Fenton氧化对微生物群落结构和功能基因的影响
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摘要
通过使用H_2O_2和Na_2CO_3·1.5H_2O_2所形成的两种类Fenton试剂对原油污染的土壤进行氧化修复,在比较两种试剂氧化效果的基础上,采用16s rDNA技术对氧化后土壤中土著微生物的群落结构和功能基因的影响进行了比较。结果表明:Na_2CO_3·1.5H_2O_2所形成的类Fenton试剂对原油的降解效果较好,原油降解率达到50.48%;两种试剂氧化后土壤中的优势菌种均发生变化,由原来的放线菌门(Actinobacteria)变为变形菌门(Proteobacteria),同时氧化后土壤中的总功能基因含量下降,由原来的58 093 193个分别下降到36 282 211个和32 355 524个,而各种通路基因在总基因的占比变化不大。
In this paper,two kinds of Fenton reagents formed by H_2O_2 and Na_2 CO_3·1.5 H_2O_2 were used to oxidize and remediate crude oilcontaminated soils.Based on the comparison of the oxidation effects of the two reagents,16 s rDNA technology was applied to evaluate the community structure and functional genes of indigenous microorganisms in oxidized soils.The results showed that,the Fenton-like reagent formed by Na_2 CO_3·1.5 H_2O_2 had a better degradation effect on crude oil,with a degradation rate of reached 50.48%.The dominant bacteria in soil changed from Actinobacteria to Proteobacteria after oxidation of the two reagents.The content of total functional genes decreased from 58093193 to 3628211 and3235524,respectively,while the proportion of various pathway genes in total genes remained relatively stable.
In this paper,two kinds of Fenton reagents formed by H_2O_2 and Na_2 CO_3·1.5 H_2O_2 were used to oxidize and remediate crude oilcontaminated soils.Based on the comparison of the oxidation effects of the two reagents,16 s rDNA technology was applied to evaluate the community structure and functional genes of indigenous microorganisms in oxidized soils.The results showed that,the Fenton-like reagent formed by Na_2 CO_3·1.5 H_2O_2 had a better degradation effect on crude oil,with a degradation rate of reached 50.48%.The dominant bacteria in soil changed from Actinobacteria to Proteobacteria after oxidation of the two reagents.The content of total functional genes decreased from 58093193 to 3628211 and3235524,respectively,while the proportion of various pathway genes in total genes remained relatively stable.
引文
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[2] 崔丽虹.石油烃降解菌的筛选、鉴定及复合菌群降解效果的研究[D].北京:中国农业科学院,2009:1-56.
[3] 韩寒冰,刘杰凤.机油降解菌的分离及其降解特性研究[J].安徽农业科学,2009,37(21):9883-9884.
[4] LU M,ZHANG Z,QIAO W,et al.Remediation of petroleum-contaminated soil after composting by sequential treatment with Fenton-like oxidation and biodegradation[J].Bioresource technology,2010,101(7):2106-2113.
[5] CHEN M,XU P,ZENG G,et al.Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons,petroleum,pesticides,chlorophenols and heavy metals by composting:Applications,microbes and future research needs[J].Biotechnology advances,2015,33(6):745-755.
[6] TZAITANG T,CHIHMING K,TZUNGYUH Y,et al.Remediation of fuel oil-contaminated soils by a three-stage treatment system[J].Environmental engineering science,2009,26(3):651-659.
[7] 薛蓉蓉.克拉玛依石油污染土壤石油烃降解菌及其降解特性研究[D].青岛:中国石油大学(华东),2010:1.
[8] 孙燕英,陈鸿汉,何炜.土壤中石油类污染物的化学氧化去除研究[J].土壤,2008,40(1):130-134.
[9] KOMMALAPATI R R,VALSARAJ K T,CONSTANT W D,et al.Aqueous solubility enhancement and desorption of hexachlorobenzene from soil using a plant-based surfactant[J].Water research,1997,31(9):2161-2170.
[10] SRA K S,THOMSON N R,BARKER J F.Persulfate injection into a gasoline source zone[J].Journal of contaminant hydrology,2013,150(5):35-44.
[11] WOLICKA D,SUSZEK A,BORKOWSKI A,et al.Application of aerobic microorganisms in bioremediation in situ of soil contaminated by petroleum products.[J].Bioresource technology,2009,100(13):3221-3227.
[12] YEN C H,CHEN K F,KAO C M,et al.Application of persulfate to remediate petroleum hydrocarbon-contaminated soil:Feasibility and comparison with common oxidants[J].Journal of hazardous materials,2011,186:2097-2102.
[13] 金学锋,张国华,乔昌明,等.类芬顿试剂应用于地下水石油烃污染修复的实践[J].中国资源综合利用,2017,35(2):15-17.
[14] 徐金兰,黄廷林,GROTENHUIS T.呼吸试验评价化学氧化前后石油污染土壤微生物活性的研究[J].土木工程学报,2010(增刊):440-444.
[15] 占升,郑义,李森,等.不同氧化剂活化过硫酸钠对土壤中多环芳烃降解的影响[J].浙江农业学报,2017,29(1):129-136.
[16] 陈昌照,于文赫,陈宏坤,等.热洗后油泥砂化学氧化处理可行性研究[J].油气田环境保护,2017,27(2):14-17.
[17] JURADO M,LOPEZ M J,SUAREZESTRELLA F,et al.Exploiting composting biodiversity:Study of the persistent and biotechnologically relevant microorganisms from lignocellulose-based composting[J].Bioresource technology,2014,162(6):283-293.
[18] 王东旭.基于KEGG的代谢通路最短路径问题的研究[D].哈尔滨:哈尔滨工业大学,2007:1-44.
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