青春期酒精刺激对大鼠成年后空间学习记忆以及海马结构突触囊泡素表达的影响
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摘要
目的:探讨青春期酒精刺激对大鼠成年后学习记忆与海马结构突触囊泡素表达的影响
方法:取青春期40只SD大鼠分为对照组与实验组,两组动物分别以生理盐水、2g/kg酒精连续注射5天,每天固定时间2次,然后正常饲养,待成年后用Morris水迷宫对各组大鼠进行空间辨别性学习记忆的测试,用摄像机拍摄大鼠的行为,再用统计分析软件分析水迷宫检测结果。用免疫组织化学方法检测正常对照组与实验组大鼠海马结构突触囊泡素的表达。
结果:Morris水迷宫结果:各组大鼠随着训练次数的增加逃避潜伏期(EL)所用时间逐渐缩短。酒精组逃避潜伏期(EL)比正常对照组长,差异有统计学意义(P＜0.05)。
突触囊泡素免疫表达:突触囊泡素的免疫表达主要集中表达在海马结构的CA_3以及齿状回,为褐色颗粒状,呈板状分布。正常对照组与酒精组比较,大鼠海马结构CA_3跟齿状回突触囊泡素免疫反应阳性产物的平均灰度值差异有统计学意义(P＜0.05)。
结论:青春期接受酒精刺激的大鼠,成年后海马结构CA_3、齿状回突触囊泡素表达减少,Morris水迷宫测试中的逃避潜伏期延长,空间学习与记忆能力下降。
Objective:To study the effect of adolescent alcohol stimulation on the spatial learning and memory function and the expression of synaptophysin in the hippocampal formation neurons of adult rats.
Method:Adolescent SD rats were divided into two groups:saline or alcohol(2g/kg),20 rats in each group.Rats of alcohol group were administered ethanol by intraperitional injection,and the process was repeated daily for 5 continuous days,twice everyday,while rats of control group were received isovolumetric saline injection on the same time,then all of rats were raised normally.When they maturated,rats were tested in Morris water maze for 3 days,and recorded a video of every animal behavior,then the result of Morris water maze was analyzed by using software SPSS 11.5.The hippocampal formation of every rat was tested immunohistochemistry of synaptophysin.
Result:With the times of training increased,the escape latency of rats of each group were shortened.Significanted differences of escape latency were found between control and alcohol group(P＜0.05),the escape latency of alcohol group is longer than that of control group.
The expression of the immunohistochemistry of synaptophysin:The positive immunoproduct of synaptophysin mainly express in the CA_3 and gyrus dentatus of the hippocampal formation.They were brown drops and arranged a laminar shape.There is a significant difference between control and alcohol group of synaptophysin content in the hippocampal formation(P＜0.05).
Conclusion:The expression of synaptophysin was decreased in CA_3 and gyrus dentatus of the hippocampal formation of adult rats which had ever received alcohol stimulation in adolescent,the escape latency in Morris Water maze was prolonged,and the capability of the spacial learning and memory was decreased.
方法:取青春期40只SD大鼠分为对照组与实验组,两组动物分别以生理盐水、2g/kg酒精连续注射5天,每天固定时间2次,然后正常饲养,待成年后用Morris水迷宫对各组大鼠进行空间辨别性学习记忆的测试,用摄像机拍摄大鼠的行为,再用统计分析软件分析水迷宫检测结果。用免疫组织化学方法检测正常对照组与实验组大鼠海马结构突触囊泡素的表达。
结果:Morris水迷宫结果:各组大鼠随着训练次数的增加逃避潜伏期(EL)所用时间逐渐缩短。酒精组逃避潜伏期(EL)比正常对照组长,差异有统计学意义(P＜0.05)。
突触囊泡素免疫表达:突触囊泡素的免疫表达主要集中表达在海马结构的CA_3以及齿状回,为褐色颗粒状,呈板状分布。正常对照组与酒精组比较,大鼠海马结构CA_3跟齿状回突触囊泡素免疫反应阳性产物的平均灰度值差异有统计学意义(P＜0.05)。
结论:青春期接受酒精刺激的大鼠,成年后海马结构CA_3、齿状回突触囊泡素表达减少,Morris水迷宫测试中的逃避潜伏期延长,空间学习与记忆能力下降。
Objective:To study the effect of adolescent alcohol stimulation on the spatial learning and memory function and the expression of synaptophysin in the hippocampal formation neurons of adult rats.
Method:Adolescent SD rats were divided into two groups:saline or alcohol(2g/kg),20 rats in each group.Rats of alcohol group were administered ethanol by intraperitional injection,and the process was repeated daily for 5 continuous days,twice everyday,while rats of control group were received isovolumetric saline injection on the same time,then all of rats were raised normally.When they maturated,rats were tested in Morris water maze for 3 days,and recorded a video of every animal behavior,then the result of Morris water maze was analyzed by using software SPSS 11.5.The hippocampal formation of every rat was tested immunohistochemistry of synaptophysin.
Result:With the times of training increased,the escape latency of rats of each group were shortened.Significanted differences of escape latency were found between control and alcohol group(P＜0.05),the escape latency of alcohol group is longer than that of control group.
The expression of the immunohistochemistry of synaptophysin:The positive immunoproduct of synaptophysin mainly express in the CA_3 and gyrus dentatus of the hippocampal formation.They were brown drops and arranged a laminar shape.There is a significant difference between control and alcohol group of synaptophysin content in the hippocampal formation(P＜0.05).
Conclusion:The expression of synaptophysin was decreased in CA_3 and gyrus dentatus of the hippocampal formation of adult rats which had ever received alcohol stimulation in adolescent,the escape latency in Morris Water maze was prolonged,and the capability of the spacial learning and memory was decreased.
引文
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[2]张河川,赵虹.3595名青少年烟酒滥用的流行病学调查.中国校医,1999,13:86-89
[3]连智,刘志民.中学生对药物滥用认知、态度的调查.中国药物滥用防止杂志,2001,30(1):31-34
[4]苏中华,郝伟.酒精性认知损害的研究进展.国外医学精神病学分册,2005,32(1):49-52
[5]Melia KR,Ryabinin AE,Cordimas KP,Wilson MC,Ledoux JE:Hippocampal-dependent learning and experience-dependent activation of the hippocampus are preferentially disrupted by ethanol.Neuroscience1996(74)313-322
[6]Bast T,Zhang WN,Feldon J.Dorsal hippocampus and classical fear conditioning to tone and context in rats:effects of local NMDA-receptor blockade and stimulation.Hippocampus.2003,13(6):657-675
[7]Acheson SK,Ross EL,Swartzwelder HS Age-independent and dose-response effects of ethanol on spatial memory in rats.Alcohol 2001(23):167-175
[8]Wang DG,Song YF,ZhangWS,et al.Effects of paradoxical sleep deprivation on hippocampal synaptophysin level in rats.J Fourth M ilM ed Univ,2004,25(2):111-113
[9]Weitemier AZ,Ryabinin AE.Alcohol-induced memory a impairment in trace fear conditioning:a hippocampus-specific effect.Hippocampus.2003,13(3):305-315
[10]Sarnat HB,Born DE.Synaptophysin immunocytochemistry with thermal intensification:a marker of terminal axonal maturation in the human fetal nervous system.Brain Dec,1999,21:41-50
[11]厉有明 酒精致多脏器损伤实验研究 医学研究通讯,2005,34(10):40-41
[12]李芳芳,张宇,陈韶华,等 酒精灌胃大鼠全血乙醛浓度变化 浙江预防医学,2004,16(1):13-14
[13]赵静波,王泰龄,张晶等 大鼠急性酒精性肝损伤模型分析 中日友好医院学报,1996,10(1):17-19
[14]Little PJ,Kuhn CM.,Wilson WA,Swartzwelder HS.Differential effects of ethanol in adolescent and adult rats.Alcohol ClinExp Res 20:1346-1351
[15]Sircar R,Follesa P,Ticku MK.Chronic neonatal PCP differentially regulates mRNA levels for NMDA receptor subunits Mol Brain Res 40:214-220
[16]Lesspr RA,Mattson MV,Rice KC,Jacobson AE Electrophilic affinity ligands or the phencyclidine(PCP)receptor NIDA Res Monogr 1986,67:138-144
[17]张晨,凌冰 氟一砷联合染毒对大鼠子代神经行为发育的影响 卫生研究,1999,28:337-338
[18]Matthewsa DB,IlgenL M,White AM,Best PJ Acute ethanol administration impairs spatial performance while facilitating non-spatial performance in rats.Neurobiol Learn Mem 1999(72):169-179
[19]宿宝贵,许鹿希 大鼠海马结构在空间辨别性学习记忆时的细胞形态学观察解剖学杂志,2003 26(1):35-39
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