基于液相~(31)P核磁共振技术的不同地理区域湖泊沉积物生物质磷(Biogenic-P)形态特征研究
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摘要
利用液相~(31)P核磁共振分析方法,研究了我国不同地理区域湖泊表层沉积物中生物质磷(Biogenic-P)的形态特征.结果表明,不同地理区域湖泊表层沉积物磷含量差异较大,湖泊沉积物中的总磷在184.37～1089.41 mg·kg~(-1)之间,青藏高原的青海湖总磷含量最高,达到1089.41 mg·kg~(-1).使用~(31)P-NMR技术在湖泊沉积物中共检测到4类磷化合物,其中生物质磷检测到3类磷化合物,分别为:磷酸单酯(Mono-P)、DNA磷(DNA-P)、焦磷酸盐(Pyro-P),其中以磷酸单酯为主,含量在5.57～399.18 mg·kg~(-1)之间.随着我国外源性磷的有效控制,内源性磷成为了磷主要的来源,在磷的内源负荷中,生物质磷矿化释放的磷将会影响水体整体生产力,本研究基于液相~(31)P核磁共振技术,对我国不同区域湖泊沉积物中磷形态的分布有了一些新的认识,可为深入认识磷在不同区域湖泊中转化提供支持,有助于深入研究不同区域湖泊磷生物地球化学循环过程,同时为认识我国不同区域湖泊沉积物中磷形态特征提供数据支持.
The morphological characteristics of biogenic phosphorus(Biogenic-P) in surface sediments of lakes in different geographical regions of China were studied by liquid ~(31)P-NMR. The results show that the contents of phosphorus in lake sediments varied greatly in different geographical regions. The total phosphorus in lake sediments ranged from 184.37～1089.41 mg·kg~(-1).The total phosphorus content of Qinghai Lake Tibetan Plateau is the highest, reaching 1089.41 mg·kg~(-1). Four kinds of phosphorus compounds were detected in lake sediments by ~(31)P-NMR. Among them, three kinds of phosphorus compounds were detected in biomass phosphorus, namely, Mono-P, DNA-P and Pyro-P. The content of phosphorus monoester was mainly 5.57～399.18 mg·kg~(-1).With the effective control of exogenous phosphorus, endogenous phosphorus has become the main source of phosphorus in the water column. In the endogenous load of phosphorus, the phosphorus released by the mineralization of biomass phosphorus will affect the overall productivity of water. Based on Liquid ~(31)P NMR technology, we have some new understandings of the distribution of phosphorus forms in lake sediments in different regions of China, which can provide insights in understanding the transformation of phosphorus in lakes, and help to further study the phosphorus bio-earth of lakes. Meanwhile, it helps to understand the phosphorus biogeochemical cycle in lakes and to provide data support for understanding the phosphorus morphological characteristics in lake sediments.
The morphological characteristics of biogenic phosphorus(Biogenic-P) in surface sediments of lakes in different geographical regions of China were studied by liquid ~(31)P-NMR. The results show that the contents of phosphorus in lake sediments varied greatly in different geographical regions. The total phosphorus in lake sediments ranged from 184.37～1089.41 mg·kg~(-1).The total phosphorus content of Qinghai Lake Tibetan Plateau is the highest, reaching 1089.41 mg·kg~(-1). Four kinds of phosphorus compounds were detected in lake sediments by ~(31)P-NMR. Among them, three kinds of phosphorus compounds were detected in biomass phosphorus, namely, Mono-P, DNA-P and Pyro-P. The content of phosphorus monoester was mainly 5.57～399.18 mg·kg~(-1).With the effective control of exogenous phosphorus, endogenous phosphorus has become the main source of phosphorus in the water column. In the endogenous load of phosphorus, the phosphorus released by the mineralization of biomass phosphorus will affect the overall productivity of water. Based on Liquid ~(31)P NMR technology, we have some new understandings of the distribution of phosphorus forms in lake sediments in different regions of China, which can provide insights in understanding the transformation of phosphorus in lakes, and help to further study the phosphorus bio-earth of lakes. Meanwhile, it helps to understand the phosphorus biogeochemical cycle in lakes and to provide data support for understanding the phosphorus morphological characteristics in lake sediments.
引文
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Aspila K I,Agemian H,Chau A S Y.1976.A semi-automated method for the determination of inorganic,organic and total phosphate in sediments[J].Analyst,101(1200):187-197
Bowman R A.1989.A sequential extraction procedure with concentrated sulfuric acid and dilute base for soil organic phosphorus[J].Soil Science Society of America Journal,53(2):362-366
白秀玲,周云凯,李斌,等.2011.基于液相31P核磁共振分析的沉积物样品制备方法研究[J].环境科学,32(7):1980-1985
Bai X,Ding S,Fan C,et al.2009.Organic phosphorus species in surface sediments of a large,shallow,eutrophic lake,Lake Taihu,China[J].Environmental Pollution,157(8/9):2507-2513
Cade-Menun B,Liu C W.2014.Solution phosphorus-31 nuclear magnetic resonance spectroscopy of soils from 2005 to 2013:A review of sample preparation and experimental parameters[J].Soil Science Society of America Journal,78(1):19-37
Cade-Menun B J,Navaratnam J A,Walbridge M R.2006.Characterizing dissolved and particulate phosphorus in water with 31P nuclear magnetic resonance spectroscopy[J].Environmental Science & Technology,40(24):7874-7880
Cardoza L A,Korir A K,Otto W H,et al.2004.Applications of NMR spectroscopy in environmental science[J].Progress in Nuclear Magnetic Resonance Spectroscopy,45(3/4):209-238
Cade-Menun B J,Benitez-Nelson C R,Pellechia P,et al.2005.Refining 31P nuclear magnetic resonance spectroscopy for marine particulate samples:Storage conditions and extraction recovery[J].Marine Chemistry,97(3/4):293-306
Cade-Menun B J.2005.Characterizing phosphorus in environmental and agricultural samples by 31P nuclear magnetic resonance spectroscopy[J].Talanta,66(2):359-371
Ding S,Xu D,Bai X,et al.2013.Speciation of organic phosphorus in a sediment profile of Lake Taihu II.Molecular species and their depth attenuation[J].Journal of Environmental Sciences,25(5):925-932
Dittrich M,Chesnyuk A,Gudimov A,et al.2013.Phosphorus retention in a mesotrophic lake under transient loading conditions:insights from a sediment phosphorus binding form study[J].Water Research,47(3):1433-1447
Dell′Anno A,Danovaro R.2005.Extracellular DNA plays a key role in deep-sea ecosystem functioning[J].Science,309(5744):2179-2179
Huo S,Zan F,Xi B,et al.2011.Phosphorus fractionation in different trophic sediments of lakes from different regions,China[J].Journal of Environmental Monitoring,13(4):1088-1095
Huang Q,Wang Z,Wang C,et al.2005.Phosphorus release in response to pH variation in the lake sedimentswith different ratios of iron-bound P to calcium-bound P[J].Chemical Speciation & Bioavailability,17(2):55-61
Ishii Y,Harigae S,Tanimoto S,et al.2010.Spatial variation of phosphorus fractions in bottom sediments and the potential contributions to eutrophication in shallow lakes[J].Limnology,11(1):5-16
Jensen H S,Kristensen P,Jeppesen E,et al.1992.Iron:phosphorus ratio in surface sediment as an indicator of phosphate release from aerobic sediments in shallow lakes[M]//Sediment/Water Interactions.Springer,Dordrecht,731-743
Kovar J L,Pierzynski G M.2009.Methods of phosphorus analysis for soils,sediments,residuals,and waters second edition[J].Southern cooperative series bulletin,408
金相灿,王圣瑞,庞燕.2004.太湖沉积物磷形态及pH值对磷释放的影响[J].中国环境科学,24(6):707-711
K?iv T,N?ges T,Laas A.2011.Phosphorus retention as a function of external loading,hydraulic turnover time,area and relative depth in 54 lakes and reservoirs[J].Hydrobiologia,660(1):105-115
Kleeberg A,Herzog C,Hupfer M.2013.Redox sensitivity of iron in phosphorus binding does not impede lake restoration[J].Water Research,47(3):1491-1502
李军,刘丛强,王仕禄,等.2004.太湖五里湖表层沉积物中不同形态磷的分布特征[J].矿物学报,24(4):405-410
Liu J,Wang H,Yang H,et al.2009.Detection of phosphorus species in sediments of artificial landscape lakes in China by fractionation and phosphorus-31 nuclear magnetic resonance spectroscopy[J].Environmental Pollution,157(1):49-56
Meyers P A.1994.Preservation of elemental and isotopic source identification of sedimentary organic matter[J].Chemical Geology,114(3/4):289-302
Nyenje P M,Foppen J W,Uhlenbrook S,et al.2010.Eutrophication and nutrient release in urban areas of sub-Saharan Africa—A review[J].Science of the Total Environment,408(3):447-455
Paytan A,McLaughlin K.2007.The oceanic phosphorus cycle[J].Chemical Reviews,107(2):563-576
Paraskova J V,Sj?berg P J R,Rydin E.2014.Turnover of DNA-P and phospholipid-P in lake sediments[J].Biogeochemistry,119(1/3):361-370
钱轶超,陈英旭,楼莉萍,等.2010.核磁共振技术在沉积物磷素组分及迁移转化规律研究中的应用[J].应用生态学报,21(7):1892-1898
Reitzel K,Ahlgren J,DeBrabandere H,et al.2007.Degradation rates of organic phosphorus in lake sediment[J].Biogeochemistry,82(1):15-28
Ruttenberg K C,Goni M A.1997.Phosphorus distribution,C∶N∶P ratios,and δ13Coc in arctic,temperate,and tropical coastal sediments:tools for characterizing bulk sedimentary organic matter[J].Marine Geology,139(1/4):123-145
Shinohara R,Imai A,Kawasaki N,et al.2012.Biogenic phosphorus compounds in sediment and suspended particles in a shallow eutrophic lake:a 31P-nuclear magnetic resonance (31P NMR) study[J].Environmental Science & Technology,46(19):10572-10578
Simpson A J,Simpson M J,Soong R.2012.Nuclear magnetic resonance spectroscopy and its key role in environmental research[J].Environmental Science & Technology,46(21):11488-11496
Salas A M,Elliott E T,Westfall D G,et al.2003.The role of particulate organic matter in phosphorus cycling[J].Soil Science Society of America Journal,67(1):181-189
Smolen J M,Stone A T.1997.Divalent metal ion-catalyzed hydrolysis of phosphorothionate ester pesticides and their corresponding oxonates[J].Environmental Science & Technology,31(6):1664-1673
Turner B L,Mahieu N,Condron L M.2003.Phosphorus-31 nuclear magnetic resonance spectral assignments of phosphorus compounds in soil NaOH-EDTA extracts[J].Soil Science Society of America Journal,67(2):497-510
Turner B L,Cade-Menun B J,Condron L M,et al.2005.Extraction of soil organic phosphorus[J].Talanta,66(2):294-306
Wang Z,Liang Y,Kang W.2011.Utilization of dissolved organic phosphorus by different groups of phytoplankton taxa[J].Harmful Algae,12:113-118
Watts E E,Dean P A W,Martin R R.2002.31P nuclear magnetic resonance study of sediment microbial phospholipids[J].Canadian Journal of Analytical Sciences and Spectroscopy,47(5):127-133
Worsfold P J,Gimbert L J,Mankasingh U,et al.2005.Sampling,sample treatment and quality assurance issues for the determination of phosphorus species in natural waters and soils[J].Talanta,66(2):273-293
杨修,孙芳,任娜.2003.环青海湖地区生态环境问题及其治理对策[J].地域研究与开发,22(2):39-42
闫露霞,孙美平,姚晓军,等.2018.青藏高原湖泊水质变化及现状评价[J].环境科学学报,38(3):900-910
张文强,单保庆.2013.基于液相 31P 核磁共振(NMR)技术的巢湖沉积物中有机磷形态研究[J].环境科学学报,33(7):1967-1973
赵海超,王圣瑞,张莉,等.2014.有机质含量及其组分对洱海沉积物磷吸附-释放影响[J].环境科学学报,34(9):2346-2354
Zhang W,Zhu X,Jin X,et al.2017.Evidence for organic phosphorus activation and transformation at the sediment-water interface during plant debris decomposition[J].Science of the Total Environment,583:458-465
Zhang R,Wu F,He Z,et al.2009.Phosphorus composition in sediments from seven different trophic lakes,China:A phosphorus-31 NMR study[J].Journal of Environmental Quality,38(1):353-359
Zhang T,Wang X,Jin X.2007.Variations of alkaline phosphatase activity and P fractions in sediments of a shallow Chinese eutrophic lake (Lake Taihu)[J].Environmental Pollution,150(2):288-294
Aspila K I,Agemian H,Chau A S Y.1976.A semi-automated method for the determination of inorganic,organic and total phosphate in sediments[J].Analyst,101(1200):187-197
Bowman R A.1989.A sequential extraction procedure with concentrated sulfuric acid and dilute base for soil organic phosphorus[J].Soil Science Society of America Journal,53(2):362-366
白秀玲,周云凯,李斌,等.2011.基于液相31P核磁共振分析的沉积物样品制备方法研究[J].环境科学,32(7):1980-1985
Bai X,Ding S,Fan C,et al.2009.Organic phosphorus species in surface sediments of a large,shallow,eutrophic lake,Lake Taihu,China[J].Environmental Pollution,157(8/9):2507-2513
Cade-Menun B,Liu C W.2014.Solution phosphorus-31 nuclear magnetic resonance spectroscopy of soils from 2005 to 2013:A review of sample preparation and experimental parameters[J].Soil Science Society of America Journal,78(1):19-37
Cade-Menun B J,Navaratnam J A,Walbridge M R.2006.Characterizing dissolved and particulate phosphorus in water with 31P nuclear magnetic resonance spectroscopy[J].Environmental Science & Technology,40(24):7874-7880
Cardoza L A,Korir A K,Otto W H,et al.2004.Applications of NMR spectroscopy in environmental science[J].Progress in Nuclear Magnetic Resonance Spectroscopy,45(3/4):209-238
Cade-Menun B J,Benitez-Nelson C R,Pellechia P,et al.2005.Refining 31P nuclear magnetic resonance spectroscopy for marine particulate samples:Storage conditions and extraction recovery[J].Marine Chemistry,97(3/4):293-306
Cade-Menun B J.2005.Characterizing phosphorus in environmental and agricultural samples by 31P nuclear magnetic resonance spectroscopy[J].Talanta,66(2):359-371
Ding S,Xu D,Bai X,et al.2013.Speciation of organic phosphorus in a sediment profile of Lake Taihu II.Molecular species and their depth attenuation[J].Journal of Environmental Sciences,25(5):925-932
Dittrich M,Chesnyuk A,Gudimov A,et al.2013.Phosphorus retention in a mesotrophic lake under transient loading conditions:insights from a sediment phosphorus binding form study[J].Water Research,47(3):1433-1447
Dell′Anno A,Danovaro R.2005.Extracellular DNA plays a key role in deep-sea ecosystem functioning[J].Science,309(5744):2179-2179
Huo S,Zan F,Xi B,et al.2011.Phosphorus fractionation in different trophic sediments of lakes from different regions,China[J].Journal of Environmental Monitoring,13(4):1088-1095
Huang Q,Wang Z,Wang C,et al.2005.Phosphorus release in response to pH variation in the lake sedimentswith different ratios of iron-bound P to calcium-bound P[J].Chemical Speciation & Bioavailability,17(2):55-61
Ishii Y,Harigae S,Tanimoto S,et al.2010.Spatial variation of phosphorus fractions in bottom sediments and the potential contributions to eutrophication in shallow lakes[J].Limnology,11(1):5-16
Jensen H S,Kristensen P,Jeppesen E,et al.1992.Iron:phosphorus ratio in surface sediment as an indicator of phosphate release from aerobic sediments in shallow lakes[M]//Sediment/Water Interactions.Springer,Dordrecht,731-743
Kovar J L,Pierzynski G M.2009.Methods of phosphorus analysis for soils,sediments,residuals,and waters second edition[J].Southern cooperative series bulletin,408
金相灿,王圣瑞,庞燕.2004.太湖沉积物磷形态及pH值对磷释放的影响[J].中国环境科学,24(6):707-711
K?iv T,N?ges T,Laas A.2011.Phosphorus retention as a function of external loading,hydraulic turnover time,area and relative depth in 54 lakes and reservoirs[J].Hydrobiologia,660(1):105-115
Kleeberg A,Herzog C,Hupfer M.2013.Redox sensitivity of iron in phosphorus binding does not impede lake restoration[J].Water Research,47(3):1491-1502
李军,刘丛强,王仕禄,等.2004.太湖五里湖表层沉积物中不同形态磷的分布特征[J].矿物学报,24(4):405-410
Liu J,Wang H,Yang H,et al.2009.Detection of phosphorus species in sediments of artificial landscape lakes in China by fractionation and phosphorus-31 nuclear magnetic resonance spectroscopy[J].Environmental Pollution,157(1):49-56
Meyers P A.1994.Preservation of elemental and isotopic source identification of sedimentary organic matter[J].Chemical Geology,114(3/4):289-302
Nyenje P M,Foppen J W,Uhlenbrook S,et al.2010.Eutrophication and nutrient release in urban areas of sub-Saharan Africa—A review[J].Science of the Total Environment,408(3):447-455
Paytan A,McLaughlin K.2007.The oceanic phosphorus cycle[J].Chemical Reviews,107(2):563-576
Paraskova J V,Sj?berg P J R,Rydin E.2014.Turnover of DNA-P and phospholipid-P in lake sediments[J].Biogeochemistry,119(1/3):361-370
钱轶超,陈英旭,楼莉萍,等.2010.核磁共振技术在沉积物磷素组分及迁移转化规律研究中的应用[J].应用生态学报,21(7):1892-1898
Reitzel K,Ahlgren J,DeBrabandere H,et al.2007.Degradation rates of organic phosphorus in lake sediment[J].Biogeochemistry,82(1):15-28
Ruttenberg K C,Goni M A.1997.Phosphorus distribution,C∶N∶P ratios,and δ13Coc in arctic,temperate,and tropical coastal sediments:tools for characterizing bulk sedimentary organic matter[J].Marine Geology,139(1/4):123-145
Shinohara R,Imai A,Kawasaki N,et al.2012.Biogenic phosphorus compounds in sediment and suspended particles in a shallow eutrophic lake:a 31P-nuclear magnetic resonance (31P NMR) study[J].Environmental Science & Technology,46(19):10572-10578
Simpson A J,Simpson M J,Soong R.2012.Nuclear magnetic resonance spectroscopy and its key role in environmental research[J].Environmental Science & Technology,46(21):11488-11496
Salas A M,Elliott E T,Westfall D G,et al.2003.The role of particulate organic matter in phosphorus cycling[J].Soil Science Society of America Journal,67(1):181-189
Smolen J M,Stone A T.1997.Divalent metal ion-catalyzed hydrolysis of phosphorothionate ester pesticides and their corresponding oxonates[J].Environmental Science & Technology,31(6):1664-1673
Turner B L,Mahieu N,Condron L M.2003.Phosphorus-31 nuclear magnetic resonance spectral assignments of phosphorus compounds in soil NaOH-EDTA extracts[J].Soil Science Society of America Journal,67(2):497-510
Turner B L,Cade-Menun B J,Condron L M,et al.2005.Extraction of soil organic phosphorus[J].Talanta,66(2):294-306
Wang Z,Liang Y,Kang W.2011.Utilization of dissolved organic phosphorus by different groups of phytoplankton taxa[J].Harmful Algae,12:113-118
Watts E E,Dean P A W,Martin R R.2002.31P nuclear magnetic resonance study of sediment microbial phospholipids[J].Canadian Journal of Analytical Sciences and Spectroscopy,47(5):127-133
Worsfold P J,Gimbert L J,Mankasingh U,et al.2005.Sampling,sample treatment and quality assurance issues for the determination of phosphorus species in natural waters and soils[J].Talanta,66(2):273-293
杨修,孙芳,任娜.2003.环青海湖地区生态环境问题及其治理对策[J].地域研究与开发,22(2):39-42
闫露霞,孙美平,姚晓军,等.2018.青藏高原湖泊水质变化及现状评价[J].环境科学学报,38(3):900-910
张文强,单保庆.2013.基于液相 31P 核磁共振(NMR)技术的巢湖沉积物中有机磷形态研究[J].环境科学学报,33(7):1967-1973
赵海超,王圣瑞,张莉,等.2014.有机质含量及其组分对洱海沉积物磷吸附-释放影响[J].环境科学学报,34(9):2346-2354
Zhang W,Zhu X,Jin X,et al.2017.Evidence for organic phosphorus activation and transformation at the sediment-water interface during plant debris decomposition[J].Science of the Total Environment,583:458-465
Zhang R,Wu F,He Z,et al.2009.Phosphorus composition in sediments from seven different trophic lakes,China:A phosphorus-31 NMR study[J].Journal of Environmental Quality,38(1):353-359
Zhang T,Wang X,Jin X.2007.Variations of alkaline phosphatase activity and P fractions in sediments of a shallow Chinese eutrophic lake (Lake Taihu)[J].Environmental Pollution,150(2):288-294
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