深圳杨梅坑海域造礁石珊瑚共生藻分类和遗传多样性研究
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摘要
通过核糖体ITS(Internal Transcribed Spacer)的核苷酸序列研究了深圳杨梅坑海域20种47个造礁石珊瑚样本的共生藻。通过ITS序列分析,与GenBank上的4种不同的共生藻构建Neighbor-Joining聚类树,进行石珊瑚共生藻分类和遗传多样性分析。结果表明,该海域的造礁石珊瑚共生藻属于2种不同的种类(亚系群),19个样品属于C1亚系群共生藻,1个样品属于C15亚系群共生藻,C1和C15两个亚系群共生藻之间的遗传距离为0. 01。将深圳杨梅坑海域得到的ITS序列与NCBI数据库上的福建东山海域、广东徐闻地区的C1系共生藻进行比对,只有深圳杨梅坑海域藻类样本平均(A+T)碱基含量为49. 4%,(A+T)含量小于(G+C)含量。构建Neighbor-Joining聚类树表明,深圳杨梅坑海域石珊瑚共生藻与福建东山海域的亲缘关系较近,而与广东徐闻地区的亲缘关系较远,地理隔离是主要的因素。
This study analyzed the nucleotide sequences of Internal Transcribed Spacer( ITS) from Symbiodinium of 20 coral species. A total of 47 samples were collected from Yangmeikeng sea area in Shenzhen. Through ITS sequence analysis,a Neighbor-Joining tree was constructed with 4 different Symbiodinium groups in GenBank. The results show that Symbiodinium of scleractinia corals in this sea area belong to 2 different species( subgroups),19 samples belong to the C1 subgroup,1 sample belongs to the C15 subgroup. The Symbiodinium's genetic distance between C1 and C2 subpopulations is 0. 01. Comparing with the Symbiodinium's ITS sequences of C1 subgroup obtained from Yangmeikeng sea area in Shenzhen,the Dongshan sea area of Fujian Province and the Xuwen sea area of Guangdong Province,we found that the average( A + T) base content of algal samples is 49. 4%,which is less than( G + C) in Yangmeikeng sea area. The construction of Neighbor-Joining tree shows that Symbiodiniu from Dongshan and Yangmeikeng sea areas has a closer relationship compared with that from Xuwen. Thus,it is suggested that geographical isolation may be the main factor for the difference.
This study analyzed the nucleotide sequences of Internal Transcribed Spacer( ITS) from Symbiodinium of 20 coral species. A total of 47 samples were collected from Yangmeikeng sea area in Shenzhen. Through ITS sequence analysis,a Neighbor-Joining tree was constructed with 4 different Symbiodinium groups in GenBank. The results show that Symbiodinium of scleractinia corals in this sea area belong to 2 different species( subgroups),19 samples belong to the C1 subgroup,1 sample belongs to the C15 subgroup. The Symbiodinium's genetic distance between C1 and C2 subpopulations is 0. 01. Comparing with the Symbiodinium's ITS sequences of C1 subgroup obtained from Yangmeikeng sea area in Shenzhen,the Dongshan sea area of Fujian Province and the Xuwen sea area of Guangdong Province,we found that the average( A + T) base content of algal samples is 49. 4%,which is less than( G + C) in Yangmeikeng sea area. The construction of Neighbor-Joining tree shows that Symbiodiniu from Dongshan and Yangmeikeng sea areas has a closer relationship compared with that from Xuwen. Thus,it is suggested that geographical isolation may be the main factor for the difference.
引文
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[2]周国伟,黄晖,喻子牛,等.造礁石珊瑚与其共生藻(Symbiodinium)共生研究进展[J].生态学报,2009,29(8):4 397-4 407.
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[8]黄晖,尤丰,练健生,等.西沙群岛海域造礁石珊瑚物种多样性与分布特点[J].生物多样性,2011,19(6):710-715.
[9]李元超,杨毅,郑新庆,等.海南三亚后海海域珊瑚礁生态系统的健康状况及其影响因素[J].生态学杂志,2015,34(4):1 105-1 112.
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[12] DAI C F,HORNG S. Scleractinia fauna of Taiwan I. The complex group[M]. Taipei:National Taiwan University,2009.
[13]谢子强,廖宝林,肖宝华,等.运用ITS基因分析大鹏半岛海域石珊瑚系统发育关系[J].广东海洋大学学报,2017,37(4):8-15.
[14]董志军,黄晖,黄良民,等.运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成[J].生物多样性,2008,16(5):498-502.
[15]姜自锋,窦向梅,黄大卫.系统发育研究中多重序列比对常见问题分析[J].动物学报分类,2006,31(1):81-87.
[16]卫滢.巴戟天及其6种近缘植物的鉴别研究[D].广州:广州中医药大学,2017.
[17]张丽娜,荣昌鹤,何远,等.常用系统发育树构建算法和软件鸟瞰[J].动物学研究,2013,34(6):640-650.
[18]董志军,黄晖,黄良民,等.福建东山岛附近海域造礁石珊瑚共生藻的分子系统分类和遗传多样性研究[J]台湾海峡,2008,27(2):135-140.
[19]刘丽,陈育盛,申玉春,等.造礁石珊瑚共生藻的分子分类研究[J].海洋与湖沼,2012,43(4):718-722.
[20]程希婷.石珊瑚的DNA条形码研究及其系统发育分析[D].海口:海南大学,2012.
[21] HUANG H,DONG Z J,HUANG L M,et al. Restriction fragment length polymorphism analysis of large subunit r DNA of symbiotic dinoflagellates from scleractinian corals in the Zhubi Coral Reef of the Nansha Islands[J]. Journal of Integrative Plant Biology,2006,48(2):148-152.
[22] BAKER A C,STARGER C J,MCCLANAHAN T R,et al. Corals'adaptive response to climate change[J]. Nature,2004,430(7 001):741.
[23] LAJEUNESSE T C,LOH W K W,WOESIK R V,et al. Low symbiont diversity in southern Great Barrier Reef corals,relative to those of the Caribbean[J]. Limnology&Oceanography,2003,48(5):2 046-2 054.
[24]许婷,曹永港.南海海洋环流季节变化的数值模拟研究[J].海岸工程,2017,36(1):62-71.
[25]陈达森,陈波,严金辉,等.琼州海峡余流场季节性变化特征[J].海洋湖沼通报,2006(2):12-17.
[26]俎婷婷.北部湾环流及其机制的分析[D].青岛:中国海洋大学,2005.
[27]刘洋.南海次表层、中层水团结构及其运动学特征的研究[D].青岛:中国海洋大学,2010.
[28]贾春斌,唐振朝,周凯,等.深圳珊瑚图集[M].深圳:深圳报业集团出版社,2015.
[29] MARKELL D A. Macromolecules exuded by dinoflagellates in symbiosis:A biochemical and cellular analysis of specificity[D]. San Diego:University of California San Diego,1995.
[30]陈育盛.徐闻造礁石珊瑚及其共生藻分子系统学研究[D].广州:广东海洋大学,2012.
[2]周国伟,黄晖,喻子牛,等.造礁石珊瑚与其共生藻(Symbiodinium)共生研究进展[J].生态学报,2009,29(8):4 397-4 407.
[3]董志军,黄晖,黄良民,等.虫黄藻的分类和遗传多样性研究进展[J].海洋通报,2008,27(3):95-101.
[4]朱瑾.海洋中的热带雨林:珊瑚礁[J].海洋世界,2008(11):30-37.
[5]赵美霞,余克服,张乔民.珊瑚礁区的生物多样性及其生态功能[J].生态学报,2006,26(1):186-194.
[6] CHEN C,YANG Y N,TSAI W,et al. Symbiont diversity in scleractinian corals from tropical reefs and subtropical non-reef communities in Taiwan[J]. Coral Reefs,2005,24(1):11-22.
[7] KINZIE R A,TAKAYAMA M,SANTOS S R,et al. The adaptive bleaching hypothesis:experimental tests of critical assumptions[J]. Biological Bulletin,2001,200(1):51-58.
[8]黄晖,尤丰,练健生,等.西沙群岛海域造礁石珊瑚物种多样性与分布特点[J].生物多样性,2011,19(6):710-715.
[9]李元超,杨毅,郑新庆,等.海南三亚后海海域珊瑚礁生态系统的健康状况及其影响因素[J].生态学杂志,2015,34(4):1 105-1 112.
[10]邹仁林.中国动物志:腔肠动物门[M].北京:科学出版杜,2001.
[11] VERON J E N,STAFFORD S M. Corals of the world[M]. Townsville:Australian Institute of Marine Science,2000.
[12] DAI C F,HORNG S. Scleractinia fauna of Taiwan I. The complex group[M]. Taipei:National Taiwan University,2009.
[13]谢子强,廖宝林,肖宝华,等.运用ITS基因分析大鹏半岛海域石珊瑚系统发育关系[J].广东海洋大学学报,2017,37(4):8-15.
[14]董志军,黄晖,黄良民,等.运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成[J].生物多样性,2008,16(5):498-502.
[15]姜自锋,窦向梅,黄大卫.系统发育研究中多重序列比对常见问题分析[J].动物学报分类,2006,31(1):81-87.
[16]卫滢.巴戟天及其6种近缘植物的鉴别研究[D].广州:广州中医药大学,2017.
[17]张丽娜,荣昌鹤,何远,等.常用系统发育树构建算法和软件鸟瞰[J].动物学研究,2013,34(6):640-650.
[18]董志军,黄晖,黄良民,等.福建东山岛附近海域造礁石珊瑚共生藻的分子系统分类和遗传多样性研究[J]台湾海峡,2008,27(2):135-140.
[19]刘丽,陈育盛,申玉春,等.造礁石珊瑚共生藻的分子分类研究[J].海洋与湖沼,2012,43(4):718-722.
[20]程希婷.石珊瑚的DNA条形码研究及其系统发育分析[D].海口:海南大学,2012.
[21] HUANG H,DONG Z J,HUANG L M,et al. Restriction fragment length polymorphism analysis of large subunit r DNA of symbiotic dinoflagellates from scleractinian corals in the Zhubi Coral Reef of the Nansha Islands[J]. Journal of Integrative Plant Biology,2006,48(2):148-152.
[22] BAKER A C,STARGER C J,MCCLANAHAN T R,et al. Corals'adaptive response to climate change[J]. Nature,2004,430(7 001):741.
[23] LAJEUNESSE T C,LOH W K W,WOESIK R V,et al. Low symbiont diversity in southern Great Barrier Reef corals,relative to those of the Caribbean[J]. Limnology&Oceanography,2003,48(5):2 046-2 054.
[24]许婷,曹永港.南海海洋环流季节变化的数值模拟研究[J].海岸工程,2017,36(1):62-71.
[25]陈达森,陈波,严金辉,等.琼州海峡余流场季节性变化特征[J].海洋湖沼通报,2006(2):12-17.
[26]俎婷婷.北部湾环流及其机制的分析[D].青岛:中国海洋大学,2005.
[27]刘洋.南海次表层、中层水团结构及其运动学特征的研究[D].青岛:中国海洋大学,2010.
[28]贾春斌,唐振朝,周凯,等.深圳珊瑚图集[M].深圳:深圳报业集团出版社,2015.
[29] MARKELL D A. Macromolecules exuded by dinoflagellates in symbiosis:A biochemical and cellular analysis of specificity[D]. San Diego:University of California San Diego,1995.
[30]陈育盛.徐闻造礁石珊瑚及其共生藻分子系统学研究[D].广州:广东海洋大学,2012.
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