鹅源H5N1禽流感病毒感染对雏鸭红细胞免疫功能的影响
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摘要
将100只21日龄雏鸭随机分为AIV感染组(I)和对照组(C),应用免疫学和细胞培养及MTT等方法动态检测AIV感染雏鸭血液红细胞免疫黏附能力、红细胞免疫自身调控能力以及红细胞促外周血淋巴细胞增殖能力。结果表明,雏鸭感染AIV后1～14 d,红细胞C_(3b)受体花环(E-CRR)率明显低于对照组,而红细胞免疫复合物花环(E-ICR)率明显高于对照组;AIV感染雏鸭后3～21 d,红细胞C_(3b)受体花环促进率(RFER)显著低于对照组,且红细胞C_(3b)受体花环抑制(RFIR)率显著高于对照组;AIV感染雏鸭的红细胞对血液T、B淋巴细胞增殖促进能力明显低于对照组。表明AIV感染引起的雏鸭红细胞免疫黏附功能、自身调控能力和T、B淋巴细胞增殖能力下降,与AIV感染导致雏鸭外周血免疫功能降低密切相关。
In this study, one hundred 21-day-old ducklings were randomly assigned into two groups: AIV infection group(I) and un-infection group(C). Erythrocyte immune adherence function,erythrocyte immune self-regulation ability and regulative ability of erythrocyte acting on lymphocyte proliferation were performed to figure out the mechanism of duckling immune pathogenesis with MTT method. The results showed that erythrocyte C_(3b) receptor rosette(E-CRR)ratio of duckling infected with AIV was significantly decreased than that AIV un-infection ducklings in 1-14 d. erythrocyte immune complex rosette(E-ICR) ratio of AIV infection group was significantly higher than that of control group in1-14 d; the immune enhancing factor of AIV infection ducklings were lower than that in control group from 3-21 d, while the immune inhibition factor of AIV infection group were higher from 3-21 d than that in control group; Besides, the ability of T, B lymphocytes proliferation in the AIV infection group was obviously lower than that in control group. Therefore, this study illustrated that the AIV infectioninduced declines of erythrocyte immune adherence function, self-regulation ability and T, B lymphocyte proliferation ability in ducklings are closely related to erythrocyte immune.
In this study, one hundred 21-day-old ducklings were randomly assigned into two groups: AIV infection group(I) and un-infection group(C). Erythrocyte immune adherence function,erythrocyte immune self-regulation ability and regulative ability of erythrocyte acting on lymphocyte proliferation were performed to figure out the mechanism of duckling immune pathogenesis with MTT method. The results showed that erythrocyte C_(3b) receptor rosette(E-CRR)ratio of duckling infected with AIV was significantly decreased than that AIV un-infection ducklings in 1-14 d. erythrocyte immune complex rosette(E-ICR) ratio of AIV infection group was significantly higher than that of control group in1-14 d; the immune enhancing factor of AIV infection ducklings were lower than that in control group from 3-21 d, while the immune inhibition factor of AIV infection group were higher from 3-21 d than that in control group; Besides, the ability of T, B lymphocytes proliferation in the AIV infection group was obviously lower than that in control group. Therefore, this study illustrated that the AIV infectioninduced declines of erythrocyte immune adherence function, self-regulation ability and T, B lymphocyte proliferation ability in ducklings are closely related to erythrocyte immune.
引文
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[18]张静静,王春,贾瑞璞,等.猪红细胞免疫黏附功能与CR1-like表达水平的研究[J].中国兽医科学,2018,48(5):593-604.
[19]匡宝晓,徐福南.网状内皮组织增殖病病毒感染鸡红细胞免疫功能的动态变化[J].中国兽医科技,1997(11):7-9.
[20]左之才,崔恒敏,甘梦,等.感染猪放线杆菌(Ⅰ型)对猪红细胞免疫功能的影响[J].东北农业大学学报,2012,43(12):21-25.
[21]田伟,仇铮,刘明,等.雏鸭感染H5N1亚型禽流感病毒后主要组织器官超微病理变化及病毒抗原分布的动态变化研究[J].中国兽医科学,2018,48(5):641-648.
[22]杨沛,陈伟,龚敏,等.高压氧治疗后红细胞免疫功能的变化及其临床意义[J].中国现代医学杂志,2003(6):14-16.
[23]郭峰,钱宝华,许育,等.红细胞天然免疫功能及其测定方法[J].现代医学仪器与应用,2003(2):1-4.
[2]姜慧,赖圣杰,秦颖,等.全球人感染禽流感疫情及其流行病学特征概述[J].科学通报,2017,62(19):2104-2115.
[3]Freidl G S,Meijer A,de Bruin E,et al.Influenza at the animalhuman interface:A review of the literature for virological evidence of human infection with swine or avian influenza viruses other than a(H5N1)[J].Euro Surveill,2014,19(18):8-26.
[4]刘广红,郭永祥,邱立新.家鸭禽流感的变化特征及防控对策[J].中国畜牧兽医,2008(3):91-93.
[5]Siegel I,Liu T L,Gleicher N.The red-cell immune system[J].Lancet,1981,318(8246):556-559.
[6]王灵娟,郑世民.禽类红细胞免疫功能与疾病研究进展[J].动物医学进展,2012,33(3):84-88.
[7]St Paul M,Paolucci S,Barjesteh N,et al.Chicken erythrocytes respond to Toll-like receptor ligands by up-regulating cytokine transcripts[J].Research in Veterinary Science,2013,95(1):87-91.
[8]孟繁思.益生菌对雏鸡红细胞免疫和氧化-抗氧化功能的影响[D].哈尔滨:东北农业大学,2014.
[9]马宏伟,郑世民,赵良友,等.红细胞免疫研究进展[J].中国兽医杂志,2009,45(4):79-80.
[10]Meabed M H,Taha G M,Mohamed S O,et al.Autoimmune thrombocytopenia:Flow cytometric determination of platelet-associated CD154/CD40L and CD40 on peripheral blood T and B lymphocytes[J].Hematology,2007,12(4):301-307.
[11]Arosa F A.Red blood cells as modulators of T cell growth and survival[J].Current Pharmaceutical Design,2004,10(2):191-201.
[12]杨尚琪,许予明.小鼠红细胞对脾细胞增殖反应的影响[J].中华血液学杂志,1994(5):258-259.
[13]郑世民,王滨坤,高雪丽,等.鹅源H5N1亚型禽流感病毒对SPF雏鸡血液免疫功能的影响[J].东北农业大学学报,2011,42(12):69-73.
[14]于学武,吕晓萍,刘超男,等.雏鸭感染鹅源H5N1亚型禽流感病毒后免疫器官T细胞免疫功能及IL-2 mRNA转录变化[J].畜牧兽医学报,2014,45(9):1497-1504.
[15]Virella G,Rugeles M T,Hyman B,et al.The interaction of CD2with its LFA-3 ligand expressed by autologous erythrocytes results in enhancement of B cell responses[J].Cellular Immunology,1988,116(2):308-319.
[16]佟巍,高红,蒋虹,等.H5N1接种恒河猴和食蟹猴后外周血细胞变化的分析[J].中国比较医学杂志,2009,19(1):1-4.
[17]孔祥瑞,石炳毅.红细胞在移植免疫中作用的研究进展[J].中华器官移植杂志,2014,35(10):634-636.
[18]张静静,王春,贾瑞璞,等.猪红细胞免疫黏附功能与CR1-like表达水平的研究[J].中国兽医科学,2018,48(5):593-604.
[19]匡宝晓,徐福南.网状内皮组织增殖病病毒感染鸡红细胞免疫功能的动态变化[J].中国兽医科技,1997(11):7-9.
[20]左之才,崔恒敏,甘梦,等.感染猪放线杆菌(Ⅰ型)对猪红细胞免疫功能的影响[J].东北农业大学学报,2012,43(12):21-25.
[21]田伟,仇铮,刘明,等.雏鸭感染H5N1亚型禽流感病毒后主要组织器官超微病理变化及病毒抗原分布的动态变化研究[J].中国兽医科学,2018,48(5):641-648.
[22]杨沛,陈伟,龚敏,等.高压氧治疗后红细胞免疫功能的变化及其临床意义[J].中国现代医学杂志,2003(6):14-16.
[23]郭峰,钱宝华,许育,等.红细胞天然免疫功能及其测定方法[J].现代医学仪器与应用,2003(2):1-4.
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