紫叶稠李叶片对低温环境的生理响应
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摘要
【目的】对紫叶稠李进行低温处理,检测花色素苷、叶绿素、类胡萝卜素和可溶性糖积累的变化,以及相关酶活性响应规律。【方法】用低温处理紫叶稠李,测定并分析叶色参数、花色素苷、叶绿素、类胡萝卜素、超氧化物歧化酶活性、过氧化物酶活性、苯丙氨酸解氨酶活性、多酚氧化酶活性、可溶性糖含量等生理生化指标的变化规律。【结果】低温环境下紫叶稠李花色素苷、叶绿素、类胡萝卜素、可溶性糖含量增加,超氧化物歧化酶和过氧化物酶活性增加,表明紫叶稠李在低温胁迫下通过提高色素积累和增加可溶性糖含量,提高自身的耐寒性。【结论】紫叶稠李在初夏叶片变色期受低温处理时,低温并没有影响它的叶片变色规律和观赏性,本研究为今后提高紫叶稠李的引种适用性和观赏性应用提供理论依据。
【Objective】Prunus virginianais treated in low temperature,study on the changes of temperature on the accumulation of anthocyanins,chlorophyll,carotenoids and soluble sugars,and the changes of related enzyme activities.【Methods】Prunus virginiana thick plum is treated at low temperature.The changes of leaf color parameters,anthocyanins,chlorophyll,carotenoids,SOD,POD,PAL,PPO,soluble sugar content and other physiological and biochemical indexes were determined and analyzed.【Results】Under low temperature,the content of anthocyanins,chlorophyll,carotenoids and soluble sugars increased,and the activities of SOD and POD increased.Under low temperature stress,Prunus virginiana can improve its cold tolerance by increasing pigment accumulation and increasing soluble sugar content.【Conclusion】Prunus virginianathick plum is treated with low temperature during the discoloration period of early summer leaves.Low temperature does not affect its leaf discoloration and ornamental,This study provides a theoretical basis for improving the applicability and ornamental application of Prunus virginiana.
【Objective】Prunus virginianais treated in low temperature,study on the changes of temperature on the accumulation of anthocyanins,chlorophyll,carotenoids and soluble sugars,and the changes of related enzyme activities.【Methods】Prunus virginiana thick plum is treated at low temperature.The changes of leaf color parameters,anthocyanins,chlorophyll,carotenoids,SOD,POD,PAL,PPO,soluble sugar content and other physiological and biochemical indexes were determined and analyzed.【Results】Under low temperature,the content of anthocyanins,chlorophyll,carotenoids and soluble sugars increased,and the activities of SOD and POD increased.Under low temperature stress,Prunus virginiana can improve its cold tolerance by increasing pigment accumulation and increasing soluble sugar content.【Conclusion】Prunus virginianathick plum is treated with low temperature during the discoloration period of early summer leaves.Low temperature does not affect its leaf discoloration and ornamental,This study provides a theoretical basis for improving the applicability and ornamental application of Prunus virginiana.
引文
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[2]刘广林,梅梅,罗东明,陆秀君,曹婷.紫叶稠李在沈阳地区越冬安全性分析[J].沈阳农业大学学报,2014(6):696-700
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[11]付全娟,魏国芹,孙杨,张立华,孙玉刚.花青苷在果树抗逆中的作用研究进展[J].落叶果树,2015(6):21-24
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[13]曾乃燕,何军贤,赵文,梁厚果.低温胁迫期间水稻光合膜色素与蛋白水平的变化[J].西北植物学报,2000,20(1):8-14
[14]石进朝,李桂伶,陈秀新,李彦侠.长绿期金银木耐寒生理机制研究[J].西北植物学报,2009,29(1):111-115
[15]姚小红.环境因子对彩叶扶桑(Hibiscus rosa-sinensis var.variegate)叶色生理变化影响的研究[D].福州:福建农林大学,2014
[16]欧文军,罗秀芹,李开绵.低温胁迫对木薯组培苗叶片若干代谢生理指标的影响[J].热带作物学报,2015,36(4):706-712
[17]李春燕,徐雯,刘立伟,杨景,朱新开,郭文善.低温条件下拔节期小麦叶片内源激素含量和抗氧化酶活性的变化[J].应用生态学报,2015,26(7):2015-2022
[18]陈荣发,刘文娟,张保青,杨丽涛,李杨瑞.低温胁迫对甘蔗幼苗根系线粒体生理生化作用的影响[J].南方农业学报,2015,46(8):1385-1390
[19]Babitha M P,Bhat S G,Prakash H S,Shetty H S.Differential induction of superoxide dismutase in downy mildew-resistant and susceptible genotypes of pearl millet[J].Plant Pathology,2002,51(4):480-486
[20]Kukavica B,Jovanovic S V.Senescence-related changes in the antioxidant status of ginkgo and birch leaves during autumn yellowing[J].Physiologia Plantarum,2004,122(3):321-327
[21]冯定邦,黄继川,彭智平,徐培智,张仁陟.淹涝胁迫后施用不同有机碳(氮)营养对水稻主要抗逆生理指标的影响[J].南方农业学报,2015,46(12):2106-2111
[22]巫光宏,罗焕亮.几种保护酶活性变化与马占相思树对低温胁迫的抵抗性的关系研究[J].植物研究,2002,22(1):42-45
[23]朱宁华,李志辉,李芳东.桉树耐寒性与超氧化物歧化酶关系研究[J].中南林业科技大学学报,2000,20(3):63-66
[24]刘鹏,曹林,王利华,刘丹,徐根娣,马丽,郦枫.低温胁迫下外源水杨酸对栝楼幼苗抗寒性影响[J].浙江师范大学学报(自然科学版),2015(1):9-14
[2]刘广林,梅梅,罗东明,陆秀君,曹婷.紫叶稠李在沈阳地区越冬安全性分析[J].沈阳农业大学学报,2014(6):696-700
[3]梁冰,李湛东,陈建芳,赵蓓蓓,沙海峰.北京地区彩叶树种应用的综合评价[J].广东农业学,2014(4):64-67,92
[4]刘智勇.浅谈北京大规格彩叶树种的应用及前景[J].绿化与生活,2016(5):15-16
[5]王晶英,敖红,张杰.植物生理生化实验技术与原理[M].哈尔滨:东北林业大学出版社,2003
[6]王秀兰.紫叶稠李在沈阳的引种及繁殖技术研究[J].林业勘查设计,2009(1):112-113
[7]李合生.植物生理生化试验原理与技术[M].北京:高等教育出版社,2000:165-169
[8]刘家尧.植物生理实验教程[M].北京:高等教育出版社,2010:79-80
[9]高佩刚.6种地被观赏竹对低温胁迫的生理响应研究[D].雅安:四川农业大学,2014
[10]杨希,隆学武,曾华浩,黄勇,范宏达.美丽针葵耐寒性试验[J].福建林业科技,2002,29(2):56-57
[11]付全娟,魏国芹,孙杨,张立华,孙玉刚.花青苷在果树抗逆中的作用研究进展[J].落叶果树,2015(6):21-24
[12]刘国君,仵春涛,徐斌.小苹果休眠期枝条花青素和叶绿素的含量变化与抗寒关系[J].北方园艺,2002(5):58-59
[13]曾乃燕,何军贤,赵文,梁厚果.低温胁迫期间水稻光合膜色素与蛋白水平的变化[J].西北植物学报,2000,20(1):8-14
[14]石进朝,李桂伶,陈秀新,李彦侠.长绿期金银木耐寒生理机制研究[J].西北植物学报,2009,29(1):111-115
[15]姚小红.环境因子对彩叶扶桑(Hibiscus rosa-sinensis var.variegate)叶色生理变化影响的研究[D].福州:福建农林大学,2014
[16]欧文军,罗秀芹,李开绵.低温胁迫对木薯组培苗叶片若干代谢生理指标的影响[J].热带作物学报,2015,36(4):706-712
[17]李春燕,徐雯,刘立伟,杨景,朱新开,郭文善.低温条件下拔节期小麦叶片内源激素含量和抗氧化酶活性的变化[J].应用生态学报,2015,26(7):2015-2022
[18]陈荣发,刘文娟,张保青,杨丽涛,李杨瑞.低温胁迫对甘蔗幼苗根系线粒体生理生化作用的影响[J].南方农业学报,2015,46(8):1385-1390
[19]Babitha M P,Bhat S G,Prakash H S,Shetty H S.Differential induction of superoxide dismutase in downy mildew-resistant and susceptible genotypes of pearl millet[J].Plant Pathology,2002,51(4):480-486
[20]Kukavica B,Jovanovic S V.Senescence-related changes in the antioxidant status of ginkgo and birch leaves during autumn yellowing[J].Physiologia Plantarum,2004,122(3):321-327
[21]冯定邦,黄继川,彭智平,徐培智,张仁陟.淹涝胁迫后施用不同有机碳(氮)营养对水稻主要抗逆生理指标的影响[J].南方农业学报,2015,46(12):2106-2111
[22]巫光宏,罗焕亮.几种保护酶活性变化与马占相思树对低温胁迫的抵抗性的关系研究[J].植物研究,2002,22(1):42-45
[23]朱宁华,李志辉,李芳东.桉树耐寒性与超氧化物歧化酶关系研究[J].中南林业科技大学学报,2000,20(3):63-66
[24]刘鹏,曹林,王利华,刘丹,徐根娣,马丽,郦枫.低温胁迫下外源水杨酸对栝楼幼苗抗寒性影响[J].浙江师范大学学报(自然科学版),2015(1):9-14
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