荧光量子点标记技术在生物医药领域的研究进展
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摘要
荧光量子点标记技术作为荧光标记技术领域的最为先进的技术,因其无放射污染性、低细胞毒性以及检测技术成熟等优点,已经成为现代生物医药领域研究中的不可或缺的手段和技术。本文从各类荧光量子点制备、表面功能化方面的最新技术进行详细论述,对其在生物医药领域的如细胞内外物质追踪、生物组织成像、疾病早期诊断等方面的研究趋势和应用前景进行了展望。
Fluorescent quantum dot labeling technology, as the most advanced technology in the field of fluorescent labeling technology, has become an indispensable means and technology in the research of modern biomedicine because of its advantages of no radioactive contamination, low cytotoxicity and mature detection technology. In this paper, the latest technologies in various quantum dot preparation and surface functionalization are discussed in detail, and the research trends and application prospects in the field of biomedicine, such as intracellular and extracellular material tracking, biological tissue imaging, early disease diagnosis, etc., are prospected.
Fluorescent quantum dot labeling technology, as the most advanced technology in the field of fluorescent labeling technology, has become an indispensable means and technology in the research of modern biomedicine because of its advantages of no radioactive contamination, low cytotoxicity and mature detection technology. In this paper, the latest technologies in various quantum dot preparation and surface functionalization are discussed in detail, and the research trends and application prospects in the field of biomedicine, such as intracellular and extracellular material tracking, biological tissue imaging, early disease diagnosis, etc., are prospected.
引文
[1]量子点：现状、机遇和挑战.浙大学报《2014研究前沿》发布暨科学家论坛上的报告.
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[5]Bao H F, Wang E K, Dong S J.One pot synthesis of CdTe nanocrystals and shape control of luminescent CdTe cysteine nanocomposites[J].Small, 2006, 2:476-480.
[6]Wu S M, Sun X J, Wang L L, et al.Singlet oxygengenerating from fluorescence probes based on denatured bovine serum albumin-conjugated CdTe quantum dots and photosensitizer Chlorine6[J].Journal of Nanoparticle Research, 2014, 16(11):1-9.
[7]Cui R, Liu H H, Xie H Y, et al.Living Yeast Cells as a Controllable Biosynthesizer for Fluorescent Quantum Dots[J].Advanced Functional Materials, 2010, 19(15):2359-2364.
[8]吴盛美.荧光量子点的生物合成方法研究进展[J].微生物学通报，2017,44(2):449-457.
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[14]Zhou M, Nakatan E, Luisa S, et al.Peptide-Labeled Quantum Dots for Imaging GPCRs in Whole Cells and as Single Molecules[J].Bioconjugate Chem.2007, 18:323-332.
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[22]Nafiujjaman M, Joon H, Kwak K S, et al.Synthesis of nitrogen-and chlorine-dopedgraphene quantum dots for cancer cell imaging[J]. J Nanosci N anotechnol, 2018, 18(6):3793-3799.
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[25]李萌，等.量子点技术快速检测HCV核心抗原的研究[J].国际检验医学杂志，2009,5(30):430-433.
[26]郭爱玲，等.CdS量子点制备与单增李斯特菌抗体偶联的研究[J].分析化学研究学报，2010,5(38):632-637.
[27]余皓，等.量子点标记免疫层析技术检测血液中的降钙素原[J].分析化学研究学报，2014,11(42):1592-1597.
[28]Hao Y, et, al.Dual-Quantum-Dots-Labeled Lateral Flow Strip Rapidly Quantifies Procalcitonin andC-reactive Protein[J].Nanoscale Research Letters, 2016, 11:167.
[29]He J H, et, al.CdSe/ZnS Quantum Dot-Labeled Lateral Flow Strips for Rapid and Quantitative Detection of Gastric Cancer Carbohydrate Antigen 72-4[J].Nanoscale Research Letters, 2016,11:138.
[2]Murray C B, Norris D J, Bawendi M G.Synthesis and characterization of nearly monodisperse CdE(E=sulfur, selenium,tellurium)semiconductor nanocrystallites[J]. Am.Chem.Soc, 1993,115:8706-8715.
[3]Peng Z A, Peng X G.Formation of high quality CdTe, CdSe,and Cd S nanocrystals using CdO as precursor[J].Am.Chem.Soc,2001, 123:183-184.
[4]Gaponik N, Talapin D V, Rogach A L, et al.Thiolcapping of Cd Te nanocrystals:An alternative to organometallic synthetic routes[J].Phys.Chem.B, 2002, 106:7177-7185.
[5]Bao H F, Wang E K, Dong S J.One pot synthesis of CdTe nanocrystals and shape control of luminescent CdTe cysteine nanocomposites[J].Small, 2006, 2:476-480.
[6]Wu S M, Sun X J, Wang L L, et al.Singlet oxygengenerating from fluorescence probes based on denatured bovine serum albumin-conjugated CdTe quantum dots and photosensitizer Chlorine6[J].Journal of Nanoparticle Research, 2014, 16(11):1-9.
[7]Cui R, Liu H H, Xie H Y, et al.Living Yeast Cells as a Controllable Biosynthesizer for Fluorescent Quantum Dots[J].Advanced Functional Materials, 2010, 19(15):2359-2364.
[8]吴盛美.荧光量子点的生物合成方法研究进展[J].微生物学通报，2017,44(2):449-457.
[9]Borovaya M, Pirko Y, Krupodorova T, et al.Biosynthesis of cadmium sulphide quantum dots by using Pleurotus ostreatus(Jacq.)P. Kumm[J].Biotechnology&Biotechnological Equipment, 2015, 29(6):1-8.
[10]Bruchez M, Moronne M, Gin P, et al.Semiconductor nanocrystals as fluorescent biological labels[J].Science, 1998, 281:2013-2016.
[11]Chan W C W, Nie S M.Quantum dot bioconjugates for ultrasensitive nonisotopic detection[J]. Science, 1998, 281:2016-2018.
[12]Alivisatos A P, Gu W W, Larabell C.Quantum dots as cellular probes[J]. annual Review of Biomedical Engineering, 2005,7:55-76.
[13]Gao X H, Yang L, Nie S M, et al.In vivo molecular and cellular imaging with quantum dots[J], Curr.Opin.Biotechnol.2005,16:63-72.
[14]Zhou M, Nakatan E, Luisa S, et al.Peptide-Labeled Quantum Dots for Imaging GPCRs in Whole Cells and as Single Molecules[J].Bioconjugate Chem.2007, 18:323-332.
[15]Pavel Z, Mark S, Gao X H.Designing multifunctional quantum dots for bioimaging, detection, and drug delivery[J], Chem.Soc.Rev.2010, 39:4326-4354.
[16]Yang J, Shi V R, Gao X H, et al.Quantum dot nanobarcodes:epitaxial assembly of nanoparticle-polymer complexes in homogeneous solution[J].Am.Chem.Soc., 2008, 130:5286-5292.
[17]Zhou C H.Synthesis of size-tunable photoluminescent aqueous CdSe/ZnS microspheres via a phase transfer method with amphiphilic oligomer and their application for detection of HCG antigen[J].Mater.Chem., 2011, 21:7393-7400.
[18]周长华，王欣，等.聚合物-CdSe/ZnS量子点复合薄膜的制备及其荧光性能研究[J].无机材料学报，2010,7(25):700-704.
[19]吴家锴.量子点标记抗体探针的纯化和在肿瘤标志物检测中的应用[D].武汉大学，2016.
[20]Zrazhevskiy P, Gao X H.Quantum dot imaging platform for single-cell molecular profiling, Nature Communications[J].doi:10.1038/ncomms2635, 2013.
[21]张炎，等.Zn3In2S6-CEA量子点荧光探针对结肠癌细胞株SW480靶向标记及生物毒性研究[J].中华肿瘤防治杂志，2018,5(9):615-672.
[22]Nafiujjaman M, Joon H, Kwak K S, et al.Synthesis of nitrogen-and chlorine-dopedgraphene quantum dots for cancer cell imaging[J]. J Nanosci N anotechnol, 2018, 18(6):3793-3799.
[23]Akerman M E, Chan W C W, Laakkonen P, et al.Nanocrystal targeting in vivo[J].Sciences Applied Biological, 2002,99:12617.
[24]Gao X H, Nie S M, et al.In vivo cancer targeting and imaging with semiconductor quantum dots[J].Nat.Biotechnol., 2004,22:969-976.
[25]李萌，等.量子点技术快速检测HCV核心抗原的研究[J].国际检验医学杂志，2009,5(30):430-433.
[26]郭爱玲，等.CdS量子点制备与单增李斯特菌抗体偶联的研究[J].分析化学研究学报，2010,5(38):632-637.
[27]余皓，等.量子点标记免疫层析技术检测血液中的降钙素原[J].分析化学研究学报，2014,11(42):1592-1597.
[28]Hao Y, et, al.Dual-Quantum-Dots-Labeled Lateral Flow Strip Rapidly Quantifies Procalcitonin andC-reactive Protein[J].Nanoscale Research Letters, 2016, 11:167.
[29]He J H, et, al.CdSe/ZnS Quantum Dot-Labeled Lateral Flow Strips for Rapid and Quantitative Detection of Gastric Cancer Carbohydrate Antigen 72-4[J].Nanoscale Research Letters, 2016,11:138.
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