浅刺针法对脑梗塞神经细胞保护机制的研究
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摘要
目的:研究浅刺针法对脑梗塞大鼠脑组织缺血半暗带神经细胞的保护作用及其机制。
方法:将72只SD大鼠随机分为浅刺针法组(Ⅰ组)、常规针刺组(Ⅱ组)、模型对照组(Ⅲ组)、正常对照组(Ⅳ组)。前三组采用血栓栓塞法建立脑梗塞大鼠模型。Ⅳ组不加任何处理因素,但在同一时间进行捆绑固定。每组再随机分为术后2、7、14天三个时间点,每个时间点各6只大鼠。从神经体征评分、HE染色观察脑组织的病理改变、采用免疫组化法测定脑组织Caspase-3、NGF、bFGF蛋白表达的变化等诸方面,观察经过2、7、14天治疗后的Ⅰ、Ⅱ组大鼠和相应时间未经治疗的Ⅲ、Ⅳ组大鼠大脑神经细胞增殖、再生及凋亡情况。从大脑神经功能重建、现代免疫学、分子生物学的角度揭示浅刺针法对脑梗塞大鼠脑组织缺血半暗带神经细胞的保护作用及其机制,为弘扬浅刺针法提供临床治疗脑梗塞疾病的手段及提高临床疗效均具有一定的意义。
结果:
1.浅刺针法可有效改善脑梗塞大鼠的神经缺损体征。
2.三个时间点的浅刺针法组与常规针刺组大鼠脑组织缺血半暗带的Caspase-3蛋白表达均明显低于模型组。
3.三个时间点的浅刺针法组与常规针刺组大鼠脑组织缺血半暗带的NGF、bFGF表达均明显高于模型组。
4.三个时间点的浅刺针法组大鼠脑组织缺血半暗带的Caspase-3蛋白表达又明显低于常规针刺组,NGF、bFGF表达又明显高于常规针刺组。
5.针刺后上调脑梗塞大鼠缺血半暗带NGF、bFGF的表达,可从阻止细胞内钙离子超载,减少自由基的损伤,抗兴奋性氨基酸的神经毒性等多方面干预凋亡产生的机制,从而下调caspase-3的表达,阻止caspase-3的活化,抑制神经细胞凋亡的发生,并促进脑损伤区神经细胞的增殖和再生。
结论:本研究显示了针刺对脑梗塞大鼠脑组织缺血半暗带神经细胞保护作用的机制可能与上调脑缺血损伤后NGF、bFGF表达水平,延长其高表达时程,抑制Caspase-3启动的神经元凋亡有关。而浅刺针法组上调NGF、bFGF表达,抑制Caspase-3表达的作用均优于常规针刺组。揭示经络在体表有其物质基础,浅刺可取得有效的刺激作用,使浅部组织的经络特异性能充分显示出来。
Objective:To study protective mechanisms of superficial needling to ischemic penumbra neuron in cerebral infarction rats.
Methods:72 SD rats were randomly divided into superficial needling group(GroupⅠ),routine needling group(GroupⅡ),model control group (GroupⅢ),normal control group(GroupⅢ).The first three groups were established as the cerebral infarction models with thromboembolism. GroupⅣwas tied up and fixed at the same time without any processing factors.Each group was divided again at postoperative 2days,7days 14 days three time points,each time points 6 rats.Observe the proliferation, regeneration and apoptosis with the rat brain neuron after 2,7,14 days in the treated GroupⅠ,Ⅱand the untreated groupⅢ,Ⅳin the corresponding time from the neurological score,brain pathological changes determined by HE staining,Caspaase-3,bFGF,NGF protein expression changes in brain tissue measured by immunohistochemistry method.It has remarkable social and practical significance to reveal the protective mechanisms of superficial needling to ischemic penumbra neuron in cerebral infarction rats in the view of cerebral nerve function reconstruction,modern immunology and molecular biology.
Result:
1.Superficial needling can effectively improve neurological defect sign in cerebral infarction rats.
2.Caspase-3 protein expression in brain tissue ischemic penumble rats of the superficial needling group and routine needling group were significantly lower than that of the model control group in the three time points.
3.NGF,bFGF expression in brain tissue ischemic penumble rats of superficial needling group and routine needling group were significantly higher than that of the model control group in the three time points.
4.Caspase-3 protein expression in brain tissue ischemic penumble rats of the superficial needling group was lower than that of routine needling group,while NGF,bFGF expression was higher than that of the routine needling group in the three time points.
5.Increasing blood NGF、bFGF expression level in ischemic penumbra neuron in cerebral infarction rats after superficial needling,can interfere the mechanism of apoptosis by preventing the overload of calcium ions, reducing free radical lesion and resisting excitatory amino acid toxicity, then reduce caspase-3 protein expression,prevent the activation of caspase-3,prohibit the apoptosis of neuron,and promote the proliferation and regeneration in injured zone.
Conclusion:The study confirmed that protective mechanisms of superficial needling to ischemic penumbra neuron might be related to increasing blood NGF,bFGF expression level after neurological defect, extending its high expression time-course,inhibiting the apoptosis of Caspase-3 activated neuron.Superficial needling group was superior to normal group in increasing NGF,bFGF expression,inhibiting Caspase-3 expression.It reveals that meridian has its material basis on the body surface and superficial needling has stimulating effect to make the shallow tissue show its meridian specificity.
方法:将72只SD大鼠随机分为浅刺针法组(Ⅰ组)、常规针刺组(Ⅱ组)、模型对照组(Ⅲ组)、正常对照组(Ⅳ组)。前三组采用血栓栓塞法建立脑梗塞大鼠模型。Ⅳ组不加任何处理因素,但在同一时间进行捆绑固定。每组再随机分为术后2、7、14天三个时间点,每个时间点各6只大鼠。从神经体征评分、HE染色观察脑组织的病理改变、采用免疫组化法测定脑组织Caspase-3、NGF、bFGF蛋白表达的变化等诸方面,观察经过2、7、14天治疗后的Ⅰ、Ⅱ组大鼠和相应时间未经治疗的Ⅲ、Ⅳ组大鼠大脑神经细胞增殖、再生及凋亡情况。从大脑神经功能重建、现代免疫学、分子生物学的角度揭示浅刺针法对脑梗塞大鼠脑组织缺血半暗带神经细胞的保护作用及其机制,为弘扬浅刺针法提供临床治疗脑梗塞疾病的手段及提高临床疗效均具有一定的意义。
结果:
1.浅刺针法可有效改善脑梗塞大鼠的神经缺损体征。
2.三个时间点的浅刺针法组与常规针刺组大鼠脑组织缺血半暗带的Caspase-3蛋白表达均明显低于模型组。
3.三个时间点的浅刺针法组与常规针刺组大鼠脑组织缺血半暗带的NGF、bFGF表达均明显高于模型组。
4.三个时间点的浅刺针法组大鼠脑组织缺血半暗带的Caspase-3蛋白表达又明显低于常规针刺组,NGF、bFGF表达又明显高于常规针刺组。
5.针刺后上调脑梗塞大鼠缺血半暗带NGF、bFGF的表达,可从阻止细胞内钙离子超载,减少自由基的损伤,抗兴奋性氨基酸的神经毒性等多方面干预凋亡产生的机制,从而下调caspase-3的表达,阻止caspase-3的活化,抑制神经细胞凋亡的发生,并促进脑损伤区神经细胞的增殖和再生。
结论:本研究显示了针刺对脑梗塞大鼠脑组织缺血半暗带神经细胞保护作用的机制可能与上调脑缺血损伤后NGF、bFGF表达水平,延长其高表达时程,抑制Caspase-3启动的神经元凋亡有关。而浅刺针法组上调NGF、bFGF表达,抑制Caspase-3表达的作用均优于常规针刺组。揭示经络在体表有其物质基础,浅刺可取得有效的刺激作用,使浅部组织的经络特异性能充分显示出来。
Objective:To study protective mechanisms of superficial needling to ischemic penumbra neuron in cerebral infarction rats.
Methods:72 SD rats were randomly divided into superficial needling group(GroupⅠ),routine needling group(GroupⅡ),model control group (GroupⅢ),normal control group(GroupⅢ).The first three groups were established as the cerebral infarction models with thromboembolism. GroupⅣwas tied up and fixed at the same time without any processing factors.Each group was divided again at postoperative 2days,7days 14 days three time points,each time points 6 rats.Observe the proliferation, regeneration and apoptosis with the rat brain neuron after 2,7,14 days in the treated GroupⅠ,Ⅱand the untreated groupⅢ,Ⅳin the corresponding time from the neurological score,brain pathological changes determined by HE staining,Caspaase-3,bFGF,NGF protein expression changes in brain tissue measured by immunohistochemistry method.It has remarkable social and practical significance to reveal the protective mechanisms of superficial needling to ischemic penumbra neuron in cerebral infarction rats in the view of cerebral nerve function reconstruction,modern immunology and molecular biology.
Result:
1.Superficial needling can effectively improve neurological defect sign in cerebral infarction rats.
2.Caspase-3 protein expression in brain tissue ischemic penumble rats of the superficial needling group and routine needling group were significantly lower than that of the model control group in the three time points.
3.NGF,bFGF expression in brain tissue ischemic penumble rats of superficial needling group and routine needling group were significantly higher than that of the model control group in the three time points.
4.Caspase-3 protein expression in brain tissue ischemic penumble rats of the superficial needling group was lower than that of routine needling group,while NGF,bFGF expression was higher than that of the routine needling group in the three time points.
5.Increasing blood NGF、bFGF expression level in ischemic penumbra neuron in cerebral infarction rats after superficial needling,can interfere the mechanism of apoptosis by preventing the overload of calcium ions, reducing free radical lesion and resisting excitatory amino acid toxicity, then reduce caspase-3 protein expression,prevent the activation of caspase-3,prohibit the apoptosis of neuron,and promote the proliferation and regeneration in injured zone.
Conclusion:The study confirmed that protective mechanisms of superficial needling to ischemic penumbra neuron might be related to increasing blood NGF,bFGF expression level after neurological defect, extending its high expression time-course,inhibiting the apoptosis of Caspase-3 activated neuron.Superficial needling group was superior to normal group in increasing NGF,bFGF expression,inhibiting Caspase-3 expression.It reveals that meridian has its material basis on the body surface and superficial needling has stimulating effect to make the shallow tissue show its meridian specificity.
引文
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