螯合剂对鬼针草Cd、Zn、Pb和Cu吸收累积的影响
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摘要
[目的]探究螯合剂[乙二醇二乙醚二胺四乙酸(EGTA)、氨三乙酸(NTA)、乙二胺二琥珀酸(EDDS)]对鬼针草镉(Cd)、锌(Zn)、铅(Pb)和铜(Cu)吸收累积的影响,筛选出能够强化鬼针草重金属吸收能力的螯合剂。[方法]采用室内盆栽试验方法,在采自矿区的Cd、Zn、Pb和Cu复合污染的土壤上种植鬼针草,研究螯合剂EGTA、NTA和EDDS作用下鬼针草对污染土壤中Cd、Zn、Pb和Cu的吸收与转运。[结果]3种螯合剂均能促进鬼针草的生长,其促进效果为:EDDS>EGTA>NTA。从根茎叶组织单位含量看,EGTA、NTA和EDDS均能促进Zn在鬼针草茎部的吸收,促进Pb在鬼针草根部的吸收以及Cu在鬼针草根茎叶部的吸收。从植株吸收累积总量来看,EGTA促进鬼针草Zn吸收累积效果最好,EDDS促进鬼针草Pb、Cu吸收累积效果最好,3种螯合剂对鬼针草吸收Cd的影响效果不佳。EGTA作用下鬼针草叶部及茎部对Cu的富集系数和转移系数均高于对照,说明EGTA能有效促进Cu在鬼针草体内的富集及转运。[结论]除EGTA、EDDS对鬼针草吸收Cd影响作用不显著,且NTA不仅减少了鬼针草对Cd的吸收,对鬼针草Zn吸收提升也不显著外,3种螯合剂均显著提升了鬼针草对Zn、Pb和Cu的吸收累积量。
[Objective]To screen the effective chelator which can enhance the absorption of heavy metals by Bidenspilosa L.,the effects of chelators[glycol-bis-(2-aminoethylether)-N.N,N',N'-tetraacetic acid(ETGA),nitrilotriacetic acid(NTA)and ethylene diamine disuccinate(EDDS)]on the uptake and accumulation of Cd,Pb,Cu and Zn with Bidens pilosa L.were investigated.[Method]Pot experiments was conducted to evaluate the effects of application ETGA,NTA and EDDS on the uptake and transportation of heavy metals(Cd,Pb,Cu and Zn)from contaminated mining area soil with Bidenspilosa L..[Result]The results indicated that three chelators promoted the growth of Bidens pilosa L.,and the order of effects was EDDS>EGTA>NTA.Judging from the unit content of root,stem and leaf tissue,EGTA,NTA and EDDS could promote the absorption of Zn in stem,Pb in root and Cu in root,stem and leaf of Bidens pilosa L..Judging from the total absorption and accumulation of plants,EGTA had the best effect on absorption and accumulation of Zn,EDDS had the best effect on absorption and accumulation of Pb and Cu,and the effects of three chelators on absorption of Cd were not good in Bidens pilosa L..The accumulation and transfer coefficients of Cu in leaves and stems of Bidens pilosa L.treated with EGTA were higher than the control,indicating that EGTA could effectively promote the accumulation and transportation of Cu in Bidens pilosa L..[Conclusion]The chelators EGTA and EDDS did not significantly affect the absorption of Cd in Bidens pilosa L.,and NTA not only reduced the absorption of Cd in Bidens pilosa L.but also did not significantly increase the absorption of Zn.All three chelators significantly increased the accumulation of Zn,Pb and Cu in Bidens pilosa L..
[Objective]To screen the effective chelator which can enhance the absorption of heavy metals by Bidenspilosa L.,the effects of chelators[glycol-bis-(2-aminoethylether)-N.N,N',N'-tetraacetic acid(ETGA),nitrilotriacetic acid(NTA)and ethylene diamine disuccinate(EDDS)]on the uptake and accumulation of Cd,Pb,Cu and Zn with Bidens pilosa L.were investigated.[Method]Pot experiments was conducted to evaluate the effects of application ETGA,NTA and EDDS on the uptake and transportation of heavy metals(Cd,Pb,Cu and Zn)from contaminated mining area soil with Bidenspilosa L..[Result]The results indicated that three chelators promoted the growth of Bidens pilosa L.,and the order of effects was EDDS>EGTA>NTA.Judging from the unit content of root,stem and leaf tissue,EGTA,NTA and EDDS could promote the absorption of Zn in stem,Pb in root and Cu in root,stem and leaf of Bidens pilosa L..Judging from the total absorption and accumulation of plants,EGTA had the best effect on absorption and accumulation of Zn,EDDS had the best effect on absorption and accumulation of Pb and Cu,and the effects of three chelators on absorption of Cd were not good in Bidens pilosa L..The accumulation and transfer coefficients of Cu in leaves and stems of Bidens pilosa L.treated with EGTA were higher than the control,indicating that EGTA could effectively promote the accumulation and transportation of Cu in Bidens pilosa L..[Conclusion]The chelators EGTA and EDDS did not significantly affect the absorption of Cd in Bidens pilosa L.,and NTA not only reduced the absorption of Cd in Bidens pilosa L.but also did not significantly increase the absorption of Zn.All three chelators significantly increased the accumulation of Zn,Pb and Cu in Bidens pilosa L..
引文
[1]魏树和,杨传杰,周启星.三叶鬼针草等7种常见菊科杂草植物对重金属的超富集特征[J].环境科学,2008,29(10):2912-2918.
[2]高洁,周永刚,张琪,等.污泥耐受性草本植物的筛选及其对重金属Cu、Zn的富集[J].环境工程学报,2013,7(1):351-359.
[3]Saifullah,Meers E,Qadir M,et al.EDTA-assisted Pb phytoextraction[J].Chemosphere,2009,74(10):1279-1291.
[4]樊扬帆.外源螯合剂柠檬酸和NTA对苎麻修复重金属Cd污染土壤的研究[D].长沙:湖南大学,2015.
[5]张磊.螯合剂强化棉花对镉污染土壤修复的初步研究[J].水土保持学报,2015,29(4):321-326.
[6]樊扬帆,刘云国,龚小敏,等.外源螯合剂CA和NTA对苎麻修复铅镉复合污染土壤的影响[J].环境工程学报,2016,10(8):4547-4552.
[7]Najeeb U,Xu L,Ali S,et al.Citric acid enhances the phytoextraction of manganese and plant growth by alleviating the ultrastructural damages in Juncus effuses L.[J].Journal of Hazardous Materials,2009,170(2-3):1156-1163.
[8]景琪,李晔,张讓,等.螯合剂和商陆联合修复重金属Cd、Cu污染土壤的田间试验[J].武汉理工大学学报,2014,36(4):139-143.
[9]杨波,陈银萍,柯昀琪,等.EDDS对Cd胁迫下三叶鬼针草生长和抗氧化酶系统及Cd积累的影响[J].农业环境科学学报,2018,37(5):875-882.
[10]刘金,殷宪强,孙慧敏,等.EDDS与EDTA强化苎麻修复镉铅污染土壤[J].农业环境科学学报,2015,34(7):1293-1300.
[11]胡亚虎,魏树和,周启星,等.螯合剂在重金属污染土壤植物修复中的应用研究进展[J].农业环境科学学报,2010,29(11):2055-2063.
[12]Meers E,Tack F M G,Verloo M G.Degradability of ethylenediaminedisuccinicacid(EDDS)in metal contaiminated soils:Implications for its use soil remediation[J].Chemosphere,2008,70(3):358-363.
[13]Tandy S,Schulin R,Nowack B.Uptake of metals during chelantassisted phytoextraction with EDDS related to the solubilized metal concentration[J].Environmental Science&Technology,2006,40(8):2753-2758.
[14]Evangelou MWH,Bauer U,Ebel M,et al.The influence of EDDS and EDTA on the uptake of heavy metal of Cd and Cu from soil with tobacco Nicotiana tabacum[J].Chemosphere,2007,68(2):345-
353.
[15]熊国焕,潘义宏,何艳明,等.螯合剂对大口叶边草Pb、Cd、As吸收性影响研究[J].土壤,2012,44(2):282-289.
[16]Luo C L,Shen Z G,Li X D.Enhanced phytoextraction of Cu,Pb,Zn,and Cd with EDTA and EDDS[J].Chemosphere,2005,59(1):1-11.
[17]Tandy S,Schulin R,Nowack B.The influence of EDDS on the uptake of heavy metals in hydroponically grown sunflowers[J].Chemosphere,2008,62(9):1454-1463.
[18]Kayser A,Wenger K,Keller A,et al.Enhancement of phytoextration of Zn,Cd and Cu from calcareous soil:the use of NTA and sulfur amendments[J].Environmental Science&Technology,2000,34(9):1778-1783.
[19]钱猛,沈振国,魏岚.螯合剂EDDS和EDTA诱导海州香薷积累土壤重金属的比较研究[J].农业环境科学学报,2006,25(1):113-118.
[20]Santos F S,Hernandez-Allica J,Becerril J M,et al.Chelate-induced phytoextraction of metal polluted soils with Brachiaria decumbens[J].Chemosphere,2006,65(1):43-50.
[21]Liu D,Islam E,Li T Q,et al.Comparison of synthetic chelators and low molecular weight organic acids in enhancing phytoextraction of heavy metals by two ecotypes of Sedum alfredii Hance[J].Journal of Hazardous Materials,2008,153(1-2):114-122.
[22]魂魏岚,陈亚华,钱猛,等.可降解螯合剂EDDS诱导植物修复重金属污染土壤的潜力[J].南京农业大学学报,2006,29(2):33-38.
[23]Evangelou MWH,Ebel M,Schaeffer A.Chelate assisted phytoextraction of heavy metals from:Effect,mechanism,toxicity and fate of chelating agents[J].Chemosphere,2007,68(6):989-1103.
[24]Tandy S,Bossart K,Mueller R,et al.Extraction of heavy metals from soils using biodegradable chelating agents[J].Environmental Science and Technology,2004,38(3):937-944.
[25]Zhao L,Li T,Yu H,et al.Effects of[S,S]-ethylenediaminedisuccinic acid and nitrilotriacetic acid on the efficiency of Pb phytostabilization by Athyrium wardii(Hook.)grown in Pb-contaminated soils[J].Journal of Environmental Economics&Management,2016,182(1):94-100.
[2]高洁,周永刚,张琪,等.污泥耐受性草本植物的筛选及其对重金属Cu、Zn的富集[J].环境工程学报,2013,7(1):351-359.
[3]Saifullah,Meers E,Qadir M,et al.EDTA-assisted Pb phytoextraction[J].Chemosphere,2009,74(10):1279-1291.
[4]樊扬帆.外源螯合剂柠檬酸和NTA对苎麻修复重金属Cd污染土壤的研究[D].长沙:湖南大学,2015.
[5]张磊.螯合剂强化棉花对镉污染土壤修复的初步研究[J].水土保持学报,2015,29(4):321-326.
[6]樊扬帆,刘云国,龚小敏,等.外源螯合剂CA和NTA对苎麻修复铅镉复合污染土壤的影响[J].环境工程学报,2016,10(8):4547-4552.
[7]Najeeb U,Xu L,Ali S,et al.Citric acid enhances the phytoextraction of manganese and plant growth by alleviating the ultrastructural damages in Juncus effuses L.[J].Journal of Hazardous Materials,2009,170(2-3):1156-1163.
[8]景琪,李晔,张讓,等.螯合剂和商陆联合修复重金属Cd、Cu污染土壤的田间试验[J].武汉理工大学学报,2014,36(4):139-143.
[9]杨波,陈银萍,柯昀琪,等.EDDS对Cd胁迫下三叶鬼针草生长和抗氧化酶系统及Cd积累的影响[J].农业环境科学学报,2018,37(5):875-882.
[10]刘金,殷宪强,孙慧敏,等.EDDS与EDTA强化苎麻修复镉铅污染土壤[J].农业环境科学学报,2015,34(7):1293-1300.
[11]胡亚虎,魏树和,周启星,等.螯合剂在重金属污染土壤植物修复中的应用研究进展[J].农业环境科学学报,2010,29(11):2055-2063.
[12]Meers E,Tack F M G,Verloo M G.Degradability of ethylenediaminedisuccinicacid(EDDS)in metal contaiminated soils:Implications for its use soil remediation[J].Chemosphere,2008,70(3):358-363.
[13]Tandy S,Schulin R,Nowack B.Uptake of metals during chelantassisted phytoextraction with EDDS related to the solubilized metal concentration[J].Environmental Science&Technology,2006,40(8):2753-2758.
[14]Evangelou MWH,Bauer U,Ebel M,et al.The influence of EDDS and EDTA on the uptake of heavy metal of Cd and Cu from soil with tobacco Nicotiana tabacum[J].Chemosphere,2007,68(2):345-
353.
[15]熊国焕,潘义宏,何艳明,等.螯合剂对大口叶边草Pb、Cd、As吸收性影响研究[J].土壤,2012,44(2):282-289.
[16]Luo C L,Shen Z G,Li X D.Enhanced phytoextraction of Cu,Pb,Zn,and Cd with EDTA and EDDS[J].Chemosphere,2005,59(1):1-11.
[17]Tandy S,Schulin R,Nowack B.The influence of EDDS on the uptake of heavy metals in hydroponically grown sunflowers[J].Chemosphere,2008,62(9):1454-1463.
[18]Kayser A,Wenger K,Keller A,et al.Enhancement of phytoextration of Zn,Cd and Cu from calcareous soil:the use of NTA and sulfur amendments[J].Environmental Science&Technology,2000,34(9):1778-1783.
[19]钱猛,沈振国,魏岚.螯合剂EDDS和EDTA诱导海州香薷积累土壤重金属的比较研究[J].农业环境科学学报,2006,25(1):113-118.
[20]Santos F S,Hernandez-Allica J,Becerril J M,et al.Chelate-induced phytoextraction of metal polluted soils with Brachiaria decumbens[J].Chemosphere,2006,65(1):43-50.
[21]Liu D,Islam E,Li T Q,et al.Comparison of synthetic chelators and low molecular weight organic acids in enhancing phytoextraction of heavy metals by two ecotypes of Sedum alfredii Hance[J].Journal of Hazardous Materials,2008,153(1-2):114-122.
[22]魂魏岚,陈亚华,钱猛,等.可降解螯合剂EDDS诱导植物修复重金属污染土壤的潜力[J].南京农业大学学报,2006,29(2):33-38.
[23]Evangelou MWH,Ebel M,Schaeffer A.Chelate assisted phytoextraction of heavy metals from:Effect,mechanism,toxicity and fate of chelating agents[J].Chemosphere,2007,68(6):989-1103.
[24]Tandy S,Bossart K,Mueller R,et al.Extraction of heavy metals from soils using biodegradable chelating agents[J].Environmental Science and Technology,2004,38(3):937-944.
[25]Zhao L,Li T,Yu H,et al.Effects of[S,S]-ethylenediaminedisuccinic acid and nitrilotriacetic acid on the efficiency of Pb phytostabilization by Athyrium wardii(Hook.)grown in Pb-contaminated soils[J].Journal of Environmental Economics&Management,2016,182(1):94-100.
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