遮阴胁迫对麦冬和多年生黑麦草根际土壤细菌群落结构和多样性的影响
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摘要
为探究麦冬(Ophiopogon japonicus (Linn.f.)Ker-Gawl.,缩写为OJ)和多年生黑麦草(Lolium perenne L.,缩写为LP)根际土壤微生物群落对遮阴胁迫的响应机制,本试验利用Illumina HiSeq2500测序平台,以16SrRNA基因为标靶进行相关研究。结果表明:遮阴改变OJ和LP土壤中硝态氮、铵态氮、全磷、全钾、速效钾和速效磷含量;遮阴显著改变OJ和LP根际土壤细菌群落组成,但是对其多样性影响不显著;OJ和LP根际土壤中变形菌门(Proteobacteria)和酸杆菌门(Acidobacteria)为主要优势菌群,相对丰度分别为26.34%～35.23%,19.79%～24.24%和19.03%～24.49%,19.20%～32.96%。遮阴导致OJ根际土壤中变形菌门、绿弯菌门(Chloroflexi)和疣微菌门(Verrucomicrobia)的丰度降低,而LP根际土壤中其相对丰度显著高于OJ。相关性分析表明:土壤速效钾、全氮是影响OJ根际土壤细菌群落结构的主要原因,而铵态氮、硝态氮、全氮是影响LP根际土壤细菌群落结构的主要原因。因此可得出,遮阴改变了OJ和LP土壤的理化性质,改变了其根际土壤中优势细菌类群组成,而植物本身对土壤细菌群落组成的影响强于遮阴胁迫。
An experiment was conducted to examine the impact of shade stress on soil microbial community structure and diversity in the rhizosphere of shade-tolerant Ophiopogon japonicus(Linn.f.)Ker-Gawl.(OJ)and shade-intolerant perennial turf-type ryegrasss(Lolium perenne L.,LP).Bacterial community structure was assayed using high-throughput sequencing platform,targeting the 16 SrRNA gene.The results showed that shade stress changed the contents of NH4+-N and NO3--N,total P,total K,available K,and available P in the rhizosphere soil of OJ and LP.Shade stress significantly changed the composition of bacterial communities,while had little impact on the diversity of the OJ and LP's microbial communities.The bacterial communities were mostly composed of Proteobacteria and Acidobacteria in the rhizosphere soil of OJ and LP.The relative abundances of Proteobacteria and Acidobacteria in the rhizosphere soil of OJ and LP were 26.34%～35.23%,19.79%～24.24%and 19.03%～24.49%,19.20%～32.96%,respectively.The relative abundances of Chloroflexi and Verrucomicrobia decreased in OJ soil in response to shade stress,and higher abundances was observed in LP soil compared to OJ soil.The correlation analysis indicated that available K and total N were major factors affecting the bacterial assemblages in the rhizosphere soil of OJ,while total N,NH4+-N and NO3--N concentration had strong effect on bacterial assemblages in the rhizosphere soil of LP.In conclusion,shade stress changed the soil physicochemical properties and bacterial community structures;plants per se have stronger impacts on soil bacterial community than shade stress.
An experiment was conducted to examine the impact of shade stress on soil microbial community structure and diversity in the rhizosphere of shade-tolerant Ophiopogon japonicus(Linn.f.)Ker-Gawl.(OJ)and shade-intolerant perennial turf-type ryegrasss(Lolium perenne L.,LP).Bacterial community structure was assayed using high-throughput sequencing platform,targeting the 16 SrRNA gene.The results showed that shade stress changed the contents of NH4+-N and NO3--N,total P,total K,available K,and available P in the rhizosphere soil of OJ and LP.Shade stress significantly changed the composition of bacterial communities,while had little impact on the diversity of the OJ and LP's microbial communities.The bacterial communities were mostly composed of Proteobacteria and Acidobacteria in the rhizosphere soil of OJ and LP.The relative abundances of Proteobacteria and Acidobacteria in the rhizosphere soil of OJ and LP were 26.34%～35.23%,19.79%～24.24%and 19.03%～24.49%,19.20%～32.96%,respectively.The relative abundances of Chloroflexi and Verrucomicrobia decreased in OJ soil in response to shade stress,and higher abundances was observed in LP soil compared to OJ soil.The correlation analysis indicated that available K and total N were major factors affecting the bacterial assemblages in the rhizosphere soil of OJ,while total N,NH4+-N and NO3--N concentration had strong effect on bacterial assemblages in the rhizosphere soil of LP.In conclusion,shade stress changed the soil physicochemical properties and bacterial community structures;plants per se have stronger impacts on soil bacterial community than shade stress.
引文
[1]尹淑霞．几种冷季型草坪草的耐荫性研究[D]．兰州：甘肃农业大学，2000:1-2
[2]杨燕，杨晓华，孙彦．不同遮荫强度对草地早熟禾草坪质量的影响[J]．草地学报，2010,18(3):447-451
[3] Hussain S,Iqbal N,Brestic M,et al.Changes in morphology,chlorophyll fluorescence performance and Rubisco activity of soybean in response to foliar application of ionic titanium under normal light and shade environment[J].Science of the Total Environment,2019,658:626-637
[4] Jiang C D,Wang X,Gao H Y,et al.Systemic regulation of leaf anatomical structure,photosynthetic performance,and highlight tolerance in sorghum[J].Plant Physiology,2011,155(3):1416-1424
[5] Fan Y,Chen J,Wang Z,et al.Soybean(Glycine max L.Merr.)seedlings response to shading:leaf structure,photosynthesis and proteomic analysis[J].BMC Plant Biology,2019,19(1):34
[6]王思思，蔡捡，刘金平，等．基质和遮阴对结缕草冷季生长幼苗构件及生物量分配的影响[J]．草地学报，2016,24(6):1296-1303
[7] Zhang X H,Liu Q,Liu Y H,et al.Nitric oxide is involved in abscisic acid-induced photosynthesis and antioxidant system of tall fescue seedlings response to low-light stress[J].Environmental and Experimental Botany,2018,155:226-238
[8] Li L,Li X Y,Xu X W,et al.Assimilative branches and leaves of the desert plant Alhagi sparsifolia Shap.possesses a different adaptation mechanism to shade[J].Plant Physiology and Biochemistry,2014,74:239-245
[9] Wang C,Zheng M M,Hu A Y,et al.Diazotroph abundance and community composition in an acidic soil in response to aluminum-tolerant and aluminum-sensitive maize(Zea mays L.)cultivars under two nitrogen fertilizer forms[J].Plant and Soil,2018,424(1-2):463-478
[10]尚浩博，殷宪强，吉普辉．环境土壤学实验[M]．咸阳：西北农林科技大学出版社，2017:6-34
[11]Sun W M,Li J W,Lei J,et al.Profiling microbial community structures across six large oilfields in China and the potential role of dominant microorganisms in bioremediation[J].Applied Microbiology and Biotechnology,2015,99(20):8751-8764
[12]Magoc T,Salzberg S.FLASH:Fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963
[13]Edgar R C,Haas B J,Clemente J C,et al.UCHIME improves sensitivity and speed of chimera detection[J].Bioinformatics,2011,27(16):2194-2200
[14]Edgar R C.UPARSE:Highly accurate OTU sequences from microbial amplicon reads[J].Nature Methods,2013,10(10):996-998
[15]Wang Q,Garrity G M,Tiedje J M,et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Apply Environmental Microbiology,2007,73(16):5261-5267
[16]崔佩佩，武爱莲，王劲松，等．不同施肥处理对高粱根际土壤微生物功能多样性的影响[J]．华北农学报，2018,33(05):195-202
[17]Acosta-Martínez V,Cotton J,Gardner T,et al.Predominant bacterial and fungal assemblages in agricultural soils during a record drought/heat wave and linkages to enzyme activities of biogeochemical cycling[J].Applied Soil Ecology,2014,84:69-82
[18]Tóth Z,Táncsics A,Kriszt B,et al.Extreme effects of drought on composition of the soil bacterial community and decomposition of plant tissue:bacterial community and plant tissue decomposition[J].European Journal of Soil Science,2017,68(4):504-513
[19]Yuste C,Fernandez-Gonzalez A J,Fernandez-Lopez M,et al.Strong functional stability of soil microbial communities under semiarid Mediterranean conditions and subjected to long-term shifts in baseline precipitation[J].Soil Biology and Biochemistry,2014,69:223-233
[20]Van Horn D J,Buelow H N,Gooseff M N,et al.Soil microbial responses to increased moisture and organic resources along a salinity gradient in a Polar Desert[J].Applied and Environmental Microbiology,2014,80(10):3034-3043
[21]Hartmann M,Brunner I,Hagedorn F,et al.A decade of irrigation transforms the soil microbiome of a semi-arid pine forest[J].Molecular Ecology,2017,26(4):1190-1206
[22]Frossard A,Donhauser J,Mestrot A,et al.Long and shortterm effects of mercury pollution on the soil microbiome[J].Soil Biology and Biochemistry,2018,120:191-199
[23]Kumar M,Mishra S,Dixit V,et al.Synergistic effect of Pseudomonas putida and Bacillus amyloliquefaciens ameliorates drought stress in chickpea(Cicer arietinum L.)[J].Plant Signaling&Behavior,2016,11(1):e1071004
[24]Etesami H,Maheshwari D K.Use of plant growth promoting rhizobacteria(PGPRs)with multiple plant growth promoting traits in stress agriculture:Action mechanisms and future prospects[J].Ecotoxicology and Environmental Safety,2018,156:225-246
[25]Bottomley P J,Taylor A E,Myrold D D.A consideration of the relative contributions of different microbial subpopulations to the soil N cycle[J].Frontiers in Microbiology,2012,3(373):373
[26]Boyle S A,Yarwood R R,Bottomley P J,et al.Bacterial and fungal contributions to soil nitrogen cycling under Douglas fir and red alder at two sites in Oregon[J].Soil Biology and Biochemistry,2008,40(2):443-451
[27]Nguyen L T T,Osanai Y,Lai KT,et al.Responses of the soil microbial community to nitrogen fertilizer regimes and historical exposure to extreme weather events:Flooding or prolongeddrought[J].Soil Biology and Biochemistry,2018,118:227-236
[28]Chodak M,Go??biewski M,Morawska-P?oskonka J,et al.Soil chemical properties affect the reaction of forest soil bacteria to drought and rewetting stress[J].Annals of Microbiology,2015,65:1627-1637
[2]杨燕，杨晓华，孙彦．不同遮荫强度对草地早熟禾草坪质量的影响[J]．草地学报，2010,18(3):447-451
[3] Hussain S,Iqbal N,Brestic M,et al.Changes in morphology,chlorophyll fluorescence performance and Rubisco activity of soybean in response to foliar application of ionic titanium under normal light and shade environment[J].Science of the Total Environment,2019,658:626-637
[4] Jiang C D,Wang X,Gao H Y,et al.Systemic regulation of leaf anatomical structure,photosynthetic performance,and highlight tolerance in sorghum[J].Plant Physiology,2011,155(3):1416-1424
[5] Fan Y,Chen J,Wang Z,et al.Soybean(Glycine max L.Merr.)seedlings response to shading:leaf structure,photosynthesis and proteomic analysis[J].BMC Plant Biology,2019,19(1):34
[6]王思思，蔡捡，刘金平，等．基质和遮阴对结缕草冷季生长幼苗构件及生物量分配的影响[J]．草地学报，2016,24(6):1296-1303
[7] Zhang X H,Liu Q,Liu Y H,et al.Nitric oxide is involved in abscisic acid-induced photosynthesis and antioxidant system of tall fescue seedlings response to low-light stress[J].Environmental and Experimental Botany,2018,155:226-238
[8] Li L,Li X Y,Xu X W,et al.Assimilative branches and leaves of the desert plant Alhagi sparsifolia Shap.possesses a different adaptation mechanism to shade[J].Plant Physiology and Biochemistry,2014,74:239-245
[9] Wang C,Zheng M M,Hu A Y,et al.Diazotroph abundance and community composition in an acidic soil in response to aluminum-tolerant and aluminum-sensitive maize(Zea mays L.)cultivars under two nitrogen fertilizer forms[J].Plant and Soil,2018,424(1-2):463-478
[10]尚浩博，殷宪强，吉普辉．环境土壤学实验[M]．咸阳：西北农林科技大学出版社，2017:6-34
[11]Sun W M,Li J W,Lei J,et al.Profiling microbial community structures across six large oilfields in China and the potential role of dominant microorganisms in bioremediation[J].Applied Microbiology and Biotechnology,2015,99(20):8751-8764
[12]Magoc T,Salzberg S.FLASH:Fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963
[13]Edgar R C,Haas B J,Clemente J C,et al.UCHIME improves sensitivity and speed of chimera detection[J].Bioinformatics,2011,27(16):2194-2200
[14]Edgar R C.UPARSE:Highly accurate OTU sequences from microbial amplicon reads[J].Nature Methods,2013,10(10):996-998
[15]Wang Q,Garrity G M,Tiedje J M,et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Apply Environmental Microbiology,2007,73(16):5261-5267
[16]崔佩佩，武爱莲，王劲松，等．不同施肥处理对高粱根际土壤微生物功能多样性的影响[J]．华北农学报，2018,33(05):195-202
[17]Acosta-Martínez V,Cotton J,Gardner T,et al.Predominant bacterial and fungal assemblages in agricultural soils during a record drought/heat wave and linkages to enzyme activities of biogeochemical cycling[J].Applied Soil Ecology,2014,84:69-82
[18]Tóth Z,Táncsics A,Kriszt B,et al.Extreme effects of drought on composition of the soil bacterial community and decomposition of plant tissue:bacterial community and plant tissue decomposition[J].European Journal of Soil Science,2017,68(4):504-513
[19]Yuste C,Fernandez-Gonzalez A J,Fernandez-Lopez M,et al.Strong functional stability of soil microbial communities under semiarid Mediterranean conditions and subjected to long-term shifts in baseline precipitation[J].Soil Biology and Biochemistry,2014,69:223-233
[20]Van Horn D J,Buelow H N,Gooseff M N,et al.Soil microbial responses to increased moisture and organic resources along a salinity gradient in a Polar Desert[J].Applied and Environmental Microbiology,2014,80(10):3034-3043
[21]Hartmann M,Brunner I,Hagedorn F,et al.A decade of irrigation transforms the soil microbiome of a semi-arid pine forest[J].Molecular Ecology,2017,26(4):1190-1206
[22]Frossard A,Donhauser J,Mestrot A,et al.Long and shortterm effects of mercury pollution on the soil microbiome[J].Soil Biology and Biochemistry,2018,120:191-199
[23]Kumar M,Mishra S,Dixit V,et al.Synergistic effect of Pseudomonas putida and Bacillus amyloliquefaciens ameliorates drought stress in chickpea(Cicer arietinum L.)[J].Plant Signaling&Behavior,2016,11(1):e1071004
[24]Etesami H,Maheshwari D K.Use of plant growth promoting rhizobacteria(PGPRs)with multiple plant growth promoting traits in stress agriculture:Action mechanisms and future prospects[J].Ecotoxicology and Environmental Safety,2018,156:225-246
[25]Bottomley P J,Taylor A E,Myrold D D.A consideration of the relative contributions of different microbial subpopulations to the soil N cycle[J].Frontiers in Microbiology,2012,3(373):373
[26]Boyle S A,Yarwood R R,Bottomley P J,et al.Bacterial and fungal contributions to soil nitrogen cycling under Douglas fir and red alder at two sites in Oregon[J].Soil Biology and Biochemistry,2008,40(2):443-451
[27]Nguyen L T T,Osanai Y,Lai KT,et al.Responses of the soil microbial community to nitrogen fertilizer regimes and historical exposure to extreme weather events:Flooding or prolongeddrought[J].Soil Biology and Biochemistry,2018,118:227-236
[28]Chodak M,Go??biewski M,Morawska-P?oskonka J,et al.Soil chemical properties affect the reaction of forest soil bacteria to drought and rewetting stress[J].Annals of Microbiology,2015,65:1627-1637
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