不同施肥处理方式对草莓根际土壤酶活性的影响
|
摘要
目的:旨在探讨不同施肥处理方式对草莓根际土壤酶活性的影响。方法:以"红颜"草莓为材料,采用盆栽试验,设计化肥(CK)、有机肥(T1)、有机肥+生物炭(T2)、无肥(T3) 4个处理,探讨不同施肥处理方式对草莓根际土壤β-葡萄糖苷酶、N-乙酰-β-葡萄糖苷酶、酚氧化酶、过氧化物酶、脲酶和蛋白酶活性的影响。结果:1)与CK对比,T1处理的草莓根际土壤β-葡萄糖苷酶、N-乙酰-β-葡萄糖苷酶、酚氧化酶及蛋白酶活性分别降低了30. 77%、8. 33%、48. 24%和3. 54%,而过氧化物酶及脲酶活性分别提高了250%和225%,说明有机肥改土具有显著提升草莓根际土壤过氧化物酶及脲酶活性的作用; 2)与CK对比,T2处理的草莓根际土壤β-葡萄糖苷酶、N-乙酰-β-葡萄糖苷酶活性分别降低了43. 59%和9. 52%,而酚氧化酶、过氧化物酶、脲酶和蛋白酶活性分别提高了295%、543%、175%和8. 85%,说明有机肥与生物炭配施能显著提高草莓根际土壤酚氧化酶(P <0. 05)和过氧化物酶(P <0. 05)的活性,有利于草莓根际土壤脲酶和蛋白酶活性的提高。结论:有机肥配施生物炭的施肥处理方式具有提高草莓根际土壤酚氧化酶、过氧化物酶、脲酶和蛋白酶活性的作用。
Objectives: The aim of this study is to investigate the effect of different fertilization treatments on soil enzyme activities in strawberry rhizosphere. Methods: Using " Hongyan" strawberry as material,pot experiment,arrange chemical fertilizer( CK),organic fertilizer( T1),organic fertilizer + biochar( T2),no fertilizer( T3)treatment,and explore different treatments on strawberry rhizosphere soil β-glucosidase,N-acetyl-β-glucosidase,phenol oxidase,peroxidase,urease and protease activity. Results: 1) Compared with CK,T1 treated soil β-glucosidase,N-acetyl-β-glucosidase,phenol oxidase and protease activities decreased by 30. 77%,8. 33%,48. 24% and 3. 54%,respectively. The activity of peroxide and urease increased by 250% and 225%,respectively. Organic fertilizer changed soil significantly increased the activity of peroxidase and urease in strawberry rhizosphere soil; 2) T2 treated soil β-glucosidase compared with CK N-acetyl-β-glucosidase activity decreased by 43. 59% and 9. 52%,respectively,but phenol oxidase,peroxidase,urease and protease activities increased by 295%,543%,175% and 8. 85%,respectively,indicating the organic soil-modified treatment with fertilizer and biochar can significantly increase the activity of phenoloxidase( P < 0. 05) and peroxidase( P <0. 05),which is beneficial to the improvement of soil urease and protease activity. Conclutions: Soil-removing measures of organic manure combined with biochar have the effects of increasing phenoloxidase,peroxidase,urease and protease enzyme activities in strawberry rhizosphere soil.
Objectives: The aim of this study is to investigate the effect of different fertilization treatments on soil enzyme activities in strawberry rhizosphere. Methods: Using " Hongyan" strawberry as material,pot experiment,arrange chemical fertilizer( CK),organic fertilizer( T1),organic fertilizer + biochar( T2),no fertilizer( T3)treatment,and explore different treatments on strawberry rhizosphere soil β-glucosidase,N-acetyl-β-glucosidase,phenol oxidase,peroxidase,urease and protease activity. Results: 1) Compared with CK,T1 treated soil β-glucosidase,N-acetyl-β-glucosidase,phenol oxidase and protease activities decreased by 30. 77%,8. 33%,48. 24% and 3. 54%,respectively. The activity of peroxide and urease increased by 250% and 225%,respectively. Organic fertilizer changed soil significantly increased the activity of peroxidase and urease in strawberry rhizosphere soil; 2) T2 treated soil β-glucosidase compared with CK N-acetyl-β-glucosidase activity decreased by 43. 59% and 9. 52%,respectively,but phenol oxidase,peroxidase,urease and protease activities increased by 295%,543%,175% and 8. 85%,respectively,indicating the organic soil-modified treatment with fertilizer and biochar can significantly increase the activity of phenoloxidase( P < 0. 05) and peroxidase( P <0. 05),which is beneficial to the improvement of soil urease and protease activity. Conclutions: Soil-removing measures of organic manure combined with biochar have the effects of increasing phenoloxidase,peroxidase,urease and protease enzyme activities in strawberry rhizosphere soil.
引文
[1]葛继红,周曙东.要素市场扭曲是否激发了农业面源污染——以化肥为例[J].农业经济问题,2012,33(3):92-98,112.
[2]沈华,龚佩华,曹国华.大棚草莓生产中存在的问题与栽培技术[J].现代农业科技,2015,(21):73-74.
[3]江琳琳.生物炭对土壤微生物多样性和群落结构的影响[D].沈阳农业大学,2016.
[4]卜晓莉,薛建辉.生物炭对土壤生境及植物生长影响的研究进展[J].生态环境学报,2014,23(3):535-540.
[5]胡诚,刘东海,陈云峰,等.不同土壤改良措施对低产黄泥田土壤性质及水稻产量的影响[J].中国土壤与肥料,2016,(3):117-121.
[6]李凤霞,王学琴,郭永忠,等.不同改良措施对银川平原盐碱地土壤性质及酶活性的影响[J].水土保持研究,2012,19(6):13-18.
[7]安东.不同土壤改良剂对银川平原北部盐碱土水分性质和土壤结构的影响[D].西北农林科技大学,2009.
[8]张宝娟,郭耸松,李继泉,等.掺土和施肥对铁尾矿基质理化性质及油松、樟子松幼苗生长的影响[J].林业科学,2015,51(5):12-20.
[9] Piktel E,Niemirowicz K,Wnorowska U,et al. The Role of Cathelicidin LL-37 in Cancer Development[J]. Archivum Immunologiae Et Therapiae Experimentalis,2016,64(1):33-46.
[10]田小明,李俊华,王成,等.连续3年施用生物有机肥对土壤养分、微生物生物量及酶活性的影响[J].园艺学报,2014,46(3):481-488.
[11]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.
[12]王满,卫宣志,郭芳阳,等.动植物有机肥对烤烟根际土壤酶活性及微生物碳氮的影响[J].江西农业学报,2017,29(10):63-66.
[13]袁中友,梁中龙,杨淇钧,等.有机肥对高速公路建设损毁土壤的微生物学性状及酶活性的影响[J].华南农业大学学报,2017,38(6):58-63.
[14]阮爱东,包天力,刘忱潇,等.烟碱污染对土壤细菌功能酶活性的影响[J].四川环境,2015,34(5):7-11.
[15]田给林,严婷婷,毕艳孟,等.草莓连作土壤灭菌与施用有机肥对根际土壤酚酸及土壤酶活性的影响[J].园艺学报,2015,42(10):2039-2048.
[16]柳燕兰.长期定位施肥土壤酶活性及其肥力变化研究[D].西北农林科技大学,2009.
[17]龚丝雨,聂亚平,张启明,等.增施生物炭对烤烟成熟期根际土壤酶活性的影响[J].江西农业学报,2017,29(10):54-57.
[2]沈华,龚佩华,曹国华.大棚草莓生产中存在的问题与栽培技术[J].现代农业科技,2015,(21):73-74.
[3]江琳琳.生物炭对土壤微生物多样性和群落结构的影响[D].沈阳农业大学,2016.
[4]卜晓莉,薛建辉.生物炭对土壤生境及植物生长影响的研究进展[J].生态环境学报,2014,23(3):535-540.
[5]胡诚,刘东海,陈云峰,等.不同土壤改良措施对低产黄泥田土壤性质及水稻产量的影响[J].中国土壤与肥料,2016,(3):117-121.
[6]李凤霞,王学琴,郭永忠,等.不同改良措施对银川平原盐碱地土壤性质及酶活性的影响[J].水土保持研究,2012,19(6):13-18.
[7]安东.不同土壤改良剂对银川平原北部盐碱土水分性质和土壤结构的影响[D].西北农林科技大学,2009.
[8]张宝娟,郭耸松,李继泉,等.掺土和施肥对铁尾矿基质理化性质及油松、樟子松幼苗生长的影响[J].林业科学,2015,51(5):12-20.
[9] Piktel E,Niemirowicz K,Wnorowska U,et al. The Role of Cathelicidin LL-37 in Cancer Development[J]. Archivum Immunologiae Et Therapiae Experimentalis,2016,64(1):33-46.
[10]田小明,李俊华,王成,等.连续3年施用生物有机肥对土壤养分、微生物生物量及酶活性的影响[J].园艺学报,2014,46(3):481-488.
[11]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.
[12]王满,卫宣志,郭芳阳,等.动植物有机肥对烤烟根际土壤酶活性及微生物碳氮的影响[J].江西农业学报,2017,29(10):63-66.
[13]袁中友,梁中龙,杨淇钧,等.有机肥对高速公路建设损毁土壤的微生物学性状及酶活性的影响[J].华南农业大学学报,2017,38(6):58-63.
[14]阮爱东,包天力,刘忱潇,等.烟碱污染对土壤细菌功能酶活性的影响[J].四川环境,2015,34(5):7-11.
[15]田给林,严婷婷,毕艳孟,等.草莓连作土壤灭菌与施用有机肥对根际土壤酚酸及土壤酶活性的影响[J].园艺学报,2015,42(10):2039-2048.
[16]柳燕兰.长期定位施肥土壤酶活性及其肥力变化研究[D].西北农林科技大学,2009.
[17]龚丝雨,聂亚平,张启明,等.增施生物炭对烤烟成熟期根际土壤酶活性的影响[J].江西农业学报,2017,29(10):54-57.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |