毒活性为7.9×102U/mL,比活性为1.3×103U/mg。同时通过FQ-PCR方法研究动态检测抗鹅细小病毒(GPV)的生物活性,结果发现该重组蛋白能够抑制GPV的复制,具有明显的抗病毒活性,为制备应用于临床上的抗病毒生物制剂研究提供了科学依据。
2、吉林白鹅γ干扰素基因序列分析、原核表达及抗病毒活性
采用RT-PCR的方法从吉林白鹅外周血单核细胞中扩增得到了Y干扰素基因(JGIFN-γ),构建了重组克隆质粒pMD-JGIFN-γ。利用生物信息学软件及在线分析软件对JGIFN-γ基因的核酸及氨基酸序列进行了分析。分析13个不同动物的相关基因结果表明JGIFN-γ与同为水禽的鸭、鹅的核苷酸和氨基酸同源性均高于90%,在进化树上位于同一分支。JGIFN-γ蛋白含有4个潜在的疏水区、3个N-糖基化位点、10个磷酸化位点。对其亚细胞定位预测结果为大部分蛋白分布于在细胞核内,其余分别在线粒体、细胞质、质膜上、内质网上、高尔基氏体及细胞外,为进一步研究γ干扰素蛋白的功能特性提供了理论依据。二级结构预测结果显示该肽链主要由α-螺旋组成,包括4个抗原指数较高的抗原表位,表明γ干扰素具有良好的抗原性,为进一步研究其作为分子佐剂提供了理论依据。同时成功的构建了原核表达质粒pET-JGIFN-γ,获得了JGIFN-γ重组蛋白,将该重组蛋白经Ni-IMAC亲和纯化及稀释透析复性后,采用微量细胞病变抑制发分析其抗病毒活性,结果表明其抗病毒活性为1.9×102U/mL,比活性为4.3×102U/mg,为研究γ干扰素蛋白的生物学活性提供了科学依据。
3、吉林白鹅IFN-γ及其与VP3融合基因核酸疫苗的制备与表达研究
通过重叠延伸PCR (SOE-PCR)技术得到包含了缺失TAA终止密码子的JGIFN-γ基因和缺失了ATG起始密码子的VP3的融合基因JGIFN-γ-VP3,两个基因之间由高亲水性氨基酸Gly-Gly-Gly-Ser编码的核苷酸连接起来。分别将JGIFN-γ和JGIFN-γ-VP3融合基因通过基因重组技术正向插入到pVAX1CMV启动子下游BamHI和HindⅢ酶切位点之间,成功构建了真核重组表达质粒pVAX-JGIFN-γ和pVAX-JGIFN-γ-VP3。利用脂质体介导法将其转入Vero细胞中,通过间接免疫荧光技术和RT-PCR技术证实JGIFN-γ基因和JGIFN-γ-VP3融合基因在Vero细胞中获得了表达,为进一步研究γ干扰素基因作为基因疫苗的分子佐剂奠定了基础。
4、GIFNγ-VP3真核表达质粒对雏鹅的免疫研究
为研究真核重组表达质粒pVAX-JGIFN-γ+pVAX-VP3/pVAX-JGIFNγ-VP3免疫调节特性,将不同剂量pVAX-JGIFN-γ-VP3质粒和不同剂量pVAX-JGIFN-γ质粒与pVAX-VP3质粒联合使用免疫28日龄鹅。同时设pVAX-VP3质粒免疫组、小鹅瘟弱毒疫苗、pVAX1、生理盐水对照组。对不同时间点鹅外周血淋巴细胞增殖试验的结果表明,pVAX-JGIFN-γ-VP3基因免疫组细胞免疫水平高于pVAX-JGIFN-γ+pVAX-VP3基因疫苗免疫组,同类疫苗中且呈现剂量相关性。分子佐剂基因疫苗免疫组显著高于pVAX-VP3基因疫苗免疫组但显著低于GPV弱毒疫苗组(P<0.05)。间接ELISA方法检测鹅体内IgG动态变化规律结果表明,pVAX-JGIFN-γ-VP3和pVAX-JGIFN-γ+pVAX-VP3所诱导的体液免疫的抗体水平,明显高于pVAX-VP3单基因疫苗免疫组(P<0.05),但显著低于GPV弱毒疫苗组(P≤0.05)。同时采用微量血清中和抗体试验检测了各组疫苗免疫后所诱导的中和抗体水平结果表明,各基因疫苗和GPV弱毒株均能较长时间诱导雏鹅产生针对GPV的中和抗体,含分子佐剂GPV-VP3基因疫苗的中和抗体水平比不含佐剂的GPV-VP3基因疫苗组高；而注射pVAX1和生理盐水的对照组均不能保护GPV对鹅成纤维细胞的感染,说明了DNA基因疫苗和GPV弱毒疫苗免疫所诱导的中和抗体均在一定程度上可以保护GEF不被GPV感染。
Interferon has broad-spectrum antiviral activity and effective immunoregulation. So it has important significance to develop effective antiviral biological agent and enhanced vaccine adjuvant.JGIFN-α/γ has been cloned, expressed studied antiviral activity in this study. Further study on JGIFN-γ of immunological enhancement.Carrying molecular immune adjuvant DNA vaccine has been constructed and immuned to gosling.The study laid a foundation for goose parvovirus DNA vaccine.the study included as follows:
1. The research of prokaryotic expression and antiviral activity of mJGIFN-a
The mature peptide gene was amplified by one pair specificity primer based on the cloned. Then the mJGIFN-a was cloned into pET-28a(+) vector and constructed prokaryotic expression plasmid pET-mJGIFN-a.The22ku expressed protein was obtained Ni-IMAC after affinity chromatography and dilution dialysis renaturation. The anti-VSV of recombinant protein mJGIFN-a activity by micro cytopathogenic effect inhibition assay was1.3×103U/mg.It was detected the anti-GPV of recombinant protein by FQ-PCR.The resuts showed the antivity of mJGIFN-a to GPV protein was detected.
2. Sequence analysis and expression and antivirus activity detection of JGIFN-γ
The Jilin white goose interferon-gamma (JG-IFN-γ) was amplified from ConA-stimulated peripheral blood mononuclear cells (PBMCs) by RT-PCR. Then constructed the recombinant plasmid (pMD-JGINF-γ) sucessfully. The nucleotide and the deduced amino acid sequence of JGINF-γ were analyzed by bioinformatics software and on-line tools. The homology with13other correlative gene sequences was analyzed. The homology showed that the JGINF-y has more than90%similarity to waterfowl (duck and goose) and in the same branch in the phylogenetic tree. It has the most homology with dometic goose of which99.6%nucleotide and97.6%amino acid. The protein wasl9ku.The results analysise showed three N-glycosylation sites, ten potential phosphorylation sites, four potential hydrophobic regions were in the JGIFN-γ amino acid.The analysis of the protein subcellular location indicated that the JGIFN-γ mostly located at nuclear, others located at mitochondrial, cytoplasmic, plasma membrane, vesicles of secretory system, extracellular,including cell wall and Golgi.The secondary structure prediction results showed that JGIFN-y was composed of a-helix with four epitopes of higher antigen index. Then JGIFN-y was constructed prokaryotic expression plasmid (pET-JGIFN-γ) and expressed in strain rossetta. The22ku protein was obtained after Ni-IMAC affinity chromatography and dilution dialysis renaturation.The anti-VSV activity of recombinant protein by micro cytopathogenic effect inhibition assay was4.3×102U/mg.
3. The study of eukaryotic expression and preparation of JGIFN-γ/JGIFN-γ-VP3DNA gene vaccine
JGIFN-γ-VP3fusion gene which lacked TAA stop codon of JGIFN-γ and ATG iniation codon of VP3gene and linked by high hydrophilia amino acid (Gly-Gly-Gly-Ser) encoding nucleotides was amplied by using spicing by overlap extention. Then JGIFN-γ-VP3fusion gene and JGIFN-γ was inserted into pVAX1under the control of CMV respectively. pVAX-JGIFN-γ and pVAX-JGIFN-γ-VP3were constructed successfully,which were then transfected into the vero cells by lipofection transfection.
4. The immunity study of eukaryotic expression plasid JGIFN-γ-VP3in the goslings
In order to study on the immunity fuction of pVAX-JGIFN-γ and p VAX-JGIFN-γ-VP3. The pVAX-JGIFN-γ-VP3of the different doses and the pVAX-JGIFN-γ of the different doses with pVAX-VP3DNA vaccines was immunized to28-day old geese. The VP3DNA vaccine,the empty vector plasmid pVAX1, Gosling Plague Attenuated vaccine and saline was served as control group. The anticoagulated blood samples were harvested at different times to detect the proliferation of geese T lymphocytes in PBMCs by T lymphocyte proliferation tests(MTT).The results showed that the pVAX-JGIFN-γ-VP3immunized groups were higher than the same dose of pVAX-JGIFN-y+pVAX-VP3immunized groups. The adjuvant groups significantly higher than (P<0.01) higher than pVAX-VP3DNA vaccine group,but lower than GPV attenuated groups. The kinetics of IgG of serum samples which were collect from immunized geese was detected by indirect ELISA. The results showed that ELISA antibody of humoral immunity induced by pVAX-JGIFNy-VP3and pVAX-JGIFNy+pVAX-VP3was higher than pVAX-VP3DNA vaccine. The GPV attenuated groups were higher than all of DNA vaccine. The neutralizing antibody level of pVAX-JGIFNy-VP3and pVAX-JGIFNy+pVAX-VP3immuized was detected by virus neutralization test. The results showed neutralizing antibody of all of vaccines was produced.the adjuvant of GPV-VP3DNA vaccines were significant higher than GPV-VP3DNA vaccine. The neutralizing antibody level induced by three kinds of GPV DNA vaccines and GPV attenuated vaccine maintained longer times. but the adjuvant DNA vaccine groups was higher than DNA vaccine groups.The neutralizing antibody induced by all of vaccines can protect GEF from infecting GPV to some extent
1、吉林白鹅α干扰素成熟肽原核表达及抗病毒活性的研究
参照已克隆得到的JGIFN-α完整的ORF基因序列设计了一对特异性引物,克隆得到了编码α干扰素的成熟肽基因(mJGIFN-a)。利用pET28a (+)作为原核表达载体,成功构建了吉林白鹅α干扰素基因的原核表达质粒pET-mJGIFN-a。将该重组表达质粒转化入E.coli Rossetta菌中进行了表达,经IPTG诱导、SDS-PAGE和western-blot分析表明获得了分子量为22ku的包涵体蛋白。将包涵体蛋白经Ni-IMAC亲和层析法纯化及稀释透析复性后,得到了与预期结果一致nJGIFN-a重组蛋白。采用微量细胞病变抑制法分析mJGIFN-a重组蛋白的抗病毒活性,表明其抗病毒活性为7.9×102U/mL,比活性为1.3×103U/mg。同时通过FQ-PCR方法研究动态检测抗鹅细小病毒(GPV)的生物活性,结果发现该重组蛋白能够抑制GPV的复制,具有明显的抗病毒活性,为制备应用于临床上的抗病毒生物制剂研究提供了科学依据。
2、吉林白鹅γ干扰素基因序列分析、原核表达及抗病毒活性
采用RT-PCR的方法从吉林白鹅外周血单核细胞中扩增得到了Y干扰素基因(JGIFN-γ),构建了重组克隆质粒pMD-JGIFN-γ。利用生物信息学软件及在线分析软件对JGIFN-γ基因的核酸及氨基酸序列进行了分析。分析13个不同动物的相关基因结果表明JGIFN-γ与同为水禽的鸭、鹅的核苷酸和氨基酸同源性均高于90%,在进化树上位于同一分支。JGIFN-γ蛋白含有4个潜在的疏水区、3个N-糖基化位点、10个磷酸化位点。对其亚细胞定位预测结果为大部分蛋白分布于在细胞核内,其余分别在线粒体、细胞质、质膜上、内质网上、高尔基氏体及细胞外,为进一步研究γ干扰素蛋白的功能特性提供了理论依据。二级结构预测结果显示该肽链主要由α-螺旋组成,包括4个抗原指数较高的抗原表位,表明γ干扰素具有良好的抗原性,为进一步研究其作为分子佐剂提供了理论依据。同时成功的构建了原核表达质粒pET-JGIFN-γ,获得了JGIFN-γ重组蛋白,将该重组蛋白经Ni-IMAC亲和纯化及稀释透析复性后,采用微量细胞病变抑制发分析其抗病毒活性,结果表明其抗病毒活性为1.9×102U/mL,比活性为4.3×102U/mg,为研究γ干扰素蛋白的生物学活性提供了科学依据。
3、吉林白鹅IFN-γ及其与VP3融合基因核酸疫苗的制备与表达研究
通过重叠延伸PCR (SOE-PCR)技术得到包含了缺失TAA终止密码子的JGIFN-γ基因和缺失了ATG起始密码子的VP3的融合基因JGIFN-γ-VP3,两个基因之间由高亲水性氨基酸Gly-Gly-Gly-Ser编码的核苷酸连接起来。分别将JGIFN-γ和JGIFN-γ-VP3融合基因通过基因重组技术正向插入到pVAX1CMV启动子下游BamHI和HindⅢ酶切位点之间,成功构建了真核重组表达质粒pVAX-JGIFN-γ和pVAX-JGIFN-γ-VP3。利用脂质体介导法将其转入Vero细胞中,通过间接免疫荧光技术和RT-PCR技术证实JGIFN-γ基因和JGIFN-γ-VP3融合基因在Vero细胞中获得了表达,为进一步研究γ干扰素基因作为基因疫苗的分子佐剂奠定了基础。
4、GIFNγ-VP3真核表达质粒对雏鹅的免疫研究
为研究真核重组表达质粒pVAX-JGIFN-γ+pVAX-VP3/pVAX-JGIFNγ-VP3免疫调节特性,将不同剂量pVAX-JGIFN-γ-VP3质粒和不同剂量pVAX-JGIFN-γ质粒与pVAX-VP3质粒联合使用免疫28日龄鹅。同时设pVAX-VP3质粒免疫组、小鹅瘟弱毒疫苗、pVAX1、生理盐水对照组。对不同时间点鹅外周血淋巴细胞增殖试验的结果表明,pVAX-JGIFN-γ-VP3基因免疫组细胞免疫水平高于pVAX-JGIFN-γ+pVAX-VP3基因疫苗免疫组,同类疫苗中且呈现剂量相关性。分子佐剂基因疫苗免疫组显著高于pVAX-VP3基因疫苗免疫组但显著低于GPV弱毒疫苗组(P<0.05)。间接ELISA方法检测鹅体内IgG动态变化规律结果表明,pVAX-JGIFN-γ-VP3和pVAX-JGIFN-γ+pVAX-VP3所诱导的体液免疫的抗体水平,明显高于pVAX-VP3单基因疫苗免疫组(P<0.05),但显著低于GPV弱毒疫苗组(P≤0.05)。同时采用微量血清中和抗体试验检测了各组疫苗免疫后所诱导的中和抗体水平结果表明,各基因疫苗和GPV弱毒株均能较长时间诱导雏鹅产生针对GPV的中和抗体,含分子佐剂GPV-VP3基因疫苗的中和抗体水平比不含佐剂的GPV-VP3基因疫苗组高；而注射pVAX1和生理盐水的对照组均不能保护GPV对鹅成纤维细胞的感染,说明了DNA基因疫苗和GPV弱毒疫苗免疫所诱导的中和抗体均在一定程度上可以保护GEF不被GPV感染。
Interferon has broad-spectrum antiviral activity and effective immunoregulation. So it has important significance to develop effective antiviral biological agent and enhanced vaccine adjuvant.JGIFN-α/γ has been cloned, expressed studied antiviral activity in this study. Further study on JGIFN-γ of immunological enhancement.Carrying molecular immune adjuvant DNA vaccine has been constructed and immuned to gosling.The study laid a foundation for goose parvovirus DNA vaccine.the study included as follows:
1. The research of prokaryotic expression and antiviral activity of mJGIFN-a
The mature peptide gene was amplified by one pair specificity primer based on the cloned. Then the mJGIFN-a was cloned into pET-28a(+) vector and constructed prokaryotic expression plasmid pET-mJGIFN-a.The22ku expressed protein was obtained Ni-IMAC after affinity chromatography and dilution dialysis renaturation. The anti-VSV of recombinant protein mJGIFN-a activity by micro cytopathogenic effect inhibition assay was1.3×103U/mg.It was detected the anti-GPV of recombinant protein by FQ-PCR.The resuts showed the antivity of mJGIFN-a to GPV protein was detected.
2. Sequence analysis and expression and antivirus activity detection of JGIFN-γ
The Jilin white goose interferon-gamma (JG-IFN-γ) was amplified from ConA-stimulated peripheral blood mononuclear cells (PBMCs) by RT-PCR. Then constructed the recombinant plasmid (pMD-JGINF-γ) sucessfully. The nucleotide and the deduced amino acid sequence of JGINF-γ were analyzed by bioinformatics software and on-line tools. The homology with13other correlative gene sequences was analyzed. The homology showed that the JGINF-y has more than90%similarity to waterfowl (duck and goose) and in the same branch in the phylogenetic tree. It has the most homology with dometic goose of which99.6%nucleotide and97.6%amino acid. The protein wasl9ku.The results analysise showed three N-glycosylation sites, ten potential phosphorylation sites, four potential hydrophobic regions were in the JGIFN-γ amino acid.The analysis of the protein subcellular location indicated that the JGIFN-γ mostly located at nuclear, others located at mitochondrial, cytoplasmic, plasma membrane, vesicles of secretory system, extracellular,including cell wall and Golgi.The secondary structure prediction results showed that JGIFN-y was composed of a-helix with four epitopes of higher antigen index. Then JGIFN-y was constructed prokaryotic expression plasmid (pET-JGIFN-γ) and expressed in strain rossetta. The22ku protein was obtained after Ni-IMAC affinity chromatography and dilution dialysis renaturation.The anti-VSV activity of recombinant protein by micro cytopathogenic effect inhibition assay was4.3×102U/mg.
3. The study of eukaryotic expression and preparation of JGIFN-γ/JGIFN-γ-VP3DNA gene vaccine
JGIFN-γ-VP3fusion gene which lacked TAA stop codon of JGIFN-γ and ATG iniation codon of VP3gene and linked by high hydrophilia amino acid (Gly-Gly-Gly-Ser) encoding nucleotides was amplied by using spicing by overlap extention. Then JGIFN-γ-VP3fusion gene and JGIFN-γ was inserted into pVAX1under the control of CMV respectively. pVAX-JGIFN-γ and pVAX-JGIFN-γ-VP3were constructed successfully,which were then transfected into the vero cells by lipofection transfection.
4. The immunity study of eukaryotic expression plasid JGIFN-γ-VP3in the goslings
In order to study on the immunity fuction of pVAX-JGIFN-γ and p VAX-JGIFN-γ-VP3. The pVAX-JGIFN-γ-VP3of the different doses and the pVAX-JGIFN-γ of the different doses with pVAX-VP3DNA vaccines was immunized to28-day old geese. The VP3DNA vaccine,the empty vector plasmid pVAX1, Gosling Plague Attenuated vaccine and saline was served as control group. The anticoagulated blood samples were harvested at different times to detect the proliferation of geese T lymphocytes in PBMCs by T lymphocyte proliferation tests(MTT).The results showed that the pVAX-JGIFN-γ-VP3immunized groups were higher than the same dose of pVAX-JGIFN-y+pVAX-VP3immunized groups. The adjuvant groups significantly higher than (P<0.01) higher than pVAX-VP3DNA vaccine group,but lower than GPV attenuated groups. The kinetics of IgG of serum samples which were collect from immunized geese was detected by indirect ELISA. The results showed that ELISA antibody of humoral immunity induced by pVAX-JGIFNy-VP3and pVAX-JGIFNy+pVAX-VP3was higher than pVAX-VP3DNA vaccine. The GPV attenuated groups were higher than all of DNA vaccine. The neutralizing antibody level of pVAX-JGIFNy-VP3and pVAX-JGIFNy+pVAX-VP3immuized was detected by virus neutralization test. The results showed neutralizing antibody of all of vaccines was produced.the adjuvant of GPV-VP3DNA vaccines were significant higher than GPV-VP3DNA vaccine. The neutralizing antibody level induced by three kinds of GPV DNA vaccines and GPV attenuated vaccine maintained longer times. but the adjuvant DNA vaccine groups was higher than DNA vaccine groups.The neutralizing antibody induced by all of vaccines can protect GEF from infecting GPV to some extent
引文
[1]Issacs A.Lindermann J.Virus interference:The Interferon[J].Proc R Soc Lond Ser.1957.147:258-263
[2]Lampson GP.Tytell AA.Nemes MM.et al.Purification and characterization of chick embryo interferon [J].Proc Soc Exp Biol Med.1963,112:468-78
[3]Gray P W.Peung D W.Pennica D.et al.Expression of human immune interferon cDNA in E.coli and monkey cells[J].Nature.1982.295:503-506
[4]Dijkmans R,Vandenbroeck K,Beuker et al.Sequence of the porcine interferon-gamma gene[J].Nucleic Acids Research.1990.18:42-59
[5]李洪泉.干扰素抗病毒机制及其抗病毒性肝炎中的作用[J].中国公共卫生,2005,7(21)：890-891
[6]Knapp S,Yee LJ,Frodsham AJ,et al.Polymorphisms in interferon-induced genes and the outcome of hepatitis C virus infection:roles of MxA, OAS21 and PKR[J]. Genes Immun,2003,4 (6):411-419
[7]Uka K,Aikata H,Mori N,et al.Combination therapy of oral fluoropyrimidine anticancer drugS-1 and interferon alpha for HCC patients with extra hepatic metastases[J]Oncology,2008,75 (1-2):8-16
[8]Billiau A. Interferon-gamma:biology and role in pathogenesis[J]Adv Immunol,1996,62:61-130
[9]潘烨,郑起.干扰素在肿瘤治疗中应用的研究进展[J]国外医学外科学分,2005,32(1)：10-13
[10]Avendano S.C.Interferons:types and actions.Gastroenterol[J]Hepatol.2006.29 Suppl.2:125-128
[11]Lefevre.E.Interferon-delta the first member of a novel type interferon family[J].Biochimic 1998,80 (8-9):779-788
[12]Ishida N,Oritani K,Shiraga M et al. δDifferential effects of a novel[FN-ζlimitin and IFN-α on signals for Daxx induction and Crk phosphorylation that coup le with growth control of megakaryocytes [J].Experimental Hematology,2005,33:495-503
[13]Sheppard P, Kindsvogel W,Xu W,et al. IL-28,IL-29 and theirclass Ⅱ cytokine receptor IL-28R[J] Nat Immunol,2003,4 (1),:63-8
[14]曲建惠,张玲霞.干扰素的分子生物学机制研究进展[J].国际流行病学传染病学杂志,2006,33(1)：38-44
[15]Cheng G.,Chen W.Z.,Li Z.F,et al. Characterization of the Porcine alpha interferon multigene family[J] Gene.2006,382:28-38
[16]de Avila Botton S.,Brum M.C.S, Bautista E.et al. ImmunoPoteniiation of a foot-and-mouth disease virus subunit vaccine by interferon alpha [J].Vaccine 2006,24:3446-3456
[17]Golding,S.M.,Hedger R.S.,Talbot P.Radial immuno-diffusion and serum-neutralisation techniques for the assay of antibodies to swine vesicular disease[J]Res.Vet.Sci.1976-20:142-147
[18]孙亚萍,王英明,乔守怡.干扰素及其最新研究进展[J]中国免疫学杂志.2006,22(7)：676-679
[19]LaFleur D W, Nardelli B,Tsareva T et al. Interferon-k a novel type I interferon expressed in human keratinocytes [J]. J Biol Chem,2001,276:39765-39771
[20]Sun,T.Lu P.,Wang X.Localization of infection-related epitopes on the non-structural protein 3ABC of foot-and-mouth disease virus and the application of tandem epitopes[J].Virol.Methods,2004,119:79-86
[21]Kang,Y.M,Jin H. Xie Q.F. et al.The adjuvant effect of levamisole on Killed viral vaccines[J]. Vaccine,200523:5543-5550
[22]Langer JA,Cuortne EC,Kotenko S.The class II Cytokine receptor(CRF2)family:overview and patterns of receptor-ligand interactions[J].cytokine and growth factor reviews,2004,15:33-48
[23]韩春来.鸡干扰素与干扰素受体重组蛋白的生物学特性及其相互作用.中国农业大学博士论文,2005
[24]张纪岩.细胞因子受体超家族的结构保守性及生物学意义[J].国外医学分子生物学分册,1997,19(5)：193-196
[25]Lutfalla G, Gardiner K, Proudhon D et al The structure of the human interferon α/βreceptor gene[J].BiolChem,1992,276 (4):2802-2809
[26]Walter M R,Windsor WT, Nagabhushan TL et al.Crystal structure of a complex between interferon-yand its soluble high-affinity receptor [J].Natuer,1995,376 (20):230-35
[27]Kenji Oritani,Paul W Kincade,Cai Zhang,et al.Type I interferons and limitin:a comparison of structure,receptors and functions[J].Cytokine Growth Factor Rev.2001,12:337
[28]Lamken P.Lata S.Gavutis M et al.Ligand-induced assembling of the type I interferon receptor on supported lipid bilayers[J]Mol Biol.2004.341:303-318
[29]Langer J A,Yang J,Carmillo P et al.Bovine type I interferon protein BoIFNARl has high affinity and broad specifity for human type I interferons[J].FEBS Letters,1998,421:131-135
[30]Rhee S,Ebenspegrer C,Dembic Z et al.The structure of the gene for the second chain of the human interferon-yreceptor[J].Biol Chem,1996,271 (46):28947-28952
[31]Piehler J,Schreiber G. Fast transient cytokine-receptor interactions monitored in real time by reflectometric interference spectroscopy[J].Analytical B iochemistry,2001,289:173-186
[32]Kotenko S V. The fam ily of IL-10-related cytokines and their receptors:related, but to what extent [J]. Cytokine Growth Factor Rev,2002; 13:223-240
[33]Oritani K,Kincade PW,Zhang C et al.Type I interferons and limitin:a comparison of structures.receptors, and functions[J].Cytokine growth factor rev,2001,12:337-348
[34]Ragimbeau J,Dondi E,Alcover A et al.The tyrosine Tyk2 controls IFNAR1 cell surface expression [J]. EMBO J,2003,22(3):537-547
[35]Kumar KG,Krolewski JJ,Fuchs SY et al.Phosphorylation and specific ubiquitin-acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor[J].J Biol Chem,2004,5279(45):46614-20
[36]MuKamoto M,Kodama H,Baba T.Effects of cytokines from thymocytes and thymic stromal cells on chicken intrathymic T cell development[J].Veterinary Immunol Immunopathol,1999,67(3):223-33
[37]Pernis A,Gupta S,Gollob KJ et al.Lack of interferon-γ receptorβchain and the prevention of interferon ysignaling in Thl cells[J].Science,1995,269(5221):245-7
[38]Groux H,Sornasse T,Cottrez F et al.Induction of human T helper cell type I differentiation results in loss of IFN-y receptor (3 chain expression[J].The journal of Immunology,1997(158):5627-5631
[39]Rigamonti L,Aritti S,Losana G et al.Surface expression of the IFN-y R2 chains is regulated by intracellular trafficking in human T lymphocytes[J]. J Immunol,2000,164(1):201-7
[40]Skrenta H,Yang Y,Pestka S et al.Ligand-indepent down-regulation of IFN-y receptor 1 following TCR engagement[J].The journal of Immunology,2000,164(7):3506-11
[41]Bernabei P,Bosticardo M,Losana G et al.IGF-1 down-regulates IFN-y R2 chain surface expression and desensitizes IFN-y/STAT-1 signaling in human T lymphocytes[J].Blood,2003,102(8):2933-2939
[42]Novelli F,DElios MM,Bernabei P,et al.Expression and role in apoptosis of the alpha-and-beta-chains of the IFN-gamma receptor on human Th 1 and Th2 clones[J].The joural of Immunology,1997(159):306-213
[43]Altare F,Jouanguy E,Newport M et al.IFNGR1,a human mycobacterial susceptibility candidate gene[J].Bull Inst Pasteur,1997,95:143-146
[44]Zhang Ping,Chen Zhi,Chen Feng.et al Expression of IFN-gamma and its receptor alpha in the peripheral blood of patients with chronic hepatitis C[J]Chin Med.2004,117(1)79-82
[45]Osterlund PI,PietiIae TE, Veckman V, et al.IFN regulatory factor family members differentially regulate the expression of type Ⅲ IFN (IFN-λ.) genes.[J].J Immunol,2007,179 (6):3434-42
[46]Sadler AJ,Williams BR. Interferon-inducible antiviral effectors[J].Nat Rev Immunol,2008,8(7):559-68
[47]侯炜.Ⅲ型干扰素—新型抗病毒细胞因子[J].生命科学,2011,23,8：749-752
[48]Takaoka A.,Yanai H.Interferon signaling network in innate defense[J].Cell Microbiol,2006,8:907-922
[49]Haig DM,Poxvirus interference with the host cytokine respone[J].Vet Immunol,1998,63:149-156
[50]Leonard GT,Sen G C.Restoration of interferon response of adenovirus E1 A-expression HT1080 cell lines by overexpression of p48 protein.[J]. Virol,1997,71:5095-5101
[51]Elevy D,Adolfo G S. The virus battles:IFN induction of the antiviral state and mechanisms of viral evasion [J]. Cytokine and Growth Factor Reviews,2001, (12):143-156
[52]侯云德.分子病毒学.第二版.学苑出版社.北京：1990：201-212
[53]Samuel C.E.Antiviral actions of interferons.[J]Clin Microbiol Rev,2001,14:778-809
[54]Hall O M,G Kochs.Mx proteins:mediates of innate resistance to RNA virus[J].Rev Sci Technol Off Int Epizootol,1998,17:220-230
[55]王晓杜,猪干扰素α、β、γ基因克隆、原核表达及其单克隆抗体制备的研究.硕士毕业论文.华中农业大学,2007
[56]Tboullat D A,Hovanian GDavid, et al.Characterization of the gene coding the 100-kDa form of human 2'-5'oligoadenylate synthetas[J].Genomic,2000,70:232-240
[57]姚清侠.猪α干扰素、猪β干扰素与猪γ干扰素的抗病毒活性及其免疫佐剂的研究.博士学位论文,华中农业大学,2007
[58]Batusic D.S.et al.Induction of Mx-2 in rat liver by toxic injury[J] J Hepatol.2004,40(3):446-53
[59]于剑光.人类疱疹病毒8型[J]人类疱疹病毒8[J].国外医学：病毒学分册,2001,04：100-104
[60]Tiefenbrun N,Melamed D,Levy N,et al. Alpha interferon suppresses the cyclin D3 and cdc25A genes,leading to a reversible golike arrest[J]. Mol Cell Biol,1996,16:3934-3944
[61]Shin EC,Ahn JM,Kim CH, et al. IFN-gamma induces cell death in human hepatoma cells through a TRA 1L/death recep tor-mediated apoptotic pathway [J]. Int J Cancer,2001,93 (2):262-268
[62]Yoshiji H, Noguchi R, Kuriyama S,et al. Combination of interferon and angiotensin-converting enzyme inhibitor, perindop ril, sup-presses liver carcinogenesis and angiogenesis in mice [J].Oncol Rep, 2005,13 (3):491-495
[63]Wu W Z, Sun H C, Shen Y F, et al. Interferon alpha 2a down-regulates VEGF expression through PI3 kinase and MAP kinase signaling pathways[J]. J Cancer Res Clin Oncol,2005,131 (3):169-178
[64]Suginoshita Y, Kakimi K, et al. Impairment of IFN-a production capacity in patients with hepatitis C virus and the risk of the development of hepatocellular carcinoma[J].World J Gastroenterol,2005,11 (46):7330-7334
[65]Tosi D, Valenti R, Cora A, et al. Role of cross-talk between IFN-alpha induced momocyte-derived dendritic cells and NK cells in priming CD8+ T cell responses against human tumor antigens[J].J Imm unol,2004,172 (9):5363-5370
[66]Radaeva S, Jaruga B, Hong F, et al. Interferon-alpha activatesmultip le STAT signals and down-regulates c-Met in primary human hepatocytes[J]. Gastroenterology,2002,122 (4):1020-1034
[67]Xu D, Erickson S,Szeps M,et al. Interferon down-regulates telomerase reverse transcriptase and telomerase activity in human malignant and nonmalignant hematopoietic cells [J].Blood,2000,96 (13): 4313-4318
[68]赵明军,闫若潜,韩记用,等.猪α干扰素研究进展[J].畜禽业：南方养猪,2008,225(1)：48-51
[69]Le Bon A.Tough D F,Links between innate and adaptive immunity via type I interferon[J].Curr Opin Immunol.2002,14(4):432-6
[71]Jiang C.L.et al.Analgesic effect of interferon-alpha via mu opioid receptor in the rat[J].Neurochem Int. 2000,36(3):193-6
[72]Levy D.E,Garcia-Sastre A.The virus battles:IFN induction of the antivirual state and mechanisms of viral evasion[J].Cytokine Growth Factor Rev.2001,12(2-3):143-56
[73]马良.干扰素与寄生虫感染[J].重庆医科大学学报,1995,20(3)：241-244
[74]Min W,Lillehoj HS,Buxnside J,et al.Adjuvant effects of IL-1β, IL-2, IL-8, IL 15, IFN-a, IFN-γ, TGF-β4 and lymphotactin on DNA vaccination against Eimeria acervulina [J].Vaccine,2001,20 (1-2):267-274
[75]李延清.孙志宏.胡格.γ-干扰素与免疫-神经-内分泌网络的相互关系[J].延安大学学报(自然科学版).2003,22(3)：74-76
[76]徐天乐.张远强.细胞因子对内分泌的影响[J].国外医学·内分泌学分册.1992.12(3)：113-116
[77]Kato A,Kiyotani K,Kubota T,et al.Importance of the anti-interferon capacity of Sendai virus C protein for pathogenicity in mice[J].J Virol,2007,81(7):3264-3271
[78]Shaw M L,Garcia-Sastre A,Palese P,et al.Nipah virus V and W protein have a common STAT1-binding domain yet inhibit STAT1 activation from the cytoplasmic and nuclear compartments,respectively[J].J Virol,2004,78(11):5633-5641
[79]Kopecky-Bromberg S A,Martinez-Sobrido L,Frieman M et al.Severe acute respiratory syndrome coronavirus open reading frame(ORF)3b,ORF 6,and nucleocapsid proteins function as interferon antagonists[J].J Virol,2007,81(2):548-557
[80]Reid S P,Leung L W,Hartman A L,et al/Ebola virus VP24 binds karyopherin alphal and blocks STAT1 nuclear accumulation[J].J Virol,2006,80(11):5156-5167
[81]Sekellick M J,Ferrandino A F,Hopkins D A et al.Chicken interferon gene:cloning,expression,and analysis[J],J Interferon Res,1994,14(2):71-79
[82]Schultz, Rinderle C. Sekellick M J, et al. Recombinant chicken interferon from Escherichia coli and transfected COS cells is biologically active [J]. Europe Journal of Biochemistry.1995.229 (1):73-76
[83]夏春,汪明,朱凌云,等.惠阳胡须鸡IFN-α基因克隆和序列分析畜牧兽医学报[J].2000,31(6),563-56
[84]吕英姿,毕英佐,曹永长,等.石歧杂鸡γ-干扰素基因的克隆与序列分析华南农业大学学报[J].2002,23(4)：61-63
[85]韩春来,汪明,张灿,等.惠阳胡须鸡IFN-α基因的克隆表达和重组蛋白的抗病毒活性圆二色性分析[J].中国兽医杂志,2006,42(11)：3-6
[86]戴华,郑佳玉,陈俊华等,鸡α干扰素基因的原核表达及其活性测定[J]中国预防兽医学报,2009,31,10：805-807
[87]陈伟业.葛金英,肇慧君.基于Mx启动子和荧光素酶报告基因定量检测鸡Ⅰ型干扰素活性性的研究[J]畜牧兽医学报,2008,39(1)：123-128
[88]夏春,万建青,吴志光,等.北京鸭Ⅰ型干扰素基因分子克隆与序列分析[J].畜牧兽医学报,2000,31(6)：567-570
[89]黄爱龙,Allison J,Ieva K鸭子α-干扰素基因表达及多样性分析[J].中国免疫学杂志,2000,16(12)：644-647
[90]王春霞,王林川,黄爱芳,等.番鸭IFN2 a基因的克隆与序列分析[J].华南农业大学学报,2002,31(6)：68-70
[91]龚永强,程安春,等.鸭干扰素成熟片段基因的原核表达及其表达产物抗鸭瘟强毒活性检测[J].中国兽医杂志.2008.44(7)：15-17
[92]Hong-Tao Li,Bo Ma,Jing-Wei Mi et al.Clong,in vitro expression and bioactivity of goose interferon-a[J].Cytokine,2006,34:177-183
[93]刘菲,赵婷婷,等.天府肉鹅IFN-a基因的克隆及生物信学分析[J].中国兽医科学,2010,40(12)：1271-1275
[94]刘菲,唐卫杰,等.鹅干扰素基因在COS-7细胞中的瞬时表达及抗病毒功能初探[J].浙江大学学报(农业与生命科学版)2011.37(4)：380-386
[95]Lillehoj H S,Kaspers B,Jenkins M C et al,Avian interferon and interleukin-2 review by comparison with mammalian homologues[J].Poultry Science Review,1992,4:67-85
[96]Lowenthal J W,Digby M R,York J J.Production of interferon-gamma by chicken T cells[J].J Interferon Cytokine Res,1995,15(11):933-938
[97]Yeh H Y,Winslow B J,Junker D E et al.In vitro effects of recombinant chicken interferon-gamma on immune cells[J].J Interferon Cytokine Res,1999,19(6):687-691
[98]Digby M R,Lowenthal J W,Cloning and expression of the chicken interferon-gamma gene[J].J Interferon Cytokine Res,1995,15(11):939-945
[99]Kaiser P,Sonnermans D,Smith L M,Avian IFN-gamma genes:sequence analysis suggests probable cross-species reactivity among galliforms[J].J Interferon Cytokine Res,1998,18(9):711-719
[100]刘胜旺,陈洪岩,孔宪刚,等.鸡γ-干扰素基因的分子克隆与序列测定[J].中国兽医学报,2000,20(3)：228-230
[101]吴志光,夏春,汪明.北京鸭Ⅱ型干扰素基因分子克隆与序列分析[J].中国兽医科技,2001,31(3)：7-9
[102]秘晶玮,王君伟,许丽娜,等.鹅α和γ干扰素基因的克隆及序列分析[J].中国预防兽医学报,2005,27(5)：359-362
[103]Li H T,Ma B,Mi J W,et al.Molecular cloning and functional analysis of goose interferon gamma[J].Vet Immunol Immunopathol,2007,117(2):67-74
[104]殷震,刘景华.动物病毒学.第二版[M].北京：科学出版社,1997,1165-1168
[105]Brown K E,Green S W,Young N S,Goose parvovirus An autonoumous member of the dependovirus genus[J] Virology,1995,210:283-291
[106]ZadoriZadori Z,Stefancsik R,Rauch T,et al.Analysis of the complete nucleotide sequence of goose parvovirus and uscovy duck parvovirus indicates common ancestral origin with adeno-assiciated virus [J]. Virology,1995,212:562-573
[107]Cater B J,Mendelson E,Trempe J P,AAV DNA replication integration and genetics in "Hand booke of Parvovirus"[M].CRC press,BOCA Raton F L.1169-226
[108]Christensen J,Cotmore S F,Tattersall P.Parvovirus initiation factor PIF:a novel human DNA-binding factor which coordinately recognizes two ACGT motifs[J].Viro,1997,71(8):5733-5741
[109]Brister R J,M uzyczka N.Rep-mediated nickling of Adeno-associated virus origin requires to biochemical activities,DNA helices activity and trans-interaction[J].J Virol,1999,73:9325-9336
[110]郝虹,潘惟钧.小鹅瘟病毒核酸链型与结构蛋白分析[J].病毒学报,1988,4(1)：28-32
[111]程由铨,林天龙,胡奇林,等.雏番鸭细小病毒的病原分离和鉴定[J].病毒学报,1993,9(3)：228-235
[112]Vihinen-Ranta M, Wang D, Weichert W S, et al.The VP1 N-terminal sequence of canine parvovirus affects nuclear transport of capsids efficient cell infection[J].Virol,2002,76:1884-1891
[113]Tullis G E,Burger L R,Pintel D J.The minor capid protein VP1 of the autonomous parvovirus minute virus of mice is dispensable for encapsidation of progeny single-stranded DNA but is required for infectivity. [J]Virol,1993,67:131-141
[114]Dumas B,Jourdan M,Pascaud A,et al. Complete nucleotide sequence of the cloned infections genome of Junonia Coenia densovirus reveals an organization unique among parvovirus[J].Virology,1992,191: 202-222
[115]Strassheim M L,Gruenberg A,Veijalainen P,et al,Two dominant neutralizing antigenic determinants of canine parvovirus are found on the threefold spike of the virus capsid[J]. Virology,1994,198(1):175-184
[116]Langeveld J P,Casal J I, Vela C.B-cell epitopes of canine parvovirus:distribution on the primary structure and exposure on the viral surface[J].J Virol,1993,67:765-772
[117]Le Call-Recule G,Jestin V,Chagnaug P,et al.Expression of Muscovy duck parvovirus capsid proteins(VP2 and VP3)in a baculovirus expression system:a demonstration of immunity induced by the recombinant proteins[J].J Gen Virol,1993,77:2159-2163
[118]李新华.抗小鹅瘟病毒中和性单克隆抗体的研制及实验防治效果[J].中国畜禽传染病,1998,4：247-24
[119]余兵,王永坤,刘宝荣,等.鹅细小病毒主要免疫原性蛋白基因的克隆与序列分析[J].病毒学报,2002,18(3)：259-263
[120]Paul A,Deleu L,Nuesch J P,et al.Inhibition of parvovirus minute virus of mice replication by appetite involved in the oliggomerzation of nonstructural protein NS1 [J].J Virol,1997,71:7393-7403
[121]Legrand K,Pommelaera J,Caillet F.MVM NS2 protein expression is required with transformed cells[J].Virol,1993,195:149-155
[122]Takehara K, Nakata T, Takizawak A et al. Expression of goose parvovirus VP1 capsid protein by a baculo-virus expression system and establishment of fluorescent antibody test to diagnose parvovirus infection[J].Arch Virol,1999,144 (8):1639-1645
[123]李雪梅,章金刚,向华,等.鹅细小病毒和番鸭细小病毒核酸疫苗重组质粒的构建及表达[J].生物技术通讯,2002,13(6)：433-435
[124]布日额,王君伟,吴金花.等,鹅细小病毒VP1与VP3非重叠序列的克隆与原核表达[J].中国兽医杂,2003,39(10)：3-6
[125]贺云霞,王君伟,马广鹏,等.鹅细小病毒VP2基因原核及真核表达载体的构建[J].中国兽医科技,2003,33(5)：30-33
[126]田丽红,贾永清,王君伟,等.鹅细小病毒VP3基因重组禽痘病毒转移载体的构建[J].中国兽医科技,2002,32(9)：5-8
[127]邢明伟,王君伟,贺云霞.等,鹅细小病毒NS2基因的克隆和序列分析[J].中国预防兽医学报,2003,25(3)：195-197
[128]马波,于君伟,李洪涛.等,鹅细小病毒VP1基因的克隆及序列分析[J]中国兽医科技,2003,33(12)：7-10
[129]马波,于天飞等,鹅细小病毒vpl基因的原核表达及抗原性分析[J]中国兽医科学2010,40(2)：135-138
[130]李宝臣,齐岩,马波.等,鹅细小病毒不同毒株VP3基因的序列分析比较[J].中国预防兽医学报,2003,25(6)：430-433
[131]葛艳,尤永进,徐泉兴.等,小鹅瘟病毒GPV-YG株主要开放性阅读框架核苷酸序列分析[J.]上海农业学报2005,21(2)：11-15
[132]张鑫,李柏岁,徐佳.鹅细小病毒四平分离株VP3基因的克隆与序列分析[J].吉林农业大学学报,2006,28(4)：436-439
[133]侯秋莲,王静,刘胜旺.等,鹅细小病毒VP2基因片段在原核系统中的表达及多克隆抗体的制备[J]中国病毒学,2005(4)：383-387
[134]高光,张鑫,徐佳,等.鹅细小病毒VP3基因真核表达载体的构建及其在Vero细胞中表达[J]中国兽医学报,2010,30(3)：323-326
[135]Joekstra J,T Smit,Van Brakel C.Observations on the host range and contro of goose virus hepatitis[J].Avian Pathol,1973,2 (3):169-178
[136]陈伯伦,叶本衡,黎杰虹.小鹅瘟鸭胚化GD弱毒疫苗的研究[J].畜牧兽医学报,1985,16(11)：269-273
[137]周阳生,王永坤,方定一.用小鹅瘟疫苗接种母鹅后对其雏鹅天然被动免疫期的测定[J].中国兽医杂志,1985,11(10)：51-52
[138]鞠环宇,卫娜,尚绪增,等.鹅细小病毒野毒株VP3基因的原核表达及抗原性检测[J]中国兽医科学,2009：39(6)：492-497
[139]朱德康,黎敏,等.小鹅瘟病毒VP3真核表达质粒与弱毒疫苗诱导鹅体免疫应答的比较[J].中国农业科学2011,44(3)：595-603
[140]于天飞,马波,邢明伟,等.鹅细小病毒非结构蛋白和结构蛋白的二级结构及B细胞抗原表位预测[J].中国家禽,2007,29(7)：7-11
[141]WAN G C Y,SHIEH H K,SHIEN J H, et al. Expression of capsid prot eins and non2structural prot eins of waterfowl parvoviruses in Escherichi a coli and t heir use in serological assays[J]. A vian Pat hol,2005,34 (5):376-382
[142]李忠明,张金刚,向华,.等.鹅细小病毒国内分离株主要结构蛋白(VP2-VP3)基因的克隆和序列分析[J].动物科学与动物医学,2001,18(3)：31-34
[143]Palmwe G A,Tattersall P,Autonomous parvoviruses as gene transfer vehicles[J].Contrib Microbiol. 2000;4:178-202
[144]赵丽荣,等.鹅细小病毒重组禽痘病毒的构建与免疫原性研究[J].中国预防兽医学报,2003,12：10-13
[145]M.leroy,G Pire.Baise,et al.Expression of the interferon-alpha/beta-inducible bovine Mxl dynamin interferes with replication of rabies virus[J].Neurobiology of Disease.2006,(21):515-521
[146]李玉峰,吴静,李峰,等.重组鸡α-干扰素对新城疫病毒复制的影响[J].家禽科学,2006(07)：73-75
[147]李公美,胡静涛,李玉梅,等.吉林白鹅α干扰素在大肠杆菌中的表达及抗病毒活性检测[J]中国兽医杂志,2012,48(2)：21-24
[148]萨姆布鲁克.J拉塞尔著；黄培堂等译.分子克隆实验指南(上、下册)[M].三版.北京；科学出版社,2002,8
[149]刘菲天府肉鹅α干扰素基因克隆、表达及其生物学活性研究,四川农业大学,博士论文,2011
[150]叶伟成,张存,王一成,等.绍兴鸭α干扰素基因克隆、表达及活性测定[J].浙江农业学报,2005,17(3)：115-119
[151]韦琴,彭贵青,金梅林,等.鸡α干扰素基因的克隆、原核表达及抗病毒效果研究[J].生物工程学报,2006,22(5)：737-743
[152]刘菲,高丽芹,程安春,等.天府肉鹅IFN-a成熟肽基因的原核表达及抗病毒活性[J].四川农业大学学报,2011,29(2)：269-273
[153]王巍,孙仰峰,魏双施,等.鹅IFN-a基因的高效表达[J].中国兽医科学,2009,39,(07)：636-640
[154]屈艾,汪承润.干扰素及其研究进展[J].徐州大学学报,2002,20(2)：57-60
[155]Pei J,Sekellick M J,Marcus P I,et al.Chicken interferon type I inhibits infections bronchitis virus replication and assiociated respiratory illness[J].Journal of Interferon and Cytokine Reserch, 2001,21,(12):1071-1077
[156]Xia C,Liu J,Wu Z,et al.The interferon-a genes from three chicken lines and its effects on H9N2 influenza viruses[J].Animal Biotechnology,2004,15(1):77-88
[157]Anthony M,Bi ological assays for interferon [J]Journal of Immunological Methods,2002,261:21-36
[158]郑晓玲,刘艳芬,陈绍红,等.重组狮头鹅α干扰素的制备及其抗病毒活性[J].中国预防兽医学报,2005,27(5)：359-362
[159]Elewaut D,Kronenberg M,et al.Molecular biology of NK T cell specificity and development[J].Semin Immunol.12(6):561-568;Schindler H et al.2001
[160]鸡γ干扰素成熟蛋白基因的表达及其产物抗病毒活性测定[J].中国病毒学,2004,19(1)：32-35
[161]胡晓静,刘淇,付薇,等.鹅γ干扰素原核表达及抗病毒活性研究[J].畜牧与兽医,2012,44(1)：65-69
[162]丁庆忠.鸡γ干扰素基因的克隆和表达及其多克隆抗体的制备[D].沈阳：东北农业大学,2003
[163]程坚,刘秀梵,彭大新,等.共表达H9亚型禽流行性感冒病毒血凝素基因和鸡Ⅱ型干扰素基因的重组鸡痘病毒构建及其免疫力[J].病毒学报,2003,19(1)：52-55
[164]曹瑞兵,蔡梅红,陈德胜,等.猪γ干扰素双顺反子表达载体的构建及在大肠杆菌中的表达[J].中国兽医学报,2004,24,(1)：52-54
[165]李公美,邵洪泽,刘海军.鹅细小病毒野毒的分离及VP3基因的克隆及序列分析[J]湖北农业科学[J].2010,49(4)：791-793
[166]韩新锋.串联不同分子佐剂的小鹅瘟病毒VP3基因疫苗的构建.四川农业大学,2008,6
[167]彭大新,刘秀梵,程坚等.共表达MDV糖蛋白B和鸡γ-干扰素基因的重组鸡痘病毒的构建[J].微生物学报,2002,42(5)：11-615
[168]Rautenschlein S, Sharma JM, Winslow BJ, et al.1999. Embryo vaccination of turkeys against Newcastle disease infection with recombinant fowlpox virus constructs containing interferon as adjuvants[J]. Vaccine,18(5-6):426-433
[169]Jiang Y,Xiao S,Fang L,et al.DNA vaccines co-expression GP5 and M proteins of porcine reproductive and respiratory syndrome virus(PRRSV)display enhanced immunogenicity [J].Vaccine,2006,24(15): 2869-2879
[2]Lampson GP.Tytell AA.Nemes MM.et al.Purification and characterization of chick embryo interferon [J].Proc Soc Exp Biol Med.1963,112:468-78
[3]Gray P W.Peung D W.Pennica D.et al.Expression of human immune interferon cDNA in E.coli and monkey cells[J].Nature.1982.295:503-506
[4]Dijkmans R,Vandenbroeck K,Beuker et al.Sequence of the porcine interferon-gamma gene[J].Nucleic Acids Research.1990.18:42-59
[5]李洪泉.干扰素抗病毒机制及其抗病毒性肝炎中的作用[J].中国公共卫生,2005,7(21)：890-891
[6]Knapp S,Yee LJ,Frodsham AJ,et al.Polymorphisms in interferon-induced genes and the outcome of hepatitis C virus infection:roles of MxA, OAS21 and PKR[J]. Genes Immun,2003,4 (6):411-419
[7]Uka K,Aikata H,Mori N,et al.Combination therapy of oral fluoropyrimidine anticancer drugS-1 and interferon alpha for HCC patients with extra hepatic metastases[J]Oncology,2008,75 (1-2):8-16
[8]Billiau A. Interferon-gamma:biology and role in pathogenesis[J]Adv Immunol,1996,62:61-130
[9]潘烨,郑起.干扰素在肿瘤治疗中应用的研究进展[J]国外医学外科学分,2005,32(1)：10-13
[10]Avendano S.C.Interferons:types and actions.Gastroenterol[J]Hepatol.2006.29 Suppl.2:125-128
[11]Lefevre.E.Interferon-delta the first member of a novel type interferon family[J].Biochimic 1998,80 (8-9):779-788
[12]Ishida N,Oritani K,Shiraga M et al. δDifferential effects of a novel[FN-ζlimitin and IFN-α on signals for Daxx induction and Crk phosphorylation that coup le with growth control of megakaryocytes [J].Experimental Hematology,2005,33:495-503
[13]Sheppard P, Kindsvogel W,Xu W,et al. IL-28,IL-29 and theirclass Ⅱ cytokine receptor IL-28R[J] Nat Immunol,2003,4 (1),:63-8
[14]曲建惠,张玲霞.干扰素的分子生物学机制研究进展[J].国际流行病学传染病学杂志,2006,33(1)：38-44
[15]Cheng G.,Chen W.Z.,Li Z.F,et al. Characterization of the Porcine alpha interferon multigene family[J] Gene.2006,382:28-38
[16]de Avila Botton S.,Brum M.C.S, Bautista E.et al. ImmunoPoteniiation of a foot-and-mouth disease virus subunit vaccine by interferon alpha [J].Vaccine 2006,24:3446-3456
[17]Golding,S.M.,Hedger R.S.,Talbot P.Radial immuno-diffusion and serum-neutralisation techniques for the assay of antibodies to swine vesicular disease[J]Res.Vet.Sci.1976-20:142-147
[18]孙亚萍,王英明,乔守怡.干扰素及其最新研究进展[J]中国免疫学杂志.2006,22(7)：676-679
[19]LaFleur D W, Nardelli B,Tsareva T et al. Interferon-k a novel type I interferon expressed in human keratinocytes [J]. J Biol Chem,2001,276:39765-39771
[20]Sun,T.Lu P.,Wang X.Localization of infection-related epitopes on the non-structural protein 3ABC of foot-and-mouth disease virus and the application of tandem epitopes[J].Virol.Methods,2004,119:79-86
[21]Kang,Y.M,Jin H. Xie Q.F. et al.The adjuvant effect of levamisole on Killed viral vaccines[J]. Vaccine,200523:5543-5550
[22]Langer JA,Cuortne EC,Kotenko S.The class II Cytokine receptor(CRF2)family:overview and patterns of receptor-ligand interactions[J].cytokine and growth factor reviews,2004,15:33-48
[23]韩春来.鸡干扰素与干扰素受体重组蛋白的生物学特性及其相互作用.中国农业大学博士论文,2005
[24]张纪岩.细胞因子受体超家族的结构保守性及生物学意义[J].国外医学分子生物学分册,1997,19(5)：193-196
[25]Lutfalla G, Gardiner K, Proudhon D et al The structure of the human interferon α/βreceptor gene[J].BiolChem,1992,276 (4):2802-2809
[26]Walter M R,Windsor WT, Nagabhushan TL et al.Crystal structure of a complex between interferon-yand its soluble high-affinity receptor [J].Natuer,1995,376 (20):230-35
[27]Kenji Oritani,Paul W Kincade,Cai Zhang,et al.Type I interferons and limitin:a comparison of structure,receptors and functions[J].Cytokine Growth Factor Rev.2001,12:337
[28]Lamken P.Lata S.Gavutis M et al.Ligand-induced assembling of the type I interferon receptor on supported lipid bilayers[J]Mol Biol.2004.341:303-318
[29]Langer J A,Yang J,Carmillo P et al.Bovine type I interferon protein BoIFNARl has high affinity and broad specifity for human type I interferons[J].FEBS Letters,1998,421:131-135
[30]Rhee S,Ebenspegrer C,Dembic Z et al.The structure of the gene for the second chain of the human interferon-yreceptor[J].Biol Chem,1996,271 (46):28947-28952
[31]Piehler J,Schreiber G. Fast transient cytokine-receptor interactions monitored in real time by reflectometric interference spectroscopy[J].Analytical B iochemistry,2001,289:173-186
[32]Kotenko S V. The fam ily of IL-10-related cytokines and their receptors:related, but to what extent [J]. Cytokine Growth Factor Rev,2002; 13:223-240
[33]Oritani K,Kincade PW,Zhang C et al.Type I interferons and limitin:a comparison of structures.receptors, and functions[J].Cytokine growth factor rev,2001,12:337-348
[34]Ragimbeau J,Dondi E,Alcover A et al.The tyrosine Tyk2 controls IFNAR1 cell surface expression [J]. EMBO J,2003,22(3):537-547
[35]Kumar KG,Krolewski JJ,Fuchs SY et al.Phosphorylation and specific ubiquitin-acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor[J].J Biol Chem,2004,5279(45):46614-20
[36]MuKamoto M,Kodama H,Baba T.Effects of cytokines from thymocytes and thymic stromal cells on chicken intrathymic T cell development[J].Veterinary Immunol Immunopathol,1999,67(3):223-33
[37]Pernis A,Gupta S,Gollob KJ et al.Lack of interferon-γ receptorβchain and the prevention of interferon ysignaling in Thl cells[J].Science,1995,269(5221):245-7
[38]Groux H,Sornasse T,Cottrez F et al.Induction of human T helper cell type I differentiation results in loss of IFN-y receptor (3 chain expression[J].The journal of Immunology,1997(158):5627-5631
[39]Rigamonti L,Aritti S,Losana G et al.Surface expression of the IFN-y R2 chains is regulated by intracellular trafficking in human T lymphocytes[J]. J Immunol,2000,164(1):201-7
[40]Skrenta H,Yang Y,Pestka S et al.Ligand-indepent down-regulation of IFN-y receptor 1 following TCR engagement[J].The journal of Immunology,2000,164(7):3506-11
[41]Bernabei P,Bosticardo M,Losana G et al.IGF-1 down-regulates IFN-y R2 chain surface expression and desensitizes IFN-y/STAT-1 signaling in human T lymphocytes[J].Blood,2003,102(8):2933-2939
[42]Novelli F,DElios MM,Bernabei P,et al.Expression and role in apoptosis of the alpha-and-beta-chains of the IFN-gamma receptor on human Th 1 and Th2 clones[J].The joural of Immunology,1997(159):306-213
[43]Altare F,Jouanguy E,Newport M et al.IFNGR1,a human mycobacterial susceptibility candidate gene[J].Bull Inst Pasteur,1997,95:143-146
[44]Zhang Ping,Chen Zhi,Chen Feng.et al Expression of IFN-gamma and its receptor alpha in the peripheral blood of patients with chronic hepatitis C[J]Chin Med.2004,117(1)79-82
[45]Osterlund PI,PietiIae TE, Veckman V, et al.IFN regulatory factor family members differentially regulate the expression of type Ⅲ IFN (IFN-λ.) genes.[J].J Immunol,2007,179 (6):3434-42
[46]Sadler AJ,Williams BR. Interferon-inducible antiviral effectors[J].Nat Rev Immunol,2008,8(7):559-68
[47]侯炜.Ⅲ型干扰素—新型抗病毒细胞因子[J].生命科学,2011,23,8：749-752
[48]Takaoka A.,Yanai H.Interferon signaling network in innate defense[J].Cell Microbiol,2006,8:907-922
[49]Haig DM,Poxvirus interference with the host cytokine respone[J].Vet Immunol,1998,63:149-156
[50]Leonard GT,Sen G C.Restoration of interferon response of adenovirus E1 A-expression HT1080 cell lines by overexpression of p48 protein.[J]. Virol,1997,71:5095-5101
[51]Elevy D,Adolfo G S. The virus battles:IFN induction of the antiviral state and mechanisms of viral evasion [J]. Cytokine and Growth Factor Reviews,2001, (12):143-156
[52]侯云德.分子病毒学.第二版.学苑出版社.北京：1990：201-212
[53]Samuel C.E.Antiviral actions of interferons.[J]Clin Microbiol Rev,2001,14:778-809
[54]Hall O M,G Kochs.Mx proteins:mediates of innate resistance to RNA virus[J].Rev Sci Technol Off Int Epizootol,1998,17:220-230
[55]王晓杜,猪干扰素α、β、γ基因克隆、原核表达及其单克隆抗体制备的研究.硕士毕业论文.华中农业大学,2007
[56]Tboullat D A,Hovanian GDavid, et al.Characterization of the gene coding the 100-kDa form of human 2'-5'oligoadenylate synthetas[J].Genomic,2000,70:232-240
[57]姚清侠.猪α干扰素、猪β干扰素与猪γ干扰素的抗病毒活性及其免疫佐剂的研究.博士学位论文,华中农业大学,2007
[58]Batusic D.S.et al.Induction of Mx-2 in rat liver by toxic injury[J] J Hepatol.2004,40(3):446-53
[59]于剑光.人类疱疹病毒8型[J]人类疱疹病毒8[J].国外医学：病毒学分册,2001,04：100-104
[60]Tiefenbrun N,Melamed D,Levy N,et al. Alpha interferon suppresses the cyclin D3 and cdc25A genes,leading to a reversible golike arrest[J]. Mol Cell Biol,1996,16:3934-3944
[61]Shin EC,Ahn JM,Kim CH, et al. IFN-gamma induces cell death in human hepatoma cells through a TRA 1L/death recep tor-mediated apoptotic pathway [J]. Int J Cancer,2001,93 (2):262-268
[62]Yoshiji H, Noguchi R, Kuriyama S,et al. Combination of interferon and angiotensin-converting enzyme inhibitor, perindop ril, sup-presses liver carcinogenesis and angiogenesis in mice [J].Oncol Rep, 2005,13 (3):491-495
[63]Wu W Z, Sun H C, Shen Y F, et al. Interferon alpha 2a down-regulates VEGF expression through PI3 kinase and MAP kinase signaling pathways[J]. J Cancer Res Clin Oncol,2005,131 (3):169-178
[64]Suginoshita Y, Kakimi K, et al. Impairment of IFN-a production capacity in patients with hepatitis C virus and the risk of the development of hepatocellular carcinoma[J].World J Gastroenterol,2005,11 (46):7330-7334
[65]Tosi D, Valenti R, Cora A, et al. Role of cross-talk between IFN-alpha induced momocyte-derived dendritic cells and NK cells in priming CD8+ T cell responses against human tumor antigens[J].J Imm unol,2004,172 (9):5363-5370
[66]Radaeva S, Jaruga B, Hong F, et al. Interferon-alpha activatesmultip le STAT signals and down-regulates c-Met in primary human hepatocytes[J]. Gastroenterology,2002,122 (4):1020-1034
[67]Xu D, Erickson S,Szeps M,et al. Interferon down-regulates telomerase reverse transcriptase and telomerase activity in human malignant and nonmalignant hematopoietic cells [J].Blood,2000,96 (13): 4313-4318
[68]赵明军,闫若潜,韩记用,等.猪α干扰素研究进展[J].畜禽业：南方养猪,2008,225(1)：48-51
[69]Le Bon A.Tough D F,Links between innate and adaptive immunity via type I interferon[J].Curr Opin Immunol.2002,14(4):432-6
[71]Jiang C.L.et al.Analgesic effect of interferon-alpha via mu opioid receptor in the rat[J].Neurochem Int. 2000,36(3):193-6
[72]Levy D.E,Garcia-Sastre A.The virus battles:IFN induction of the antivirual state and mechanisms of viral evasion[J].Cytokine Growth Factor Rev.2001,12(2-3):143-56
[73]马良.干扰素与寄生虫感染[J].重庆医科大学学报,1995,20(3)：241-244
[74]Min W,Lillehoj HS,Buxnside J,et al.Adjuvant effects of IL-1β, IL-2, IL-8, IL 15, IFN-a, IFN-γ, TGF-β4 and lymphotactin on DNA vaccination against Eimeria acervulina [J].Vaccine,2001,20 (1-2):267-274
[75]李延清.孙志宏.胡格.γ-干扰素与免疫-神经-内分泌网络的相互关系[J].延安大学学报(自然科学版).2003,22(3)：74-76
[76]徐天乐.张远强.细胞因子对内分泌的影响[J].国外医学·内分泌学分册.1992.12(3)：113-116
[77]Kato A,Kiyotani K,Kubota T,et al.Importance of the anti-interferon capacity of Sendai virus C protein for pathogenicity in mice[J].J Virol,2007,81(7):3264-3271
[78]Shaw M L,Garcia-Sastre A,Palese P,et al.Nipah virus V and W protein have a common STAT1-binding domain yet inhibit STAT1 activation from the cytoplasmic and nuclear compartments,respectively[J].J Virol,2004,78(11):5633-5641
[79]Kopecky-Bromberg S A,Martinez-Sobrido L,Frieman M et al.Severe acute respiratory syndrome coronavirus open reading frame(ORF)3b,ORF 6,and nucleocapsid proteins function as interferon antagonists[J].J Virol,2007,81(2):548-557
[80]Reid S P,Leung L W,Hartman A L,et al/Ebola virus VP24 binds karyopherin alphal and blocks STAT1 nuclear accumulation[J].J Virol,2006,80(11):5156-5167
[81]Sekellick M J,Ferrandino A F,Hopkins D A et al.Chicken interferon gene:cloning,expression,and analysis[J],J Interferon Res,1994,14(2):71-79
[82]Schultz, Rinderle C. Sekellick M J, et al. Recombinant chicken interferon from Escherichia coli and transfected COS cells is biologically active [J]. Europe Journal of Biochemistry.1995.229 (1):73-76
[83]夏春,汪明,朱凌云,等.惠阳胡须鸡IFN-α基因克隆和序列分析畜牧兽医学报[J].2000,31(6),563-56
[84]吕英姿,毕英佐,曹永长,等.石歧杂鸡γ-干扰素基因的克隆与序列分析华南农业大学学报[J].2002,23(4)：61-63
[85]韩春来,汪明,张灿,等.惠阳胡须鸡IFN-α基因的克隆表达和重组蛋白的抗病毒活性圆二色性分析[J].中国兽医杂志,2006,42(11)：3-6
[86]戴华,郑佳玉,陈俊华等,鸡α干扰素基因的原核表达及其活性测定[J]中国预防兽医学报,2009,31,10：805-807
[87]陈伟业.葛金英,肇慧君.基于Mx启动子和荧光素酶报告基因定量检测鸡Ⅰ型干扰素活性性的研究[J]畜牧兽医学报,2008,39(1)：123-128
[88]夏春,万建青,吴志光,等.北京鸭Ⅰ型干扰素基因分子克隆与序列分析[J].畜牧兽医学报,2000,31(6)：567-570
[89]黄爱龙,Allison J,Ieva K鸭子α-干扰素基因表达及多样性分析[J].中国免疫学杂志,2000,16(12)：644-647
[90]王春霞,王林川,黄爱芳,等.番鸭IFN2 a基因的克隆与序列分析[J].华南农业大学学报,2002,31(6)：68-70
[91]龚永强,程安春,等.鸭干扰素成熟片段基因的原核表达及其表达产物抗鸭瘟强毒活性检测[J].中国兽医杂志.2008.44(7)：15-17
[92]Hong-Tao Li,Bo Ma,Jing-Wei Mi et al.Clong,in vitro expression and bioactivity of goose interferon-a[J].Cytokine,2006,34:177-183
[93]刘菲,赵婷婷,等.天府肉鹅IFN-a基因的克隆及生物信学分析[J].中国兽医科学,2010,40(12)：1271-1275
[94]刘菲,唐卫杰,等.鹅干扰素基因在COS-7细胞中的瞬时表达及抗病毒功能初探[J].浙江大学学报(农业与生命科学版)2011.37(4)：380-386
[95]Lillehoj H S,Kaspers B,Jenkins M C et al,Avian interferon and interleukin-2 review by comparison with mammalian homologues[J].Poultry Science Review,1992,4:67-85
[96]Lowenthal J W,Digby M R,York J J.Production of interferon-gamma by chicken T cells[J].J Interferon Cytokine Res,1995,15(11):933-938
[97]Yeh H Y,Winslow B J,Junker D E et al.In vitro effects of recombinant chicken interferon-gamma on immune cells[J].J Interferon Cytokine Res,1999,19(6):687-691
[98]Digby M R,Lowenthal J W,Cloning and expression of the chicken interferon-gamma gene[J].J Interferon Cytokine Res,1995,15(11):939-945
[99]Kaiser P,Sonnermans D,Smith L M,Avian IFN-gamma genes:sequence analysis suggests probable cross-species reactivity among galliforms[J].J Interferon Cytokine Res,1998,18(9):711-719
[100]刘胜旺,陈洪岩,孔宪刚,等.鸡γ-干扰素基因的分子克隆与序列测定[J].中国兽医学报,2000,20(3)：228-230
[101]吴志光,夏春,汪明.北京鸭Ⅱ型干扰素基因分子克隆与序列分析[J].中国兽医科技,2001,31(3)：7-9
[102]秘晶玮,王君伟,许丽娜,等.鹅α和γ干扰素基因的克隆及序列分析[J].中国预防兽医学报,2005,27(5)：359-362
[103]Li H T,Ma B,Mi J W,et al.Molecular cloning and functional analysis of goose interferon gamma[J].Vet Immunol Immunopathol,2007,117(2):67-74
[104]殷震,刘景华.动物病毒学.第二版[M].北京：科学出版社,1997,1165-1168
[105]Brown K E,Green S W,Young N S,Goose parvovirus An autonoumous member of the dependovirus genus[J] Virology,1995,210:283-291
[106]ZadoriZadori Z,Stefancsik R,Rauch T,et al.Analysis of the complete nucleotide sequence of goose parvovirus and uscovy duck parvovirus indicates common ancestral origin with adeno-assiciated virus [J]. Virology,1995,212:562-573
[107]Cater B J,Mendelson E,Trempe J P,AAV DNA replication integration and genetics in "Hand booke of Parvovirus"[M].CRC press,BOCA Raton F L.1169-226
[108]Christensen J,Cotmore S F,Tattersall P.Parvovirus initiation factor PIF:a novel human DNA-binding factor which coordinately recognizes two ACGT motifs[J].Viro,1997,71(8):5733-5741
[109]Brister R J,M uzyczka N.Rep-mediated nickling of Adeno-associated virus origin requires to biochemical activities,DNA helices activity and trans-interaction[J].J Virol,1999,73:9325-9336
[110]郝虹,潘惟钧.小鹅瘟病毒核酸链型与结构蛋白分析[J].病毒学报,1988,4(1)：28-32
[111]程由铨,林天龙,胡奇林,等.雏番鸭细小病毒的病原分离和鉴定[J].病毒学报,1993,9(3)：228-235
[112]Vihinen-Ranta M, Wang D, Weichert W S, et al.The VP1 N-terminal sequence of canine parvovirus affects nuclear transport of capsids efficient cell infection[J].Virol,2002,76:1884-1891
[113]Tullis G E,Burger L R,Pintel D J.The minor capid protein VP1 of the autonomous parvovirus minute virus of mice is dispensable for encapsidation of progeny single-stranded DNA but is required for infectivity. [J]Virol,1993,67:131-141
[114]Dumas B,Jourdan M,Pascaud A,et al. Complete nucleotide sequence of the cloned infections genome of Junonia Coenia densovirus reveals an organization unique among parvovirus[J].Virology,1992,191: 202-222
[115]Strassheim M L,Gruenberg A,Veijalainen P,et al,Two dominant neutralizing antigenic determinants of canine parvovirus are found on the threefold spike of the virus capsid[J]. Virology,1994,198(1):175-184
[116]Langeveld J P,Casal J I, Vela C.B-cell epitopes of canine parvovirus:distribution on the primary structure and exposure on the viral surface[J].J Virol,1993,67:765-772
[117]Le Call-Recule G,Jestin V,Chagnaug P,et al.Expression of Muscovy duck parvovirus capsid proteins(VP2 and VP3)in a baculovirus expression system:a demonstration of immunity induced by the recombinant proteins[J].J Gen Virol,1993,77:2159-2163
[118]李新华.抗小鹅瘟病毒中和性单克隆抗体的研制及实验防治效果[J].中国畜禽传染病,1998,4：247-24
[119]余兵,王永坤,刘宝荣,等.鹅细小病毒主要免疫原性蛋白基因的克隆与序列分析[J].病毒学报,2002,18(3)：259-263
[120]Paul A,Deleu L,Nuesch J P,et al.Inhibition of parvovirus minute virus of mice replication by appetite involved in the oliggomerzation of nonstructural protein NS1 [J].J Virol,1997,71:7393-7403
[121]Legrand K,Pommelaera J,Caillet F.MVM NS2 protein expression is required with transformed cells[J].Virol,1993,195:149-155
[122]Takehara K, Nakata T, Takizawak A et al. Expression of goose parvovirus VP1 capsid protein by a baculo-virus expression system and establishment of fluorescent antibody test to diagnose parvovirus infection[J].Arch Virol,1999,144 (8):1639-1645
[123]李雪梅,章金刚,向华,等.鹅细小病毒和番鸭细小病毒核酸疫苗重组质粒的构建及表达[J].生物技术通讯,2002,13(6)：433-435
[124]布日额,王君伟,吴金花.等,鹅细小病毒VP1与VP3非重叠序列的克隆与原核表达[J].中国兽医杂,2003,39(10)：3-6
[125]贺云霞,王君伟,马广鹏,等.鹅细小病毒VP2基因原核及真核表达载体的构建[J].中国兽医科技,2003,33(5)：30-33
[126]田丽红,贾永清,王君伟,等.鹅细小病毒VP3基因重组禽痘病毒转移载体的构建[J].中国兽医科技,2002,32(9)：5-8
[127]邢明伟,王君伟,贺云霞.等,鹅细小病毒NS2基因的克隆和序列分析[J].中国预防兽医学报,2003,25(3)：195-197
[128]马波,于君伟,李洪涛.等,鹅细小病毒VP1基因的克隆及序列分析[J]中国兽医科技,2003,33(12)：7-10
[129]马波,于天飞等,鹅细小病毒vpl基因的原核表达及抗原性分析[J]中国兽医科学2010,40(2)：135-138
[130]李宝臣,齐岩,马波.等,鹅细小病毒不同毒株VP3基因的序列分析比较[J].中国预防兽医学报,2003,25(6)：430-433
[131]葛艳,尤永进,徐泉兴.等,小鹅瘟病毒GPV-YG株主要开放性阅读框架核苷酸序列分析[J.]上海农业学报2005,21(2)：11-15
[132]张鑫,李柏岁,徐佳.鹅细小病毒四平分离株VP3基因的克隆与序列分析[J].吉林农业大学学报,2006,28(4)：436-439
[133]侯秋莲,王静,刘胜旺.等,鹅细小病毒VP2基因片段在原核系统中的表达及多克隆抗体的制备[J]中国病毒学,2005(4)：383-387
[134]高光,张鑫,徐佳,等.鹅细小病毒VP3基因真核表达载体的构建及其在Vero细胞中表达[J]中国兽医学报,2010,30(3)：323-326
[135]Joekstra J,T Smit,Van Brakel C.Observations on the host range and contro of goose virus hepatitis[J].Avian Pathol,1973,2 (3):169-178
[136]陈伯伦,叶本衡,黎杰虹.小鹅瘟鸭胚化GD弱毒疫苗的研究[J].畜牧兽医学报,1985,16(11)：269-273
[137]周阳生,王永坤,方定一.用小鹅瘟疫苗接种母鹅后对其雏鹅天然被动免疫期的测定[J].中国兽医杂志,1985,11(10)：51-52
[138]鞠环宇,卫娜,尚绪增,等.鹅细小病毒野毒株VP3基因的原核表达及抗原性检测[J]中国兽医科学,2009：39(6)：492-497
[139]朱德康,黎敏,等.小鹅瘟病毒VP3真核表达质粒与弱毒疫苗诱导鹅体免疫应答的比较[J].中国农业科学2011,44(3)：595-603
[140]于天飞,马波,邢明伟,等.鹅细小病毒非结构蛋白和结构蛋白的二级结构及B细胞抗原表位预测[J].中国家禽,2007,29(7)：7-11
[141]WAN G C Y,SHIEH H K,SHIEN J H, et al. Expression of capsid prot eins and non2structural prot eins of waterfowl parvoviruses in Escherichi a coli and t heir use in serological assays[J]. A vian Pat hol,2005,34 (5):376-382
[142]李忠明,张金刚,向华,.等.鹅细小病毒国内分离株主要结构蛋白(VP2-VP3)基因的克隆和序列分析[J].动物科学与动物医学,2001,18(3)：31-34
[143]Palmwe G A,Tattersall P,Autonomous parvoviruses as gene transfer vehicles[J].Contrib Microbiol. 2000;4:178-202
[144]赵丽荣,等.鹅细小病毒重组禽痘病毒的构建与免疫原性研究[J].中国预防兽医学报,2003,12：10-13
[145]M.leroy,G Pire.Baise,et al.Expression of the interferon-alpha/beta-inducible bovine Mxl dynamin interferes with replication of rabies virus[J].Neurobiology of Disease.2006,(21):515-521
[146]李玉峰,吴静,李峰,等.重组鸡α-干扰素对新城疫病毒复制的影响[J].家禽科学,2006(07)：73-75
[147]李公美,胡静涛,李玉梅,等.吉林白鹅α干扰素在大肠杆菌中的表达及抗病毒活性检测[J]中国兽医杂志,2012,48(2)：21-24
[148]萨姆布鲁克.J拉塞尔著；黄培堂等译.分子克隆实验指南(上、下册)[M].三版.北京；科学出版社,2002,8
[149]刘菲天府肉鹅α干扰素基因克隆、表达及其生物学活性研究,四川农业大学,博士论文,2011
[150]叶伟成,张存,王一成,等.绍兴鸭α干扰素基因克隆、表达及活性测定[J].浙江农业学报,2005,17(3)：115-119
[151]韦琴,彭贵青,金梅林,等.鸡α干扰素基因的克隆、原核表达及抗病毒效果研究[J].生物工程学报,2006,22(5)：737-743
[152]刘菲,高丽芹,程安春,等.天府肉鹅IFN-a成熟肽基因的原核表达及抗病毒活性[J].四川农业大学学报,2011,29(2)：269-273
[153]王巍,孙仰峰,魏双施,等.鹅IFN-a基因的高效表达[J].中国兽医科学,2009,39,(07)：636-640
[154]屈艾,汪承润.干扰素及其研究进展[J].徐州大学学报,2002,20(2)：57-60
[155]Pei J,Sekellick M J,Marcus P I,et al.Chicken interferon type I inhibits infections bronchitis virus replication and assiociated respiratory illness[J].Journal of Interferon and Cytokine Reserch, 2001,21,(12):1071-1077
[156]Xia C,Liu J,Wu Z,et al.The interferon-a genes from three chicken lines and its effects on H9N2 influenza viruses[J].Animal Biotechnology,2004,15(1):77-88
[157]Anthony M,Bi ological assays for interferon [J]Journal of Immunological Methods,2002,261:21-36
[158]郑晓玲,刘艳芬,陈绍红,等.重组狮头鹅α干扰素的制备及其抗病毒活性[J].中国预防兽医学报,2005,27(5)：359-362
[159]Elewaut D,Kronenberg M,et al.Molecular biology of NK T cell specificity and development[J].Semin Immunol.12(6):561-568;Schindler H et al.2001
[160]鸡γ干扰素成熟蛋白基因的表达及其产物抗病毒活性测定[J].中国病毒学,2004,19(1)：32-35
[161]胡晓静,刘淇,付薇,等.鹅γ干扰素原核表达及抗病毒活性研究[J].畜牧与兽医,2012,44(1)：65-69
[162]丁庆忠.鸡γ干扰素基因的克隆和表达及其多克隆抗体的制备[D].沈阳：东北农业大学,2003
[163]程坚,刘秀梵,彭大新,等.共表达H9亚型禽流行性感冒病毒血凝素基因和鸡Ⅱ型干扰素基因的重组鸡痘病毒构建及其免疫力[J].病毒学报,2003,19(1)：52-55
[164]曹瑞兵,蔡梅红,陈德胜,等.猪γ干扰素双顺反子表达载体的构建及在大肠杆菌中的表达[J].中国兽医学报,2004,24,(1)：52-54
[165]李公美,邵洪泽,刘海军.鹅细小病毒野毒的分离及VP3基因的克隆及序列分析[J]湖北农业科学[J].2010,49(4)：791-793
[166]韩新锋.串联不同分子佐剂的小鹅瘟病毒VP3基因疫苗的构建.四川农业大学,2008,6
[167]彭大新,刘秀梵,程坚等.共表达MDV糖蛋白B和鸡γ-干扰素基因的重组鸡痘病毒的构建[J].微生物学报,2002,42(5)：11-615
[168]Rautenschlein S, Sharma JM, Winslow BJ, et al.1999. Embryo vaccination of turkeys against Newcastle disease infection with recombinant fowlpox virus constructs containing interferon as adjuvants[J]. Vaccine,18(5-6):426-433
[169]Jiang Y,Xiao S,Fang L,et al.DNA vaccines co-expression GP5 and M proteins of porcine reproductive and respiratory syndrome virus(PRRSV)display enhanced immunogenicity [J].Vaccine,2006,24(15): 2869-2879
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