基于主成分分析法对耐盐石油降解菌生长因子的分析
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摘要
为了解决耐盐石油降解菌生物量不足的问题,以耐盐石油降解菌HDMP2为研究对象,鉴定其菌系归属,并采用单因素分析、因子分析和主成分分析方法分析其生长因子。结果表明,HDMP2菌株的5d柴油降解率为27.49%,通过BLAST比对和发育树分析,鉴定其为Halomonas sp.nmyj-1;当盐含量为5%、pH值为7.07、转速为160r·min~(-1)、接种量为2.5%、油含量为0.4%、培养时间为3d时,HDMP2菌株的生物量最高;6个生长因子对HDMP2菌株生物量的影响大小依次为转速、接种量、pH值、盐含量、油含量和培养时间。
In order to solve the problem of insufficient biomass of salt-tolerant petroleum degrading bacteria,using salt-tolerant petroleum degrading bacterium HDMP2 as a research object,we identified its classification and analyzed its growth factors by single-factor analysis,factor analysis,and principal component analysis.The results indicate that the diesel degradation rate of strain HDMP2 is 27.49%in 5 days,and it is identified as Halomonas sp.nmyj-1 by BLAST alignment and phylogenetic tree analysis.Moreover,the biomass of strain HDMP2 is the highest under the conditions as follows:the salt content of 5%,the pH value of 7.07,the rotation speed of 160 r·min~(-1),the inoculation amount of 2.5%,the oil content of 0.4%,and the culture time of3 d.Furthermore,the influence degree of 6 growth factors on the biomass of strain HDMP2 is in a following sequential order from the highest to the lowest:rotation speed,inoculation amount,pH value,salt content,oil content,and culture time.
In order to solve the problem of insufficient biomass of salt-tolerant petroleum degrading bacteria,using salt-tolerant petroleum degrading bacterium HDMP2 as a research object,we identified its classification and analyzed its growth factors by single-factor analysis,factor analysis,and principal component analysis.The results indicate that the diesel degradation rate of strain HDMP2 is 27.49%in 5 days,and it is identified as Halomonas sp.nmyj-1 by BLAST alignment and phylogenetic tree analysis.Moreover,the biomass of strain HDMP2 is the highest under the conditions as follows:the salt content of 5%,the pH value of 7.07,the rotation speed of 160 r·min~(-1),the inoculation amount of 2.5%,the oil content of 0.4%,and the culture time of3 d.Furthermore,the influence degree of 6 growth factors on the biomass of strain HDMP2 is in a following sequential order from the highest to the lowest:rotation speed,inoculation amount,pH value,salt content,oil content,and culture time.
引文
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[18] 龙训建,钱鞠,梁川.基于主成分分析的BP神经网络及其在需水预测中的应用[J].成都理工大学学报(自然科学版),2010,37(2):206-210.LONG X J,QIAN J,LIANG C.Water demand forecast model of BP neutral networks based on principle component analysis[J].Journal of Chengdu University of Technology(Natural Science Edition),2010,37(2):206-210.
[19] 赵拥军,陈景武,唐军.主成分分析与因子分析法在医院管理质量综合评价中的应用研究[J].中国卫生统计,2006,23(5):403-406.ZHAO Y J,CHEN J W,TANG J.An application research of synthetic evaluation about hospital management quality by using principle component analysis and factor analysis[J].Chinese Journal of Health Statistics,2006,23(5):403-406.
[20] 张喜娟,丁钊鹏.因子分析在学生成绩影响因素调查结果中的应用[J].北京联合大学学报(自然科学版),2010,24(1):77-81.ZHANG X J,DING Z P.The application of factor analysis to a study of student′s grades[J].Journal of Beijing Union University(Natural Science Edition),2010,24(1):77-81.
[2] WANG X,WANG X J,LIU M,et al.Adsorption-synergic biodegradation of diesel oil in synthetic seawater by acclimated strains immobilized on multifunctional materials[J].Marine Pollution Bulletin,2015,92(1/2):195-200.
[3] AL-MAILEM D M,SORKHOH N A,SALAMAH S,et al.Oilbioremediation potential of Arabian Gulf mud flats rich in diazotrophic hydrocarbon-utilizing bacteria[J].International Biodeterioration &Biodegradation,2010,64(3):218-225.
[4] BACHMANN R T,JOHNSON A C,EDYVEAN R G J.Biotechnology in the petroleum industry:an overview[J].International Biodeterioration &Biodegradation,2014,86(Part C):225-237.
[5] 吴涛,依艳丽,谢文军,等.产生物表面活性剂耐盐菌的筛选鉴定及其对石油污染盐渍化土壤的修复作用[J].环境科学学报,2013,33(12):3359-3367.WU T,YI Y L,XIE W J,et al.Isolation and identification of halotolerant biosurfactant-producing bacteria and its application in bioremediation of petroleum-contaminated saline soil[J].Acta Scientiae Circumstantiae,2013,33(12):3359-3367.
[6] 任立松.耐盐石油降解菌的分离鉴定及其降解特性研究[D].镇江:江苏科技大学,2014.REN L S.Isolation,identification of salt tolerant petroleum-degrading bacterium and degradation characteristics[D].Zhenjiang:Jiangsu University of Science and Technology,2014.
[7] 薛建良,刘志秀,吴亚男,等.海洋石油降解菌不同固定化方法的研究进展[J].科学技术与工程,2016,16(32):162-168,173.XUE J L,LIU Z X,WU Y N,et al.The research progress of immobilized petroleum degrading bacteria using different methods[J].Science Technology and Engineering,2016,16(32):162-168,173.
[8] LIN T C,YOUNG C C,HO M J,et al.Characterization of floating activity of indigenous diesel-assimilating bacterial isolates[J].Journal of Bioscience and Bioengineering,2005,99(5):466-472.
[9] SALMON C,CRABOS J L,SAMBUCO J P,et al.Artificial wetland performances in the purification efficiency of hydrocarbon wastewater[J].Water,Air,and Soil Pollution,1998,104(3/4):313-329.
[10] LIU Z X,XUE J L,WU Y N,et al.Preliminary analysis of dieseldegrading bacteria immobilized on organic carriers in seawater[J].China Petroleum Processing &Petrochemical Technology,2017,19(2):33-39.
[11] SENG P,DRANCOURT M,GOURIET F,et al.Ongoing revolution in bacteriology:routine identification of bacteria by matrixassisted laser desorption ionization time-of-flight mass spectrometry[J].Clinical Infectious Diseases,2009,49(4):543-551.
[12] 孔凡晶,王现洁.青藏高原盐湖嗜盐微生物资源及应用前景[J].科技导报,2017,35(12):27-31.KONG F J,WANG X J.Halophilic microorganisms in salt lakes on Tibet Plateau and their potential application[J].Science &Technology Review,2017,35(12):27-31.
[13] 王魁荣,张树军,李少贺,等.一株中度嗜盐菌Halomonas sp.NY-011的耐盐特性及机理[J].应用与环境生物学报,2010,16(2):256-260.WANG K R,ZHANG S J,LI S H,et al.Osmotolerance property and mechanism of a moderately halophilic bacterium Halomonas sp.NY-011[J].Chinese Journal of Applied and Environmental Biology,2010,16(2):256-260.
[14] 陈梅梅,钱文,田生,等.耐盐石油降解菌性能及降解条件优化[J].油气田环境保护,2012,22(3):7-9.CHEN M M,QIAN W,TIAN S,et al.Optimization of degradation properties and conditions of oil-degrading bacteria with salt tolerance[J].Environmental Protection of Oil &Gas Fields,2012,22(3):7-9.
[15] HE X X,WANG Y,WANG K M,et al.Research of the relationship of intracellular acidification and apoptosis in HeLa cells based on pH nanosensors[J].Science in China Series B:Chemistry,2007,50(2):258-265.
[16] 李海花,单爱琴,周海霞.石油降解菌的筛选及降解性能研究[J].污染防治技术,2008,21(5):10-12.LI H H,SHAN A Q,ZHOU H X.Study on the screening and function of degrading petroleum bacteria[J].Pollution Control Technology,2008,21(5):10-12.
[17] 万春燕,徐国良.福建省城市旅游竞争力评价研究[J].亚热带资源与环境学报,2011,6(1):73-77.WAN C Y,XU G L.On competitive ability of urban tourism in Fujian Province[J].Journal of Subtropical Resources and Environment,2011,6(1):73-77.
[18] 龙训建,钱鞠,梁川.基于主成分分析的BP神经网络及其在需水预测中的应用[J].成都理工大学学报(自然科学版),2010,37(2):206-210.LONG X J,QIAN J,LIANG C.Water demand forecast model of BP neutral networks based on principle component analysis[J].Journal of Chengdu University of Technology(Natural Science Edition),2010,37(2):206-210.
[19] 赵拥军,陈景武,唐军.主成分分析与因子分析法在医院管理质量综合评价中的应用研究[J].中国卫生统计,2006,23(5):403-406.ZHAO Y J,CHEN J W,TANG J.An application research of synthetic evaluation about hospital management quality by using principle component analysis and factor analysis[J].Chinese Journal of Health Statistics,2006,23(5):403-406.
[20] 张喜娟,丁钊鹏.因子分析在学生成绩影响因素调查结果中的应用[J].北京联合大学学报(自然科学版),2010,24(1):77-81.ZHANG X J,DING Z P.The application of factor analysis to a study of student′s grades[J].Journal of Beijing Union University(Natural Science Edition),2010,24(1):77-81.
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