RNA干扰抑制Snail基因表达对于肝癌HepG2细胞上皮间质转化以及体外侵袭的影响
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摘要
目的：以RNA干扰（RNAinterference,RNAi）方法抑制转录因子Snail的表达后，研究对人肝癌HepG2细胞株上皮间质转化（epithelial-mesenchymal transition, EMT）的表型和体外侵袭能力的影响。
方法：设计并合成Snail的小干扰RNA（Snail siRNA）和阴性对照siRNA，分别转染肝癌HepG2细胞，得到Snail表达受抑制的Snail siRNA组细胞和Snail表达未受影响的Negative control组细胞。分别采用Western blot和RT-PCR技术检测Control组（正常非转染组）、Negative control组和Snail siRNA组细胞Snail、E-cadherin和Vimentin的表达，用Transwell小室模型检测细胞侵袭能力。
结果：Control组细胞：Snail和Vimentin表达较强，但是E-cadherin表达较弱。Snail siRNA组与Control组相比，Snail和Vimentin的表达显著减弱（P＜0.05），E-cadherin的表达显著增强（P＜0.05），Transwell小室透膜细胞数显著减少（P＜0.05）。Negative control组与Control组相比，Snail、E-cadherin和Vimentin的表达及Transwell小室透膜细胞数均无显著差异（P＞0.05）。
结论：通过RNA干扰抑制Snail表达能有效地抑制HepG2细胞上皮间质转化过程和体外侵袭能力。Snail可能在肝细胞癌上皮间质转化及侵袭过程中发挥重要作用，抑制Snail表达可能成为治疗肝细胞癌的可行方法。
Objective: To investigate the effect of RNA interference(RNAi) targetingtranscription factor Snail on epithelial-mesenchymal transition(EMT) phenotype andinvasion ability of human hepatocellular carcinoma HepG2cell in vitro.
Methods: The siRNA targeting Snail and negatvie control siRNA weredesigned,chemical synthesized and transfected into human hepatocellular carcinomaHepG2cell,Snail siRNA group cells expressing Snail suppressed and Negative controlgroup cells expressing Snail uninfluenced were obtained.In Control group(nontransfectiongroup),Negative control group and Snail siRNA group cells,Snail,E-cadherin and Vimentinexpression were detected by using Western blot and RT-PCR,invasion ability was detectedby using Transwell chamber model.
Results: Control group:Both Snail and Vimentin expression were strong positive,butE-cadherin expression was poor positive.Snail siRNA group compared with Controlgroup,Snail and Vimentin expression decreased significantly（P＜0.05）,E-cadherinexpression increased significantly（P＜0.05）,the numbers of cells permeating septum ofTranswell chamber decreased significantly（P＜0.05）.Negative control group comparedwith Control group,Snail、E-cadherin and Vimentin expression,and the numbers of cellspermeating septum of Transwell chamber were not significantly different（P＞0.05）.
Conclusion: RNA interference targeting Snail expression can inhibit efficientlyepithelial-mesenchymal transition and invasion ability of HepG2cells in vitro. Snailmight play a crucial role in epithelial-mesenchymal transition and invasion of hepatocellular carcinoma,and suppression of Snail expression might be a promisingstrategy for the treatment of human hepatocellular carcinoma.
方法：设计并合成Snail的小干扰RNA（Snail siRNA）和阴性对照siRNA，分别转染肝癌HepG2细胞，得到Snail表达受抑制的Snail siRNA组细胞和Snail表达未受影响的Negative control组细胞。分别采用Western blot和RT-PCR技术检测Control组（正常非转染组）、Negative control组和Snail siRNA组细胞Snail、E-cadherin和Vimentin的表达，用Transwell小室模型检测细胞侵袭能力。
结果：Control组细胞：Snail和Vimentin表达较强，但是E-cadherin表达较弱。Snail siRNA组与Control组相比，Snail和Vimentin的表达显著减弱（P＜0.05），E-cadherin的表达显著增强（P＜0.05），Transwell小室透膜细胞数显著减少（P＜0.05）。Negative control组与Control组相比，Snail、E-cadherin和Vimentin的表达及Transwell小室透膜细胞数均无显著差异（P＞0.05）。
结论：通过RNA干扰抑制Snail表达能有效地抑制HepG2细胞上皮间质转化过程和体外侵袭能力。Snail可能在肝细胞癌上皮间质转化及侵袭过程中发挥重要作用，抑制Snail表达可能成为治疗肝细胞癌的可行方法。
Objective: To investigate the effect of RNA interference(RNAi) targetingtranscription factor Snail on epithelial-mesenchymal transition(EMT) phenotype andinvasion ability of human hepatocellular carcinoma HepG2cell in vitro.
Methods: The siRNA targeting Snail and negatvie control siRNA weredesigned,chemical synthesized and transfected into human hepatocellular carcinomaHepG2cell,Snail siRNA group cells expressing Snail suppressed and Negative controlgroup cells expressing Snail uninfluenced were obtained.In Control group(nontransfectiongroup),Negative control group and Snail siRNA group cells,Snail,E-cadherin and Vimentinexpression were detected by using Western blot and RT-PCR,invasion ability was detectedby using Transwell chamber model.
Results: Control group:Both Snail and Vimentin expression were strong positive,butE-cadherin expression was poor positive.Snail siRNA group compared with Controlgroup,Snail and Vimentin expression decreased significantly（P＜0.05）,E-cadherinexpression increased significantly（P＜0.05）,the numbers of cells permeating septum ofTranswell chamber decreased significantly（P＜0.05）.Negative control group comparedwith Control group,Snail、E-cadherin and Vimentin expression,and the numbers of cellspermeating septum of Transwell chamber were not significantly different（P＞0.05）.
Conclusion: RNA interference targeting Snail expression can inhibit efficientlyepithelial-mesenchymal transition and invasion ability of HepG2cells in vitro. Snailmight play a crucial role in epithelial-mesenchymal transition and invasion of hepatocellular carcinoma,and suppression of Snail expression might be a promisingstrategy for the treatment of human hepatocellular carcinoma.
引文
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