加味熙春饮合玉女补乳酥对实验性卵巢早衰康复作用的实验研究
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摘要
目的：观察加味熙春饮合玉女补乳酥对实验性卵巢早衰大鼠的保健康复疗效,初步探讨该药食的作用机理,为卵巢早衰的预防保健及临床治疗提供新思路。
方法：用健康雌性SD大鼠,随机分为三组：空白组、药食组、模型组。模型组和药食组喂给35%浓度的D-半乳糖饲料造模。药食组饲料中按15%浓度配入花生、红枣、黄豆的混合物,另枸杞子、龙眼肉、女贞子、熟地黄、淫羊藿、补骨脂、覆盆子、菟丝子8味药物醇提后每天灌胃,以上处理均为1次/天,连续60天。于第61天采血前禁食12小时,眼眶静脉取血,离心后分离血清,采用放免法测定FSH、E2。大鼠采血后予颈椎脱臼处死,留取双侧卵巢于电子天平上称取重量,计算其卵巢指数。卵巢称重后置福尔马林固定,梯度酒精脱水,石蜡包埋,切片,常规HE染色,置光学显微镜下观察。
结果：(1)实验前各组大鼠体重P>0.05,无显著性差异。试验后,模型组大鼠体重与空白组及药食防治组相比P<0.05,差异有显著性；空白组与药食组相比P>0.05,无显著性差异。(2)模型组与空白组相比,FSH水平上升,E2水平下降,二者两组分别相比差异有显著性P<0.01,P<0.01；药食组与模型组相比FSH水平下降,E2水平升高,二者两组分别相比差异有显著性P<0.01,P<0.01；空白组与药食组相比,无显著性差异P>0.05。(3)空白组与药食组卵巢指数相比,P>0.05,无显著性差异；模型组分别与空白组和药食组相比,卵巢指数明显降低,均P<0.05,差异有显著性。(4)模型组卵巢组织形态观察有明显病理改变,药食组卵巢组织形态则接近于正常。
结论：实验中药食可通过调节下丘脑-垂体-卵巢轴的功能,改善卵巢功能,并发挥雌激素样作用,改善卵巢功能衰退引起的体重减轻、多汗等症状,调节卵巢对促性腺激素的反应性,促进卵泡发育。实验结果可为女性卵巢保健食品开发提供一定的理论依据。
Objective:To observe the rehabilitation of health effects by using the modified XiChunyin and YuNvburusu for premature ovarian failure in experimental rats, and discuss the mechanism of this mixed herb and food for premature ovarian failure to provide preventive health care and clinical treatment of new ideas.
Methods:Healthy female SD rats are divided into three groups, blank-control group, herb food group, model group. Model group and herb food group are fed by 35% concentration of D-galactose to make model. The food of herb food group are added 15% concentration of the mixture of peanuts, red dates, soy, and the other herb:gouqizi, longyanrou, nvzhenzi, shudihuang, yinyanghuo, buguzhi, fupengzi, tusizi are extracted by ethanol, then gavage the rats with this liquide every day. These handles are all done 1 times/day,60 days successively. After 60 successive days,all rats of three group are fasting by 12 hours before blood sample taken in orbital vein on the 61th day,then separate the blood serum after centrifugalization and detect the content of FSH、E2 with radioinununity method. The rats are put to death by cervieal uertebra disloeated after the sample taken in the eye ball. Then put their ovaries on the electronic balance and calculate the ovarian index and retain them in formalin, gradient alcoholic dehydration, paraffin imbedding, mierotome section, routine HE dyeing and then oberve it under the light microscope.
The results:(1) Weight of rats in each group before the experiment P>0.05, no significant difference. After the test, comparing the rats'weight of the model group with the blank-control group and the herb food group P<0.05, significant difference; the blank-control group is compared with the herb food P>0.05, no significant difference. (2) Comparing the model group to the blank-control group, FSH levels rise, E2 levels decreased, compared the two groups are significantly different P<0.01, P<0.01; comparing the herb food group to the model group, FSH levels decreased, E2 levels increased, the two groups were significantly different compared to P<0.01, P<0.01; the blank-control group's compared with the herb food group's, no significant difference P>0.05. (3) Comparing ovarian index of the herb food group's and the blank-controll group, P>0.05, no significant difference; the model group are compared with the blank-control group and the herb food group, its ovarian index was significantly lower, both P<0.05, significant difference. (4) The model group's tissue morphology of ovarian have obviously pathological changes, and the herb food group's were close to normal.
Conclusion:The mixture of the herb and food in experimental can adjust the function of the hypothalamus-pituitary-ovarian, improve ovarian's function and play a estrogen-like role to improve weight loss, hyperhidrosis and other symptoms which can caused by ovarian failure, regulating the ovarian's response to gonadotropin, promoting development of follicle. The results of experiment can provide a theoretical basis for the development of health food for the female ovaries.
方法：用健康雌性SD大鼠,随机分为三组：空白组、药食组、模型组。模型组和药食组喂给35%浓度的D-半乳糖饲料造模。药食组饲料中按15%浓度配入花生、红枣、黄豆的混合物,另枸杞子、龙眼肉、女贞子、熟地黄、淫羊藿、补骨脂、覆盆子、菟丝子8味药物醇提后每天灌胃,以上处理均为1次/天,连续60天。于第61天采血前禁食12小时,眼眶静脉取血,离心后分离血清,采用放免法测定FSH、E2。大鼠采血后予颈椎脱臼处死,留取双侧卵巢于电子天平上称取重量,计算其卵巢指数。卵巢称重后置福尔马林固定,梯度酒精脱水,石蜡包埋,切片,常规HE染色,置光学显微镜下观察。
结果：(1)实验前各组大鼠体重P>0.05,无显著性差异。试验后,模型组大鼠体重与空白组及药食防治组相比P<0.05,差异有显著性；空白组与药食组相比P>0.05,无显著性差异。(2)模型组与空白组相比,FSH水平上升,E2水平下降,二者两组分别相比差异有显著性P<0.01,P<0.01；药食组与模型组相比FSH水平下降,E2水平升高,二者两组分别相比差异有显著性P<0.01,P<0.01；空白组与药食组相比,无显著性差异P>0.05。(3)空白组与药食组卵巢指数相比,P>0.05,无显著性差异；模型组分别与空白组和药食组相比,卵巢指数明显降低,均P<0.05,差异有显著性。(4)模型组卵巢组织形态观察有明显病理改变,药食组卵巢组织形态则接近于正常。
结论：实验中药食可通过调节下丘脑-垂体-卵巢轴的功能,改善卵巢功能,并发挥雌激素样作用,改善卵巢功能衰退引起的体重减轻、多汗等症状,调节卵巢对促性腺激素的反应性,促进卵泡发育。实验结果可为女性卵巢保健食品开发提供一定的理论依据。
Objective:To observe the rehabilitation of health effects by using the modified XiChunyin and YuNvburusu for premature ovarian failure in experimental rats, and discuss the mechanism of this mixed herb and food for premature ovarian failure to provide preventive health care and clinical treatment of new ideas.
Methods:Healthy female SD rats are divided into three groups, blank-control group, herb food group, model group. Model group and herb food group are fed by 35% concentration of D-galactose to make model. The food of herb food group are added 15% concentration of the mixture of peanuts, red dates, soy, and the other herb:gouqizi, longyanrou, nvzhenzi, shudihuang, yinyanghuo, buguzhi, fupengzi, tusizi are extracted by ethanol, then gavage the rats with this liquide every day. These handles are all done 1 times/day,60 days successively. After 60 successive days,all rats of three group are fasting by 12 hours before blood sample taken in orbital vein on the 61th day,then separate the blood serum after centrifugalization and detect the content of FSH、E2 with radioinununity method. The rats are put to death by cervieal uertebra disloeated after the sample taken in the eye ball. Then put their ovaries on the electronic balance and calculate the ovarian index and retain them in formalin, gradient alcoholic dehydration, paraffin imbedding, mierotome section, routine HE dyeing and then oberve it under the light microscope.
The results:(1) Weight of rats in each group before the experiment P>0.05, no significant difference. After the test, comparing the rats'weight of the model group with the blank-control group and the herb food group P<0.05, significant difference; the blank-control group is compared with the herb food P>0.05, no significant difference. (2) Comparing the model group to the blank-control group, FSH levels rise, E2 levels decreased, compared the two groups are significantly different P<0.01, P<0.01; comparing the herb food group to the model group, FSH levels decreased, E2 levels increased, the two groups were significantly different compared to P<0.01, P<0.01; the blank-control group's compared with the herb food group's, no significant difference P>0.05. (3) Comparing ovarian index of the herb food group's and the blank-controll group, P>0.05, no significant difference; the model group are compared with the blank-control group and the herb food group, its ovarian index was significantly lower, both P<0.05, significant difference. (4) The model group's tissue morphology of ovarian have obviously pathological changes, and the herb food group's were close to normal.
Conclusion:The mixture of the herb and food in experimental can adjust the function of the hypothalamus-pituitary-ovarian, improve ovarian's function and play a estrogen-like role to improve weight loss, hyperhidrosis and other symptoms which can caused by ovarian failure, regulating the ovarian's response to gonadotropin, promoting development of follicle. The results of experiment can provide a theoretical basis for the development of health food for the female ovaries.
引文
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