根际解钾菌对烟草生长及钾素吸收的影响
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摘要
选取前期从烟草根际筛选的4株烟草特异性解钾菌TK5、TK37、TK57和TK89菌株,考察这些菌株对烟草生长及钾素吸收的影响。结果表明,4株解钾菌对烟草生长及钾素吸收均有一定的促进作用,其中TK89菌株效果最佳,接种TK89的烟草植株其上部叶鲜重、中部叶鲜重、下部叶鲜重、根系鲜重、上部叶干重、中部叶干重、下部叶干重、根系干重、叶片含钾量及土壤速效钾含量分别较未施菌的烟草植株提高64.99%、27.16%、54.22%、54.70%、67.78%、27.56%、54.20%、50.81%、33.60%和15.79%,各项指标较对照差异均达显著水平,4株解钾菌对烟草根系生长及叶片含钾量均有显著促进作用。解钾菌可促进烟草对土壤钾素营养的吸收,促进烟草生长,提高烟叶含钾量。
Four tobacco specific potassium-releasing bacteria strains TK5, TK37, TK57 and TK89, which were selected from tobacco rhizosphere in our laboratory before, were applied to investigate the effects of these strains on the growth and potassium absorption of tobacco plants. As results, all the four potassium-releasing bacteria had a certain promotion function to the growth and potassium absorption of tobacco plants, and among which, strain TK89 had the best promoting effects. The fresh weight of upper leaves, middle leaves, lower leaves and roots of tobacco plants, the dry weight of upper leaves, middle leaves, lower leaves and roots of tobacco plants, the potassium content in tobacco leaves and the available potassium content in soil were 64.99%, 27.16%, 54.22%, 54.70%, 67.78%, 27.56%, 54.20%, 50.81%, 33.60% and 15.79% greater, respectively, for the tobacco plants inoculated with TK89 than in the control plants. The development of tobacco roots and potassium contents in leaves was significantly promoted by all the four potassium-releasing bacteria. The pot experiment showed that potassium-releasing bacteria tested in this study can promote the absorption of potassium in soil, the growth of tobacco and the increase of potassium content in tobacco leaves.
Four tobacco specific potassium-releasing bacteria strains TK5, TK37, TK57 and TK89, which were selected from tobacco rhizosphere in our laboratory before, were applied to investigate the effects of these strains on the growth and potassium absorption of tobacco plants. As results, all the four potassium-releasing bacteria had a certain promotion function to the growth and potassium absorption of tobacco plants, and among which, strain TK89 had the best promoting effects. The fresh weight of upper leaves, middle leaves, lower leaves and roots of tobacco plants, the dry weight of upper leaves, middle leaves, lower leaves and roots of tobacco plants, the potassium content in tobacco leaves and the available potassium content in soil were 64.99%, 27.16%, 54.22%, 54.70%, 67.78%, 27.56%, 54.20%, 50.81%, 33.60% and 15.79% greater, respectively, for the tobacco plants inoculated with TK89 than in the control plants. The development of tobacco roots and potassium contents in leaves was significantly promoted by all the four potassium-releasing bacteria. The pot experiment showed that potassium-releasing bacteria tested in this study can promote the absorption of potassium in soil, the growth of tobacco and the increase of potassium content in tobacco leaves.
引文
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[21]崔松松,白莉敏,周可金,等.油菜亲和性根际菌株的筛选及其促生效果[J].西北农林科技大学学报(自然科学版),2016,44(1):91-96.
[22]那文志,徐凤花,鲍玉杰,等.硅酸盐菌剂的解钾作用及田间施用效果研究[J].生命科学仪器,2009,7(4):38-41.
[2]周克清.烟草行业对国家财政的贡献度研究[J].光华财税年刊,2010:268-274.
[3]刘正日,胡日生,郭清泉.烟草钾营养研究进展[J].作物研究,2004,18(2):109-114.
[4]欧清华.根外追施钾肥对烤烟产质量的影响研究进展[J].天津农业科学,2013,19(7):30-34.
[5]叶协锋,朱海滨,凌爱芬,等.不同钾肥对烤烟叶片钾和中性香气成分及非挥发性有机酸含量的影响研究[J].土壤通报,2008,39(2):338-343.
[6]李强,周冀衡,何伟,等.中国烤烟含钾量的区域特征研究[J].安徽农业大学学报,2010,37(2):363-368.
[7]磷钾研究所.土壤肥力手册[M].加拿大钾磷肥研究所北京办事处编译.1992.
[8]谢如林,谭宏伟,周柳强,等.无径流和淋溶条件下钾肥利用率的研究[J].华南农业大学学报,2005,26(1):36-41.
[9]BAL H B,NAYAK L,DAS S,et al.Isolation of ACCdeaminase producing PGPR from rice rhizosphere and evaluating their plant growth promoting activity under salt stress[J].Plant Soil,2013,366(1/2):93-105.
[10]BHATTACHARYYA P N,JHA D K.Plant growth-promoting rhizobacteria(PGPR):emergence in agriculture[J].World J Microbiol Biotechnol,2012,28(4):1327-1350.
[11]ARIKAN S,PIRLAK L.Effects of plant growth promoting rhizobacteria(PGPR)on growth,yield and fruit quality of Sour Cherry(Prunus cerasus L.)[J].Erwerbs-Obstbau,2016,58(4):221-226.
[12]ZHANG C S,KONG F Y.Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants[J].Appl Soil Ecol,2014,82(7):18-25.
[13]龚文秀,曹媛媛,倪海婷,等.烟草亲和性解钾PGPR菌株筛选及其促生效果研究[J].中国烟草学报,2016,22(1):55-63.
[14]TANG Z H,ZHANG A J,WEI M,et al.Physiological response to potassium deficiency in three sweet potato(Ipomoea batatas[L.]Lam.)genotypes differing in potassium utilization efficiency[J].Acta Physiol Plant,2015,37(8):1-10.
[15]张乃凤.土壤速效钾的测定:醋酸铵浸提、火焰光度计测定法[J].土壤肥料,1974(1):33-35.
[16]DING M M,WEI B,ZHANG Z,et al.Effect of potassium organic and inorganic salts on thermal decomposition of reconstituted tobacco sheet[J].J Therm Anal Calorim,2017,129(2):975-984.
[17]党雯,郜春花,张强,等.解钾菌的研究进展及其在农业生产中的应用[J].山西农业科学,2014,42(8):921-924.
[18]钟冬梅,马光庭.硅酸盐细菌在农业中应用的研究动态[J].广西农学报,2006,23(3):32-35.
[19]陈易,程永毅,郭涛,等.一株具紫色土亲和性解钾菌的筛选及促生效应[J].西南大学学报(自然科学版),2016,(38)5:58-65.
[20]张红娟,张朝阳,聂刚.钾细菌对土壤养分活化作用的研究[J].杨凌职业技术学院学报,2005,4(3):4-6.
[21]崔松松,白莉敏,周可金,等.油菜亲和性根际菌株的筛选及其促生效果[J].西北农林科技大学学报(自然科学版),2016,44(1):91-96.
[22]那文志,徐凤花,鲍玉杰,等.硅酸盐菌剂的解钾作用及田间施用效果研究[J].生命科学仪器,2009,7(4):38-41.
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