热带滨海植物红厚壳的抗逆生物学特性
|
摘要
为了解红厚壳(Calophyllum inophyllum)的抗逆特性,对西沙群岛自然生长的红厚壳叶片的形态解剖、生理生态、以及叶片和适生土壤的元素含量进行了研究。结果表明,红厚壳是阳生性植物,其上表皮厚,海绵组织发达且栅栏组织排列紧密,气孔排列松散且密度小(24.40 mm~(-2)),有利于叶片保水抵御干旱。叶片的叶绿素a、b含量低(分别为0.87和0.43 mg g~(-1)),表明红厚壳具适应强光环境的能力。叶片的MDA含量低(13.46 nmol g~(-1)),PRO含量高(127.89μg g~(-1)),SOD活性高(149.42 U g~(-1)),总抗氧化能力高(388.60 U g~(-1)),显示红厚壳能通过提高自身的抗氧化能力抵御膜脂过氧化伤害。红厚壳自然生长的珊瑚岛土壤较为贫瘠、营养元素含量低,但红厚壳植株体内具有较高的营养元素含量,表明红厚壳营养元素利用率高,对于贫瘠土壤具有很好的适应能力。因此,红厚壳具有较高的抗氧化胁迫能力和耐受干旱的能力,适宜生长在热带珊瑚岛等土壤贫瘠的生境,可以作为热带珊瑚岛防风固沙和植被恢复的工具种。
In order to understand the stress resistance of Calophyllum inophyllum, the morphological and anatomical, eco-physiological characteristics, element contents in leaves and soil of C. inophyllum growing in Xisha Islands were analyzed. The results showed that C. inophyllum was heliophyte plant with thick leaf,well-developed spongy tissue and tightly arranged palisade tissue with low stomata density(24.40 mm~(-2)), which was beneficial to its water retention and drought resistance of leaves. The contents of chlorophyll a and chlorophyll b were low(0.87 and 0.43 mg g~(-1), respectively), indicating that C. inophyllum had good adaptability to the high irradiation environment. The low level of malondialdehyde content(13.46 nmol g~(-1)), the high level of proline content(127.89 μg g~(-1)), and superoxide dismutase activity(149.42 U g~(-1)) in leaves indicated that it had strong antioxidant capacity(388.60 U g~(-1)), so as to alleviate membrane lipid peroxidation under stress conditions.Though the nutrient in natural growing soil was poor, the content of nutrient elements in leaves of C. inophyllum was high, indicated that it had high utilization and good adaptability to poor soil. Overall, C. inophyllum had good adaptability to strong radiation, drought and poor soil, and could be used as a tool species for rehabilitation of tropical coral islands.
In order to understand the stress resistance of Calophyllum inophyllum, the morphological and anatomical, eco-physiological characteristics, element contents in leaves and soil of C. inophyllum growing in Xisha Islands were analyzed. The results showed that C. inophyllum was heliophyte plant with thick leaf,well-developed spongy tissue and tightly arranged palisade tissue with low stomata density(24.40 mm~(-2)), which was beneficial to its water retention and drought resistance of leaves. The contents of chlorophyll a and chlorophyll b were low(0.87 and 0.43 mg g~(-1), respectively), indicating that C. inophyllum had good adaptability to the high irradiation environment. The low level of malondialdehyde content(13.46 nmol g~(-1)), the high level of proline content(127.89 μg g~(-1)), and superoxide dismutase activity(149.42 U g~(-1)) in leaves indicated that it had strong antioxidant capacity(388.60 U g~(-1)), so as to alleviate membrane lipid peroxidation under stress conditions.Though the nutrient in natural growing soil was poor, the content of nutrient elements in leaves of C. inophyllum was high, indicated that it had high utilization and good adaptability to poor soil. Overall, C. inophyllum had good adaptability to strong radiation, drought and poor soil, and could be used as a tool species for rehabilitation of tropical coral islands.
引文
[1]YANG X F,FANG J,TAO Z L.Analysis of the development and application value of Calophyllum inophyllum L.[J].Chin Wild Plant Res,2001,20(6):33-35.doi:10.3969/j.issn.1006-9690.2001.06.014.阳辛凤,方佳,陶忠良.海棠果的开发应用价值分析[J].中国野生植物资源,2001,20(6):33-35.doi:10.3969/j.issn.1006-9690.2001.06.014.
[2]TONG Y,JIAN S G,CHEN Q,et al.Vascular plant diversity of the Paracel Islands,China[J].Biodiv Sci,2013,21(3):364-374.doi:10.3724/SP.J.1003.2013.11222.童毅,简曙光,陈权,等.中国西沙群岛植物多样性[J].生物多样性,2013,21(3):364-374.doi:10.3724/SP.J.1003.2013.11222.
[3]TAO Z L,FANG J,LAI Q X.Distribution and growth characteristics of Calophyllum inophyllum L.in Hainan Island[J].Res Dev Mark,2003,19(2):85-87.doi:10.3969/j.issn.1005-8141.2003.02.009.陶忠良,方佳,赖齐贤.红厚壳在海南岛的分布及其生长习性[J].资源开发与市场,2003,19(2):85-87.doi:10.3969/j.issn.1005-8141.2003.02.009.
[4]JIA R F,YIN G T,YANG J C,et al.Research progress and application prospects on Calophyllum inophyllum L.[J].Guangdong For Sci Technol,2011,27(2):85-90.doi:10.3969/j.issn.1006-4427.2011.02.016.贾瑞丰,尹光天,杨锦昌,等.红厚壳的研究进展及应用前景[J].广东林业科技,2011,27(2):85-90.doi:10.3969/j.issn.1006-4427.2011.02.016.
[5]HAN C R,SONG X P,CHEN G Y.Advances in the studies on chemical components of Calophyllum and related pharmacological activities[J].Chin J Org Chem,2003,23(2):212-219.doi:10.3321/j.issn:0253-2786.2003.02.018.韩长日,宋小平,陈光英.红厚壳属植物化学成份及药理活性研究进展[J].有机化学,2003,23(2):212-219.doi:10.3321/j.issn:0253-2786.2003.02.018.
[6]CHEN J,TAO Z L.Empolder protective technique analysis of wild resource Calaphyllmn inophyllum Linn.[J].Res Dev Market,2007,23(11):1029-1031.doi:10.3969/j.issn.1005-8141.2007.11.022.陈娟,陶忠良.红厚壳野生资源的开发型保护途径分析[J].资源开发与市场,2007,23(11):1029-1031.doi:10.3969/j.issn.1005-8141.2007.11.022.
[7]BI H P,HAN C R,WANG M,et al.Studies on the fingerprint of Calophyllum membranaceum Gardn.et Champ[J].J Hannan Norm Univ(Nat Sci),2006,19(3):239-242.doi:10.3969/j.issn.1674-4942.2006.03.012.毕和平,韩长日,王媚,等.薄叶红厚壳的指纹图谱研究[J].海南师范学院学报(自然科学版),2006,19(3):239-242.doi:10.3969/j.issn.1674-4942.2006.03.012.
[8]HAN S Y,HAN C R.Extraction and determination of total alkaloids in Calophyllum inlophyllum Linn.by spectrophotography[J].Appl Chem Ind,2010,39(9):1419-1421,1431.doi:10.3969/j.issn.1671-3206.2010.09.043.韩淑云,韩长日.海南红厚壳中生物碱的提取与含量测定[J].应用化工,2010,39(9):1419-1421,1431.doi:10.3969/j.issn.1671-3206.2010.09.043.
[9]SAPUTRA E,NUGRAHA M W,HELWANI Z,et al.Synthesis of geopolymer from rice husk ash for biodiesel production of Calophyllum inophyllum seed oil[C/OL]//IOP Conference Series:Materials Science and Engineering.Bristol:IOP Publishing Ltd.,2018,345(1):012019.doi:10.1088/1757-899X/345/1/012019.
[10]LUCAS P W,CHOONG M F,TAN H T W,et al.The fracture toughness of the leaf of the dicotyledon Calophyllum inophyllum L.(Guttiferae)[J].Phil Trans Roy Soc Lond B:Biol Sci,1991,334(1269):95-106.doi:10.1098/rstb.1991.0099.
[11]LIU X T,GE C D,ZOU X Q,et al.Carbon,nitrogen geochemical characteristics and their implications on environmental change in the lagoon sediments of the Dongdao Island of Xisha Islands in South China Sea[J].Acta Oceanol Sin,2017,39(6):43-54.doi:10.3969/j.issn.0253-4193.2017.06.005.刘晓瞳,葛晨东,邹欣庆,等.西沙群岛东岛潟湖沉积物碳、氮元素地球化学特征及其指示的环境变化[J].海洋学报,2017,39(6):43-54.doi:10.3969/j.issn.0253-4193.2017.06.005.
[12]CAO L,ZHANG S F,SHI H Q,et al.Primarily observation of breeding population of great frigatebirds(Fregata minor)on Dong Island of Xisha Archipelago[J].Zool Res,2003,24(6):457-461.doi:10.3321/j.issn:0254-5853.2003.06.010.曹垒,张苏芳,史洪泉,等.西沙群岛东岛小军舰鸟繁殖种群的初步观察[J].动物学研究,2003,24(6):457-461.doi:10.3321/j.issn:0254-5853.2003.06.010.
[13]ZHANG S M,LIU H Y,CHENG J J,et al.Free-hand section techniques for clear observation of cell structures of millet(Setaria italica)and rice(Oyrza sativa)leaves[J].Genom Appl Biol,2015,34(7):1527-1530.doi:10.13417/j.gab.034.001527.张书敏,刘红云,程金金,等.快速徒手切片法观察谷子和水稻叶片显微结构[J].基因组学与应用生物学,2015,34(7):1527-1530.doi:10.13417/j.gab.034.001527.
[14]DUNCAN W G.Leaf angles,leaf area,and canopy photosynthesis[J].Crop Sci,1971,11(4):482-485.doi:10.2135/cropsci1971.0011183X001100040006x.
[15]REN H,PENG S L,SUN G C,et al.The ecological comparison of Psychotria rubra and Rhodomytrus tomentosa in south China[J].Chin J Plant Ecol,1997,21(4):386-392.任海,彭少麟,孙谷畴,等.广东中部两种常见灌木的生态学比较[J].植物生态学报,1997,21(4):386-392.
[16]SACK L,COWAN P D,JAIKUMAR N,et al.The‘hydrology’of leaves:Co-ordination of structure and function in temperate woody species[J].Plant Cell Environ,2003,26(8):1343-1356.doi:10.1046/j.0016-8025.2003.01058.x.
[17]HU B F,HUANG H L,JI Y Z,et al.Evaluation of the optimum concentration of chlorophyll extract for determination of chlorophyll content by spectrophotometry[J].Pratacult Sci,2018,35(8):1965-1974.doi:10.11829/j.issn.1001-0629.2017-0418.胡秉芬,黄华梨,季元祖,等.分光光度法测定叶绿素含量的提取液的适宜浓度[J].草业科学,2018,35(8):1965-1974.doi:10.11829/j.issn.1001-0629.2017-0418.
[18]LI H S.Principles and Techniques of Plant Physiological Biochemical Experiment[M].Beijing:Higher Education Press,2000:35-160.李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:35-160.
[19]AEBI H.Catalase in vitro[J].Methods Enzymol,1984,105:121-126.doi:10.1016/S0076-6879(84)05016-3.
[20]ZOU Q.Experiment Instruction of Plant Physiology[M].Beijing:China Agriculture Press,2000:121-131.邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:121-131.
[21]WANG Y,CHEN J W,DI X Y.A comparative study on the SOD,POD,MDA and dissoluble protein of six provenances of Chinese pine(Pinus tabulaeformis Carr.)under water stress[J].Ecol Environ,2011,20(10):1449-1453.doi:10.3969/j.issn.1674-5906.2011.10.011.王琰,陈建文,狄晓艳.水分胁迫下不同油松种源SOD、POD、MDA及可溶性蛋白比较研究[J].生态环境学报,2011,20(10):1449-1453.doi:10.3969/j.issn.1674-5906.2011.10.011.
[22]DENG J M,XIONG G S,YUAN X L,et al.Differences in SOD,POD,CAT activities and MDA content and their responses to high temperature stress at peak flowering stage in cotton lines with different tolerance to high temperature[J].Cotton Sci,2010,22(3):242-247.doi:10.3969/j.issn.1002-7807.2010.03.009.邓茳明,熊格生,袁小玲,等.棉花不同耐高温品系的SOD、POD、CAT活性和MDA含量差异及其对盛花期高温胁迫的响应[J].棉花学报,2010,22(3):242-247.doi:10.3969/j.issn.1002-7807.2010.03.009.
[23]BENZIE I F F,STRAIN J J.The ferric reducing ability of plasma(FRAP)as a measure of“antioxidant power”:The FRAP assay[J].Anal Biochem,1996,239(1):70-76.doi:10.1006/abio.1996.0292.
[24]LI X Y,ZHAO Q Q,LIU Y,et al.Determination of abscisic acid in crabapple by high performance liquid chromatography[J].N Hort,2018(4):138-144.doi:10.11937/bfyy.20172564.李晓颍,赵晴晴,刘月,等.海棠果实中脱落酸的高效液相色谱测定[J].北方园艺,2018(4):138-144.doi:10.11937/bfyy.20172564.
[25]SUN Q,HU J J.Research Technology of Plant Physiology[M].Yangling:Northwest Agriculture and Forestry University Press,2006:1-200.孙群,胡景江.植物生理学研究技术[M].杨凌:西北农林科技大学出版社,2006:1-200.
[26]BATES L S,WALDREN R P,TEARE I D.Rapid determination of free proline for water:Stress studies[J].Plant Soil,1973,39(1):205-207.doi:10.1007/BF00018060.
[27]LIU Q,LI Y,YAO H Y.Determination of total polyphenol in barley seed extracts by Folin-Ciocalteu colorimetry[J].Food Sci Technol,2007,32(4):175-177.doi:10.3969/j.issn.1005-9989.2007.04.052.刘清,李玉,姚惠源.Folin-Ciocalteu比色法测定大麦提取液中总多酚的含量[J].食品科技,2007,32(4):175-177.doi:10.3969/j.issn.1005-9989.2007.04.052.
[28]WANG C T,LONG R J,LIU W,et al.Relationships between soil carbon distribution and species diversity and community biomass at different alpine meadows[J].Resour Sci,2010,32(10):2022-2029.王长庭,龙瑞军,刘伟,等.高寒草甸不同群落类型土壤碳分布与物种多样性、生物量关系[J].资源科学,2010,32(10):2022-2029.
[29]CORNELISSEN J H C,LAVOREL S,GARNIER E,et al.A handbook of protocols for standardised and easy measurement of plant functional traits worldwide[J].Aust J Bot,2003,51(4):335-380.doi:10.1071/BT02124.
[30]COSTA-SAURA J M,MARTíNEZ-VILALTA J,TRABUCCO A,et al.Specific leaf area and hydraulic traits explain niche segregation along an aridity gradient in Mediterranean woody species[J].Perspect Plant Ecol Evol Syst,2016,21:23-30.doi:10.1016/j.ppees.2016.05.001.
[31]SHI G R,CHENG X L,LIU L,et al.Anatomical and water physiological plasticity of Grewia biloba var.parviflora leaf and secondary xylem[J].Chin J Appl Ecol,2006,17(10):1801-1806.doi:10.13287/j.1001-9332.2006.0356.史刚荣,程雪莲,刘蕾,等.扁担木叶片和次生木质部解剖和水分生理特征的可塑性[J].应用生态学报,2006,17(10):1801-1806.doi:10.13287/j.1001-9332.2006.0356.
[32]SONG G M,LIU N,JIAN S G,et al.Physiological and biological characteristics of Terminalia catappa[J].J Trop Subtrop Bot,2018,26(1):40-46.doi:10.11926/jtsb.3801.宋光满,刘楠,简曙光,等.榄仁树的生理和生物学特性[J].热带亚热带植物学报,2018,26(1):40-46.doi:10.11926/jtsb.3801.
[33]WANG X H,LIU N,REN H,et al.Ecological and biological characteristics of Pisonia grandis[J].Guihaia,2017,37(12):1489-1497.doi:10.11931/guihaia.gxzw201709003.王馨慧,刘楠,任海,等.抗风桐(Pisonia grandis)的生态生物学特征[J].广西植物,2017,37(12):1489-1497.doi:10.11931/guihaia.gxzw201709003.
[34]WU S H,CHEN H W,JIAN S G,et al.The biological characteristics of Cordia subcordata on tropical coral island in China[J].Ecol Sci,2017,36(6):57-63.doi:10.14108/j.cnki.1008-8873.2017.06.008.吴淑华,陈昊雯,简曙光,等.中国热带珊瑚岛橙花破布木(Cordia subcordata)的生物学特性[J].生态科学,2017,36(6):57-63.doi:10.14108/j.cnki.1008-8873.2017.06.008.
[35]KULKARNI M,SCHNEIDER B,RAVEH E,et al.Leaf anatomical characteristics and physiological responses to short-term drought in Ziziphus mauritiana(Lamk.)[J].Sci Hort,2010,124(3):316-322.doi:10.1016/j.scienta.2010.01.005.
[36]HAN T T,LIU N,SONG G M,et al.Eco-physiological characteristics of Morinda citrifolia[J].J Trop Subtrop Bot,2018,26(1):33-39.doi:10.11926/jtsb.3811.韩涛涛,刘楠,宋光满,等.海滨木巴戟的生理生态特征研究[J].热带亚热带植物学报,2018,26(1):33-39.doi:10.11926/jtsb.3811.
[37]SINGH S K,RAJA REDDY K.Regulation of photosynthesis,fluorescence,stomatal conductance and water-use efficiency of cowpea[Vigna unguiculata(L.)Walp.]under drought[J].J Photochem Photobiol B:Biol,2011,105(1):40-50.doi:10.1016/j.jphotobiol.2011.07.001.
[38]ZHANG Y,WANG D X,SONG B,et al.Leaf anatomical structure based drought resistance evaluation of 11 urban forest plants in Xining City[J].J NW Agric For Univ(Nat Sci),2014,42(8):86-92,98.doi:10.13207/j.cnki.jnwafu.2014.08.004.张义,王得祥,宋彬,等.基于叶片解剖结构的西宁市11种城市森林植物抗旱性评价[J].西北农林科技大学学报(自然科学版),2014,42(8):86-92,98.doi:10.13207/j.cnki.jnwafu.2014.08.004.
[39]ZHAO J,BAI J,PAN Q H,et al.Study on changes regularity of chlorophyll contents in different Sabina chinensis(L.)varieties(Lines)during drought stress[J].Chin Agric Sci Bull,2007,23(3):236-239.doi:10.3969/j.issn.1000-6850.2007.03.052.赵瑾,白金,潘青华,等.干旱胁迫下圆柏不同品种(系)叶绿素含量变化规律[J].中国农学通报,2007,23(3):236-239.doi:10.3969/j.issn.1000-6850.2007.03.052.
[40]WU S,ZHANG X Q,CAI Y.Effects of drought stress on chlorophyll contents and photosynthetic characteristics of cucumber seedlings[J].Chin Agric Sci Bull,2014,30(1):133-137.吴顺,张雪芹,蔡燕.干旱胁迫对黄瓜幼苗叶绿素含量和光合特性的影响[J].中国农学通报,2014,30(1):133-137.
[41]ZHANG H M,WANG M Y,HOU J H.Effects of drought strees upon the contents of hydrogen peroxide and MDA and the activities of SODand CAT in maize[J].J Inner Mongolia Inst Anim Husb,1993,14(4):92-95.张海明,王茅雁,候建华.干旱对玉米过氧化氢、MDA含量及SOD、CAT活性的影响[J].内蒙古农牧学院学报,1993,14(4):92-95.
[42]DOBRA J,MOTYKA V,DOBREV P,et al.Comparison of hormonal responses to heat,drought and combined stress in tobacco plants with elevated proline content[J].J Plant Physiol,2010,167(16):1360-1370.doi:10.1016/j.jplph.2010.05.013.
[43]PRYOR W A.Free Radicals in Biology[M].New York:Academic Press,1976:1-239.
[44]MOLLIER A,PELLERIN S.Maize root system growth and development as influenced by phosphorus deficiency[J].J Exp Bot,1999,50(333):487-497.
[45]LIU N,WU S H,GUO Q F,et al.Leaf nitrogen assimilation and partitioning differ among subtropical forest plants in response to canopy addition of nitrogen treatments[J].Sci Total Environ,2018,637-638:1026-1034.doi:10.1016/j.scitotenv.2018.05.060.
[46]XU B B,LIU N,REN H,et al.Stress resistance biological characteristics of Scaevola sericea in Paracel Islands[J].Guihaia,2018,38(10):1277-1285.doi:10.11931/guihaia.gxzw201711012.徐贝贝,刘楠,任海,等.西沙群岛草海桐的抗逆生物学特性[J].广西植物,2018,38(10):1277-1285.doi:10.11931/guihaia.gxzw201711012.
[2]TONG Y,JIAN S G,CHEN Q,et al.Vascular plant diversity of the Paracel Islands,China[J].Biodiv Sci,2013,21(3):364-374.doi:10.3724/SP.J.1003.2013.11222.童毅,简曙光,陈权,等.中国西沙群岛植物多样性[J].生物多样性,2013,21(3):364-374.doi:10.3724/SP.J.1003.2013.11222.
[3]TAO Z L,FANG J,LAI Q X.Distribution and growth characteristics of Calophyllum inophyllum L.in Hainan Island[J].Res Dev Mark,2003,19(2):85-87.doi:10.3969/j.issn.1005-8141.2003.02.009.陶忠良,方佳,赖齐贤.红厚壳在海南岛的分布及其生长习性[J].资源开发与市场,2003,19(2):85-87.doi:10.3969/j.issn.1005-8141.2003.02.009.
[4]JIA R F,YIN G T,YANG J C,et al.Research progress and application prospects on Calophyllum inophyllum L.[J].Guangdong For Sci Technol,2011,27(2):85-90.doi:10.3969/j.issn.1006-4427.2011.02.016.贾瑞丰,尹光天,杨锦昌,等.红厚壳的研究进展及应用前景[J].广东林业科技,2011,27(2):85-90.doi:10.3969/j.issn.1006-4427.2011.02.016.
[5]HAN C R,SONG X P,CHEN G Y.Advances in the studies on chemical components of Calophyllum and related pharmacological activities[J].Chin J Org Chem,2003,23(2):212-219.doi:10.3321/j.issn:0253-2786.2003.02.018.韩长日,宋小平,陈光英.红厚壳属植物化学成份及药理活性研究进展[J].有机化学,2003,23(2):212-219.doi:10.3321/j.issn:0253-2786.2003.02.018.
[6]CHEN J,TAO Z L.Empolder protective technique analysis of wild resource Calaphyllmn inophyllum Linn.[J].Res Dev Market,2007,23(11):1029-1031.doi:10.3969/j.issn.1005-8141.2007.11.022.陈娟,陶忠良.红厚壳野生资源的开发型保护途径分析[J].资源开发与市场,2007,23(11):1029-1031.doi:10.3969/j.issn.1005-8141.2007.11.022.
[7]BI H P,HAN C R,WANG M,et al.Studies on the fingerprint of Calophyllum membranaceum Gardn.et Champ[J].J Hannan Norm Univ(Nat Sci),2006,19(3):239-242.doi:10.3969/j.issn.1674-4942.2006.03.012.毕和平,韩长日,王媚,等.薄叶红厚壳的指纹图谱研究[J].海南师范学院学报(自然科学版),2006,19(3):239-242.doi:10.3969/j.issn.1674-4942.2006.03.012.
[8]HAN S Y,HAN C R.Extraction and determination of total alkaloids in Calophyllum inlophyllum Linn.by spectrophotography[J].Appl Chem Ind,2010,39(9):1419-1421,1431.doi:10.3969/j.issn.1671-3206.2010.09.043.韩淑云,韩长日.海南红厚壳中生物碱的提取与含量测定[J].应用化工,2010,39(9):1419-1421,1431.doi:10.3969/j.issn.1671-3206.2010.09.043.
[9]SAPUTRA E,NUGRAHA M W,HELWANI Z,et al.Synthesis of geopolymer from rice husk ash for biodiesel production of Calophyllum inophyllum seed oil[C/OL]//IOP Conference Series:Materials Science and Engineering.Bristol:IOP Publishing Ltd.,2018,345(1):012019.doi:10.1088/1757-899X/345/1/012019.
[10]LUCAS P W,CHOONG M F,TAN H T W,et al.The fracture toughness of the leaf of the dicotyledon Calophyllum inophyllum L.(Guttiferae)[J].Phil Trans Roy Soc Lond B:Biol Sci,1991,334(1269):95-106.doi:10.1098/rstb.1991.0099.
[11]LIU X T,GE C D,ZOU X Q,et al.Carbon,nitrogen geochemical characteristics and their implications on environmental change in the lagoon sediments of the Dongdao Island of Xisha Islands in South China Sea[J].Acta Oceanol Sin,2017,39(6):43-54.doi:10.3969/j.issn.0253-4193.2017.06.005.刘晓瞳,葛晨东,邹欣庆,等.西沙群岛东岛潟湖沉积物碳、氮元素地球化学特征及其指示的环境变化[J].海洋学报,2017,39(6):43-54.doi:10.3969/j.issn.0253-4193.2017.06.005.
[12]CAO L,ZHANG S F,SHI H Q,et al.Primarily observation of breeding population of great frigatebirds(Fregata minor)on Dong Island of Xisha Archipelago[J].Zool Res,2003,24(6):457-461.doi:10.3321/j.issn:0254-5853.2003.06.010.曹垒,张苏芳,史洪泉,等.西沙群岛东岛小军舰鸟繁殖种群的初步观察[J].动物学研究,2003,24(6):457-461.doi:10.3321/j.issn:0254-5853.2003.06.010.
[13]ZHANG S M,LIU H Y,CHENG J J,et al.Free-hand section techniques for clear observation of cell structures of millet(Setaria italica)and rice(Oyrza sativa)leaves[J].Genom Appl Biol,2015,34(7):1527-1530.doi:10.13417/j.gab.034.001527.张书敏,刘红云,程金金,等.快速徒手切片法观察谷子和水稻叶片显微结构[J].基因组学与应用生物学,2015,34(7):1527-1530.doi:10.13417/j.gab.034.001527.
[14]DUNCAN W G.Leaf angles,leaf area,and canopy photosynthesis[J].Crop Sci,1971,11(4):482-485.doi:10.2135/cropsci1971.0011183X001100040006x.
[15]REN H,PENG S L,SUN G C,et al.The ecological comparison of Psychotria rubra and Rhodomytrus tomentosa in south China[J].Chin J Plant Ecol,1997,21(4):386-392.任海,彭少麟,孙谷畴,等.广东中部两种常见灌木的生态学比较[J].植物生态学报,1997,21(4):386-392.
[16]SACK L,COWAN P D,JAIKUMAR N,et al.The‘hydrology’of leaves:Co-ordination of structure and function in temperate woody species[J].Plant Cell Environ,2003,26(8):1343-1356.doi:10.1046/j.0016-8025.2003.01058.x.
[17]HU B F,HUANG H L,JI Y Z,et al.Evaluation of the optimum concentration of chlorophyll extract for determination of chlorophyll content by spectrophotometry[J].Pratacult Sci,2018,35(8):1965-1974.doi:10.11829/j.issn.1001-0629.2017-0418.胡秉芬,黄华梨,季元祖,等.分光光度法测定叶绿素含量的提取液的适宜浓度[J].草业科学,2018,35(8):1965-1974.doi:10.11829/j.issn.1001-0629.2017-0418.
[18]LI H S.Principles and Techniques of Plant Physiological Biochemical Experiment[M].Beijing:Higher Education Press,2000:35-160.李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:35-160.
[19]AEBI H.Catalase in vitro[J].Methods Enzymol,1984,105:121-126.doi:10.1016/S0076-6879(84)05016-3.
[20]ZOU Q.Experiment Instruction of Plant Physiology[M].Beijing:China Agriculture Press,2000:121-131.邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:121-131.
[21]WANG Y,CHEN J W,DI X Y.A comparative study on the SOD,POD,MDA and dissoluble protein of six provenances of Chinese pine(Pinus tabulaeformis Carr.)under water stress[J].Ecol Environ,2011,20(10):1449-1453.doi:10.3969/j.issn.1674-5906.2011.10.011.王琰,陈建文,狄晓艳.水分胁迫下不同油松种源SOD、POD、MDA及可溶性蛋白比较研究[J].生态环境学报,2011,20(10):1449-1453.doi:10.3969/j.issn.1674-5906.2011.10.011.
[22]DENG J M,XIONG G S,YUAN X L,et al.Differences in SOD,POD,CAT activities and MDA content and their responses to high temperature stress at peak flowering stage in cotton lines with different tolerance to high temperature[J].Cotton Sci,2010,22(3):242-247.doi:10.3969/j.issn.1002-7807.2010.03.009.邓茳明,熊格生,袁小玲,等.棉花不同耐高温品系的SOD、POD、CAT活性和MDA含量差异及其对盛花期高温胁迫的响应[J].棉花学报,2010,22(3):242-247.doi:10.3969/j.issn.1002-7807.2010.03.009.
[23]BENZIE I F F,STRAIN J J.The ferric reducing ability of plasma(FRAP)as a measure of“antioxidant power”:The FRAP assay[J].Anal Biochem,1996,239(1):70-76.doi:10.1006/abio.1996.0292.
[24]LI X Y,ZHAO Q Q,LIU Y,et al.Determination of abscisic acid in crabapple by high performance liquid chromatography[J].N Hort,2018(4):138-144.doi:10.11937/bfyy.20172564.李晓颍,赵晴晴,刘月,等.海棠果实中脱落酸的高效液相色谱测定[J].北方园艺,2018(4):138-144.doi:10.11937/bfyy.20172564.
[25]SUN Q,HU J J.Research Technology of Plant Physiology[M].Yangling:Northwest Agriculture and Forestry University Press,2006:1-200.孙群,胡景江.植物生理学研究技术[M].杨凌:西北农林科技大学出版社,2006:1-200.
[26]BATES L S,WALDREN R P,TEARE I D.Rapid determination of free proline for water:Stress studies[J].Plant Soil,1973,39(1):205-207.doi:10.1007/BF00018060.
[27]LIU Q,LI Y,YAO H Y.Determination of total polyphenol in barley seed extracts by Folin-Ciocalteu colorimetry[J].Food Sci Technol,2007,32(4):175-177.doi:10.3969/j.issn.1005-9989.2007.04.052.刘清,李玉,姚惠源.Folin-Ciocalteu比色法测定大麦提取液中总多酚的含量[J].食品科技,2007,32(4):175-177.doi:10.3969/j.issn.1005-9989.2007.04.052.
[28]WANG C T,LONG R J,LIU W,et al.Relationships between soil carbon distribution and species diversity and community biomass at different alpine meadows[J].Resour Sci,2010,32(10):2022-2029.王长庭,龙瑞军,刘伟,等.高寒草甸不同群落类型土壤碳分布与物种多样性、生物量关系[J].资源科学,2010,32(10):2022-2029.
[29]CORNELISSEN J H C,LAVOREL S,GARNIER E,et al.A handbook of protocols for standardised and easy measurement of plant functional traits worldwide[J].Aust J Bot,2003,51(4):335-380.doi:10.1071/BT02124.
[30]COSTA-SAURA J M,MARTíNEZ-VILALTA J,TRABUCCO A,et al.Specific leaf area and hydraulic traits explain niche segregation along an aridity gradient in Mediterranean woody species[J].Perspect Plant Ecol Evol Syst,2016,21:23-30.doi:10.1016/j.ppees.2016.05.001.
[31]SHI G R,CHENG X L,LIU L,et al.Anatomical and water physiological plasticity of Grewia biloba var.parviflora leaf and secondary xylem[J].Chin J Appl Ecol,2006,17(10):1801-1806.doi:10.13287/j.1001-9332.2006.0356.史刚荣,程雪莲,刘蕾,等.扁担木叶片和次生木质部解剖和水分生理特征的可塑性[J].应用生态学报,2006,17(10):1801-1806.doi:10.13287/j.1001-9332.2006.0356.
[32]SONG G M,LIU N,JIAN S G,et al.Physiological and biological characteristics of Terminalia catappa[J].J Trop Subtrop Bot,2018,26(1):40-46.doi:10.11926/jtsb.3801.宋光满,刘楠,简曙光,等.榄仁树的生理和生物学特性[J].热带亚热带植物学报,2018,26(1):40-46.doi:10.11926/jtsb.3801.
[33]WANG X H,LIU N,REN H,et al.Ecological and biological characteristics of Pisonia grandis[J].Guihaia,2017,37(12):1489-1497.doi:10.11931/guihaia.gxzw201709003.王馨慧,刘楠,任海,等.抗风桐(Pisonia grandis)的生态生物学特征[J].广西植物,2017,37(12):1489-1497.doi:10.11931/guihaia.gxzw201709003.
[34]WU S H,CHEN H W,JIAN S G,et al.The biological characteristics of Cordia subcordata on tropical coral island in China[J].Ecol Sci,2017,36(6):57-63.doi:10.14108/j.cnki.1008-8873.2017.06.008.吴淑华,陈昊雯,简曙光,等.中国热带珊瑚岛橙花破布木(Cordia subcordata)的生物学特性[J].生态科学,2017,36(6):57-63.doi:10.14108/j.cnki.1008-8873.2017.06.008.
[35]KULKARNI M,SCHNEIDER B,RAVEH E,et al.Leaf anatomical characteristics and physiological responses to short-term drought in Ziziphus mauritiana(Lamk.)[J].Sci Hort,2010,124(3):316-322.doi:10.1016/j.scienta.2010.01.005.
[36]HAN T T,LIU N,SONG G M,et al.Eco-physiological characteristics of Morinda citrifolia[J].J Trop Subtrop Bot,2018,26(1):33-39.doi:10.11926/jtsb.3811.韩涛涛,刘楠,宋光满,等.海滨木巴戟的生理生态特征研究[J].热带亚热带植物学报,2018,26(1):33-39.doi:10.11926/jtsb.3811.
[37]SINGH S K,RAJA REDDY K.Regulation of photosynthesis,fluorescence,stomatal conductance and water-use efficiency of cowpea[Vigna unguiculata(L.)Walp.]under drought[J].J Photochem Photobiol B:Biol,2011,105(1):40-50.doi:10.1016/j.jphotobiol.2011.07.001.
[38]ZHANG Y,WANG D X,SONG B,et al.Leaf anatomical structure based drought resistance evaluation of 11 urban forest plants in Xining City[J].J NW Agric For Univ(Nat Sci),2014,42(8):86-92,98.doi:10.13207/j.cnki.jnwafu.2014.08.004.张义,王得祥,宋彬,等.基于叶片解剖结构的西宁市11种城市森林植物抗旱性评价[J].西北农林科技大学学报(自然科学版),2014,42(8):86-92,98.doi:10.13207/j.cnki.jnwafu.2014.08.004.
[39]ZHAO J,BAI J,PAN Q H,et al.Study on changes regularity of chlorophyll contents in different Sabina chinensis(L.)varieties(Lines)during drought stress[J].Chin Agric Sci Bull,2007,23(3):236-239.doi:10.3969/j.issn.1000-6850.2007.03.052.赵瑾,白金,潘青华,等.干旱胁迫下圆柏不同品种(系)叶绿素含量变化规律[J].中国农学通报,2007,23(3):236-239.doi:10.3969/j.issn.1000-6850.2007.03.052.
[40]WU S,ZHANG X Q,CAI Y.Effects of drought stress on chlorophyll contents and photosynthetic characteristics of cucumber seedlings[J].Chin Agric Sci Bull,2014,30(1):133-137.吴顺,张雪芹,蔡燕.干旱胁迫对黄瓜幼苗叶绿素含量和光合特性的影响[J].中国农学通报,2014,30(1):133-137.
[41]ZHANG H M,WANG M Y,HOU J H.Effects of drought strees upon the contents of hydrogen peroxide and MDA and the activities of SODand CAT in maize[J].J Inner Mongolia Inst Anim Husb,1993,14(4):92-95.张海明,王茅雁,候建华.干旱对玉米过氧化氢、MDA含量及SOD、CAT活性的影响[J].内蒙古农牧学院学报,1993,14(4):92-95.
[42]DOBRA J,MOTYKA V,DOBREV P,et al.Comparison of hormonal responses to heat,drought and combined stress in tobacco plants with elevated proline content[J].J Plant Physiol,2010,167(16):1360-1370.doi:10.1016/j.jplph.2010.05.013.
[43]PRYOR W A.Free Radicals in Biology[M].New York:Academic Press,1976:1-239.
[44]MOLLIER A,PELLERIN S.Maize root system growth and development as influenced by phosphorus deficiency[J].J Exp Bot,1999,50(333):487-497.
[45]LIU N,WU S H,GUO Q F,et al.Leaf nitrogen assimilation and partitioning differ among subtropical forest plants in response to canopy addition of nitrogen treatments[J].Sci Total Environ,2018,637-638:1026-1034.doi:10.1016/j.scitotenv.2018.05.060.
[46]XU B B,LIU N,REN H,et al.Stress resistance biological characteristics of Scaevola sericea in Paracel Islands[J].Guihaia,2018,38(10):1277-1285.doi:10.11931/guihaia.gxzw201711012.徐贝贝,刘楠,任海,等.西沙群岛草海桐的抗逆生物学特性[J].广西植物,2018,38(10):1277-1285.doi:10.11931/guihaia.gxzw201711012.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |