嫁接诱导植物镉富集性状的改变
|
摘要
以野生茄(Solanum torvum,托鲁巴姆)为砧木,茄(Solanum melongena)和番茄(Solanum lycopersicum)为接穗植物,通过田间小区实验,研究了嫁接对重金属Cd在接穗植物地上部分积累的影响,同时探究了元素S对植物Cd积累过程的影响。结果表明:与自根植株相比,对照小区与2 mg·kg-1Cd处理小区内,嫁接茄植株的茎、叶及果实Cd含量分别减少78.0%、86.3%、93.2%和89.1%、89.5%、92.3%;嫁接番茄植株的茎、叶及果实Cd含量分别减少20.6%、15.5%、10%和79.3%、48.3%、4.2%;同时,嫁接植株(茄、番茄)叶片中的总硫(TS)含量显著减少,其变化趋势与Cd积累变化趋势呈正相关关系。研究证明,特异性砧木可以改变嫁接植株重金属Cd富集性状,不同作物对同一砧木的响应存在种间差异,这种改变与嫁接植株S含量变化密切相关。
Using wild-type eggplant( Solanum torvum) as rootstock,a field plot experiment was conducted to examine the effects of grafting on Cd accumulation in the shoots of scion plants eggplant( Solanum melongena) and tomato( Solanum lycopersicum). We also explored the role of sulfur in driving plant Cd accumulation. Compared to self-rooted plants,Cd contents in stems,leaves,and fruits of grafted eggplants were reduced by 78. 0%,86. 3%,93. 2% in CK,and89.1%,89.5%,92. 3% in 2 mg ·kg-1Cd-polluted field plots,respectively. The decreases in grafted tomato plants were 20.6%,15.5%,10% in CK,and 79.3%,48.3%,4.2% in 2 mg·kg-1Cd-polluted field plots,respectively. Moreover,total sulfur contents in leaves of grafted plants( eggplant and tomato) were significantly reduced,and the trends of which were consistent with Cd accumulation. Our results indicated that specific rootstock induced the changes of Cd accumulation in grafted plants. There was interspecific difference in the responses of plants to the same rootstock. In addition,such changes of Cd accumulation in plants were closely related with the changes of total sulfur content.
Using wild-type eggplant( Solanum torvum) as rootstock,a field plot experiment was conducted to examine the effects of grafting on Cd accumulation in the shoots of scion plants eggplant( Solanum melongena) and tomato( Solanum lycopersicum). We also explored the role of sulfur in driving plant Cd accumulation. Compared to self-rooted plants,Cd contents in stems,leaves,and fruits of grafted eggplants were reduced by 78. 0%,86. 3%,93. 2% in CK,and89.1%,89.5%,92. 3% in 2 mg ·kg-1Cd-polluted field plots,respectively. The decreases in grafted tomato plants were 20.6%,15.5%,10% in CK,and 79.3%,48.3%,4.2% in 2 mg·kg-1Cd-polluted field plots,respectively. Moreover,total sulfur contents in leaves of grafted plants( eggplant and tomato) were significantly reduced,and the trends of which were consistent with Cd accumulation. Our results indicated that specific rootstock induced the changes of Cd accumulation in grafted plants. There was interspecific difference in the responses of plants to the same rootstock. In addition,such changes of Cd accumulation in plants were closely related with the changes of total sulfur content.
引文
董萌,赵运林,周小梅,等.2012.土壤镉污染现状与重金属修复方法研究.绿色科技,(4):212-215.
高宇,程潜,张梦君,等.2017.镉污染土壤修复技术研究.生物技术通报,33(10):103-110.
何海洋,彭方仁,张瑞,等.2016.嫁接繁殖研究进展及其在林木遗传改良中的应用前景.世界林业研究,29(4):25-29.
焦洪静,牛明芬,魏树和,等.2013.茄子苗对镉积累和耐性的品种间差异.生态毒理学报,8(3):413-418.
刘用生.2001.中国古今植物远缘嫁接的理论和实践意义.自然科学史研究,(4):352-361.
庞荣丽,王瑞萍,谢汉忠,等.2016.农业土壤中镉污染现状及污染途径分析.天津农业科学,22(12):87-91.
秦秦,李雪梅,台培东,等.2013.不同类型茄对镉的富集及嫁接对镉富集特性的影响.生态学杂志,32(8):2043-2048.
王燕,谢辉,陈利萍.2011.植物嫁接诱导的遗传变异机理的研究进展.遗传,33(6):585-590.
王一喆,王强.2008.镉对植物根系的毒害作用.广东微量元素科学,15(4):1-5.
吴海燕,台培东,李培军,等.2011.馒头柳对镉的耐性、运输途径和累积特征.生态学杂志,30(6):1222-1228.
姚曜晖.2017.国内重金属土壤修复技术应用现状.科技创新导报,14(19):105-106.
张永志,赵首萍,徐明飞,等.2009.不同蒸腾作用对番茄幼苗吸收Pb、Cd的影响.生态环境学报,18(2):515-518.
赵其国,沈仁芳,滕应,等.2017.中国重金属污染区耕地轮作休耕制度试点进展、问题及对策建议.生态环境学报,26(12):2003-2007.
Arao T,Takeda H,Nishihara E.2008.Reduction of cadmium translocation from roots to shoots in eggplant(Solanum melongena)by grafting onto Solanum torvum rootstock.Soil Science&Plant Nutrition,54:555-559.
Cakmak I,Welch R M,Hart J,et al.2000.Uptake and retranslocation of leaf-applied cadmium(109Cd)in diploid,tetraploid and hexaploid wheats.Journal of Experimental Botany,51:221-226.
Chen A,Komives EA,Schroeder JI.2006.An improved grafting technique for mature Arabidopsis plants demonstrates longdistance shoot-to-root transport of phytochelatins in Arabidopsis.Plant Physiology,141:108-120.
Gong JM,Lee DA,Schroeder JI.2003.Long-distance root-toshoot transport of phytochelatins and cadmium in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,100:10118-10123.
Guimar2es MdA,Gustin JL,Salt DE.2009.Reciprocal grafting separates the roles of the root and shoot in zinc hyperaccumulation in Thlaspi caerulescens.New Phytologist,184:323-329.
Liang J,Shohag MJI,Yang X,et al.2014.Role of sulfur assimilation pathway in cadmium hyperaccumulation by Sedum alfredii Hance.Ecotoxicology and Environmental Safety,100:159-165.
Liu YS,Wang QL,Li BY.2010.New insights into plant graft hybridization.Heredity,104:1-2.
Mendoza-Cozatl D,Butko E,Springer F,et al.2008.Identification of high levels of phytochelatins,glutathione and cadmium in the phloem sap of Brassica napus.A role for thiolpeptides in the long-distance transport of cadmium and the effect of cadmium on iron translocation.Plant Journal,54:249-259.
Mori S,Uraguchi S,Ishikawa S,et al.2009.Xylem loading process is a critical factor in determining Cd accumulation in the shoots of Solanum melongena and Solanum torvum.Environmental and Experimental Botany,67:127-132.
Rouphael Y,Cardarelli M,Rea E,et al.2008.Grafting of cucumber as a means to minimize copper toxicity.Environmental and Experimental Botany,63:49-58.
Savvas D,Colla G,Rouphael Y,et al.2010.Amelioration of heavy metal and nutrient stress in fruit vegetables by grafting.Scientia Horticulturae,127:156-161.
Sillen LG.1964.Stability Constants of Metal-ion Complexes.Section 1:Inorganic Ligands.London:The Chemical Society Burlington House.
Stegemann S,Bock R.2009.Exchange of genetic material between cells in plant tissue grafts.Science,324:649-651.
Xin J.2013.Role of roots in cadmium accumulation of two water spinach cultivars:Reciprocal grafting and histochemical experiments.Plant and Soil,366:425-432.
Xu J,Zhu Y,Ge Q,et al.2012.Comparative physiological responses of Solanum nigrum and Solanum torvum to cadmium stress.New Phytologist,196:125-138.
Yamaguchi N,Mori S,Baba K,et al.2011.Cadmium distribution in the root tissues of solanaceous plants with contrasting root-to-shoot Cd translocation efficiencies.Environmental and Experimental Botany,71:198-206.
Zhang W,Lin K,Zhou J,et al.2013.Spatial distribution and toxicity of cadmium in the joint presence of sulfur in rice seedling.Environmental Toxicology and Pharmacology,36:1235-1241.
Zhang Y,Zhao S,Xu M,et al.2009.Effect of transpiration rates on the absorption of Pb and Cd in seedling of tomato.Ecology and Environmental Sciences,18:515-518.
Zhao X,Guo Y,Huber DJ,et al.2011.Grafting effects on postharvest ripening and quality of 1-methylcyclopropenetreated muskmelon fruit.Scientia Horticulturae,130:581-587.
高宇,程潜,张梦君,等.2017.镉污染土壤修复技术研究.生物技术通报,33(10):103-110.
何海洋,彭方仁,张瑞,等.2016.嫁接繁殖研究进展及其在林木遗传改良中的应用前景.世界林业研究,29(4):25-29.
焦洪静,牛明芬,魏树和,等.2013.茄子苗对镉积累和耐性的品种间差异.生态毒理学报,8(3):413-418.
刘用生.2001.中国古今植物远缘嫁接的理论和实践意义.自然科学史研究,(4):352-361.
庞荣丽,王瑞萍,谢汉忠,等.2016.农业土壤中镉污染现状及污染途径分析.天津农业科学,22(12):87-91.
秦秦,李雪梅,台培东,等.2013.不同类型茄对镉的富集及嫁接对镉富集特性的影响.生态学杂志,32(8):2043-2048.
王燕,谢辉,陈利萍.2011.植物嫁接诱导的遗传变异机理的研究进展.遗传,33(6):585-590.
王一喆,王强.2008.镉对植物根系的毒害作用.广东微量元素科学,15(4):1-5.
吴海燕,台培东,李培军,等.2011.馒头柳对镉的耐性、运输途径和累积特征.生态学杂志,30(6):1222-1228.
姚曜晖.2017.国内重金属土壤修复技术应用现状.科技创新导报,14(19):105-106.
张永志,赵首萍,徐明飞,等.2009.不同蒸腾作用对番茄幼苗吸收Pb、Cd的影响.生态环境学报,18(2):515-518.
赵其国,沈仁芳,滕应,等.2017.中国重金属污染区耕地轮作休耕制度试点进展、问题及对策建议.生态环境学报,26(12):2003-2007.
Arao T,Takeda H,Nishihara E.2008.Reduction of cadmium translocation from roots to shoots in eggplant(Solanum melongena)by grafting onto Solanum torvum rootstock.Soil Science&Plant Nutrition,54:555-559.
Cakmak I,Welch R M,Hart J,et al.2000.Uptake and retranslocation of leaf-applied cadmium(109Cd)in diploid,tetraploid and hexaploid wheats.Journal of Experimental Botany,51:221-226.
Chen A,Komives EA,Schroeder JI.2006.An improved grafting technique for mature Arabidopsis plants demonstrates longdistance shoot-to-root transport of phytochelatins in Arabidopsis.Plant Physiology,141:108-120.
Gong JM,Lee DA,Schroeder JI.2003.Long-distance root-toshoot transport of phytochelatins and cadmium in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,100:10118-10123.
Guimar2es MdA,Gustin JL,Salt DE.2009.Reciprocal grafting separates the roles of the root and shoot in zinc hyperaccumulation in Thlaspi caerulescens.New Phytologist,184:323-329.
Liang J,Shohag MJI,Yang X,et al.2014.Role of sulfur assimilation pathway in cadmium hyperaccumulation by Sedum alfredii Hance.Ecotoxicology and Environmental Safety,100:159-165.
Liu YS,Wang QL,Li BY.2010.New insights into plant graft hybridization.Heredity,104:1-2.
Mendoza-Cozatl D,Butko E,Springer F,et al.2008.Identification of high levels of phytochelatins,glutathione and cadmium in the phloem sap of Brassica napus.A role for thiolpeptides in the long-distance transport of cadmium and the effect of cadmium on iron translocation.Plant Journal,54:249-259.
Mori S,Uraguchi S,Ishikawa S,et al.2009.Xylem loading process is a critical factor in determining Cd accumulation in the shoots of Solanum melongena and Solanum torvum.Environmental and Experimental Botany,67:127-132.
Rouphael Y,Cardarelli M,Rea E,et al.2008.Grafting of cucumber as a means to minimize copper toxicity.Environmental and Experimental Botany,63:49-58.
Savvas D,Colla G,Rouphael Y,et al.2010.Amelioration of heavy metal and nutrient stress in fruit vegetables by grafting.Scientia Horticulturae,127:156-161.
Sillen LG.1964.Stability Constants of Metal-ion Complexes.Section 1:Inorganic Ligands.London:The Chemical Society Burlington House.
Stegemann S,Bock R.2009.Exchange of genetic material between cells in plant tissue grafts.Science,324:649-651.
Xin J.2013.Role of roots in cadmium accumulation of two water spinach cultivars:Reciprocal grafting and histochemical experiments.Plant and Soil,366:425-432.
Xu J,Zhu Y,Ge Q,et al.2012.Comparative physiological responses of Solanum nigrum and Solanum torvum to cadmium stress.New Phytologist,196:125-138.
Yamaguchi N,Mori S,Baba K,et al.2011.Cadmium distribution in the root tissues of solanaceous plants with contrasting root-to-shoot Cd translocation efficiencies.Environmental and Experimental Botany,71:198-206.
Zhang W,Lin K,Zhou J,et al.2013.Spatial distribution and toxicity of cadmium in the joint presence of sulfur in rice seedling.Environmental Toxicology and Pharmacology,36:1235-1241.
Zhang Y,Zhao S,Xu M,et al.2009.Effect of transpiration rates on the absorption of Pb and Cd in seedling of tomato.Ecology and Environmental Sciences,18:515-518.
Zhao X,Guo Y,Huber DJ,et al.2011.Grafting effects on postharvest ripening and quality of 1-methylcyclopropenetreated muskmelon fruit.Scientia Horticulturae,130:581-587.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |