等离子体处理对不同含水量穿心莲种子萌发的影响
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摘要
为探究大气压等离子体处理对不同含水量穿心莲(Andrographis paniculata)种子萌发的影响,通过调节穿心莲种子含水量分别为6.3%、8.6%、12.9%、18.6%、24.0%和33.4%,再进行等离子体处理,经过发芽试验,以发芽势、发芽率、发芽指数、胚根长、活力指数、霉变率、正常幼苗百分比等指标,比较不同含水量的种子受到等离子体处理后的萌发效果。结果表明,穿心莲种子的含水量在12.9%、18.6%、24.0%时,经过大气等离子体处理后种子发芽率、发芽指数得到显著提高;同时种子和幼苗霉变坏死率显著下降,正常幼苗百分比提高。然而,对含水量为6.3%、8.6%和33.4%的种子进行处理,达不到促进萌发的效果。因此,不同含水量穿心莲种子的萌发对等离子体处理的响应不同,说明含水量是影响处理效果的重要因素。结合前期研究结果,在农业生产中,建议以含水量为11.6%～24.0%的穿心莲种子进行等离子体处理效果最佳。本研究为实际播种前处理和进一步研究等离子体技术在农业生产中的应用提供参考。
In order to find out whether moisture content of Andrographis paniculata seeds could alter the effect of atmospheric pressure air plasma treatment on seed germination,seed moisture contents were adjusted to 6.3%,8.6%,12.9%,18.6%,24.0%,and 33.4%,respectively;then the seeds were treated by air plasma,and germination test was conducted.Germination energy,germination percentage,germination index,radicle length,vigor index,mildew and rotting percentage,and healthy normal seedling percentage were used as germination parameters to evaluate the effect.Results showed that after plasma treatment,seeds with moisture contents from 12.9%to 24.0%germinated faster,the germination percentages and germination indexes were significantly higher than control,and mildew and rotting percentage of seeds or seedlings were decreased,healthy seedling percentage was increased significantly.However,seeds with moisture contents of 6.3%,8.6%and 33.4% did not achieved better germination.Thus,moisture content is an important factor influencing the effects of air plasma on seed germination.Combined with previous results,moisture content of Andrographis paniculataseeds were proposed from 11.6 %to 24.0 %,before plasma treatment.This investigation could provide a reference to pre-sowing treatment and further application studies in plasma agriculture.
In order to find out whether moisture content of Andrographis paniculata seeds could alter the effect of atmospheric pressure air plasma treatment on seed germination,seed moisture contents were adjusted to 6.3%,8.6%,12.9%,18.6%,24.0%,and 33.4%,respectively;then the seeds were treated by air plasma,and germination test was conducted.Germination energy,germination percentage,germination index,radicle length,vigor index,mildew and rotting percentage,and healthy normal seedling percentage were used as germination parameters to evaluate the effect.Results showed that after plasma treatment,seeds with moisture contents from 12.9%to 24.0%germinated faster,the germination percentages and germination indexes were significantly higher than control,and mildew and rotting percentage of seeds or seedlings were decreased,healthy seedling percentage was increased significantly.However,seeds with moisture contents of 6.3%,8.6%and 33.4% did not achieved better germination.Thus,moisture content is an important factor influencing the effects of air plasma on seed germination.Combined with previous results,moisture content of Andrographis paniculataseeds were proposed from 11.6 %to 24.0 %,before plasma treatment.This investigation could provide a reference to pre-sowing treatment and further application studies in plasma agriculture.
引文
[1]孔广红,倪书邦,贺熙勇,等.60 Co-γ射线辐照澳洲坚果种子后的苗期辐射效应[J].核农学报,2018,32(3):417-423.
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[4]erB,erM.Non-thermal plasma treatment as a new biotechnology in relation to seeds,dry fruits,and grains[J].Plasma Science and Technology,2018,20:044012.
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[6]Puligundla P,Kim J-W,Mok C.Effects of nonthermal plasma treatment on decontamination and sprouting of radish(Raphanus sativus L.)seeds[J].Food and Bioprocess Technology,2017,10(6):1093-1102.
[7]Bormashenko E,Grynyov R,Bormashenko Y,et al.Cold radiofrequency plasma treatment modifies wettability and germination speed of plant seeds[J].Scientific Reports,2012,2:741.
[8]Bormashenko E,Shapira Y,Grynyov R,et al.Interaction of cold radiofrequency plasma with seeds of beans(Phaseolus vulgaris)[J].Journal of Experimental Botany,2015,66(13):4013-4021.
[9]Hosseini S I,Mohsenimehr S,Hadian J,et al.Physico-chemical induced modification of seed germination and early development in artichoke(Cynara scolymus L.)using low energy plasma technology[J].Physics of Plasmas,2018,25:013525.
[10]Li L,Li JG,Shen MC,et al.Cold plasma treatment enhances oilseed rape seed germination under drought stress[J].Scientific Reports,2015,5:13033.
[11]Roy N C,Hasan M M,Talukder M R,et al.Prospective applications of low frequency glow discharge plasmas on enhanced germination,growth and yield of wheat[J].Plasma Chemistry and Plasma Processing,2017,38(1):13-28.
[12]Pawlat J,Starek A,Sujak A,et al.Effects of atmospheric pressure plasma generated in GlidArc reactor on Lavatera thuringiaca L.seeds’germination[J].Plasma Processes and Polymers,2017,15(2):e1700064.
[13]Li Y,Wang T,Meng Y,et al.Air atmospheric dielectric barrier discharge plasma induced germination and growth enhancement of wheat seed[J].Plasma Chemistry and Plasma Processing,2017,37(6):1621-1634.
[14]Zhang JJ,Jo JO,Huynh DL,et al.Growth-inducing effects of argon plasma on soybean sprouts via the regulation of demethylation levels of energy metabolism-related genes[J].Scientific Reports,2017,7:41917.
[15]Ji SH,Choi KH,Pengkit A,et al.Effects of high voltage nanosecond pulsed plasma and micro DBD plasma on seed germination,growth development and physiological activities in spinach[J].Archives of Biochemistry and Biophysics,2016,605(1):117-128.
[16]Dubinov A E,Kozhayeva J P,Zuimatch E A.Changing germination rate of brown mustard seeds after treatment with plasmas of nanosecond electric discharges[J].IEEE Transactions on Plasma Science,2017,45(2):294-300.
[17]Stolárik T,HenselováM,Martinka M,et al.Effect of low-temperature plasma on the structure of seeds,growth and metabolism of endogenous phytohormones in pea(Pisum sativum L.)[J].Plasma Chemistry and Plasma Processing,2015,35:659-676.
[18]Gómez-Ramírez A,López-Santos C,Cantos M,et al.Surface chemistry and germination improvement of Quinoa seeds subjected to plasma activation[J].Scientific Reports,2017,7:5924.
[19]erB,erM,Gavril B,et al.Seed germination and early growth responses to seed pre-treatment by non-thermal plasma in hemp cultivars(Cannabis sativa L.)[J].Plasma Chemistry and Plasma Processing,2017,37:207-221.
[20]Zhou RW,Zhou RS,Zhang XH,et al.Effects of atmospheric-pressure N2,He,air,and O2 microplasmas on mung bean seed germination and seedling growth[J].Scientific Reports,2016,6:32603.
[21]Tong JY,He R,Zhang XL,et al.Effects of atmospheric pressure air plasma pretreatment on the seed germination and early growth of Andrographis paniculata[J].Plasma Science and Technology,2014,16(3):260-266.
[22]汤小婷,曾湘达,卓一南,等.等离子体对穿心莲种子萌发、幼苗和植株生长及抗逆性的影响[J].中药新药与临床药理,2017,28(3):385-387.
[23]GB/T 3543.4-1995.农作物种子检验规程.发芽试验[S].1995.
[24]支巨振.《农作物种子检验规程》实施指南[M].北京:中国标准出版社,2000:43-51.
[25]孔德娟,郝丽珍,张凤兰,等.沙芥种子发育过程中的脱水耐性[J].植物生理学报,2014,50(3):324-330.
[26]方莹,周坚.光皮桦超干种子回湿方法研究[J].种子,2007,26(2):36-38.
[27]童家赟,张晓丽,何瑞,等.穿心莲种子发芽试验标准化研究[J].种子,2011,30(2):1-3.
[28]Jiang J,Lu Y,Li J,et al.Effect of seed treatment by cold plasma on the resistance of tomato to Ralstoniasolanacearum(Bacterial Wilt).PLoS ONE,2014,9(5):e97753.
[29]ZahoranováA,HenselováM,HudecováD,et al.Effect of cold atmospheric pressure plasma on the wheat seedlings vigor and on the inactivation of microorganisms on the seeds surface[J].Plasma Chemistry and Plasma Processing,2016,36:397-414.
[30]Hu YY,Zhu WD,Liu K,etal.Influenceofwatercontentonthe inactivation of P.digitatum spores using an air-water plasma jet[J].Plasma Science and Technology,2018,20:044011.
[31]Zhang ZL,Shen J,Cheng C,et al.Generation of reactive species in atmospheric pressure dielectric barrier discharge with liquid water[J].Plasma Science and Technology,2018,20:044009.
[32]何瑞,童家赟,张晓丽,等.大气压等离子体处理对穿心莲种子萌发及幼苗的影响初报[J].广东农业科学,2011,38(16):23-25.
[2]李俊,张西西,董爱香,等.离子注入一串红的生物学效应研究[J].核农学报,2015,29(7):1302-1306.
[3]Pua cˇN,Gherardi M,Shiratani M.Plasma agriculture:A rapidly emerging field[J].Plasma Processes and Polymers,2017:e1700174.
[4]erB,erM.Non-thermal plasma treatment as a new biotechnology in relation to seeds,dry fruits,and grains[J].Plasma Science and Technology,2018,20:044012.
[5]Puligundla P,Kim J-W,Mok C.Effect of corona discharge plasma jet treatment on decontamination and sprouting of rapeseed(Brassica napus L.)seeds[J].Food Control,2017,71:376-382.
[6]Puligundla P,Kim J-W,Mok C.Effects of nonthermal plasma treatment on decontamination and sprouting of radish(Raphanus sativus L.)seeds[J].Food and Bioprocess Technology,2017,10(6):1093-1102.
[7]Bormashenko E,Grynyov R,Bormashenko Y,et al.Cold radiofrequency plasma treatment modifies wettability and germination speed of plant seeds[J].Scientific Reports,2012,2:741.
[8]Bormashenko E,Shapira Y,Grynyov R,et al.Interaction of cold radiofrequency plasma with seeds of beans(Phaseolus vulgaris)[J].Journal of Experimental Botany,2015,66(13):4013-4021.
[9]Hosseini S I,Mohsenimehr S,Hadian J,et al.Physico-chemical induced modification of seed germination and early development in artichoke(Cynara scolymus L.)using low energy plasma technology[J].Physics of Plasmas,2018,25:013525.
[10]Li L,Li JG,Shen MC,et al.Cold plasma treatment enhances oilseed rape seed germination under drought stress[J].Scientific Reports,2015,5:13033.
[11]Roy N C,Hasan M M,Talukder M R,et al.Prospective applications of low frequency glow discharge plasmas on enhanced germination,growth and yield of wheat[J].Plasma Chemistry and Plasma Processing,2017,38(1):13-28.
[12]Pawlat J,Starek A,Sujak A,et al.Effects of atmospheric pressure plasma generated in GlidArc reactor on Lavatera thuringiaca L.seeds’germination[J].Plasma Processes and Polymers,2017,15(2):e1700064.
[13]Li Y,Wang T,Meng Y,et al.Air atmospheric dielectric barrier discharge plasma induced germination and growth enhancement of wheat seed[J].Plasma Chemistry and Plasma Processing,2017,37(6):1621-1634.
[14]Zhang JJ,Jo JO,Huynh DL,et al.Growth-inducing effects of argon plasma on soybean sprouts via the regulation of demethylation levels of energy metabolism-related genes[J].Scientific Reports,2017,7:41917.
[15]Ji SH,Choi KH,Pengkit A,et al.Effects of high voltage nanosecond pulsed plasma and micro DBD plasma on seed germination,growth development and physiological activities in spinach[J].Archives of Biochemistry and Biophysics,2016,605(1):117-128.
[16]Dubinov A E,Kozhayeva J P,Zuimatch E A.Changing germination rate of brown mustard seeds after treatment with plasmas of nanosecond electric discharges[J].IEEE Transactions on Plasma Science,2017,45(2):294-300.
[17]Stolárik T,HenselováM,Martinka M,et al.Effect of low-temperature plasma on the structure of seeds,growth and metabolism of endogenous phytohormones in pea(Pisum sativum L.)[J].Plasma Chemistry and Plasma Processing,2015,35:659-676.
[18]Gómez-Ramírez A,López-Santos C,Cantos M,et al.Surface chemistry and germination improvement of Quinoa seeds subjected to plasma activation[J].Scientific Reports,2017,7:5924.
[19]erB,erM,Gavril B,et al.Seed germination and early growth responses to seed pre-treatment by non-thermal plasma in hemp cultivars(Cannabis sativa L.)[J].Plasma Chemistry and Plasma Processing,2017,37:207-221.
[20]Zhou RW,Zhou RS,Zhang XH,et al.Effects of atmospheric-pressure N2,He,air,and O2 microplasmas on mung bean seed germination and seedling growth[J].Scientific Reports,2016,6:32603.
[21]Tong JY,He R,Zhang XL,et al.Effects of atmospheric pressure air plasma pretreatment on the seed germination and early growth of Andrographis paniculata[J].Plasma Science and Technology,2014,16(3):260-266.
[22]汤小婷,曾湘达,卓一南,等.等离子体对穿心莲种子萌发、幼苗和植株生长及抗逆性的影响[J].中药新药与临床药理,2017,28(3):385-387.
[23]GB/T 3543.4-1995.农作物种子检验规程.发芽试验[S].1995.
[24]支巨振.《农作物种子检验规程》实施指南[M].北京:中国标准出版社,2000:43-51.
[25]孔德娟,郝丽珍,张凤兰,等.沙芥种子发育过程中的脱水耐性[J].植物生理学报,2014,50(3):324-330.
[26]方莹,周坚.光皮桦超干种子回湿方法研究[J].种子,2007,26(2):36-38.
[27]童家赟,张晓丽,何瑞,等.穿心莲种子发芽试验标准化研究[J].种子,2011,30(2):1-3.
[28]Jiang J,Lu Y,Li J,et al.Effect of seed treatment by cold plasma on the resistance of tomato to Ralstoniasolanacearum(Bacterial Wilt).PLoS ONE,2014,9(5):e97753.
[29]ZahoranováA,HenselováM,HudecováD,et al.Effect of cold atmospheric pressure plasma on the wheat seedlings vigor and on the inactivation of microorganisms on the seeds surface[J].Plasma Chemistry and Plasma Processing,2016,36:397-414.
[30]Hu YY,Zhu WD,Liu K,etal.Influenceofwatercontentonthe inactivation of P.digitatum spores using an air-water plasma jet[J].Plasma Science and Technology,2018,20:044011.
[31]Zhang ZL,Shen J,Cheng C,et al.Generation of reactive species in atmospheric pressure dielectric barrier discharge with liquid water[J].Plasma Science and Technology,2018,20:044009.
[32]何瑞,童家赟,张晓丽,等.大气压等离子体处理对穿心莲种子萌发及幼苗的影响初报[J].广东农业科学,2011,38(16):23-25.
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