蝉棒束孢（蝉花）不同提取物抑制小鼠肥胖的比较
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摘要
蝉棒束孢(蝉花)Isariacicadae是药食两用真菌,具有多种生理活性,但其物质基础依然不明确。据此,本实验采用长期高脂高胆固醇饮食制备肥胖小鼠模型,灌胃给予蝉棒束孢乙醇提取物、水提取物和氢氧化钠碱水提取物等不同提取物,测定其血清中总胆固醇(T-CHO)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)等脂质代谢指标;观察其肝、肠组织的病理变化情况,以及肠道内容物中的菌群结构等,综合分析并筛选出蝉棒束孢改善肥胖的有效提取物或成分,为其进一步的深加工提供基础。结果表明,蝉棒束孢的不同溶剂提取物均可不同程度减缓高脂高胆固醇饮食诱导小鼠肥胖,同时改善其脂质代谢情况,减轻脂肪在肝脏中的堆积,减缓肠组织损伤,并调整肠道菌群结构,蝉棒束孢醇提取物效果较优,但其具体作用机制还有待进一步的研究揭示。
This study aims at screening the active extract from Isaria cicadae for controlling obesity and its complications. An obesity mouse model was prepared by feeding high fat and cholesterol diet to mice. Isaria cicadae extracts(ethyl alcohol extract, aqua destillata extract and alkaline water extract) were fed to the mice for 35 days by gavage. Histopathological changes of liver and colon, serum levels of total cholesterol(T-CHO), total triglycerides(TG), low-density lipoprotein cholesterol(LDL-C) and high-density lipoproteincholesterol(HDL-C) and gut microbiota were monitored. Results showed that all of the three extractions could reduce the body weight and improve the serum levels of T-CHO, TG, LDL-C and HDL-C of the experimental mice. The pathological observation revealed that the damages of liver and colon tissues induced by high fat and cholesterol diet were ameliorated more or less, and the alpha and beta diversity and gut microbiota composition were also improved. The ethyl alcohol extract of Isaria cicadae(ICE) has the best effect among the three tested samples, indicating that ICE would be the most active extract for obesity control while the functional mechanism remains to be studied.
This study aims at screening the active extract from Isaria cicadae for controlling obesity and its complications. An obesity mouse model was prepared by feeding high fat and cholesterol diet to mice. Isaria cicadae extracts(ethyl alcohol extract, aqua destillata extract and alkaline water extract) were fed to the mice for 35 days by gavage. Histopathological changes of liver and colon, serum levels of total cholesterol(T-CHO), total triglycerides(TG), low-density lipoprotein cholesterol(LDL-C) and high-density lipoproteincholesterol(HDL-C) and gut microbiota were monitored. Results showed that all of the three extractions could reduce the body weight and improve the serum levels of T-CHO, TG, LDL-C and HDL-C of the experimental mice. The pathological observation revealed that the damages of liver and colon tissues induced by high fat and cholesterol diet were ameliorated more or less, and the alpha and beta diversity and gut microbiota composition were also improved. The ethyl alcohol extract of Isaria cicadae(ICE) has the best effect among the three tested samples, indicating that ICE would be the most active extract for obesity control while the functional mechanism remains to be studied.
引文
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Cheng M,Zhang X,Zhu JY,Cheng L,Cao JX,Wu ZF,Weng PF,Zheng XJ,2018.Metagenomics approach to the intestinal microbiome structure and function in high fat diet-induced obesity mICS fed with oolong tea polyphenols.Food&Function,10:1039
Crovesy L,Ostrowski M,Ferreira DMTP,Rosado EL,Soares-Mota M,2017.Effect of Lactobacillus on body weight and body fat in overweight subjects:a systematic review of randomized controlled clinical trials.International Journal of Obesity,41(11):1607-1614
Dietz WH,2017.Obesity and excessive weight gain in young adults:new targets for prevention.Jama,318(3):241
Ding SL,Chi MM,Scull BP,Rigby R,Schwerbrock NMJ,Magness S,Jobin C,Lund PK,2010.High-fat diet:bacteria interactions promote intestinal inflammation which precedes and correlates with obesity and insulin resistance in mouse.PLoS One,5(8):e12191
Eckel N,Li YP,Kuxhaus O,Stefan N,Hu FB,Schulze MB,2018.Transition from metabolic healthy to unhealthy phenotypes and association with cardiovascular disease risk across BMI categories in 90 257 women(the Nurses'Health Study):30 year follow-up from a prospective cohort study.The Lancet Diabetes&Endocrinology,6(9):714-724
Ferry T,Laurent F,Ragois P,Chidiac C,Lyon BJI Study Group,2015.Post-traumatic chronic bone and joint infection caused by Butyricimonas spp.,and treated with high doses of ertapenem administered subcutaneously in a30-year-old obese man.BMJ Case Reports,pii:bcr2015212359
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Kondoh M,Shimada T,Fukada K,Morita M,Katada K,Higashimura Y,Mizushima K,Okamori M,Naito Y,Yoshikawa T,2014.Beneficial effects of heat-treated Enterococcus faecalis FK-23 on high-fat diet-induced hepatic steatosis in mice.British Journal of Nutrition,112(6):868-875
Langille MGI,Zaneveld,J,Caporaso,JG,McDonald D,Knights D,Reyes JA,Clemente JC,Burkepile DE,Thurber RLV,Knight R,Beiko RG,Huttenhower C,2013.Predictive functional profiling of microbial communities using 16SrRNA marker gene sequences.Nature Biotechnology,31(9):814-821
Lei H,Wang Y,Cai LL,Zhang F,2012.Preventive and curative effects and mechanism of sesamin against nonalcoholic fatty liver disease in mice.Food Science,33(23):331-335(in Chinese)
Ma RCW,2018.Epidemiology of diabetes and diabetic complications in China.Diabetologia,61(6):1249-1260
NCD Risk Factor Collaboration,2017.Worldwide trends in body-mass index,underweight,overweight,and obesity from 1975 to 2016:a pooled analysis of 2416population-based measurement studies in 128.9 million children,adolescents,and adults.The Lancet,390(10113):2627-2642
Nyberg ST,Batty GD,Pentti J,Virtanen M,Alfredsson L,Fransson EI,Goldberg M,Heikkil?K,Jokela M,Knutsson A,Koskenvuo M,Lallukka T,Leineweber C,Lindbohm JV,Madsen IEH,Hanson LLM,Nordin M,Oksanen T,Pietil?inen O,Rahkonen O,Rugulies R,Shipley MJ,Stenholm S,Suominen S,Theorell T,Vahtera J,Westerholm PJM,Westerlund H,Zins M,Hamer M,Singh-Manoux A,Bell JA,Ferrie JE,Kivim?ki M,2018.Obesity and loss of disease-free years owing to major non-communicable diseases:a multicohort study.The Lancet Public Health,3(10):e490-e497
Pang YJ,Kartsonaki C,Guo Y,Chen YP,Yang L,Bian Z,Bragg F,Millwood IY,Mao E,Li YL,Shi LY,Chen JS,Li LM,Holmes MV,Chen ZM,2018.Adiposity and risks of colorectal and small intestine cancer in Chinese adults:a prospective study of 0.5 million people.British Journal of Cancer,119(2):248
Profenno LA,Porsteinsson AP,Faraone SV,2010.Meta-analysis of Alzheimer’s disease risk with obesity,diabetes,and related disorders.Biological Psychiatry,67(6):505-512
Sichuan Food and Drug Administration,2011.Standards for Chinese medicinal materials in Sichuan Province.Sichuan Science and Technology Press,Chengdu.659-662(in Chinese)
Takahashi I,Kiyono H,1999.Gut as the largest immunologic tissue.Journal of Parenteral and Enteral Nutrition,23(5Suppl.):S7-S12
Thaiss CA,2018.Microbiome dynamics in obesity.Science,362(6417):903-904
Van Hul M,Geurts L,Plovier H,Druart C,Everard A,St?hlman M,Rhimi M,Chira K,Teissedre PL,Delzenne NM,Maguin E,Guilbot A,Brochot A,Gérard P,B?ckhed F,Cani PD,2018.Reduced obesity,diabetes,and steatosis upon cinnamon and grape pomace are associated with changes in gut microbiota and markers of gut barrier.American Journal of Physiology Endocrinology and Metabolism,314(4):E334-E352
Wunderlich CM,Ackermann PJ,Ostermann AL,Adams-Quack P,Vogt MC,Tran M,Nikolajev A,Waisman A,Garbers C,Theurich S,Mauer J,H?velmeyer N,Wunderlich FT,2018.Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment.Nature Communications,9:1646
Yu SJ,Chai XY,Fan MZ,2015.Analysis of nutritional and bioactive components of solid-state fermented substrate of Isaria cicadae.Food&Machinery,31(1):155-158(in Chinese)
Zhao LP,2013.The gut microbiota and obesity:from correlation to causality.Nature Reviews Microbiology,11(9):639
雷红，王毅，蔡亮亮，张凤，2012.芝麻素防治非酒精性脂肪肝作用及机理.食品科学，33(23):331-335
四川省食品药品监督管理局，2011.四川省中药材标准.成都：四川科学技术出版社.659-662
于士军，柴新义，樊美珍，2015.蝉花菌质主要营养成分和活性成分分析.食品与机械，31(1):155-158
Bruce-Keller AJ,Salbaum JM,Luo M,Blanchard E,Taylor CM,Welsh DA,Berthoud H,2015.Obese-type gut microbiota induce neurobehavioral changes in the absence of obesity.Biological Psychiatry,77(7):607-615
Chen DL,Yang X,Zheng CQ,Yang J,Chen J,Shuai O,Xie YZ,2017.Extracts from Hericium erinaceus relieve inflammatory bowel disease by regulating immunity and gut microbiota.Oncotarget,8(49):85838-85857
Cheng M,Zhang X,Zhu JY,Cheng L,Cao JX,Wu ZF,Weng PF,Zheng XJ,2018.Metagenomics approach to the intestinal microbiome structure and function in high fat diet-induced obesity mICS fed with oolong tea polyphenols.Food&Function,10:1039
Crovesy L,Ostrowski M,Ferreira DMTP,Rosado EL,Soares-Mota M,2017.Effect of Lactobacillus on body weight and body fat in overweight subjects:a systematic review of randomized controlled clinical trials.International Journal of Obesity,41(11):1607-1614
Dietz WH,2017.Obesity and excessive weight gain in young adults:new targets for prevention.Jama,318(3):241
Ding SL,Chi MM,Scull BP,Rigby R,Schwerbrock NMJ,Magness S,Jobin C,Lund PK,2010.High-fat diet:bacteria interactions promote intestinal inflammation which precedes and correlates with obesity and insulin resistance in mouse.PLoS One,5(8):e12191
Eckel N,Li YP,Kuxhaus O,Stefan N,Hu FB,Schulze MB,2018.Transition from metabolic healthy to unhealthy phenotypes and association with cardiovascular disease risk across BMI categories in 90 257 women(the Nurses'Health Study):30 year follow-up from a prospective cohort study.The Lancet Diabetes&Endocrinology,6(9):714-724
Ferry T,Laurent F,Ragois P,Chidiac C,Lyon BJI Study Group,2015.Post-traumatic chronic bone and joint infection caused by Butyricimonas spp.,and treated with high doses of ertapenem administered subcutaneously in a30-year-old obese man.BMJ Case Reports,pii:bcr2015212359
Kim KA,Gu W,Lee IA,Joh EH,Kim DH,2012.High fat diet-induced gut microbiota exacerbates inflammation and obesity in mICS via the TLR4 signaling pathway.PLoSOne,7(10):e47713
Kondoh M,Shimada T,Fukada K,Morita M,Katada K,Higashimura Y,Mizushima K,Okamori M,Naito Y,Yoshikawa T,2014.Beneficial effects of heat-treated Enterococcus faecalis FK-23 on high-fat diet-induced hepatic steatosis in mice.British Journal of Nutrition,112(6):868-875
Langille MGI,Zaneveld,J,Caporaso,JG,McDonald D,Knights D,Reyes JA,Clemente JC,Burkepile DE,Thurber RLV,Knight R,Beiko RG,Huttenhower C,2013.Predictive functional profiling of microbial communities using 16SrRNA marker gene sequences.Nature Biotechnology,31(9):814-821
Lei H,Wang Y,Cai LL,Zhang F,2012.Preventive and curative effects and mechanism of sesamin against nonalcoholic fatty liver disease in mice.Food Science,33(23):331-335(in Chinese)
Ma RCW,2018.Epidemiology of diabetes and diabetic complications in China.Diabetologia,61(6):1249-1260
NCD Risk Factor Collaboration,2017.Worldwide trends in body-mass index,underweight,overweight,and obesity from 1975 to 2016:a pooled analysis of 2416population-based measurement studies in 128.9 million children,adolescents,and adults.The Lancet,390(10113):2627-2642
Nyberg ST,Batty GD,Pentti J,Virtanen M,Alfredsson L,Fransson EI,Goldberg M,Heikkil?K,Jokela M,Knutsson A,Koskenvuo M,Lallukka T,Leineweber C,Lindbohm JV,Madsen IEH,Hanson LLM,Nordin M,Oksanen T,Pietil?inen O,Rahkonen O,Rugulies R,Shipley MJ,Stenholm S,Suominen S,Theorell T,Vahtera J,Westerholm PJM,Westerlund H,Zins M,Hamer M,Singh-Manoux A,Bell JA,Ferrie JE,Kivim?ki M,2018.Obesity and loss of disease-free years owing to major non-communicable diseases:a multicohort study.The Lancet Public Health,3(10):e490-e497
Pang YJ,Kartsonaki C,Guo Y,Chen YP,Yang L,Bian Z,Bragg F,Millwood IY,Mao E,Li YL,Shi LY,Chen JS,Li LM,Holmes MV,Chen ZM,2018.Adiposity and risks of colorectal and small intestine cancer in Chinese adults:a prospective study of 0.5 million people.British Journal of Cancer,119(2):248
Profenno LA,Porsteinsson AP,Faraone SV,2010.Meta-analysis of Alzheimer’s disease risk with obesity,diabetes,and related disorders.Biological Psychiatry,67(6):505-512
Sichuan Food and Drug Administration,2011.Standards for Chinese medicinal materials in Sichuan Province.Sichuan Science and Technology Press,Chengdu.659-662(in Chinese)
Takahashi I,Kiyono H,1999.Gut as the largest immunologic tissue.Journal of Parenteral and Enteral Nutrition,23(5Suppl.):S7-S12
Thaiss CA,2018.Microbiome dynamics in obesity.Science,362(6417):903-904
Van Hul M,Geurts L,Plovier H,Druart C,Everard A,St?hlman M,Rhimi M,Chira K,Teissedre PL,Delzenne NM,Maguin E,Guilbot A,Brochot A,Gérard P,B?ckhed F,Cani PD,2018.Reduced obesity,diabetes,and steatosis upon cinnamon and grape pomace are associated with changes in gut microbiota and markers of gut barrier.American Journal of Physiology Endocrinology and Metabolism,314(4):E334-E352
Wunderlich CM,Ackermann PJ,Ostermann AL,Adams-Quack P,Vogt MC,Tran M,Nikolajev A,Waisman A,Garbers C,Theurich S,Mauer J,H?velmeyer N,Wunderlich FT,2018.Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment.Nature Communications,9:1646
Yu SJ,Chai XY,Fan MZ,2015.Analysis of nutritional and bioactive components of solid-state fermented substrate of Isaria cicadae.Food&Machinery,31(1):155-158(in Chinese)
Zhao LP,2013.The gut microbiota and obesity:from correlation to causality.Nature Reviews Microbiology,11(9):639
雷红，王毅，蔡亮亮，张凤，2012.芝麻素防治非酒精性脂肪肝作用及机理.食品科学，33(23):331-335
四川省食品药品监督管理局，2011.四川省中药材标准.成都：四川科学技术出版社.659-662
于士军，柴新义，樊美珍，2015.蝉花菌质主要营养成分和活性成分分析.食品与机械，31(1):155-158
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