MMP-9、LMP-1在鼻NK/T细胞淋巴瘤组织中的表达及意义
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摘要
目的:
通过检测鼻NK/T细胞淋巴瘤组织中的MMP-9(基质金属蛋白酶-9)、LMP-1(潜伏膜蛋白-1)的表达及分布情况,探讨两者在鼻NK/T细胞淋巴瘤发生,发展中的作用。
方法:
采用免疫组化EliVision二步法检测40例鼻NK/T细胞淋巴瘤组织中MMP-9、LMP-1的表达情况,同时与组织芯片中30例其他类型淋巴瘤进行比较研究,20例淋巴组织反应性增生作为对照。
结果:
鼻NK/T细胞淋巴瘤组中MMP-9、LMP-1阳性表达率分别为87.0%(35/40)和40.0%(16/40),组织芯片包含的B细胞淋巴瘤组及T细胞淋巴瘤组MMP-9的阳性表达率分别为61.1% (11/18)、50.0%(6/12),LMP-1的阳性表达率分别为11.1%(2/18)、25.0%(3/12)。淋巴组织反应性增生组有1例LMP-1的阳性表达, MMP-9的阳性表达率为40.0% (8/20)。鼻NK/T细胞淋巴瘤组MMP-9、LMP-1阳性表达率与淋巴组织反应性增生组比较差异有统计学意义(P<0.05),MMP-9、LMP-1的表达与鼻NK/T细胞淋巴瘤病人的年龄、性别、临床分期,LDH水平无关,MMP-9在鼻NK/T细胞淋巴瘤中的表达要高于T细胞淋巴瘤。在鼻NK/T细胞淋巴瘤中,MMP-9与LMP-1表达呈正相关(r=0.625, P<0.05)。
结论:
MMP-9在鼻NK/T细胞淋巴瘤组织中存在高表达, LMP-1能上调MMP-9表达,与EB病毒感染关系密切,LMP-1及MMP-9在鼻NK/T细胞淋巴瘤的发生发展中发挥了重要的作用。
Objective To detect the expression and distribution of matrix metalloproteinase - 9 and latent membrane protein-1 in nasal NK/T cell lymphoma,and to explore their roles in generation and development of nasal NK/T cell lymphoma .
Methods Immunohistochemistry by EliVision technique was used to detect the expression of matrix metalloproteinase-9 and latent membrane protein-1 in nasal NK/T cell lymphoma,and a tissue chip which consists of other type lymphoma were used for relative research,meanwhile 20 cases of reactive lymphoid hyperplasia were also used for comparison . Results The positive expression rate of MMP-9 and LMP-1 in the group of nasal NK/T cell lymphoma was 87.0%(35/40)and 40.0%(16/40)respectively. Tissue chip consists of the group of B-cell lymphoma and T-cell lymphoma .The positive expression rate of MMP-9 and LMP-1 in the group of B-cell lymphoma was 61.1%(11/18)and 11.1%(2/18)respectively ,while those in 12 cases of T-cell lymphoma was 50.0%(6/12)and 25.0%(3/12)respectively. There is only one case of positive expression of LMP-1 in the group of reactive lymphoid hyperplasia and the positive expression rate of MMP-9 was 40.0%(8/20)in it. The positive expression rates of MMP-9 and LMP-1 between the group of nasal NK/T cell lymphoma and reactive lymphoid hyperplasia had significant difference statistically(P<0.05). The expression of MMP-9 and LMP-1 have no correlation with the age, gender, clinical stage and the level of LDH of the patients who have been diagnosis with nasal NK/T cell lymphoma . The positive expression rate of MMP-9 in the group of nasal NK/T cell lymphoma was higher than T-cell lymphoma in tissue chip. The expression of MMP-9 showed a positive correlation with the expression of LMP-1 in nasal NK/T cell lymphoma.
Conclusion MMP-9 is highly expressed in nasal NK/T cell lymphoma. LMP-1 which is close correlated with the virus of EB can upregulate the expression of MMP-9. MMP-9 and LMP-1 may play a critical role in the generation and development of nasal NK/T cell lymphoma .
通过检测鼻NK/T细胞淋巴瘤组织中的MMP-9(基质金属蛋白酶-9)、LMP-1(潜伏膜蛋白-1)的表达及分布情况,探讨两者在鼻NK/T细胞淋巴瘤发生,发展中的作用。
方法:
采用免疫组化EliVision二步法检测40例鼻NK/T细胞淋巴瘤组织中MMP-9、LMP-1的表达情况,同时与组织芯片中30例其他类型淋巴瘤进行比较研究,20例淋巴组织反应性增生作为对照。
结果:
鼻NK/T细胞淋巴瘤组中MMP-9、LMP-1阳性表达率分别为87.0%(35/40)和40.0%(16/40),组织芯片包含的B细胞淋巴瘤组及T细胞淋巴瘤组MMP-9的阳性表达率分别为61.1% (11/18)、50.0%(6/12),LMP-1的阳性表达率分别为11.1%(2/18)、25.0%(3/12)。淋巴组织反应性增生组有1例LMP-1的阳性表达, MMP-9的阳性表达率为40.0% (8/20)。鼻NK/T细胞淋巴瘤组MMP-9、LMP-1阳性表达率与淋巴组织反应性增生组比较差异有统计学意义(P<0.05),MMP-9、LMP-1的表达与鼻NK/T细胞淋巴瘤病人的年龄、性别、临床分期,LDH水平无关,MMP-9在鼻NK/T细胞淋巴瘤中的表达要高于T细胞淋巴瘤。在鼻NK/T细胞淋巴瘤中,MMP-9与LMP-1表达呈正相关(r=0.625, P<0.05)。
结论:
MMP-9在鼻NK/T细胞淋巴瘤组织中存在高表达, LMP-1能上调MMP-9表达,与EB病毒感染关系密切,LMP-1及MMP-9在鼻NK/T细胞淋巴瘤的发生发展中发挥了重要的作用。
Objective To detect the expression and distribution of matrix metalloproteinase - 9 and latent membrane protein-1 in nasal NK/T cell lymphoma,and to explore their roles in generation and development of nasal NK/T cell lymphoma .
Methods Immunohistochemistry by EliVision technique was used to detect the expression of matrix metalloproteinase-9 and latent membrane protein-1 in nasal NK/T cell lymphoma,and a tissue chip which consists of other type lymphoma were used for relative research,meanwhile 20 cases of reactive lymphoid hyperplasia were also used for comparison . Results The positive expression rate of MMP-9 and LMP-1 in the group of nasal NK/T cell lymphoma was 87.0%(35/40)and 40.0%(16/40)respectively. Tissue chip consists of the group of B-cell lymphoma and T-cell lymphoma .The positive expression rate of MMP-9 and LMP-1 in the group of B-cell lymphoma was 61.1%(11/18)and 11.1%(2/18)respectively ,while those in 12 cases of T-cell lymphoma was 50.0%(6/12)and 25.0%(3/12)respectively. There is only one case of positive expression of LMP-1 in the group of reactive lymphoid hyperplasia and the positive expression rate of MMP-9 was 40.0%(8/20)in it. The positive expression rates of MMP-9 and LMP-1 between the group of nasal NK/T cell lymphoma and reactive lymphoid hyperplasia had significant difference statistically(P<0.05). The expression of MMP-9 and LMP-1 have no correlation with the age, gender, clinical stage and the level of LDH of the patients who have been diagnosis with nasal NK/T cell lymphoma . The positive expression rate of MMP-9 in the group of nasal NK/T cell lymphoma was higher than T-cell lymphoma in tissue chip. The expression of MMP-9 showed a positive correlation with the expression of LMP-1 in nasal NK/T cell lymphoma.
Conclusion MMP-9 is highly expressed in nasal NK/T cell lymphoma. LMP-1 which is close correlated with the virus of EB can upregulate the expression of MMP-9. MMP-9 and LMP-1 may play a critical role in the generation and development of nasal NK/T cell lymphoma .
引文
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