香菇多糖对猪瘟疫苗免疫效果的影响

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英文篇名：Effect of lentinus edodes polysaccharide on immune response of classical swine fever 作者：阳玉彪 ; 唐桂华 ; 郑明愈 ; 凌丁 ; 李军成 ; 莫文湛 ; 胡传水 ; 熊飙 ; 杨柳 英文作者：Yang Yu-biao;Tang Gui-hua;Zheng Ming-yu;Ling Ding;Li Jun-cheng;Mo Wen-zhan;Hu Chuan-shui;Xiong Biao;Yang Liu;Guangxi Agricultural Vocational and Technical College;Lyu Jian-da Pig Farm,Heng County; 关键词：香菇多糖 ; 猪瘟疫苗 ; 免疫效果 英文关键词：lentinan;;swine fever vaccine;;immune effect 中文刊名：广西农学报 英文刊名：Journal of Guangxi Agriculture 机构：广西农业职业技术学院;广西横县绿健达养猪场; 出版日期：2019-02-20 出版单位：广西农学报 年：2019 期：01 基金：2017年度广西高校中青年教师基础能力提升项目“香菇多糖对猪瘟疫苗免疫效果的研究”(2017KY1136) 语种：中文; 页：43-46+66 页数：5 CN：45-1133/S ISSN：1003-4374 分类号：S858.28

摘要

【目的】为了进一步提高猪瘟疫苗的免疫效果,探讨香菇多糖对猪瘟疫苗的免疫增强作用,为新型猪瘟疫苗免疫增强剂的研发提供理论依据。【方法】以不同剂量的香菇多糖作为猪瘟疫苗的稀释液免疫断奶仔猪,分别于免疫当天、免疫接种后第7、14、21、28d无菌操作采集血样,运用ELISA检测血清猪瘟抗体水平。【结果】香菇多糖可显著提高血清中的猪瘟抗体水平,第4组(0.1875mg香菇多糖)于免疫后14d和28d,猪瘟抗体阻断率平均值分别比猪瘟疫苗专用稀释液稀释组提高13.6%和12.2%;免疫合格率为100%。【结论】实验结果表明,将香菇多糖作为猪瘟疫苗稀释液稀释,可特异性提高猪体的免疫应答,香菇多糖具有显著增强猪瘟疫苗免疫效果的作用。
        【Objective】 To further improve the immune effect of the swine fever vaccine,to study the immunopotentiation effect of the lentinan on the swine fever vaccine,and to provide the theoretical basis for the development of the novel swine fever vaccine immunopotentiator.【Method】 To immunize weaned piglets with different doses of lentinan as the diluent of the swine fever vaccine,the blood samples were collected at the 7 th,14 th,21 st and 28 th day after the immunization,and the level of the serum hog cholera antibody was detected by ELISA.【Result】 Lentinan could significantly improve the level of the swine fever antibody in the serum,and the fourth group(0.1875 mg of lentinan) was 14 days after the immunization and 28 days after the immunization.The average value of the antibody blocking rate of the swine fever was 13.6% and 12.2%,respectively,compared with that of the special diluent of the swine fever vaccine,and the rate of the immune qualification rate was 100%.【Conclusion】 The results of the experiment show that the lentinan is used as the diluent of the swine fever vaccine,and can improve the immune response of the pig body,and the lentinan has the effect of significantly enhancing the immune effect of the swine fever vaccine.

引文

[1]李娇,王金良,沈志强,等.荧光定量PCR检测方法在猪瘟诊断上的应用研究进展[J].养猪,2012(6):98-101.
    [2]张彦广,吴予君,李辉,等.关于猪瘟防控的体会[J].中国畜禽种业,2013(2):34-35.
    [3]沈志强.猪瘟疫苗的研究概况、选择及科学使用[J].饲料与畜牧(规模养猪),2013(2):37-41.
    [4]仇华吉.猪瘟疫苗的现在和将来[J].河南畜牧兽医,2012,33(6):33-34.
    [5]王琴,范学政,赵启祖,等.猪瘟防控技术研究进展[J].中国兽药杂志,2012,47(9):58-61.
    [6]于攀顺.猪瘟免疫失败原因浅析[J].动物医学进展,2011,32(10):117-120.
    [7]蔡碧玉.免疫增强剂对猪瘟疫苗免疫效果的研究[J].畜禽业,2014(01):52-53.
    [8]朱俊访,李博.香菇多糖提取工艺的研究进展[J].中国民族民间医药,2013(1):43.
    [9]施伟领,徐慧,叶秀娟,等.香菇多糖对鸡球虫3-1E重组蛋白的免疫增强作用[J].中国兽医学报,2009,29(11):1444-1449.