冀北坝上不同土地利用对土壤微生物量碳氮磷及酶活性的影响
|
摘要
为了研究冀北坝上不同土地利用对土壤微生物量及酶活性的影响,揭示土地利用方式对土壤肥力及微生物的影响机制,在冀北坝上选取草地、林地及多种不同农用地(露地菜地、青贮玉米地、大棚菜地、马铃薯地和燕麦地),采集表层(0-20cm)土壤,分析土壤微生物量及酶活性的差异。结果表明,不同土地利用类型土壤微生物量碳(BC)为49.15-425.20 mg·kg~(-1),土壤微生物量氮(BN)为9.89-64.08 mg·kg~(-1),土壤微生物量磷(BP)为0.50-13.10 mg·kg~(-1);脲酶活性为0.01-0.08 mg·g~(-1)·h~(-1),土壤蔗糖酶活性为0.11-0.18mg·g~(-1)·h~(-1)。其中草地土壤BC、BN及酶活性高于林地和农用地,林地土壤BP高于草地和农用地。在不同农用地土壤中,大棚菜地土壤BC、BN较高,青贮玉米地土壤BP较高而BC较低,露地菜地土壤BN、BP较低而酶活性较强。BC与总有机质(TOM)、总氮(TN)、总磷(TP)、氨氮(NH_4~+-N)均呈显著正相关,BP与活性有机质(ASOM)呈极显著正相关,与无机磷(IP)、IP/TP呈显著负相关,脲酶与TN、TP、ASOM/TOM、蔗糖酶活性均呈显著正相关。针对冀北坝上贫瘠、退化严重土壤可通过改种牧草进行改良,针对酶活性较低、肥力差的土壤可通过增施有机肥进行调节,在部分地区建立设施农业以提高土壤微生物量,种植归还率较高的作物防止农用地土壤退化。
To study the effects of different land uses on soil microbial biomass and enzyme activities in the plateau of north Hebei,and to reveal the influence mechanism of land use type on soil fertility and microorganism. Grassland, woodland and a variety of different agricultural land(open field vegetable field, silage corn field, greenhouse vegetable field, potato field and oat field) were selected in the plateau of north Hebei, and surface(0-20 cm) soil was collected to analyze the differences in soil microbial biomass and enzyme activity. The results showed that the soil microbial biomass carbon(BC), soil microbial biomass nitrogen(BN), soil microbial biomass phosphorus(BP) content ranged from 49.15, 9.89, 0.50 mg·kg~(-1) to 425.20, 64.08, 13.10 mg·kg~(-1), respectively.Soil urease activity and invertase activity ranged from 0.01, 0.11 mg·g~(-1)·h-1 to 0.08, 0.18 mg·g~(-1)·h-1 respectively in different land use types. The contents of BC, BN and the activity of enzyme in grassland were higher than those in woodland and farmland. The content of BP in woodland was higher than that in grassland and farmland. For farmland with different vegetation, the contents of BC and BN in greenhouse vegetable soil were higher. The content of BP in silage maize soil was higher, but the BC content was lower. The contents of BN and BP in open vegetable soil was lower, while the enzyme activity was stronger. The content of BC was positively correlated with the activity of total organic matter(TOM), total nitrogen(TN), total phosphorus(TP) and NH_4~+-N significantly. The content of BP was significant positively correlated with(active soil organic matter) ASOM, while negatively correlated with inorganic phosphorus(IP) and IP/TP content. Urease activity was positively correlated with TN, TP, ASOM/TOM content and invertase activity. According to the result in this paper, the poor and degraded soils can be improved by replanting pasture, and the application of organic fertilizer can be regulated for the soils with low enzyme activity and poor fertility soil. Facility agriculture can be established in some areas to improve soil microbial biomass and plant crops with high return rate to prevent soil degradation.
To study the effects of different land uses on soil microbial biomass and enzyme activities in the plateau of north Hebei,and to reveal the influence mechanism of land use type on soil fertility and microorganism. Grassland, woodland and a variety of different agricultural land(open field vegetable field, silage corn field, greenhouse vegetable field, potato field and oat field) were selected in the plateau of north Hebei, and surface(0-20 cm) soil was collected to analyze the differences in soil microbial biomass and enzyme activity. The results showed that the soil microbial biomass carbon(BC), soil microbial biomass nitrogen(BN), soil microbial biomass phosphorus(BP) content ranged from 49.15, 9.89, 0.50 mg·kg~(-1) to 425.20, 64.08, 13.10 mg·kg~(-1), respectively.Soil urease activity and invertase activity ranged from 0.01, 0.11 mg·g~(-1)·h-1 to 0.08, 0.18 mg·g~(-1)·h-1 respectively in different land use types. The contents of BC, BN and the activity of enzyme in grassland were higher than those in woodland and farmland. The content of BP in woodland was higher than that in grassland and farmland. For farmland with different vegetation, the contents of BC and BN in greenhouse vegetable soil were higher. The content of BP in silage maize soil was higher, but the BC content was lower. The contents of BN and BP in open vegetable soil was lower, while the enzyme activity was stronger. The content of BC was positively correlated with the activity of total organic matter(TOM), total nitrogen(TN), total phosphorus(TP) and NH_4~+-N significantly. The content of BP was significant positively correlated with(active soil organic matter) ASOM, while negatively correlated with inorganic phosphorus(IP) and IP/TP content. Urease activity was positively correlated with TN, TP, ASOM/TOM content and invertase activity. According to the result in this paper, the poor and degraded soils can be improved by replanting pasture, and the application of organic fertilizer can be regulated for the soils with low enzyme activity and poor fertility soil. Facility agriculture can be established in some areas to improve soil microbial biomass and plant crops with high return rate to prevent soil degradation.
引文
CALBRIX R,BARRAY S,CHABRERIE O,et al.,2007.Impact of organic amendments on the dynamics of soil microbial biomass and bacterial communities in cultivated land[J].Applied Soil Ecology,35(3):511-522.
CLEVELAND C C,LIPTZIN D,2007.C-N-P stoichiometry in soil:is there a“Redfield ratio”for the microbial biomass?[J].Biogeochemistry,85(3):235-252.
KUMAR B T N,BABALAD H B,2018.Soil Organic Carbon,Carbon Sequestration,Soil microbial biomass carbon and nitrogen and soil enzymatic activity as influenced by conservation agriculture in pigeonpea and soybean intercropping system[J].International Journal of Current Microbiology and Applied Sciences,7(3):323-333.
LI D P,WU Z J,CHEN L J,2004.Dynamics of microbial biomass C in a black soil under long-term fertilization and related affecting factors[J].Chinese Journal of Applied Ecology,15(8):1334-1338.
NSABIMANA D,HAYNES R J,WALLIS F M,2004.Size,activity and catabolic diversity of the soil microbial biomass as affected by land use[J].Applied Soil Ecology,26:81-82.
ROD D B L,GRAEME J B,WAYNE M S,1993.Changes in soil organic matter with cropping as measured by organic carbon fractions and 13 Cnatural isotope abundance[J].Plant and Soil,155-156(1):399-402.
ROS G H,TEMMINGHOFF E J M,GROENIGEN J W V,et al.,2010.Isotopic analysis of dissolved organic nitrogen in soils[J].Analytical Chemistry,82(18):7814-7820.
XU X F,THORNTON P E,POST W M,2013.A global analysis of soil microbial biomass carbon,nitrogen and phosphorus in terrestrial ecosystems[J].Global Ecology and Biogeography,22(5-6):737-749.
宝日玛,峥嵘,周梅,等,2016.大兴安岭火烧迹地土壤微生物生物量及酶活性研究[J].内蒙古农业大学学(自然科学版),37(4):77-83.BAO R A,ZHENG R,ZHOU M,et al.,2016.Soil microbial biomass and enzyme activity of burned sites in greater hinggan mountains[J].Science of Inner Mongolia Agricultural University(Natural Science Edition),37(4):77-83.
鲍士旦,2000.土壤农化分析[M].3版.北京:中国农业出版社:25-96.BAO S D,2000.Soil agrochemical analysis[M].3 Ed.Beijing:China agriculture Press:25-96.
仇少君,彭佩钦,刘强,等,2006.土壤微生物生物量氮及其在氮素循环中作用[J].生态学杂志,25(4):443-448.QIU S J,PENG P Q,LIU Q,et al.,2006.Soil microbial biomass nitrogen and its role in nitrogen cycle[J].Ecology,25(4):443-448.
董莉丽,郑粉莉,安娟,2010.黄土丘陵不同土地利用类型下土壤微生物量特征[J].土壤通报,41(6):1371-1375.DONG L L,ZHENG F L,AN J,2010.Characteristics of soil microbial biomass under different land use types in loess hills[J].Chinese Soil Bulletin,41(6):1371-1375.
关松阴,1986.土壤酶及其研究方法[M].北京:农业出版社.GUAN S Y,1986.Soil enzyme and its research methods[M].Beijing:Agricultural Press.
韩永伟,韩建国,张蕴薇,等,2004.农牧交错带草地植被的水土保持作用研究[J].水土保持学报,18(4):24-28.HAN Y W,HAN J G,ZHANG Y W,et al.,2004.Study on soil and water conservation of grassland vegetation in farming-pastoral ecotone[J].Journal of Soil and Water Conservation,18(4):24-28.
贺美,王立刚,朱平,等,2017.长期定位施肥下黑土碳排放特征及其碳库组分与酶活性变化[J].生态学报,37(19):6379-6389.HE M,WANG L G,ZHU P,et al.,2017.Carbon emission characteristics,carbon sink components and enzyme activities of black soil under long-term fertilization[J].Acta Ecologica Sinica,37(19):6379-6389.
黄智鸿,赵海超,魏东,等,2016.不同施肥措施对春玉米农用地土壤酶活性的影响[J].中国土壤与肥料,265(5):18-24.HUANG Z H,ZHAO H C,WEI D,et al.,2016.Effects of different fertilization measures on soil enzyme activities in spring maize agricultural land[J].China Soil and Fertilizer,265(5):18-24.
霍利霞,红梅,赵巴音那木拉,等,2018.氮素和水分添加对荒漠草原土壤理化性质及微生物量碳氮的影响[J].北方农业学报,46(3):54-59.HUO L X,HONG M,ZHAO B Y N M L,et al.,2018.Effects of nitrogen and water addition on soil physical and chemical properties and microbial biomass of carbon and nitrogen in desert steppe[J].Journal of Northern Agriculture,46(3):54-59.
蒋永梅,师尚礼,田永亮,等,2017.高寒草地不同退化程度下土壤微生物及土壤酶活性变化特征[J].水土保持学报,31(3):244-249.JIANG Y M,SHI S L,TIAN Y L,et al.,2017.Changes of soil microorganism and soil enzyme activity in alpine grassland under different degrees of degradation[J].Journal of Soil and Water Conservation,31(3):244-249.
李生宝,王占军,王月玲,2006.宁南山区不同生态恢复措施对土壤环境效应影响的研究[J].水土保持学报,20(4):20-22.LI S B,WANG Z J,WANG Y L,2006.Study on effects of different ecological restoration measures on soil environment in ningnan mountain area[J].Journal of Soil and Water Conservation,20(4):20-22.
李亚娟,刘静,徐长林,等,2018.不同退化程度对高寒草甸土壤无机氮及脲酶活性的影响[J].草业学报,27(10):45-53.LI Y J,LIU J,XU C L,et al.,2018.Effects of different degrees of degradation on soil inorganic nitrogen and urease activity in alpine meadow[J].Journal of Grass Industry,27(10):45-53.
刘靓,2017.有机物料对复垦土壤有机碳含量及微生物活性的影响[D].太原:山西大学.LIU L,2017.Effects of organic materials on organic carbon content and microbial activity in reclaimed soil[D].Taiyuan:Shanxi University.
刘霓红,蒋先平,程俊峰,2018.国外有机设施园艺现状及对中国设施农业可持续发展的启示[J].农业工程学报,34(15):1-9.LIU N H,JIANG X P,CHENG J F,2018.The status quo of foreign organic facility horticulture and its enlightenment to the sustainable development of China's facility agriculture[J].Journal of Agricultural Engineering,34(15):1-9.
刘杨,2018.河南省森林土壤及微生物碳、氮、磷的生态化学计量特征[D].洛阳:河南科技大学.LIU Y,2018.Ecological stoichiometric characteristics of carbon,nitrogen and phosphorus in forest soil and microorganisms in henan province[D].Luoyang:Henan University of Science and Technology.
刘正恩,2010.河北坝上生态退化现状、原因及对策措施[J].生态经济(1):166-169.LIU Z E,2010.The current situation,causes of the ecological degradation in Bashang area of Hebei province and the countermeasures on them[J].Ecological Economics(1):166-169.
鲁如坤,1999.土壤农业化学分析方法[M].北京:中国农业科技出版社:106-110.LU L K,1999.Soil chemical analysis methods[M].Beijing:China Agricultural Science and Technology Press:106-110.
宁川川,王建武,蔡昆争,2016.有机肥对土壤肥力和土壤环境质量的影响研究进展[J].生态环境学报,25(1):175-181.NING C C,WANG J W,CAI K Z,2016.Research progress on the effect of organic fertilizers on soil fertility and soil environmental quality[J].Journal of Ecological Environment,25(1):175-181.
萐薛,李占斌,李鹏,等,2011.不同土地利用方式对干热河谷地区土壤酶活性的影响[J].中国农业科学,44(18):3768-3777.SHA X,LI Z B,LI P,et al.,2011.Effects of different land use patterns on soil enzyme activities in hot and dry river valleys[J].China Agricultural Science,44(18):3768-3777.
唐玉霞,贾树龙,孟春香,等,2002.土壤微生物生物量氮研究综述[J].中国生态农业学报,10(2):76-78.TANG Y X,JIA S L,NENG X C,et al.,2002.Review of soil microbial biomass nitrogen research[J].Chinese Journal of Ecological Agriculture,10(2):76-78.
王传杰,王齐齐,徐虎,等,2018.长期施肥下农田土壤-有机质-微生物的碳氮磷化学计量学特征[J].生态学报,38(11):3848-3858.WANG C J,WANG Q Q,XU H,et al.,2018.Carbon,nitrogen and phosphorus stoichiometric characteristics of soil-organic mattermicroorganisms under long-term fertilization[J].Acta Ecologica Sinica,38(11):3848-3858.
王丰,2008.武夷山不同海拔植被带土壤微生物量碳、氮、磷研究[D].南京:南京林业大学.WANG F,2008.Soil microbial biomass of carbon,nitrogen and phosphorus in vegetation zones at different elevations in wuyi mountain[D].Nanjing:Nanjing Forestry University.
王石英,蔡强国,吴淑安,2004.中国北方农牧交错区研究展望[J].水土保持研究,11(4):138-142.WANG S Y,CAI Q G,WU S A,2004.Prospects of agro-pastoral ecotone in north China[J].Research on Soil and Water Conservation,11(4):138-142.
王文锋,李春花,黄绍文,等,2016.不同施肥模式对设施菜田土壤微生物量碳、氮的影响[J].植物营养与肥料学报,22(5):1286-1297.WANG W F,LI C H,HUANG S W,et al.,2016.Effects of different fertilization modes on soil microbial biomass carbon and nitrogen in vegetable field[J].Chinese Journal of Plant Nutrition and Fertilizer,22(5):1286-1297.
王月,刘兴斌,韩晓日,等,2016.不同施肥处理对连作花生土壤微生物量和酶活性的影响[J].沈阳农业大学学报,47(5):553-558.WANG Y,LIU W B,HAN X R,et al.,2016.Effects of different fertilization treatments on soil microbial biomass and enzyme activity of continuous cropping peanut[J].Journal of Shenyang Agricultural University,47(5):553-558
吴金水,林启美,黄巧云,等,2006.土壤微生物量测定方法及应用[M].北京:气象出版社.WU J S,LIN Q M,HUANG Q Y,et al.,2006.Soil microbial biomass determination and its application[M].Beijing:Meteorological Press.
杨滨娟,黄国勤,钱海燕,2014.秸秆还田配施化肥对土壤温度、根际微生物及酶活性的影响[J].土壤学报,51(1):151-157.YANG B J,HUANG G Q,QIAN H Y,2014.Effects of straw mulching and fertilizer application on soil temperature,rhizosphere microorganism and enzyme activity[J].Acta Edologica Sinica,51(1):151-157.
杨雪艳,蒋代华,杨钙仁,等,2018.甘蔗水肥一体化种植对土壤微生物量碳氮和酶活性的影响[J].土壤通报,49(4):889-896.YANG X Y,JIANG D H,YANG G R,et al.,2018.Effects of sugarcane water and fertilizer integrated planting on soil microbial biomass,carbon and nitrogen and enzyme activities[J].Chinese Soil Science Bulletin,49(4):889-896.
尹亚丽,王玉琴,鲍根生,等,2017.退化高寒草甸土壤微生物及酶活性特征[J].应用生态学报,28(12):3881-3890.YIN Y L,WANG Y Q,BAO G S,et al.,2017.Characteristics of soil microorganism and enzyme activity in degraded alpine meadow[J].Chinese Journal of Applied Ecology,28(12):3881-3890.
张娟霞,刘伟刚,宁媛,等,2018.长期秸秆还田与施氮后土壤活性碳、氮的变化[J].水土保持学报,32(3):212-217.ZHANG J X,LIU W G,NING Y,et al.,2018.Changes of soil active carbon and nitrogen after long-term straw mulching and nitrogen application[J].Journal of Soil and Water Conservation,32(3):212-217.
张新荣,刘林萍,方石,等,2014.土地利用、覆被变化(LUCC)与环境变化关系研究进展[J].生态环境学报,23(12):2013-2021.ZHANG X R,LIU L P,FANG S,et al.,2014.Research progress on the relationship between land use,cover change(LUCC)and environmental change[J].Journal of Ecological Environment,23(12):2013-2021.
张知晓,泽桑梓,户连荣,等,2018.土壤脲酶活性调控因素和脲酶活性细菌系统发育研究[J].西部林业科学,47(1):65-73.ZHANG Z X,ZE S Z,HU L R,et al.,2018.Study on soil urease activity regulation factors and bacterial phylogeny of urease activity[J].Western Forestry Science,47(1):65-73.
赵海超,刘景辉,张星杰,等,2013.施肥对不同肥力水平春玉米农用地土壤有机碳及其组分的影响[J].生态环境学报,22(3):398-405.ZHAO H C,LIU J H,ZHANG X J,et al.,2013.Effects of fertilization on soil organic carbon and its components in spring maize agricultural land with different fertility levels[J].Journal of Ecological Environment,22(3):398-405.
赵彤,闫浩,蒋跃利,等,2013.黄土丘陵区植被类型对土壤微生物量碳氮磷的影响[J].生态学报,33(18):5615-5622.ZHAO T,YAN H,JIANG Y L,et al.,2013.Effects of vegetation types on soil microbial biomass of carbon,nitrogen and phosphorus in loess hilly regions[J].Acta Ecologica Sinica,33(18):5615-5622.
CLEVELAND C C,LIPTZIN D,2007.C-N-P stoichiometry in soil:is there a“Redfield ratio”for the microbial biomass?[J].Biogeochemistry,85(3):235-252.
KUMAR B T N,BABALAD H B,2018.Soil Organic Carbon,Carbon Sequestration,Soil microbial biomass carbon and nitrogen and soil enzymatic activity as influenced by conservation agriculture in pigeonpea and soybean intercropping system[J].International Journal of Current Microbiology and Applied Sciences,7(3):323-333.
LI D P,WU Z J,CHEN L J,2004.Dynamics of microbial biomass C in a black soil under long-term fertilization and related affecting factors[J].Chinese Journal of Applied Ecology,15(8):1334-1338.
NSABIMANA D,HAYNES R J,WALLIS F M,2004.Size,activity and catabolic diversity of the soil microbial biomass as affected by land use[J].Applied Soil Ecology,26:81-82.
ROD D B L,GRAEME J B,WAYNE M S,1993.Changes in soil organic matter with cropping as measured by organic carbon fractions and 13 Cnatural isotope abundance[J].Plant and Soil,155-156(1):399-402.
ROS G H,TEMMINGHOFF E J M,GROENIGEN J W V,et al.,2010.Isotopic analysis of dissolved organic nitrogen in soils[J].Analytical Chemistry,82(18):7814-7820.
XU X F,THORNTON P E,POST W M,2013.A global analysis of soil microbial biomass carbon,nitrogen and phosphorus in terrestrial ecosystems[J].Global Ecology and Biogeography,22(5-6):737-749.
宝日玛,峥嵘,周梅,等,2016.大兴安岭火烧迹地土壤微生物生物量及酶活性研究[J].内蒙古农业大学学(自然科学版),37(4):77-83.BAO R A,ZHENG R,ZHOU M,et al.,2016.Soil microbial biomass and enzyme activity of burned sites in greater hinggan mountains[J].Science of Inner Mongolia Agricultural University(Natural Science Edition),37(4):77-83.
鲍士旦,2000.土壤农化分析[M].3版.北京:中国农业出版社:25-96.BAO S D,2000.Soil agrochemical analysis[M].3 Ed.Beijing:China agriculture Press:25-96.
仇少君,彭佩钦,刘强,等,2006.土壤微生物生物量氮及其在氮素循环中作用[J].生态学杂志,25(4):443-448.QIU S J,PENG P Q,LIU Q,et al.,2006.Soil microbial biomass nitrogen and its role in nitrogen cycle[J].Ecology,25(4):443-448.
董莉丽,郑粉莉,安娟,2010.黄土丘陵不同土地利用类型下土壤微生物量特征[J].土壤通报,41(6):1371-1375.DONG L L,ZHENG F L,AN J,2010.Characteristics of soil microbial biomass under different land use types in loess hills[J].Chinese Soil Bulletin,41(6):1371-1375.
关松阴,1986.土壤酶及其研究方法[M].北京:农业出版社.GUAN S Y,1986.Soil enzyme and its research methods[M].Beijing:Agricultural Press.
韩永伟,韩建国,张蕴薇,等,2004.农牧交错带草地植被的水土保持作用研究[J].水土保持学报,18(4):24-28.HAN Y W,HAN J G,ZHANG Y W,et al.,2004.Study on soil and water conservation of grassland vegetation in farming-pastoral ecotone[J].Journal of Soil and Water Conservation,18(4):24-28.
贺美,王立刚,朱平,等,2017.长期定位施肥下黑土碳排放特征及其碳库组分与酶活性变化[J].生态学报,37(19):6379-6389.HE M,WANG L G,ZHU P,et al.,2017.Carbon emission characteristics,carbon sink components and enzyme activities of black soil under long-term fertilization[J].Acta Ecologica Sinica,37(19):6379-6389.
黄智鸿,赵海超,魏东,等,2016.不同施肥措施对春玉米农用地土壤酶活性的影响[J].中国土壤与肥料,265(5):18-24.HUANG Z H,ZHAO H C,WEI D,et al.,2016.Effects of different fertilization measures on soil enzyme activities in spring maize agricultural land[J].China Soil and Fertilizer,265(5):18-24.
霍利霞,红梅,赵巴音那木拉,等,2018.氮素和水分添加对荒漠草原土壤理化性质及微生物量碳氮的影响[J].北方农业学报,46(3):54-59.HUO L X,HONG M,ZHAO B Y N M L,et al.,2018.Effects of nitrogen and water addition on soil physical and chemical properties and microbial biomass of carbon and nitrogen in desert steppe[J].Journal of Northern Agriculture,46(3):54-59.
蒋永梅,师尚礼,田永亮,等,2017.高寒草地不同退化程度下土壤微生物及土壤酶活性变化特征[J].水土保持学报,31(3):244-249.JIANG Y M,SHI S L,TIAN Y L,et al.,2017.Changes of soil microorganism and soil enzyme activity in alpine grassland under different degrees of degradation[J].Journal of Soil and Water Conservation,31(3):244-249.
李生宝,王占军,王月玲,2006.宁南山区不同生态恢复措施对土壤环境效应影响的研究[J].水土保持学报,20(4):20-22.LI S B,WANG Z J,WANG Y L,2006.Study on effects of different ecological restoration measures on soil environment in ningnan mountain area[J].Journal of Soil and Water Conservation,20(4):20-22.
李亚娟,刘静,徐长林,等,2018.不同退化程度对高寒草甸土壤无机氮及脲酶活性的影响[J].草业学报,27(10):45-53.LI Y J,LIU J,XU C L,et al.,2018.Effects of different degrees of degradation on soil inorganic nitrogen and urease activity in alpine meadow[J].Journal of Grass Industry,27(10):45-53.
刘靓,2017.有机物料对复垦土壤有机碳含量及微生物活性的影响[D].太原:山西大学.LIU L,2017.Effects of organic materials on organic carbon content and microbial activity in reclaimed soil[D].Taiyuan:Shanxi University.
刘霓红,蒋先平,程俊峰,2018.国外有机设施园艺现状及对中国设施农业可持续发展的启示[J].农业工程学报,34(15):1-9.LIU N H,JIANG X P,CHENG J F,2018.The status quo of foreign organic facility horticulture and its enlightenment to the sustainable development of China's facility agriculture[J].Journal of Agricultural Engineering,34(15):1-9.
刘杨,2018.河南省森林土壤及微生物碳、氮、磷的生态化学计量特征[D].洛阳:河南科技大学.LIU Y,2018.Ecological stoichiometric characteristics of carbon,nitrogen and phosphorus in forest soil and microorganisms in henan province[D].Luoyang:Henan University of Science and Technology.
刘正恩,2010.河北坝上生态退化现状、原因及对策措施[J].生态经济(1):166-169.LIU Z E,2010.The current situation,causes of the ecological degradation in Bashang area of Hebei province and the countermeasures on them[J].Ecological Economics(1):166-169.
鲁如坤,1999.土壤农业化学分析方法[M].北京:中国农业科技出版社:106-110.LU L K,1999.Soil chemical analysis methods[M].Beijing:China Agricultural Science and Technology Press:106-110.
宁川川,王建武,蔡昆争,2016.有机肥对土壤肥力和土壤环境质量的影响研究进展[J].生态环境学报,25(1):175-181.NING C C,WANG J W,CAI K Z,2016.Research progress on the effect of organic fertilizers on soil fertility and soil environmental quality[J].Journal of Ecological Environment,25(1):175-181.
萐薛,李占斌,李鹏,等,2011.不同土地利用方式对干热河谷地区土壤酶活性的影响[J].中国农业科学,44(18):3768-3777.SHA X,LI Z B,LI P,et al.,2011.Effects of different land use patterns on soil enzyme activities in hot and dry river valleys[J].China Agricultural Science,44(18):3768-3777.
唐玉霞,贾树龙,孟春香,等,2002.土壤微生物生物量氮研究综述[J].中国生态农业学报,10(2):76-78.TANG Y X,JIA S L,NENG X C,et al.,2002.Review of soil microbial biomass nitrogen research[J].Chinese Journal of Ecological Agriculture,10(2):76-78.
王传杰,王齐齐,徐虎,等,2018.长期施肥下农田土壤-有机质-微生物的碳氮磷化学计量学特征[J].生态学报,38(11):3848-3858.WANG C J,WANG Q Q,XU H,et al.,2018.Carbon,nitrogen and phosphorus stoichiometric characteristics of soil-organic mattermicroorganisms under long-term fertilization[J].Acta Ecologica Sinica,38(11):3848-3858.
王丰,2008.武夷山不同海拔植被带土壤微生物量碳、氮、磷研究[D].南京:南京林业大学.WANG F,2008.Soil microbial biomass of carbon,nitrogen and phosphorus in vegetation zones at different elevations in wuyi mountain[D].Nanjing:Nanjing Forestry University.
王石英,蔡强国,吴淑安,2004.中国北方农牧交错区研究展望[J].水土保持研究,11(4):138-142.WANG S Y,CAI Q G,WU S A,2004.Prospects of agro-pastoral ecotone in north China[J].Research on Soil and Water Conservation,11(4):138-142.
王文锋,李春花,黄绍文,等,2016.不同施肥模式对设施菜田土壤微生物量碳、氮的影响[J].植物营养与肥料学报,22(5):1286-1297.WANG W F,LI C H,HUANG S W,et al.,2016.Effects of different fertilization modes on soil microbial biomass carbon and nitrogen in vegetable field[J].Chinese Journal of Plant Nutrition and Fertilizer,22(5):1286-1297.
王月,刘兴斌,韩晓日,等,2016.不同施肥处理对连作花生土壤微生物量和酶活性的影响[J].沈阳农业大学学报,47(5):553-558.WANG Y,LIU W B,HAN X R,et al.,2016.Effects of different fertilization treatments on soil microbial biomass and enzyme activity of continuous cropping peanut[J].Journal of Shenyang Agricultural University,47(5):553-558
吴金水,林启美,黄巧云,等,2006.土壤微生物量测定方法及应用[M].北京:气象出版社.WU J S,LIN Q M,HUANG Q Y,et al.,2006.Soil microbial biomass determination and its application[M].Beijing:Meteorological Press.
杨滨娟,黄国勤,钱海燕,2014.秸秆还田配施化肥对土壤温度、根际微生物及酶活性的影响[J].土壤学报,51(1):151-157.YANG B J,HUANG G Q,QIAN H Y,2014.Effects of straw mulching and fertilizer application on soil temperature,rhizosphere microorganism and enzyme activity[J].Acta Edologica Sinica,51(1):151-157.
杨雪艳,蒋代华,杨钙仁,等,2018.甘蔗水肥一体化种植对土壤微生物量碳氮和酶活性的影响[J].土壤通报,49(4):889-896.YANG X Y,JIANG D H,YANG G R,et al.,2018.Effects of sugarcane water and fertilizer integrated planting on soil microbial biomass,carbon and nitrogen and enzyme activities[J].Chinese Soil Science Bulletin,49(4):889-896.
尹亚丽,王玉琴,鲍根生,等,2017.退化高寒草甸土壤微生物及酶活性特征[J].应用生态学报,28(12):3881-3890.YIN Y L,WANG Y Q,BAO G S,et al.,2017.Characteristics of soil microorganism and enzyme activity in degraded alpine meadow[J].Chinese Journal of Applied Ecology,28(12):3881-3890.
张娟霞,刘伟刚,宁媛,等,2018.长期秸秆还田与施氮后土壤活性碳、氮的变化[J].水土保持学报,32(3):212-217.ZHANG J X,LIU W G,NING Y,et al.,2018.Changes of soil active carbon and nitrogen after long-term straw mulching and nitrogen application[J].Journal of Soil and Water Conservation,32(3):212-217.
张新荣,刘林萍,方石,等,2014.土地利用、覆被变化(LUCC)与环境变化关系研究进展[J].生态环境学报,23(12):2013-2021.ZHANG X R,LIU L P,FANG S,et al.,2014.Research progress on the relationship between land use,cover change(LUCC)and environmental change[J].Journal of Ecological Environment,23(12):2013-2021.
张知晓,泽桑梓,户连荣,等,2018.土壤脲酶活性调控因素和脲酶活性细菌系统发育研究[J].西部林业科学,47(1):65-73.ZHANG Z X,ZE S Z,HU L R,et al.,2018.Study on soil urease activity regulation factors and bacterial phylogeny of urease activity[J].Western Forestry Science,47(1):65-73.
赵海超,刘景辉,张星杰,等,2013.施肥对不同肥力水平春玉米农用地土壤有机碳及其组分的影响[J].生态环境学报,22(3):398-405.ZHAO H C,LIU J H,ZHANG X J,et al.,2013.Effects of fertilization on soil organic carbon and its components in spring maize agricultural land with different fertility levels[J].Journal of Ecological Environment,22(3):398-405.
赵彤,闫浩,蒋跃利,等,2013.黄土丘陵区植被类型对土壤微生物量碳氮磷的影响[J].生态学报,33(18):5615-5622.ZHAO T,YAN H,JIANG Y L,et al.,2013.Effects of vegetation types on soil microbial biomass of carbon,nitrogen and phosphorus in loess hilly regions[J].Acta Ecologica Sinica,33(18):5615-5622.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |