甘蓝型油菜DH群体主要品质性状相关性及主成分分析
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摘要
研究甘蓝型油菜品质性状之间的相关性,以期有效改良油菜的品质性状。将170份DH纯系于2009—2012年进行田间试验,并对油菜籽粒品质进行检测。同时,对油菜籽粒品质表型进行相关性和主成分分析。结果表明,父本和母本芥酸含量的变异系数最大(相差15.53%)。油菜DH群体中硫甙含量的变异幅度最大(128.43μmol/g饼粕),饱和脂肪酸的变异幅度最小(1.97%)。DH群体籽粒品质性状表型变异表现出超亲分离的遗传特点。除了芥酸含量,含油量与其他组分呈现出极显著的负相关,特别是与蛋白质含量的负相关最大(R=-0.72);蛋白质含量与油酸、亚油酸、亚麻酸和硫甙含量均呈极显著的正相关,与含油量和芥酸呈极显著的负相关;除了蛋白质含量,其他品质含量皆与硫甙含量呈现出极显著或者不显著负相关。主成分分析可将品质性状综合为油酸亚油酸酸因子、蛋白质因子、亚麻酸因子和硫甙因子,累积贡献率达93.94%,基本反映甘蓝型油菜品质性状的全面信息。
The aim is to investigate the relationship among quality traits of Brassica napus for their improvement. In total, 170 DH lines were tested in field experiments for three consecutive years(2009-2012),and the grain quality was detected. The correlations and principal components of quality traits were used to analyze quality phenotype data of DH lines. The results showed that: the variation coefficient of the erucic acid content was the biggest between the female and male parent(differing by 15.53%). The glucosinolate content in DH population had the largest variation range(128.43 μmol/g), and the variation range of saturated fatty acids content was the smallest(1.97%). The quality phenotypic variation in DH population showed genetic characteristics of transgression. Except erucic acid content, oil content showed a significant negative correlation with other quality traits, among which, the negative correlation with protein content was the largest(R=-0.72).The protein content showed extremely and significantly positive correlation with oleic acid, linoleic acid,linolenic acid and glucosinolate content, but it demonstrated extremely negative correlation with oil content and erucic acid content. Except protein content, other quality traits content showed extremely and significantly or not significantly negative correlation with glucosinolate content. Through the principal component analysis, the rapeseed quality traits could be integrated into oleic acid linoleic acid, protein, linolenic acid and glucosinolate factor, with the total cumulative contribution rate of 93.94%, which basically reflects the full information of the quality traits in Brassica napus.
The aim is to investigate the relationship among quality traits of Brassica napus for their improvement. In total, 170 DH lines were tested in field experiments for three consecutive years(2009-2012),and the grain quality was detected. The correlations and principal components of quality traits were used to analyze quality phenotype data of DH lines. The results showed that: the variation coefficient of the erucic acid content was the biggest between the female and male parent(differing by 15.53%). The glucosinolate content in DH population had the largest variation range(128.43 μmol/g), and the variation range of saturated fatty acids content was the smallest(1.97%). The quality phenotypic variation in DH population showed genetic characteristics of transgression. Except erucic acid content, oil content showed a significant negative correlation with other quality traits, among which, the negative correlation with protein content was the largest(R=-0.72).The protein content showed extremely and significantly positive correlation with oleic acid, linoleic acid,linolenic acid and glucosinolate content, but it demonstrated extremely negative correlation with oil content and erucic acid content. Except protein content, other quality traits content showed extremely and significantly or not significantly negative correlation with glucosinolate content. Through the principal component analysis, the rapeseed quality traits could be integrated into oleic acid linoleic acid, protein, linolenic acid and glucosinolate factor, with the total cumulative contribution rate of 93.94%, which basically reflects the full information of the quality traits in Brassica napus.
引文
[1]沈金雄,傅廷栋,涂金星,等.中国油菜生产以及遗传改良潜力与油菜生物柴油发展前景[J].华中农业大学学报,2007,12(6):894-899.
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[14]王鹏,李贵全.不同大豆杂交后代新品系产量性状的主成分分析[J].山西农业大学学报:自然科学版,2012,32(5):408-411.
[15]张晓杰,姜慧芳,任小平,等.中国花生核心种质的主成分分析及相关分析[J].中国油料作物学报,2009,31(3):298-304.
[16]白志英,李存东,孙红春,等.小麦代换系抗旱生理指标的主成分分析及综合评价[J].中国农业科学,2008,41(12):4264-4272.
[17]Shin E C,Huang C E,Lee B W,et al.Chemometric Approach to Fatty Acid Profiles in Soybean Cultivars by Principal Component Analysis(PCA)[J].Prev Nutr Food Sci,2012,17(3):184-191.
[18]张建模,邹小云,宋来强,等.杂交油菜主要产量性状与品质性状的关系研究[J].江西农业学报,2006,18(6):16-20.
[19]朱惠琴,张宪银,薛庆中.烟草两个DH群体农艺性状的遗传分析[J].浙江大学学报:农业与生命科学版,2004,30(5):477-481.
[20]余凤群,金明源,肖才升,等.甘蓝型油菜DH群体几个数量性状的遗传分析[J].中国农业科学,1998,31(3):44-48.
[21]Zhao J Y,Becker H C,Zhang D Q,et al.Conditional QTL mapping of oil content in rapeseed with respect to protein content and traits related to plant development and grain yield[J].Theor Appl Genet,2006,113(1):33-38.
[22]王竹云,杨翠玲.核磁共振(NMR)测量油菜籽含油量的应用[J].西部粮油科技,2000,25(6):55-57.
[23]Burns M J,Barnes S R,Bowman J G,et al.QTL analysis of an intervarietal set of substitution lines in Brassica napus:(i)Seed oil content and fatty acid composition[J].Heredity,2003,90(1):39-48.
[24]唐启义,冯明光.数据分析与DPS系统[M].北京:科学出版社,2002.
[25]Wang X F,Liu G H,Yang Q,et al.Genetic analysis on oil content in rapeseed(Brassica napus L.)[J].Euphytica,2010,173:17-24.
[26]涂进东,石云,吴建国,等.蛋白质和脂肪含量对油菜籽品质性状间相关性的影响[J].中国油料学报,2009,24(2):82-88.
[27]胡良平.现代统计学与SAS应用[M].军事医学科学出版社,2002:316-323.
[28]Si P,Mailer R J,Galwey N,et al.Influence of genotype and environment on oil and protein concentrations of canola(Brassica napus L.)grown across southern Australia[J].Crop and Pasture Science,2003,54(4):397-407.
[29]Zhao J Y,Becker H C,Zhang D Q,et al.Oil content in a European×Chinese rapeseed population:QTL with additive and epistatic effects and their genotype-environment interactions[J].Crop Science,2005,45(1):51-59.
[2]李爱民,周德银,惠飞虎,等.大籽粒优质甘蓝型油菜新品种扬油9号的选育[J].江苏农业科学,2014,42(2):78-79.
[3]Basunanda P,Radoev M,Ecke W,et al.Comparative mapping of quantitative trait loci involved in heterosis for seedling and yield traits in oilseed rape(Brassica napus L.)[J].Theoretical and Applied Genetics,2010,120:271-281.
[4]王汉中,殷艳.中国油料产业形势分析与发展对策建议[J].中国油料作物学报,2014,36(3):414-421.
[5]Broun P,Shanklin J,Whittle E,et al.Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids[J].Science,1998,282:1315-1317.
[6]Nagano Y,Arai H,Kita T.High density lipoprotein loses its effect to stimulate efflux of cholesterol from foam cells after oxidative modification[J].Proceedings of the National Academy of Sciences,1991,88(15):6457-6461.
[7]Velasco L,Becker H C.Estimating the fatty acid composition of the oil in intact-seed rapeseed(Brassica napus L.)by near-infrared reflectance spectroscopy[J].Euphytica,1998,101:221-230.
[8]韩锦峰,董钻.作物生物化学[M].北京:中国农业出版社,1995:198-203,243-247.
[9]白志英,李存东,孙红春.小麦代换系抗旱生理指标的主成分分析及综合评价[J].中国农业科学,2008,41(12):4264-4272.
[10]孟庆立.关周博,冯佰利,等.胡银岗谷子抗旱相关性状的主成分与模糊聚类分析[J].中国农业科学,2009,42(8):2667-2675.
[11]朱宗河,郑文寅,张学昆.甘蓝型油菜耐旱相关性状的主成分分析及综合评价[J].中国农业科学,2011,44(9):l775-l787.
[12]邹德堂.黑龙江省稻米品质性状的主成分分析[J].东北农业大学学报,2008,39(3):17-21.
[13]孙宪印,吴科,钱兆国.黄淮冬麦区北片水地供试小麦品种(系)主要品质性状的主成分分析和聚类分析[J].山东农业科学,2006(1):24-26.
[14]王鹏,李贵全.不同大豆杂交后代新品系产量性状的主成分分析[J].山西农业大学学报:自然科学版,2012,32(5):408-411.
[15]张晓杰,姜慧芳,任小平,等.中国花生核心种质的主成分分析及相关分析[J].中国油料作物学报,2009,31(3):298-304.
[16]白志英,李存东,孙红春,等.小麦代换系抗旱生理指标的主成分分析及综合评价[J].中国农业科学,2008,41(12):4264-4272.
[17]Shin E C,Huang C E,Lee B W,et al.Chemometric Approach to Fatty Acid Profiles in Soybean Cultivars by Principal Component Analysis(PCA)[J].Prev Nutr Food Sci,2012,17(3):184-191.
[18]张建模,邹小云,宋来强,等.杂交油菜主要产量性状与品质性状的关系研究[J].江西农业学报,2006,18(6):16-20.
[19]朱惠琴,张宪银,薛庆中.烟草两个DH群体农艺性状的遗传分析[J].浙江大学学报:农业与生命科学版,2004,30(5):477-481.
[20]余凤群,金明源,肖才升,等.甘蓝型油菜DH群体几个数量性状的遗传分析[J].中国农业科学,1998,31(3):44-48.
[21]Zhao J Y,Becker H C,Zhang D Q,et al.Conditional QTL mapping of oil content in rapeseed with respect to protein content and traits related to plant development and grain yield[J].Theor Appl Genet,2006,113(1):33-38.
[22]王竹云,杨翠玲.核磁共振(NMR)测量油菜籽含油量的应用[J].西部粮油科技,2000,25(6):55-57.
[23]Burns M J,Barnes S R,Bowman J G,et al.QTL analysis of an intervarietal set of substitution lines in Brassica napus:(i)Seed oil content and fatty acid composition[J].Heredity,2003,90(1):39-48.
[24]唐启义,冯明光.数据分析与DPS系统[M].北京:科学出版社,2002.
[25]Wang X F,Liu G H,Yang Q,et al.Genetic analysis on oil content in rapeseed(Brassica napus L.)[J].Euphytica,2010,173:17-24.
[26]涂进东,石云,吴建国,等.蛋白质和脂肪含量对油菜籽品质性状间相关性的影响[J].中国油料学报,2009,24(2):82-88.
[27]胡良平.现代统计学与SAS应用[M].军事医学科学出版社,2002:316-323.
[28]Si P,Mailer R J,Galwey N,et al.Influence of genotype and environment on oil and protein concentrations of canola(Brassica napus L.)grown across southern Australia[J].Crop and Pasture Science,2003,54(4):397-407.
[29]Zhao J Y,Becker H C,Zhang D Q,et al.Oil content in a European×Chinese rapeseed population:QTL with additive and epistatic effects and their genotype-environment interactions[J].Crop Science,2005,45(1):51-59.
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