不同处理方式对土壤酶活性的影响
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摘要
本文分别采用靛酚蓝比色法、3. 5-二硝基水杨酸法、高锰酸钾滴定的方法,探究在土壤中种植大豆、加入粘质沙雷氏菌和咪唑乙烟酸及三者相互作用对土壤脲酶、蔗糖酶、过氧化氢酶活性的影响。结果表明:在土壤中种植大豆后,土壤脲酶、蔗糖酶和过氧化氢酶活性在培养10～30 d中均呈现激活趋势;在土壤中加入粘质沙雷氏菌,5～20 d中,土壤脲酶、蔗糖酶活性均呈现激活趋势,粘质沙雷氏菌使土壤过氧化氢酶活性显著增加;在土壤中加入100 mg/kg浓度的咪唑乙烟酸,土壤脲酶呈现恢复—抑制的趋势,土壤蔗糖酶活性在10 d时开始激活,30 d时呈现抑制状态,过氧化氢酶活性在10 d和30 d时均有激活趋势;在土壤中种植大豆、加入粘质沙雷氏菌,再加入100 mg/kg的咪唑乙烟酸,土壤脲酶呈现抑制—恢复—抑制的趋势,土壤蔗糖酶活性呈现激活—恢复—抑制—激活的趋势,土壤过氧化氢酶活性呈现抑制—激活—抑制的趋势。综上所述,说明不同处理方式对土壤脲酶、蔗糖酶和过氧化氢酶活性产生的变化显著。
In order to explore the effects of adding soybean,serratia marcescens and imidazolidinic acid on the soil urease,sucrase and catalase activities,the indophenol blue colorimetric method and 3. 5-dinitrogen were used respectively. The results showed that the soil urease,sucrase and catalase activities showed activating trend in the culture for 10 ～ 30 d after planting soybean in the soil.Serratia marcescens was added to the soil,after 5 ～ 20 d,soil urease and sucrase activities showed activating trend,which meant that serratia marcescens significantly increased soil catalase activity. Adding 100 mg/kg imidazolidinic acid to soil,soil urease showed recovery-suppression trend,soil sucrose enzyme activity began to activate at 10 d,and inhibited at 30 d. Catalase activity was activated at 10 d and 30 d. Soy was planted in soil,added with serratia marcescens and 100 mg/kg imidazolium acid,soil urease showed a trend of inhibition-recovery-inhibition,the soil invertase activity showed a trend of activation-recovery-inhibition-activation,and soil catalase activity showed a trend of inhibition-activation-suppression. In summary,the changes in soil urease,sucrase and catalase activities were significantly different for different treatments.
In order to explore the effects of adding soybean,serratia marcescens and imidazolidinic acid on the soil urease,sucrase and catalase activities,the indophenol blue colorimetric method and 3. 5-dinitrogen were used respectively. The results showed that the soil urease,sucrase and catalase activities showed activating trend in the culture for 10 ～ 30 d after planting soybean in the soil.Serratia marcescens was added to the soil,after 5 ～ 20 d,soil urease and sucrase activities showed activating trend,which meant that serratia marcescens significantly increased soil catalase activity. Adding 100 mg/kg imidazolidinic acid to soil,soil urease showed recovery-suppression trend,soil sucrose enzyme activity began to activate at 10 d,and inhibited at 30 d. Catalase activity was activated at 10 d and 30 d. Soy was planted in soil,added with serratia marcescens and 100 mg/kg imidazolium acid,soil urease showed a trend of inhibition-recovery-inhibition,the soil invertase activity showed a trend of activation-recovery-inhibition-activation,and soil catalase activity showed a trend of inhibition-activation-suppression. In summary,the changes in soil urease,sucrase and catalase activities were significantly different for different treatments.
引文
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[19]姚健,王让会,赵建萍,等.精河沙区土壤酶分布特征及其对土壤理化性状的响应[J].湖北农业科学,2015,(18):4435-4440.YAO J,WANG R H,ZHAO J P,et al. The distribution charac-teristics of soil enzyme and its response to physicochemical proper-ties of soil in the dessert area of Jinghe[J]. Hubei AgriculturalScience,2015(18):4435-4440.
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[2]高彦波,翟鹏辉,谭德远.土壤酶活性与土壤肥力的关系研究进展[J].安徽农业科学,2015,43(6):100-101.GAO Y B,ZHAI P H,TAN D Y. Research progress of relationshipbetween soil enzyme activity and soil fertility[J]. Journal of AnhuiAgricultural Sciences,2015,43(6):100-101.
[3] BANERJEE S,BORA S,THRALL P H,et al. Soil C and N ascausal factors of spatial variation in extracellular enzyme activityacross grassland-woodland ecotones[J]. Applied Soil Ecology,2016,105:1-8.
[4]李军辉,夏清,杨珍平,等.基施氮肥形态及用量对小麦根际土壤脲酶活性垂直分布的影响[J].山西农业科学,2018,46(11):1852-1858.LI J H,XIA Q,YANG Z P,et al. Effects of different kinds of bas-al application slow-release fertilizers and dosages on vertical distri-bution of urease activity in wheat rhizosphere soil[J]. Journal ofShanxi Agricultural Sciences,2018,46(11):1852-1858.
[5]范昆,王开运,王东,等. 1,3-二氯丙烯对土壤脲酶和蔗糖酶活性的影响[J].农药学学报,2006,8(2):139-142.FAN K,WANG K Y,WANG D,et al. The effect of 1,3-dichlo-ropropene on activities of soil urease and invertase[J]. ChineseJournal of Pesticide Science,2006,8(2):139-142.
[6]曹慧,孙辉,杨浩,等.土壤酶活性及其对土壤质量的指示研究进展[J].应用与环境生物学报. 2003,9(1):105-109.CAO H,SUN H,YANG H,et al. A review soil enzyme activityand its indication for soil quality[J]. Chinese Journal of AppliedEnvironmental Biology,2003,9(1):105-109.
[7]王建,卢超超,芦珊珊,等.蔬菜大棚土壤过氧化氢酶活性及与养分关系研究[J].甘肃科学学报,2018,30(2):45-46.WANG J,LU C C,LU S S,et al. Study on the relationship be-tween soil catalase activity and nutrient in vegetable greenhouse[J]. Journal of Gansu Sciences,2018,30(2):45-46.
[8] ZIMMERMANN S,FREY B. Soil respiration and microbial proper-ties in an acid forest soil:effects of wood ash[J]. Soil Bio1ogy andBiochemistry,2002,34(11):1727-1737.
[9]黄永洪,花慧,等.猪肝过氧化氢酶提取条件的研究[J].生物技术通讯,2005,16(1):40-42.HUANG Y H,HUA H,et al. Study on extraction of catalase frompig liver[J]. Letters in Biotechnology,2005,16(1):40-42.
[10]刘善江,夏雪,陈桂梅,等.土壤酶的研究进展[J].中国农学通报,2011,27(21):1-7.LIU S J,XIA X,CHEN G M,et al. Study progress on functionsand affecting factors of soil enzymes[J]. Chinese Agricultural Sci-ence Bulletin,2011,27(21):1-7.
[11]陈少杰,邓恩.桉林地土壤酶活性与土壤养分关系研究[J].安徽农学通报,2009,15(15):171-172.CHEN S J,DENG E. Study on the relationship between soil en-zyme activity and soil nutrients of eucalyptus[J]. Auhui Agricul-tural Science Bulletin,2009,15(15):171-172.
[12]孙彩霞,陈利军,武志杰. Bt杀虫晶体蛋白的土壤残留及其对土壤磷酸酶活性的影响[J].土壤学报,2004,41(5):454-458.SUN C X,CHEN L J,WU Z J. Soil residuals of Bt insecticidalcrystal protein and their effects on soil phosphatase activity[J].Acta Pedologica Sinica,2004,41(5):454-458.
[13]毕淑芹,张丽娟,贺晓敏,等.坡地土壤酶流失规律初探[J].农业资源与环境科学,2006,22(7):500-503.BI S Q,ZHANG L J,HE X M,et al. Primary research on soilenzymes runoff rules from sloping field[J]. Chinese AgriculturalScience Bulletin,2006,22(7):500-503.
[14]郑银侠,马飞明,曹万虎,等.有机生物肥料对冬小麦生长及产量影响的研究[J].陕西农业科学,2006,52(1):22-23.ZHENG Y X,MA F M,CAO W H,et al. Effect of organic bio-fertilizer on growth and yield of winter wheat[J]. Shanxi Agricul-tural Sciences,2006,52(1):22-23.
[15]杨万勤,王开运.森林土壤酶的研究进展[J].林业科学,2004,40(2):152-159.YANG W Q,WANG K Y. Advances in forest soil enzymology[J]. Scientia Silvae Sinicae,2004,40(2):152-159.
[16]关松荫.土壤酶与土壤肥力[J].土壤通报,1980,11(6):40-41.GUAN S Y. Soil enzymes and soil fertility[J]. Chinese Journal ofSoil Science,1980,11(6):40-41.
[17]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.GUAN S Y. Soil enzymes and its methodology[M]. Beijing:Ag-ricultural Press,1986.
[18]周礼恺.土壤酶学[M].北京:科学出版社,1987.ZHOU L K. Soil enzymology[M]. Beijing:Science Press,1987.
[19]姚健,王让会,赵建萍,等.精河沙区土壤酶分布特征及其对土壤理化性状的响应[J].湖北农业科学,2015,(18):4435-4440.YAO J,WANG R H,ZHAO J P,et al. The distribution charac-teristics of soil enzyme and its response to physicochemical proper-ties of soil in the dessert area of Jinghe[J]. Hubei AgriculturalScience,2015(18):4435-4440.
[20]徐欣,王晓军,谢洪宝,等.秸秆腐解对不同氮肥水平土壤脲酶活性的影响[J].中国农学通报,2018,34(34):99-102.YAO X,XIE X J,XIE H B,et al. Effects of straw decompositionon soil urease activity at different nitrogen fertilizer levels[J].Chinese Agricultural Science Bulletin,2018,34(34):99-102.
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