甘蔗渣碳源释放规律及其硫酸盐还原菌利用性试验
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摘要
针对传统有机碳源使用费用高、易产生二次污染等问题,采用农业废弃物甘蔗渣作为碳源,开展甘蔗渣在不同pH、不同粒径、不同投加量条件下的单因素试验和在不同硫酸盐还原菌(SRB)投加量、不同甘蔗渣粒径及其不同投加量条件下的正交试验,研究碳源释放规律以及SRB利用甘蔗渣的最佳组合条件。结果表明,在pH值为7的体系、甘蔗渣粒径和投量分别为60目、3.5 g·100 mL~(-1)废水时,甘蔗渣中纤维素水解速率及葡萄糖水解速率均较慢,有利于还原糖的持续积累。在100 mL废水中,SRB投加量为71.2 mg、甘蔗渣粒径和投量分别为100目和4.5 g时,SO_4~(2-)去除效果最好、还原糖水解速率最小,为正交试验的最佳组合。在此组合条件下,甘蔗渣持续供给碳源96 h后,反应体系中SO_4~(2-)去除率和还原糖释放量仍分别高达75.23%、64.03 mg·g~(-1),氧化还原电位(ORP)低至-224 mV。
Aiming at the problems of high cost and easy release of secondary pollution during the use of traditional organic carbon sources,single factor experiments and orthogonal experiments were performed to study the release law of the carbon source and the optimal condition of sulfate-reducing bacteria(SRB)using bagasse. The single factor experiments were carried out at different pH values, with different bagasse particle sizes, and with different bagasse dosages. The orthogonal experiments were carried out with different SRB dosages, different bagasse particle sizes, and different bagasse dosages. The results of the single factor experiments showed that the hydrolysis rates of cellulose and glucose in bagasse were slower when the wastewater pH value was 7 and the particle size and dosage of bagasse were 60 mesh and 3.5 g·100 mL~(-1), respectively, which would be beneficial to the continuous accumulation of reducing sugar. The results of the orthogonal experiments showed that the removal effect of SO_4~(2-) was the best and the hydrolysis rate of reducing sugar was the lowest when the SRB dosage was 71.2 mg·100 mL~(-1) and the particle size and dosage of bagasse were 100 mesh and 4.5 g·100 mL~(-1), respectively, which was determined as the best combination of orthogonal experimental conditions. Under the best combination conditions, the removal rate of SO_4~(2-) and release amount of reducing sugar in the solution were still 75.23% and 64.03 mg·g~(-1), respectively, and the oxidation-reduction potential(ORP)was -224 mV, after the bagasse was continuously supplied as a carbon source for 96 hours.
Aiming at the problems of high cost and easy release of secondary pollution during the use of traditional organic carbon sources,single factor experiments and orthogonal experiments were performed to study the release law of the carbon source and the optimal condition of sulfate-reducing bacteria(SRB)using bagasse. The single factor experiments were carried out at different pH values, with different bagasse particle sizes, and with different bagasse dosages. The orthogonal experiments were carried out with different SRB dosages, different bagasse particle sizes, and different bagasse dosages. The results of the single factor experiments showed that the hydrolysis rates of cellulose and glucose in bagasse were slower when the wastewater pH value was 7 and the particle size and dosage of bagasse were 60 mesh and 3.5 g·100 mL~(-1), respectively, which would be beneficial to the continuous accumulation of reducing sugar. The results of the orthogonal experiments showed that the removal effect of SO_4~(2-) was the best and the hydrolysis rate of reducing sugar was the lowest when the SRB dosage was 71.2 mg·100 mL~(-1) and the particle size and dosage of bagasse were 100 mesh and 4.5 g·100 mL~(-1), respectively, which was determined as the best combination of orthogonal experimental conditions. Under the best combination conditions, the removal rate of SO_4~(2-) and release amount of reducing sugar in the solution were still 75.23% and 64.03 mg·g~(-1), respectively, and the oxidation-reduction potential(ORP)was -224 mV, after the bagasse was continuously supplied as a carbon source for 96 hours.
引文
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[14]闫加贺.生物质在H2O-SO2体系中两步法水解转化的研究[D].北京:北京化工大学,2014.YAN Jia-he.Study on two-step hydrolysis of biomass in H2O-SO2system[D].Beijing:Beijing University of Chemical Technology,2014.
[15]杨茜.可渗透固定化硫酸盐还原菌反应墙的碳源缓释规律与可利用性研究[D].芜湖:安徽工程大学,2014.YANG Qian.Study on the slow release law and availability of carbon source in permeable sulfate-reducing bacteria reaction wall[D].Wuhu:Anhui Polytechnic University,2014.
[16]姜楠,谢楠,齐崴,等.硫酸催化葡萄糖制备乙酰丙酸的过程强化[J].化工进展,2014,33(11):2888-2893.JIANG Nan,XIE Nan,QI Wei,et al.Process Strengthening of glucose catalyzed by glucose to prepare levulinic acid[J].Chemical Industry and Engineering Progress,2014,33(11):2888-2893.
[17]Kumar S,Gupta R,Lee Y Y,et al.Cellulose pretreatment in subcritical water:Effect of temperature on molecular structure and enzymatic reactivity[J].Bioresource Technol,2010,101(4):1337-1347.
[18]邓奥杰.玉米芯两步法高效制备糠醛的研究[D].广州:华南理工大学,2016.DENG Ao-jie.Study on the efficient preparation of furfural by corn cob two-step method[D].Guangzhou:South China University of Technology,2016.
[19]曹雅俊,张广积,毛在砂.硫酸盐还原菌对不同碳源的利用率[J].中国有色金属学报,2008,18(1):96-100.CAO Ya-jun,ZHANG Guang-ji,MAO Zai-sha.The utilization ratio of sulfate reducing bacteria to different carbon sources[J].The Chinese Journal of Nonferrous Metals,2008,18(1):96-100.1157
[2]李洪静,陈银广,顾国维.丙酸/乙酸对低能耗生物除磷脱氮系统的影响[J].中国环境科学,2008(8):673-678.LI Hong-jing,CHEN Yin-guang,GU Guo-wei.Effect of propionic acid/acetic acid on low energy consumption biological phosphorus and nitrogen removal system[J].China Environmental Science,2008(8):673-678.
[3]邵留,徐祖信,尹海龙.污染水体脱氮工艺中外加碳源的研究进展[J].工业水处理,2007(12):10-14.SHAO Liu,XU Zu-xin,YIN Hai-long.Research progress on carbon source in denitrification process of polluted water bodies[J].Industrial Water Treatment,2007(12):10-14.
[4]赵联芳,朱伟,高青.补充植物碳源提高人工湿地脱氮效率[J].解放军理工大学学报(自然科学版),2009,10(6):644-649.ZHAO Lian-fang,ZHU Wei,GAO Qing.Supplementing plant carbon source to improve nitrogen removal efficiency of constructed wetland[J].Journal of PLA University of Science and Technology(Natural Science Edition),2009,10(6):644-649.
[5]Ovez B.Batch biological denitrification using Arundo donax,Glycyrrhiza glabra,and Gracilaria verrucosa as carbon source[J].Process Biochemistry,2005,41:1289-1295.
[6]Ovez B,Ozgen S,Yuksel M.Biological denitrification in drinking water using Glycyrrhiza glabra and Arundo donax as the carbon source[J].Process Biochemistry,2006,41:1539-1544.
[7]邵留,徐祖信,金伟,等.以稻草为碳源和生物膜载体去除水中的硝酸盐[J].环境科学,2009,30(5):1414-1419.SHAO Liu,XU Zu-xin,JIN Wei,et al.Removal of nitrate from water by using rice straw as carbon source and biofilm carrier[J].Environmental Science,2009,30(5):1414-1419.
[8]李晔,章旻,陈家宏,等.污水反硝化脱氮的固态有机碳源选择实验研究[J].武汉理工大学学报,2010,32(6):27-31.LI Ye,ZHANG Min,CHEN Jia-hong,et al.Experimental study on selection of solid organic carbon source for denitrification and denitrification of wastewater[J].Journal of Wuhan University of Technology,2010,32(6):27-31.
[9]曹文平,张永明,李亚峰,等.竹丝填充床对高有机负荷及低C/N水质的脱氮特性[J].中国环境科学,2010,30(8):1067-1072.CAO Wen-ping,ZHANG Yong-ming,LI Ya-feng,et al.Denitrification characteristics of bamboo filled bed for high organic load and low C/N water quality[J].China Environmental Science,2010,30(8):1067-1072.
[10]张雅琳,胡学伟,夏丽娟,等.甘蔗渣为缓释碳源负载SRB处理模拟矿山淋滤水[J].环境工程学报,2016,10(5):2355-2360.ZHANG Ya-lin,HU Xue-wei,XIA Li-juan,et al.Sugarcane bagasse for slow release carbon source loading SRB treatment simulated mine leaching water[J].Journal of Environmental Engineering,2016,10(5):2355-2360.
[11]邵留,徐祖信,金伟,等.农业废物反硝化固体碳源的优选[J].中国环境科学,2011,31(5):748-754.SHAO Liu,XU Zu-xin,JIN Wei,et al.Optimization of denitrifying solid carbon source for agricultural waste[J].China Environmental Science,2011,31(5):748-754.
[12]张雯,张亚平,尹琳,等.以10种农业废弃物为基料的地下水反硝化碳源属性的实验研究[J].环境科学学报,2017,37(5):1787-1797.ZHANG Wen,ZHANG Ya-ping,YIN Lin,et al.Experimental study on the properties of denitrifying carbon source in groundwater based on 10 kinds of agricultural wastes[J].Acta Scientiae Circumstantiae,2017,37(5):1787-1797.
[13]赵莹.硫酸盐还原菌的分离及金属离子去除机理的研究[D].吉林:吉林大学,2016.ZHAO Ying.Separation of sulfate-reducing bacteria and mechanism of metal ion removal[D].Jilin:Jilin University,2016.
[14]闫加贺.生物质在H2O-SO2体系中两步法水解转化的研究[D].北京:北京化工大学,2014.YAN Jia-he.Study on two-step hydrolysis of biomass in H2O-SO2system[D].Beijing:Beijing University of Chemical Technology,2014.
[15]杨茜.可渗透固定化硫酸盐还原菌反应墙的碳源缓释规律与可利用性研究[D].芜湖:安徽工程大学,2014.YANG Qian.Study on the slow release law and availability of carbon source in permeable sulfate-reducing bacteria reaction wall[D].Wuhu:Anhui Polytechnic University,2014.
[16]姜楠,谢楠,齐崴,等.硫酸催化葡萄糖制备乙酰丙酸的过程强化[J].化工进展,2014,33(11):2888-2893.JIANG Nan,XIE Nan,QI Wei,et al.Process Strengthening of glucose catalyzed by glucose to prepare levulinic acid[J].Chemical Industry and Engineering Progress,2014,33(11):2888-2893.
[17]Kumar S,Gupta R,Lee Y Y,et al.Cellulose pretreatment in subcritical water:Effect of temperature on molecular structure and enzymatic reactivity[J].Bioresource Technol,2010,101(4):1337-1347.
[18]邓奥杰.玉米芯两步法高效制备糠醛的研究[D].广州:华南理工大学,2016.DENG Ao-jie.Study on the efficient preparation of furfural by corn cob two-step method[D].Guangzhou:South China University of Technology,2016.
[19]曹雅俊,张广积,毛在砂.硫酸盐还原菌对不同碳源的利用率[J].中国有色金属学报,2008,18(1):96-100.CAO Ya-jun,ZHANG Guang-ji,MAO Zai-sha.The utilization ratio of sulfate reducing bacteria to different carbon sources[J].The Chinese Journal of Nonferrous Metals,2008,18(1):96-100.1157
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