摘要
为促进泥鳅养殖业的健康发展,分别挑选体表具有明显出血症状及体表溃烂症状的患病泥鳅对其进行解剖和观察。分离病原菌并进行纯培养,运用多种方法对病原菌进行鉴定,包括革兰氏染色法、三糖铁琼脂生化实验、PCR的扩增和测序等。结果表明:出血症致病菌为维氏气单胞菌,体表溃烂的致病菌为腐败希瓦氏菌。
In order to promote the healthy development of loach breeding industry,we selected the diseased loaches with the body surface has obvious bleeding symptoms or the body surface has the symptoms of ulceration for anatomy and physiological observation.The pathogenic bacteria were isolated and purified,various methods were used to identify the pathogens,including Gram staining,three sugar iron agar biochemical experiment,PCR amplification and sequencing.The results showed that the pathogenic bacteria of bleeding symptoms was Aeromonas veronii,and the pathogenic bacteria of body surface ulceration was Shewanella putrefaciens.
引文
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