甘蓝型油菜千粒重及相关性状QTL定位
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摘要
千粒重及相关性状是重要的产量性状,对油菜产量的形成具有重要意义。本研究利用人工合成超大籽粒材料云南061009-1与两小籽粒材料07-5189与06-1007分别构建F2分离群体：F2A, F2B。对两F2分离群体进行表现鉴定、分子标记分析,进而定位控制千粒重、角果长、单位长度籽粒数三性状的QTL位点。主要结果如下：
1、大粒材料云南061009-1与小粒亲本07-5189及06-1007之间表现出极显著差异,适合于分离群体构建。
2、F2A与F2B群体表型数据进行统计分析并经正态检验,发现千粒重、角果长、单位长度籽粒数三性状在两群体中均符合正态分布,三性状均为多基因控制的数量性状,F2A与F2B群体适合于QTL分析。
3、F2A群体图谱包含93对SSR标记和128对AFLP标记,共有23个连锁群,全长2165.2 cM,标记间平均距离为10.62cM。以SSR标记作为铆钉标记,将F2A群体图谱与甘蓝型油菜通用图谱进行初步对应。
4、以127对SSR标记,52对AFLP标记,24对IP标记在F2B群体中的基因型数据构建包含23个连锁群全长1755.25cM的连锁图谱,标记间平均距离为9.73cM。利用共同的SSR标记,将F2B图谱与甘蓝型油菜通用图谱进行初步对应。
5、采用复合区间作图法,利用Win QTL Cartographer v2.5软件,分别在两群体中对千粒重、角果长、单位长度籽粒数进行QTL扫描。在F2A群体中检测到9个QTLs,在F2B群体中检测到7个QTLs, QTLs之间存在QTL簇现象,但未发现一因多效情况。
6、F2A群体QTL定位结果与F2B群体QTL定位结果进行比对,发现F2A群体中qaSW19-2、qaSW19-3与F2B群体中qbSW19位于两图谱的相同标记区间BnGMS405-BnGMS371内。
The 1000-seed weight and its related traits are important yield traits in Brassica napus which play important roles in seed yield. In this study we use a synthetic material Yunnan061009-1 the 1000-seed weight of which is super large and two small seed materials 07-5189 and 06-1007 to construct F2 segregating populations. Scan the QTLs associate with 3 traits,1000-seed, pod length and seed number per unit length. The main results are showed as follow:
1. Compare the parents of two population, it showed that 1000-seed weight, pod length, seed number per unit length are significant different between the large seed material Yunnan061009-1 and two small seed material 07-5189 and Hui 5900.
2. Through the phenotype data of F2A and F2B population and normal distribution test of all traits, it showed that 1000-seed weight, pod length, seed number per unit length are normally distribution.,so three traits are all quantitative trait, and F2A and F2B population is suitable for QTL analysis.
3. The genetic linkage map of F2A, was constructed with 93 simple sequence repeat (SSR) markers and 135 Amplified fragment length polymorphism (AFLP) markers, which span 1755.25 cM and the average interval distance is 9.50 cM. It has 23 linkage groups. Based on the SSR markers information, we align the map of F2A to the reference maps.
4. A linkage map with 127 simple sequence repeat (SSR) markers,52 amplified fragment length polymorphism (AFLP) markers and 24 intron polymorphism (IP) was contructed. It spans 1755.25 cM and has 23 linkage groups.The average interval distance of the linkage group is 9.50 cM. Based on the SSR markers information, we align the map of F2B to the reference maps.
5. By composite interval mapping method in Win QTL Cartographer v2.5 software, a total of 9 QTLs in F2A,7 QTLs in F2B associate with 1000-seed weight, pod length and seed number per unit lengthwere found. There is QTLs cluster, but no more effects gene in the result.
6. The QTLs qaSW19-2 and qaSW19-3 of F2A population and the QTL qbSW19 are in the same marker interval of BnGMS405-BnGMS371.
1、大粒材料云南061009-1与小粒亲本07-5189及06-1007之间表现出极显著差异,适合于分离群体构建。
2、F2A与F2B群体表型数据进行统计分析并经正态检验,发现千粒重、角果长、单位长度籽粒数三性状在两群体中均符合正态分布,三性状均为多基因控制的数量性状,F2A与F2B群体适合于QTL分析。
3、F2A群体图谱包含93对SSR标记和128对AFLP标记,共有23个连锁群,全长2165.2 cM,标记间平均距离为10.62cM。以SSR标记作为铆钉标记,将F2A群体图谱与甘蓝型油菜通用图谱进行初步对应。
4、以127对SSR标记,52对AFLP标记,24对IP标记在F2B群体中的基因型数据构建包含23个连锁群全长1755.25cM的连锁图谱,标记间平均距离为9.73cM。利用共同的SSR标记,将F2B图谱与甘蓝型油菜通用图谱进行初步对应。
5、采用复合区间作图法,利用Win QTL Cartographer v2.5软件,分别在两群体中对千粒重、角果长、单位长度籽粒数进行QTL扫描。在F2A群体中检测到9个QTLs,在F2B群体中检测到7个QTLs, QTLs之间存在QTL簇现象,但未发现一因多效情况。
6、F2A群体QTL定位结果与F2B群体QTL定位结果进行比对,发现F2A群体中qaSW19-2、qaSW19-3与F2B群体中qbSW19位于两图谱的相同标记区间BnGMS405-BnGMS371内。
The 1000-seed weight and its related traits are important yield traits in Brassica napus which play important roles in seed yield. In this study we use a synthetic material Yunnan061009-1 the 1000-seed weight of which is super large and two small seed materials 07-5189 and 06-1007 to construct F2 segregating populations. Scan the QTLs associate with 3 traits,1000-seed, pod length and seed number per unit length. The main results are showed as follow:
1. Compare the parents of two population, it showed that 1000-seed weight, pod length, seed number per unit length are significant different between the large seed material Yunnan061009-1 and two small seed material 07-5189 and Hui 5900.
2. Through the phenotype data of F2A and F2B population and normal distribution test of all traits, it showed that 1000-seed weight, pod length, seed number per unit length are normally distribution.,so three traits are all quantitative trait, and F2A and F2B population is suitable for QTL analysis.
3. The genetic linkage map of F2A, was constructed with 93 simple sequence repeat (SSR) markers and 135 Amplified fragment length polymorphism (AFLP) markers, which span 1755.25 cM and the average interval distance is 9.50 cM. It has 23 linkage groups. Based on the SSR markers information, we align the map of F2A to the reference maps.
4. A linkage map with 127 simple sequence repeat (SSR) markers,52 amplified fragment length polymorphism (AFLP) markers and 24 intron polymorphism (IP) was contructed. It spans 1755.25 cM and has 23 linkage groups.The average interval distance of the linkage group is 9.50 cM. Based on the SSR markers information, we align the map of F2B to the reference maps.
5. By composite interval mapping method in Win QTL Cartographer v2.5 software, a total of 9 QTLs in F2A,7 QTLs in F2B associate with 1000-seed weight, pod length and seed number per unit lengthwere found. There is QTLs cluster, but no more effects gene in the result.
6. The QTLs qaSW19-2 and qaSW19-3 of F2A population and the QTL qbSW19 are in the same marker interval of BnGMS405-BnGMS371.
引文
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